Mercurial > repos > iuc > edger
annotate edger.xml @ 8:3d89af8a44f0 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
| author | iuc |
|---|---|
| date | Thu, 03 Jun 2021 19:34:18 +0000 |
| parents | 334ce9b1bac5 |
| children | 6e53e565fc6a |
| rev | line source |
|---|---|
|
8
3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
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1 <tool id="edger" name="edgeR" version="3.34.0"> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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2 <description> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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3 Perform differential expression of count data |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
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4 </description> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
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5 <xrefs> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
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6 <xref type="bio.tools">edger</xref> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
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7 </xrefs> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
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8 <edam_topics> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
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9 <edam_topic>topic_3308</edam_topic> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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10 </edam_topics> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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11 <edam_operations> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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12 <edam_operation>operation_3563</edam_operation> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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changeset
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13 <edam_operation>operation_3223</edam_operation> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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changeset
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14 </edam_operations> |
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0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
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15 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
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16 <requirements> |
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8
3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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17 <requirement type="package" version="3.34.0">bioconductor-edger</requirement> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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18 <requirement type="package" version="3.48.0">bioconductor-limma</requirement> |
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4
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
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19 <requirement type="package" version="0.2.20">r-rjson</requirement> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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20 <requirement type="package" version="1.20.3">r-getopt</requirement> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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21 <requirement type="package" version="1.4.36">r-statmod</requirement> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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22 <!-- required for alpha function used with plotMD --> |
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3d89af8a44f0
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 215a0f27f3de87506895ac655f801c40e8c7edbc"
iuc
parents:
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diff
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23 <requirement type="package" version="1.1.1">r-scales</requirement> |
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0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
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24 </requirements> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
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25 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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26 <version_command><![CDATA[ |
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a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
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27 echo $(R --version | grep version | grep -v GNU)", edgeR version" $(R --vanilla --slave -e "library(edgeR); cat(sessionInfo()\$otherPkgs\$edgeR\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", limma version" $(R --vanilla --slave -e "library(limma); cat(sessionInfo()\$otherPkgs\$limma\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", scales version" $(R --vanilla --slave -e "library(scales); cat(sessionInfo()\$otherPkgs\$scales\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", rjson version" $(R --vanilla --slave -e "library(rjson); cat(sessionInfo()\$otherPkgs\$rjson\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", getopt version" $(R --vanilla --slave -e "library(getopt); cat(sessionInfo()\$otherPkgs\$getopt\$Version)" 2> /dev/null | grep -v -i "WARNING: ") |
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0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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28 ]]></version_command> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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29 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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30 <command detect_errors="exit_code"><![CDATA[ |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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31 #import json |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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32 Rscript '$__tool_directory__/edger.R' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
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33 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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34 -R '$outReport' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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35 -o '$outReport.files_path' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
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36 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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37 #if $input.format=="files": |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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38 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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39 ## Adapted from DESeq2 wrapper |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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40 #set $temp_factor_names = list() |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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41 #for $fact in $input.rep_factor: |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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42 #set $temp_factor = list() |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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43 #for $g in $fact.rep_group: |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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44 #set $count_files = list() |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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45 #for $file in $g.countsFile: |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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46 $count_files.append(str($file)) |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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47 #end for |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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48 $temp_factor.append( {str($g.groupName): $count_files} ) |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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49 #end for |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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50 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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51 $temp_factor.reverse() |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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52 $temp_factor_names.append([str($fact.factorName), $temp_factor]) |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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53 #end for |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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54 -j '#echo json.dumps(temp_factor_names)#' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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55 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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56 #elif $input.format=="matrix": |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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57 -m '$input.counts' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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58 #if $input.fact.ffile=='yes': |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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59 -f '$input.fact.finfo' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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60 #else: |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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61 -i '${ '|'.join( ['%s::%s' % ($x.factorName, $x.groupNames) for x in $input.fact.rep_factor] ) }' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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62 #end if |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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63 #end if |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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64 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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65 #if $anno.annoOpt=='yes': |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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66 -a '$anno.geneanno' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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67 #end if |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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68 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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69 -C '${ ','.join( ['%s' % $x.contrast for x in $rep_contrast] ) }' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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70 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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71 #if $f.filt.filt_select == 'yes': |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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72 #if $f.filt.cformat.format_select == 'cpm': |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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73 -c '$f.filt.cformat.cpmReq' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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74 -s '$f.filt.cformat.cpmSampleReq' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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75 #elif $f.filt.cformat.format_select == 'counts': |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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76 -z '$f.filt.cformat.cntReq' |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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77 #if $f.filt.cformat.samples.count_select == 'total': |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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78 -y |
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79 #elif $f.filt.cformat.samples.count_select == 'sample': |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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80 -s '$f.filt.cformat.samples.cntSampleReq' |
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9bdff28ae1b1
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81 #end if |
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9bdff28ae1b1
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82 #end if |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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83 #end if |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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84 |
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9bdff28ae1b1
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85 #if $out.normCounts: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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86 -x |
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9bdff28ae1b1
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87 #end if |
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88 #if $out.rdaOption: |
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89 -r |
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9bdff28ae1b1
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90 #end if |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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91 |
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92 -l '$adv.lfc' |
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93 -p '$adv.pVal' |
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94 -d '$adv.pAdjust' |
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95 -n '$adv.normalisationOption' |
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96 #if $adv.robOption: |
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97 -b |
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98 #end if |
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99 #if $adv.lrtOption: |
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100 -t |
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101 #end if |
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102 |
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103 && |
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104 mkdir ./output_dir |
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105 |
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106 && |
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107 cp '$outReport.files_path'/*.tsv output_dir/ |
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2
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108 |
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109 #if $out.rscript: |
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110 && cp '$__tool_directory__/edger.R' '$rscript' |
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111 #end if |
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0
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112 ]]></command> |
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113 |
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114 <inputs> |
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115 |
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116 <!-- Counts and Factors --> |
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117 <conditional name="input"> |
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118 <param name="format" type="select" label="Count Files or Matrix?" |
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9bdff28ae1b1
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119 help="You can choose to input either separate count files (one per sample) or a single count matrix"> |
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120 <option value="files">Separate Count Files</option> |
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121 <option value="matrix">Single Count Matrix</option> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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122 </param> |
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123 |
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124 <when value="files"> |
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125 <repeat name="rep_factor" title="Factor" min="1"> |
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6
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126 <param name="factorName" type="text" label="Name" help="Name of experiment factor of interest (e.g. Genotype). One factor must be entered and there must be two or more groups per factor. Optional additional factors (e.g. Batch) can be entered using the Insert Factor button below, see Help section for more information. NOTE: Please only use letters, numbers or underscores, and the first character of each factor must be a letter"> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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127 <sanitizer> |
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128 <valid initial="string.letters,string.digits"><add value="_" /></valid> |
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129 </sanitizer> |
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130 </param> |
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131 <repeat name="rep_group" title="Group" min="2" default="2"> |
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132 <param name="groupName" type="text" label="Name" |
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6
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133 help="Name of group that the counts files belong to (e.g. WT or Mut). NOTE: Please only use letters, numbers or underscores (case sensitive), and the first character of each group must be a letter"> |
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0
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134 <sanitizer> |
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135 <valid initial="string.letters,string.digits"><add value="_" /></valid> |
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136 </sanitizer> |
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137 </param> |
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3
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138 <param name="countsFile" type="data" format="tabular" multiple="true" label="Counts files"/> |
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0
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139 </repeat> |
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140 </repeat> |
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141 </when> |
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142 |
|
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143 <when value="matrix"> |
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144 <param name="counts" type="data" format="tabular" label="Count Matrix"/> |
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145 |
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146 <conditional name="fact"> |
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147 <param name="ffile" type="select" label="Input factor information from file?" |
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6
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148 help="You can choose to input the factor and group information for the samples from a file or manually enter below. NOTE: Please only use letters, numbers or underscores (case sensitive), and the first character of each sample, factor and group must be a letter"> |
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0
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149 <option value="no">No</option> |
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150 <option value="yes">Yes</option> |
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151 </param> |
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152 <when value="yes"> |
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153 <param name="finfo" type="data" format="tabular" label="Factor File"/> |
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9bdff28ae1b1
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154 </when> |
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155 <when value="no" > |
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156 <repeat name="rep_factor" title="Factor" min="1"> |
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9bdff28ae1b1
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157 <param name="factorName" type="text" label="Factor Name" |
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158 help="Name of experiment factor of interest (e.g. Genotype). One factor must be entered and there must be two or more groups per factor. Additional factors (e.g. Batch) can be entered using the Insert Factor button below, see Help section below. NOTE: Please only use letters, numbers or underscores, and the first character of each factor must be a letter"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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159 <validator type="empty_field" /> |
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160 <validator type="regex" message="Please only use letters, numbers or underscores">^[\w]+$</validator> |
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161 </param> |
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162 <param name="groupNames" type="text" label="Groups" |
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163 help="Enter the group names for the samples separated with commas e.g. WT,WT,WT,Mut,Mut,Mut. The order of the names must match the order of the samples in the columns of the count matrix. NOTE: Please only use letters, numbers or underscores (case sensitive), and the first character of each group must be a letter"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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164 <validator type="empty_field" /> |
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165 <validator type="regex" message="Please only use letters, numbers or underscores, and separate levels by commas">^[\w,]+$</validator> |
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166 </param> |
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167 </repeat> |
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168 </when> |
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169 </conditional> |
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170 </when> |
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171 </conditional> |
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172 |
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173 <!-- Gene Annotations --> |
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174 <conditional name="anno"> |
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175 <param name="annoOpt" type="select" label="Use Gene Annotations?" |
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176 help="If you provide an annotation file, annotations will be added to the table(s) of differential expression results to provide descriptions for each gene. See Help section below."> |
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177 <option value="no">No</option> |
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178 <option value="yes">Yes</option> |
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179 </param> |
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180 <when value="yes"> |
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181 <param name="geneanno" type="data" format="tabular" label="Gene Annotations"/> |
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182 </when> |
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183 <when value="no" /> |
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184 </conditional> |
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185 |
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186 <!-- Contrasts --> |
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187 <repeat name="rep_contrast" title="Contrast" min="1" default="1"> |
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188 <param name="contrast" type="text" label="Contrast of Interest" help="Names of two groups to compare separated by a hyphen e.g. Mut-WT. If the order is Mut-WT the fold changes in the results will be up/down in Mut relative to WT. If you have more than one contrast enter each separately using the Insert Contrast button below. For differences between contrasts use e.g. (MT.t1-MT.t0)-(WT.t1-WT.t0). For more info, see Chapter 8 in the limma User's guide: https://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst/doc/usersguide.pdf or https://bioconductor.org/packages/release/bioc/vignettes/edgeR/inst/doc/edgeRUsersGuide.pdf page 36 for nested comparisons."> |
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189 <validator type="empty_field" /> |
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190 <validator type="regex" message="Please only use letters, numbers, parentheses or underscores">^[\w\-()]+$</validator> |
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191 </param> |
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192 </repeat> |
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193 |
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194 <!-- Filter Options --> |
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195 <section name="f" expanded="false" title="Filter Low Counts"> |
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196 <conditional name="filt"> |
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197 <param name="filt_select" type="select" label="Filter lowly expressed genes?" help="Treat genes with very low expression as unexpressed and filter out. See the Filter Low Counts section below for more information. Default: No"> |
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198 <option value="no" selected="true">No</option> |
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199 <option value="yes">Yes</option> |
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200 </param> |
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201 <when value="yes"> |
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202 <conditional name="cformat"> |
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203 <param name="format_select" type="select" label="Filter on CPM or Count values?" help="It is slightly better to base the filtering on count-per-million (CPM) rather than the raw count values so as to avoid favoring genes expressed in samples sequenced to a higher depth. "> |
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204 <option value="cpm">CPM</option> |
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205 <option value="counts">Counts</option> |
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206 </param> |
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207 <when value="cpm"> |
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208 <param name="cpmReq" type="float" value="1" min="0" label="Minimum CPM" help="Treat genes with CPM below this value as unexpressed and filter out. See the Filter Low Counts section below for more information."/> |
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209 <param name="cpmSampleReq" type="integer" value="0" min="0" label="Minimum Samples" |
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210 help="Filter out all genes that do not meet the Minimum CPM in at least this many samples. See the Filter Low Counts section below for more information."/> |
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211 </when> |
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212 <when value="counts"> |
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213 <param name="cntReq" type="integer" value="0" min="0" label="Minimum Count" help="Filter out all genes that do not meet this minimum count. You can choose below to apply this filter to the total count for all samples or specify the number of samples under Minimum Samples. See the Filter Low Counts section below for more information." /> |
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214 <conditional name="samples"> |
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215 <param name="count_select" type="select" label="Filter on Total Count or per Sample Count values?" > |
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216 <option value="total">Total</option> |
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217 <option value="sample">Sample</option> |
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218 </param> |
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219 <when value="total"> |
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220 <param name="totReq" type="boolean" truevalue="1" falsevalue="0" checked="false" label="Filter on Total Count" help="Apply the Minimum Count filter to genes after summing counts for all samples. See the Filter Low Counts section below for more information." /> |
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221 </when> |
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222 <when value="sample"> |
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223 <param name="cntSampleReq" type="integer" value="0" min="0" label="Minimum Samples" |
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224 help="Filter out all genes that do not meet the Minimum Count in at least this many samples. See the Filter Low Counts section below for more information."/> |
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225 </when> |
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226 </conditional> |
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227 </when> |
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228 </conditional> |
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229 </when> |
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230 <when value="no" /> |
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231 </conditional> |
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232 </section> |
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233 |
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234 <!-- Output Options --> |
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235 <section name="out" expanded="false" title="Output Options"> |
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236 <param name="normCounts" type="boolean" truevalue="1" falsevalue="0" checked="false" |
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237 label="Output Normalised Counts Table?" |
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238 help="Output a file containing the normalised counts, these are in log2 counts per million (logCPM). Default: No"> |
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239 </param> |
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2
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240 <param name="rscript" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Output Rscript?" help="If this option is set to Yes, the Rscript used will be provided as a text file in the output. Default: No"/> |
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241 <param name="rdaOption" type="boolean" truevalue="1" falsevalue="0" checked="false" |
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242 label="Output RData file?" |
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243 help="Output all the data used by R to construct the plots and tables, can be loaded into R. A link to the RData file will be provided in the HTML report. Default: No"> |
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244 </param> |
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245 </section> |
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246 |
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247 <!-- Advanced Options --> |
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248 <section name="adv" expanded="false" title="Advanced Options"> |
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249 <param name="lfc" type="float" value="0" min="0" |
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250 label="Minimum Log2 Fold Change" |
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251 help="Genes above this threshold and below the p-value threshold are considered significant and highlighted in the MD plot. Default: 0."/> |
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252 <param name="pVal" type="float" value="0.05" min="0" max="1" |
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253 label="P-Value Adjusted Threshold" |
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254 help="Genes below this threshold are considered significant and highlighted in the MD plot. If either BH(1995) or BY(2001) are selected then this value is a false-discovery-rate control. If Holm(1979) is selected then this is an adjusted p-value for family-wise error rate. Default: 0.05."/> |
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255 <param name="pAdjust" type="select" label="P-Value Adjustment Method" help="Default: BH"> |
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256 <option value="BH" selected="true">Benjamini and Hochberg (1995)</option> |
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257 <option value="BY">Benjamini and Yekutieli (2001)</option> |
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258 <option value="holm">Holm (1979)</option> |
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259 <option value="none">None</option> |
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260 </param> |
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261 <param name="normalisationOption" type="select" label="Normalisation Method" help="Default: TMM"> |
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262 <option value="TMM" selected="true">TMM</option> |
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263 <option value="RLE">RLE</option> |
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264 <option value="upperquartile">Upperquartile</option> |
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265 <option value="none">None (Don't normalise)</option> |
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266 </param> |
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267 <param name="robOption" type="boolean" truevalue="1" falsevalue="0" checked="true" label="Use Robust Settings?" help="Using robust settings is usually recommended to protect against outlier genes. Default: Yes" /> |
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268 <param name="lrtOption" type="boolean" truevalue="1" falsevalue="0" checked="false" label="Use Likelihood Ratio Test?" help="Use likelihood ratio test instead of quasi-likelihood F-test. Default: No"/> |
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269 </section> |
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270 |
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271 </inputs> |
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272 |
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273 <outputs> |
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274 <data name="outReport" format="html" label="${tool.name} on ${on_string}: Report" /> |
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275 <collection name="outTables" type="list" label="${tool.name} on ${on_string}: Tables"> |
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276 <discover_datasets pattern="(?P<name>.+)\.tsv$" format="tabular" directory="output_dir" visible="false" /> |
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277 </collection> |
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2
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278 <data name="rscript" format="txt" label="${tool.name} on ${on_string}: Rscript"> |
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279 <filter>out['rscript']</filter> |
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280 </data> |
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0
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281 </outputs> |
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282 |
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283 <tests> |
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284 <!-- Ensure report is output --> |
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285 <test> |
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286 <param name="format" value="matrix" /> |
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287 <param name="counts" value="matrix.txt" /> |
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288 <repeat name="rep_factor"> |
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289 <param name="factorName" value="Genotype"/> |
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290 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT" /> |
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291 </repeat> |
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292 <repeat name="rep_contrast"> |
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293 <param name="contrast" value="Mut-WT" /> |
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294 </repeat> |
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295 <repeat name="rep_contrast"> |
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296 <param name="contrast" value="WT-Mut" /> |
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297 </repeat> |
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298 <param name="normalisationOption" value="TMM" /> |
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299 <output_collection name="outTables" count="2"> |
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2
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300 <element name="edgeR_Mut-WT" ftype="tabular" > |
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301 <assert_contents> |
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302 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR" /> |
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303 <has_text_matching expression="11304.*0.4582" /> |
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304 </assert_contents> |
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305 </element> |
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306 <element name="edgeR_WT-Mut" ftype="tabular" > |
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307 <assert_contents> |
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308 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR" /> |
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309 <has_text_matching expression="11304.*-0.4582" /> |
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310 </assert_contents> |
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a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
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1
diff
changeset
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311 </element> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff
changeset
|
312 </output_collection> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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changeset
|
313 <output name="outReport" > |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff
changeset
|
314 <assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
315 <has_text text="edgeR Analysis Output" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
316 <has_text text="quasi-likelihood" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
317 <not_has_text text="likelihood ratio" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff
changeset
|
318 <not_has_text text="RData" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff
changeset
|
319 </assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
320 </output> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
321 </test> |
|
7
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
|
322 <!-- Complex contrasts constructions --> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
6
diff
changeset
|
323 <test> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
6
diff
changeset
|
324 <param name="format" value="matrix" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
6
diff
changeset
|
325 <param name="counts" value="matrix-complex.txt" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
6
diff
changeset
|
326 <repeat name="rep_factor"> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
6
diff
changeset
|
327 <param name="factorName" value="Genotype"/> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
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diff
changeset
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328 <param name="groupNames" value="MutA,MutA,MutA,MutB,MutB,MutB,WTA,WTA,WTA,WTB,WTB,WTB" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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329 </repeat> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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330 <repeat name="rep_contrast"> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
|
331 <param name="contrast" value="(MutA-MutB)-(WTA-WTB)" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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332 </repeat> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
|
333 <param name="normalisationOption" value="TMM" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
|
334 <output_collection name="outTables" count="1"> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
6
diff
changeset
|
335 <element name="edgeR_(MutA-MutB)-(WTA-WTB)" ftype="tabular" > |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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336 <assert_contents> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
6
diff
changeset
|
337 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
6
diff
changeset
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338 <has_text_matching expression="11304.*15.53" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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339 </assert_contents> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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340 </element> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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341 </output_collection> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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342 <output name="outReport" > |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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343 <assert_contents> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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344 <has_text text="edgeR Analysis Output" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
|
345 <has_text text="quasi-likelihood" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
|
346 <not_has_text text="likelihood ratio" /> |
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334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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347 <not_has_text text="RData" /> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
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diff
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348 </assert_contents> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
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diff
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349 </output> |
|
334ce9b1bac5
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 106b877ec24a2422c0c13e37bf275c7441108b36
iuc
parents:
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diff
changeset
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350 </test> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff
changeset
|
351 <!-- Ensure annotation file input works --> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
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352 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff
changeset
|
353 <param name="format" value="matrix" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
354 <param name="annoOpt" value="yes" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
355 <param name="geneanno" value="anno.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
356 <param name="counts" value="matrix.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
357 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
358 <param name="factorName" value="Genotype"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
359 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
360 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
361 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
362 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
363 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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diff
changeset
|
364 <param name="normalisationOption" value="TMM" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
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365 <output_collection name="outTables" count="1"> |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
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366 <element name="edgeR_Mut-WT" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
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367 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
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diff
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368 <has_text_matching expression="EntrezID.*Symbol.*logFC.*logCPM.*F.*PValue.*FDR" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
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369 <has_text_matching expression="11304.*Abca4.*0.4582" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
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370 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
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changeset
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371 </element> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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changeset
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372 </output_collection> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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changeset
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373 </test> |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
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374 <!-- Ensure RScript and RData file can be output --> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
375 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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changeset
|
376 <param name="format" value="matrix" /> |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
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|
377 <param name="rscript" value="True"/> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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changeset
|
378 <param name="rdaOption" value="true" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
379 <param name="counts" value="matrix.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
380 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
381 <param name="factorName" value="Genotype"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
382 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
383 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
384 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
385 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
386 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
387 <param name="normalisationOption" value="TMM" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
388 <output name="outReport" > |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
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389 <assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
390 <has_text text="RData" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
391 </assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
392 </output> |
|
4
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
393 <output name="rscript"> |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
394 <assert_contents> |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
395 <has_text_matching expression="Task run time" /> |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
396 </assert_contents> |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
397 </output> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
398 </test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
399 <!-- Ensure secondary factors work --> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
400 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
401 <param name="format" value="matrix" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
402 <param name="counts" value="matrix.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
403 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
404 <param name="factorName" value="Genotype"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
405 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
406 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
407 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
408 <param name="factorName" value="Batch"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
409 <param name="groupNames" value="b1,b2,b3,b1,b2,b3"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
410 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
411 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
412 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
413 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
414 <param name="normalisationOption" value="TMM" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
415 <output_collection name="outTables" count="1" > |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
416 <element name="edgeR_Mut-WT" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
417 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
418 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
419 <has_text_matching expression="11304.*0.4584" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
420 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
421 </element> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
422 </output_collection> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
423 </test> |
|
4
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
424 <!-- Ensure factors file with unordered samples works --> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
425 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
426 <param name="format" value="matrix" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
427 <param name="ffile" value="yes" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
428 <param name="finfo" value="factorinfo.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
429 <param name="counts" value="matrix.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
430 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
431 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
432 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
433 <param name="normalisationOption" value="TMM" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
434 <output_collection name="outTables" count="1"> |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
435 <element name="edgeR_Mut-WT" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
436 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
437 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
438 <has_text_matching expression="11304.*0.4584" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
439 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
440 </element> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
441 </output_collection> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
442 </test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
443 <!-- Ensure normalised counts file output works--> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
444 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
445 <param name="format" value="matrix" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
446 <param name="normCounts" value="true" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
447 <param name="counts" value="matrix.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
448 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
449 <param name="factorName" value="Genotype"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
450 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
451 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
452 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
453 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
454 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
455 <param name="normalisationOption" value="TMM" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
456 <output_collection name="outTables" count="2"> |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
457 <element name="edgeR_Mut-WT" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
458 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
459 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
460 <has_text_matching expression="11304.*0.4582" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
461 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
462 </element> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
463 <element name="edgeR_normcounts" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
464 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
465 <has_text_matching expression="GeneID.*Mut1.*Mut2.*Mut3.*WT1.*WT2.*WT3" /> |
|
5
fb9b9f0f2f06
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 91bdcc6a5a46173be6546d590cdf3f311c2b1476
iuc
parents:
4
diff
changeset
|
466 <has_text_matching expression="11304.*15.75" /> |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
467 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
468 </element> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
469 </output_collection> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
470 </test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
471 <!-- Ensure likelihood ratio option works --> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
472 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
473 <param name="format" value="matrix" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
474 <param name="counts" value="matrix.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
475 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
476 <param name="factorName" value="Genotype"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
477 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
478 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
479 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
480 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
481 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
482 <param name="normalisationOption" value="TMM" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
483 <param name="lrtOption" value="true" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
484 <output name="outReport" > |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
485 <assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
486 <has_text text="likelihood ratio" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
487 <not_has_text text="quasi-likelihood" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
488 </assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
489 </output> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
490 </test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
491 <!-- Ensure multiple counts files input works --> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
492 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
493 <param name="format" value="files" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
494 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
495 <param name="factorName" value="Genotype"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
496 <repeat name="rep_group"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
497 <param name="groupName" value="WT"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
498 <param name="countsFile" value="WT1.counts,WT2.counts,WT3.counts"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
499 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
500 <repeat name="rep_group"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
501 <param name="groupName" value="Mut"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
502 <param name="countsFile" value="Mut1.counts,Mut2.counts,Mut3.counts"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
503 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
504 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
505 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
506 <param name="factorName" value="Batch"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
507 <repeat name="rep_group"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
508 <param name="groupName" value="b1"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
509 <param name="countsFile" value="WT1.counts,Mut1.counts"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
510 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
511 <repeat name="rep_group"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
512 <param name="groupName" value="b2"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
513 <param name="countsFile" value="WT2.counts,Mut2.counts"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
514 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
515 <repeat name="rep_group"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
516 <param name="groupName" value="b3"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
517 <param name="countsFile" value="WT3.counts,Mut3.counts"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
518 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
519 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
520 <param name="annoOpt" value="yes" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
521 <param name="geneanno" value="anno.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
522 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
523 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
524 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
525 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
526 <param name="contrast" value="WT-Mut" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
527 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
528 <param name="normCounts" value="true" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
529 <output_collection name="outTables" count="3"> |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
530 <element name="edgeR_Mut-WT" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
531 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
532 <has_text_matching expression="EntrezID.*Symbol.*logFC.*logCPM.*F.*PValue.*FDR" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
533 <has_text_matching expression="11304.*Abca4.*0.4584" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
534 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
535 </element> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
536 <element name="edgeR_WT-Mut" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
537 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
538 <has_text_matching expression="logFC.*logCPM.*F.*PValue.*FDR" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
539 <has_text_matching expression="11304.*Abca4.*-0.4584" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
540 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
541 </element> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
542 <element name="edgeR_normcounts" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
543 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
544 <has_text_matching expression="Mut1.*Mut2.*Mut3.*WT1.*WT2.*WT3" /> |
|
5
fb9b9f0f2f06
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 91bdcc6a5a46173be6546d590cdf3f311c2b1476
iuc
parents:
4
diff
changeset
|
545 <has_text_matching expression="11304.*Abca4.*15.75" /> |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
546 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
547 </element> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
548 </output_collection> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
549 </test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
550 <!-- Ensure filtering on CPM in Mnimum Samples works --> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
551 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
552 <param name="format" value="matrix" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
553 <param name="counts" value="matrix.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
554 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
555 <param name="factorName" value="Genotype"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
556 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
557 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
558 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
559 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
560 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
561 <param name="normalisationOption" value="TMM" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
562 <param name="filt_select" value="yes" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
563 <param name="format_select" value="cpm" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
564 <!-- real cpmReq values would be a lot lower |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
565 this is just for this tiny test dataset --> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
566 <param name="cpmReq" value="1000" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
567 <param name="cpmSampleReq" value="3" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
568 <output name="outReport" > |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
569 <assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
570 <has_text text="CPM in at least" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
571 <not_has_text text="after summing counts for all samples" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
572 <not_has_text text="counts in at least" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
573 </assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
574 </output> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
575 <output_collection name="outTables" count="1" > |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
576 <element name="edgeR_Mut-WT" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
577 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
578 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
579 <has_text_matching expression="11304.*0.4568" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
580 <not_has_text text="-0.0682" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
581 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
582 </element> |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
583 </output_collection> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
584 </test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
585 <!-- Ensure filtering on Count in Minmum Samples works --> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
586 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
587 <param name="format" value="matrix" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
588 <param name="counts" value="matrix.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
589 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
590 <param name="factorName" value="Genotype"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
591 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
592 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
593 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
594 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
595 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
596 <param name="normalisationOption" value="TMM" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
597 <param name="filt_select" value="yes" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
598 <param name="format_select" value="counts" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
599 <param name="cntReq" value="10" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
600 <param name="count_select" value="sample" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
601 <param name="cntSampleReq" value="3" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
602 <output name="outReport" > |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
603 <assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
604 <has_text text="counts in at least" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
605 <not_has_text text="after summing counts for all samples" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
606 <not_has_text text="CPM in at least" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
607 </assert_contents> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
608 </output> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
609 <output_collection name="outTables" count="1" > |
|
2
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
610 <element name="edgeR_Mut-WT" ftype="tabular" > |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
611 <assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
612 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
613 <has_text_matching expression="11304.*0.4568" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
614 <not_has_text text="-0.0682" /> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
615 </assert_contents> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
616 </element> |
|
a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
iuc
parents:
1
diff
changeset
|
617 |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
618 </output_collection> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
619 </test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
620 <!-- Ensure filtering on Total Count works --> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
621 <test> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
622 <param name="format" value="matrix" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
623 <param name="counts" value="matrix.txt" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
624 <repeat name="rep_factor"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
625 <param name="factorName" value="Genotype"/> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
626 <param name="groupNames" value="Mut,Mut,Mut,WT,WT,WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
627 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
628 <repeat name="rep_contrast"> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
629 <param name="contrast" value="Mut-WT" /> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
630 </repeat> |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
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631 <param name="normalisationOption" value="TMM" /> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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632 <param name="filt_select" value="yes" /> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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633 <param name="format_select" value="counts" /> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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634 <!-- real cntReq values would be a lot lower |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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635 this is just for this tiny test dataset --> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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636 <param name="cntReq" value="1000" /> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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637 <param name="count_select" value="total" /> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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638 <param name="totReq" value="true" /> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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639 <output name="outReport" > |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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640 <assert_contents> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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641 <has_text text="after summing counts for all samples" /> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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642 <not_has_text text="counts in at least" /> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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643 <not_has_text text="CPM in at least" /> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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644 </assert_contents> |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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645 </output> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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646 <output_collection name="outTables" count="1" > |
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2
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647 <element name="edgeR_Mut-WT" ftype="tabular" > |
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648 <assert_contents> |
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649 <has_text_matching expression="GeneID.*logFC.*logCPM.*F.*PValue.*FDR" /> |
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a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
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650 <has_text_matching expression="11304.*0.4568" /> |
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a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
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651 <not_has_text text="-0.0682" /> |
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a1634a9c2ee1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 910ffba48cb5f981aad1e00b77056bbbec7f9617
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652 </assert_contents> |
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653 </element> |
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0
9bdff28ae1b1
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654 </output_collection> |
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655 </test> |
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656 </tests> |
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657 |
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9bdff28ae1b1
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658 <help><![CDATA[ |
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659 .. class:: infomark |
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660 |
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9bdff28ae1b1
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661 **What it does** |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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662 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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663 Given a counts matrix, or a set of counts files, for example from **featureCounts**, and optional information about the genes, this tool |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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664 produces plots and tables useful in the analysis of differential gene expression. |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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665 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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666 This tool uses the `edgeR`_ quasi-likelihood pipeline (edgeR-quasi) for differential expression analysis. This statistical methodology uses negative binomial generalized linear models, but with F-tests instead of likelihood ratio tests. This method provides stricter error rate control than other negative binomial based pipelines, including the traditional edgeR pipelines or DESeq2. While the limma pipelines are recommended for large-scale datasets, because of their speed and flexibility, the edgeR-quasi pipeline gives better performance in low-count situations. For the data analyzed in this `edgeR workflow article`_ ,the edgeR-quasi, limma-voom and limma-trend pipelines are all equally suitable and give similar results. |
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9bdff28ae1b1
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667 |
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9bdff28ae1b1
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668 .. _edgeR: http://www.bioconductor.org/packages/release/bioc/html/edgeR.html |
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669 .. _edgeR workflow article: https://f1000research.com/articles/5-1438 |
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670 |
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9bdff28ae1b1
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671 ----- |
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9bdff28ae1b1
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672 |
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9bdff28ae1b1
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673 **Inputs** |
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9bdff28ae1b1
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674 |
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9bdff28ae1b1
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675 **Counts Data:** |
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9bdff28ae1b1
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676 |
|
6
555659de7321
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677 The counts data can either be input as separate counts files (one sample per file) or a single count matrix (one sample per column). The rows correspond to genes, and columns correspond to the counts for the samples. Values must be tab separated, with the first row containing the sample/column labels and the first column containing the row/gene labels. The sample labels must start with a letter. Gene identifiers can be of any type but must be unique and not repeated within a counts file. |
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678 |
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9bdff28ae1b1
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679 Example - **Separate Count Files**: |
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680 |
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9bdff28ae1b1
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681 ========== ======= |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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682 **GeneID** **WT1** |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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683 ---------- ------- |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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684 11287 1699 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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|
685 11298 1905 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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686 11302 6 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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687 11303 2099 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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688 11304 356 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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689 11305 2528 |
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690 ========== ======= |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
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691 |
|
9bdff28ae1b1
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692 Example - **Single Count Matrix**: |
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693 |
|
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694 ========== ======= ======= ======= ======== ======== ======== |
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9bdff28ae1b1
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695 **GeneID** **WT1** **WT2** **WT3** **Mut1** **Mut2** **Mut3** |
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696 ---------- ------- ------- ------- -------- -------- -------- |
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697 11287 1699 1528 1601 1463 1441 1495 |
|
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698 11298 1905 1744 1834 1345 1291 1346 |
|
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699 11302 6 8 7 5 6 5 |
|
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700 11303 2099 1974 2100 1574 1519 1654 |
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701 11304 356 312 337 361 397 346 |
|
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702 11305 2528 2438 2493 1762 1942 2027 |
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703 ========== ======= ======= ======= ======== ======== ======== |
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704 |
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705 |
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706 **Gene Annotations:** |
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707 Optional input for gene annotations, this can contain more |
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708 information about the genes than just an ID number. The annotations will |
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3
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709 be available in the differential expression results table and the optional normalised counts table. The file must contain a header row and have the gene IDs in the first column. The number of rows should match that of the counts files, add NA for any gene IDs with no annotation. The Galaxy tool **annotateMyIDs** can be used to obtain annotations for human, mouse, fly and zebrafish. |
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0
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710 |
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9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
711 Example: |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
712 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
713 ========== ========== =================================================== |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
714 **GeneID** **Symbol** **GeneName** |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
715 ---------- ---------- --------------------------------------------------- |
|
3
d79ed3ec25fe
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit e646be741e315df9332b5206cec1e47c11370ff1
iuc
parents:
2
diff
changeset
|
716 11287 Pzp pregnancy zone protein |
|
d79ed3ec25fe
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit e646be741e315df9332b5206cec1e47c11370ff1
iuc
parents:
2
diff
changeset
|
717 11298 Aanat arylalkylamine N-acetyltransferase |
|
d79ed3ec25fe
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit e646be741e315df9332b5206cec1e47c11370ff1
iuc
parents:
2
diff
changeset
|
718 11302 Aatk apoptosis-associated tyrosine kinase |
|
d79ed3ec25fe
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit e646be741e315df9332b5206cec1e47c11370ff1
iuc
parents:
2
diff
changeset
|
719 11303 Abca1 ATP-binding cassette, sub-family A (ABC1), member 1 |
|
d79ed3ec25fe
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit e646be741e315df9332b5206cec1e47c11370ff1
iuc
parents:
2
diff
changeset
|
720 11304 Abca4 ATP-binding cassette, sub-family A (ABC1), member 4 |
|
d79ed3ec25fe
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit e646be741e315df9332b5206cec1e47c11370ff1
iuc
parents:
2
diff
changeset
|
721 11305 Abca2 ATP-binding cassette, sub-family A (ABC1), member 2 |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
722 ========== ========== =================================================== |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
723 |
|
4
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
724 **Factor Information:** |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
725 Enter factor names and groups in the tool form, or provide a tab-separated file that has the names of the samples in the first column and one header row. The sample names must be the same as the names in the columns of the count matrix. The second column should contain the primary factor levels (e.g. WT, Mut) with optional additional columns for any secondary factors. |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
726 |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
727 Example: |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
728 |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
729 ========== ============ ========= |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
730 **Sample** **Genotype** **Batch** |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
731 ---------- ------------ --------- |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
732 WT1 WT b1 |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
733 WT2 WT b2 |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
734 WT3 WT b3 |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
735 Mut1 Mut b1 |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
736 Mut2 Mut b2 |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
737 Mut3 Mut b3 |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
738 ========== ============ ========= |
|
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
739 |
|
6
555659de7321
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 21f0bc6c64129122212a6d182fdf3e360617e629
iuc
parents:
5
diff
changeset
|
740 *Factor Name:* The name of the experimental factor being investigated e.g. Genotype, Treatment. One factor must be entered, the name should start with a letter and spaces must not be used. Optionally, additional factors can be included, these are variables that might influence your experiment e.g. Batch, Gender, Subject. If additional factors are entered, an additive linear model will be used. |
|
4
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
741 |
|
6
555659de7321
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 21f0bc6c64129122212a6d182fdf3e360617e629
iuc
parents:
5
diff
changeset
|
742 *Groups:* The names of the groups for the factor. The names should start with a letter, and only contain letters, numbers and underscores, other characters such as spaces and hyphens must not be used. If entered into the tool form above, the order must be the same as the samples (to which the groups correspond) are listed in the columns of the counts matrix, with the values separated by commas. |
|
4
4730985c816f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 230fc767e2e402ee460440afab0e348f2ccab179
iuc
parents:
3
diff
changeset
|
743 |
|
0
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
744 **Contrasts of Interest:** |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
745 The contrasts you wish to make between levels. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
746 A common contrast would be a simple difference between two levels: "Mut-WT" |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
747 represents the difference between the mutant and wild type genotypes. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
748 Multiple contrasts must be entered separately using the Insert Contrast button, spaces must not be used. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
749 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
750 **Filter Low Counts:** |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
751 Genes with very low counts across all libraries provide little evidence for differential expression. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
752 In the biological point of view, a gene must be expressed at some minimal level before |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
753 it is likely to be translated into a protein or to be biologically important. In addition, the |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
754 pronounced discreteness of these counts interferes with some of the statistical approximations |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
755 that are used later in the pipeline. These genes should be filtered out prior to further |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
756 analysis. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
757 As a rule of thumb, genes are dropped if they can’t possibly be expressed in all the samples |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
758 for any of the conditions. Users can set their own definition of genes being expressed. Usually |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
759 a gene is required to have a count of 5-10 in a library to be considered expressed in that |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
760 library. Users should also filter with count-per-million (CPM) rather than filtering on the |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
761 counts directly, as the latter does not account for differences in library sizes between samples. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
762 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
763 Option to ignore the genes that do not show significant levels of |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
764 expression, this filtering is dependent on two criteria: CPM/count and number of samples. You can specify to filter on CPM (Minimum CPM) or count (Minimum Count) values: |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
765 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
766 * **Minimum CPM:** This is the minimum count per million that a gene must have in at |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
767 least the number of samples specified under Minimum Samples. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
768 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
769 * **Minimum Count:** This is the minimum count that a gene must have. It can be combined with either Filter |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
770 on Total Count or Minimum Samples. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
771 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
772 * **Filter on Total Count:** This can be used with the Minimum Count filter to keep genes |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
773 with a minimum total read count. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
774 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
775 * **Minimum Samples:** This is the number of samples in which the Minimum CPM/Count |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
776 requirement must be met in order for that gene to be kept. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
777 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
778 If the Minimum Samples filter is applied, only genes that exhibit a CPM/count greater than the required amount in at least the number of samples specified will be used for analysis. Care should be taken to |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
779 ensure that the sample requirement is appropriate. In the case of an experiment |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
780 with two experimental groups each with two members, if there is a change from |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
781 insignificant CPM/count to significant CPM/count but the sample requirement is set to 3, |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
782 then this will cause that gene to fail the criteria. When in doubt simply do not |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
783 filter or consult the `edgeR workflow article`_ for filtering recommendations. |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
784 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
785 **Advanced Options:** |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
786 |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
787 By default error rate for multiple testing is controlled using Benjamini and |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
788 Hochberg's false discovery rate control at a threshold value of 0.05. However |
|
9bdff28ae1b1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit eac022c9c6e51e661c1513306b9fefdad673487d
iuc
parents:
diff
changeset
|
789 there are options to change this to custom values. |
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790 |
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791 * **Minimum log2-fold-change Required:** |
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792 In addition to meeting the requirement for the adjusted statistic for |
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793 multiple testing, the observation must have an absolute log2-fold-change |
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794 greater than this threshold to be considered significant, thus highlighted |
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795 in the MD plot. |
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796 |
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797 * **Adjusted Threshold:** |
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798 Set the threshold for the resulting value of the multiple testing control |
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799 method. Only observations whose statistic falls below this value is |
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800 considered significant, thus highlighted in the MD plot. |
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801 |
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802 * **P-Value Adjustment Method:** |
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803 Change the multiple testing control method, the options are BH(1995) and |
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804 BY(2001) which are both false discovery rate controls. There is also |
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805 Holm(1979) which is a method for family-wise error rate control. |
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806 |
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807 **Normalisation Method:** |
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808 The most obvious technical factor that affects the read counts, other than gene expression |
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809 levels, is the sequencing depth of each RNA sample. edgeR adjusts any differential expression |
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810 analysis for varying sequencing depths as represented by differing library sizes. This is |
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811 part of the basic modeling procedure and flows automatically into fold-change or p-value |
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812 calculations. It is always present, and doesn’t require any user intervention. |
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813 The second most important technical influence on differential expression is one that is less |
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814 obvious. RNA-seq provides a measure of the relative abundance of each gene in each RNA |
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815 sample, but does not provide any measure of the total RNA output on a per-cell basis. |
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816 This commonly becomes important when a small number of genes are very highly expressed |
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817 in one sample, but not in another. The highly expressed genes can consume a substantial |
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818 proportion of the total library size, causing the remaining genes to be under-sampled in that |
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819 sample. Unless this RNA composition effect is adjusted for, the remaining genes may falsely |
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820 appear to be down-regulated in that sample . The edgeR `calcNormFactors` function normalizes for RNA composition by finding a set of scaling factors for the library sizes that minimize the log-fold changes between the samples for most genes. The default method for computing these scale factors uses a trimmed mean of M values (TMM) between each pair of samples. We call the product of the original library size and the scaling factor the *effective library size*. The effective library size replaces the original library size in all downsteam analyses. TMM is the recommended method for most RNA-Seq data where the majority (more than half) of the genes are believed not differentially expressed between any pair of the samples. You can change the normalisation method under **Advanced Options** above. For more information, see the `calcNormFactors` section in the `edgeR User's Guide`_. |
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821 |
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822 **Robust Settings** |
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823 Option to use robust settings. Using robust settings (robust=TRUE) with the edgeR estimateDisp and glmQLFit functions is usually recommended to protect against outlier genes. This is turned on by default. Note that it is only used with the quasi-likelihood F test method. For more information, see the `edgeR workflow article`_. |
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824 |
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825 **Test Method** |
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826 Option to use the likelihood ratio test instead of the quasi-likelihood F test. For more information, see the `edgeR workflow article`_. |
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827 |
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828 .. _edgeR User's Guide: http://www.bioconductor.org/packages/release/bioc/html/edgeR.html |
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829 |
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830 ----- |
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831 |
|
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832 **Outputs** |
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833 |
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834 This tool outputs |
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835 |
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836 * a table of differentially expressed genes for each contrast of interest |
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837 * a HTML report with plots and additional information |
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838 |
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839 Optionally, under **Output Options** you can choose to output |
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840 |
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841 * a normalised counts table |
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2
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842 * the R script used by this tool |
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0
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843 * an RData file |
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844 |
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845 ----- |
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846 |
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847 **Citations** |
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848 |
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849 Please try to cite the appropriate articles when you publish results obtained using software, as such citation is the main means by which the authors receive credit for their work. For the edgeR method itself, please cite Robinson et al., 2010, and for this tool (which was developed from the Galaxy limma-voom tool) please cite Liu et al., 2015. |
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850 |
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851 ]]></help> |
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852 <citations> |
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853 <citation type="doi">10.1093/bioinformatics/btp616</citation> |
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854 <citation type="doi">10.1093/nar/gkv412</citation> |
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855 </citations> |
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856 </tool> |