annotate egsea.xml @ 0:a8a083193440 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
author iuc
date Thu, 25 Jan 2018 02:23:23 -0500
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a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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1 <tool id="egsea" name="EGSEA" version="1.6.0.0">
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2 <description> easy and efficient ensemble gene set testing</description>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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3 <requirements>
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4 <requirement type="package" version="1.6.0">bioconductor-egsea</requirement>
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5 <requirement type="package" version="1.4.4">r-optparse</requirement>
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6 <requirement type="package" version="0.2.15">r-rjson</requirement>
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7 </requirements>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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8 <version_command><![CDATA[
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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9 echo $(R --version | grep version | grep -v GNU)", EGSEA version" $(R --vanilla --slave -e "library(EGSEA); cat(sessionInfo()\$otherPkgs\$EGSEA\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", optparse version" $(R --vanilla --slave -e "library(optparse); cat(sessionInfo()\$otherPkgs\$optparse\$Version)" 2> /dev/null | grep -v -i "WARNING: ")", rjson version" $(R --vanilla --slave -e "library(rjson); cat(sessionInfo()\$otherPkgs\$rjson\$Version)" 2> /dev/null | grep -v -i "WARNING: ")
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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10 ]]></version_command>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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11 <command detect_errors="exit_code"><![CDATA[
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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12 ## EGSEA requires at least 2 threads
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13 SLOTS=\${GALAXY_SLOTS:-2};
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14 [ "\$SLOTS" -eq 1 ] && SLOTS=2;
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15
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16 mkdir ./report_dir &&
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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17 mkdir '$outReport.extra_files_path' &&
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18
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19 Rscript '$__tool_directory__/egsea.R'
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20
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21 --threads \$SLOTS
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22
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23 #if $input.format=="files":
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24
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25 ## Adapted from DESeq2 wrapper
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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26 #import json
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27 #set $temp_factor_names = list()
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28 #for $fact in $input.rep_factor:
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29 #set $temp_factor = list()
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30 #for $g in $fact.rep_group:
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31 #set $count_files = list()
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32 #for $file in $g.countsFile:
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33 $count_files.append(str($file))
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34 #end for
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35 $temp_factor.append( {str($g.groupName): $count_files} )
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36 #end for
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37
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38 $temp_factor.reverse()
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39 $temp_factor_names.append([str($fact.factorName), $temp_factor])
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40 #end for
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41 --filesPath '#echo json.dumps(temp_factor_names)#'
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42
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43 #elif $input.format=="matrix":
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44 --matrixPath '$input.counts'
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45 #if $input.fact.ffile=='yes':
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46 --factFile '$input.fact.finfo'
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47 #else:
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48 --factInput '${ '|'.join( ['%s::%s' % ($x.factorName, $x.groupNames) for x in $input.fact.rep_factor] ) }'
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49 #end if
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50 #end if
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51
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52 --contrastData '${ ','.join( ['%s' % $x.contrast for x in $rep_contrast] ) }'
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53
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54 --genes '$genes'
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55 --species $species
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56
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57 --base_methods $base_methods
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58 --msigdb $msigdb.msigdb_gsets
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59 --keggdb $keggdb.keggdb_gsets
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60 --gsdb $gsdb.gsdb_gsets
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61
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62 --display_top $advanced.display_top
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63 --min_size $advanced.min_size
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64 --fdr_cutoff $advanced.fdr_cutoff
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65 --combine_method $advanced.combine_method
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66 --sort_method $advanced.sort_method
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67
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68 --rdaOpt $advanced.rdaOpt
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69
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70 && cp ./report_dir/index.html '$outReport'
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71 && cp -r ./report_dir/* '$outReport.extra_files_path'
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72
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73 #if $advanced.rscriptOpt:
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74 && cp '$__tool_directory__/egsea.R' '$outRscript'
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75 #end if
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76
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77 ]]></command>
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78 <inputs>
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79
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80 <!-- Counts and Factors -->
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81 <conditional name="input">
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82 <param name="format" type="select" label="Count Files or Matrix?"
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83 help="You can choose to input either separate count files (one per sample) or a single count matrix">
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84 <option value="files">Separate Count Files</option>
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85 <option value="matrix">Single Count Matrix</option>
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86 </param>
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87
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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parents:
diff changeset
88 <when value="files">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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parents:
diff changeset
89 <repeat name="rep_factor" title="Factor" min="1">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
90 <param name="factorName" type="text" label="Name" help="Name of experiment factor of interest (e.g. Genotype). One factor must be entered and there must be two or more groups per factor. Optional additional factors (e.g. Batch) can be entered using the Insert Factor button below, see Help section for more information. NOTE: Please only use letters, numbers or underscores.">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
91 <sanitizer>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
92 <valid initial="string.letters,string.digits"><add value="_" /></valid>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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parents:
diff changeset
93 </sanitizer>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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parents:
diff changeset
94 </param>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
95 <repeat name="rep_group" title="Group" min="2" default="2">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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96 <param name="groupName" type="text" label="Name"
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
97 help="Name of group that the counts files(s) belong to (e.g. WT or Mut). NOTE: Please only use letters, numbers or underscores (case sensitive).">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
98 <sanitizer>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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parents:
diff changeset
99 <valid initial="string.letters,string.digits"><add value="_" /></valid>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
100 </sanitizer>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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101 </param>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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102 <param name="countsFile" type="data" format="tabular" multiple="true" label="Counts file(s)"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
103 </repeat>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
104 </repeat>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
105 </when>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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106
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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107 <when value="matrix">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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108 <param name="counts" type="data" format="tabular" label="Count Matrix"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
109
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
110 <conditional name="fact">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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parents:
diff changeset
111 <param name="ffile" type="select" label="Input factor information from file?"
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
112 help="You can choose to input the factor and group information for the samples from a file or manually enter below.">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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113 <option value="no">No</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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114 <option value="yes">Yes</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
115 </param>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
116 <when value="yes">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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117 <param name="finfo" type="data" format="tabular" label="Factor File"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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118 </when>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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119 <when value="no" >
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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120 <repeat name="rep_factor" title="Factor" min="1">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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121 <param name="factorName" type="text" label="Factor Name"
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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122 help="Name of experiment factor of interest (e.g. Genotype). One factor must be entered and there must be two or more groups per factor. Additional factors (e.g. Batch) can be entered using the Insert Factor button below, see Help section below. NOTE: Please only use letters, numbers or underscores.">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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123 <validator type="empty_field" />
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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124 <validator type="regex" message="Please only use letters, numbers or underscores">^[\w]+$</validator>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
125 </param>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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126 <param name="groupNames" type="text" label="Groups"
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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127 help="Enter the group names for the samples separated with commas e.g. WT,WT,WT,Mut,Mut,Mut. The order of the names must match the order of the samples in the columns of the count matrix. NOTE: Please only use letters, numbers or underscores (case sensitive).">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
128 <validator type="empty_field" />
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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129 <validator type="regex" message="Please only use letters, numbers or underscores, and separate levels by commas">^[\w,]+$</validator>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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130 </param>
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diff changeset
131 </repeat>
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132 </when>
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133 </conditional>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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134 </when>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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135 </conditional>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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136
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
137 <!-- Contrasts -->
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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138 <repeat name="rep_contrast" title="Contrast" min="1" default="1">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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139 <param name="contrast" type="text" label="Contrast of Interest" help="Names of two groups to compare separated by a hyphen e.g. Mut-WT. If the order is Mut-WT the fold changes in the results will be up/down in Mut relative to WT. If you have more than one contrast enter each separately using the Insert Contrast button below. For more info, see Chapter 8 in the limma User's guide: https://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst/doc/usersguide.pdf">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
140 <validator type="empty_field" />
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
141 <validator type="regex" message="Please only use letters, numbers or underscores">^[\w-]+$</validator>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
142 </param>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
143 </repeat>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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diff changeset
144
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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145 <param name="genes" type="data" format="tabular"
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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146 label="Symbols Mapping file"
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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147 help="A file of Entrez Gene IDs mapped to Gene symbols in the format shown in the Help section below."/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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148
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149 <param name="species" type="select" label="Species" help="Default: Human">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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150 <option value="human" selected="True">Human</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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151 <option value="mouse">Mouse</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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152 <option value="rat">Rat</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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153 </param>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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154
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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155 <param name="base_methods" type="select" display="checkboxes" multiple="True" min="3" label="Gene Set Testing Methods" help="Select at least 3 gene set testing methods">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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156 <option value="camera" selected="True">camera</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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157 <option value="safe">safe</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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158 <option value="gage">gage</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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159 <option value="zscore">zscore</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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160 <option value="gsva">gsva</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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161 <option value="globaltest" selected="True">globaltest</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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162 <option value="ora" selected="True">ora</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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163 <option value="ssgsea">ssgsea</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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164 <option value="padog">padog</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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165 <option value="plage">plage</option>
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166 <option value="fry">fry</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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167 <option value="roast">roast</option>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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168 </param>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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169
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170 <section name="msigdb" title="MSigDB Gene Sets" expanded="True">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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171 <param name="msigdb_gsets" type="select" display="checkboxes" optional="True" multiple="True" label="MSigDB Gene Set Collections" help="Choose any MSigDB Gene Set Collections you want to use. Default: H: hallmark gene sets">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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172 <option value="h" selected="True">H: hallmark gene sets</option>
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173 <option value="c1">C1: positional gene sets (human only)</option>
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174 <option value="c2">C2: curated gene sets</option>
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175 <option value="c3">C3: motif gene sets</option>
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176 <option value="c4">C4: computational gene sets</option>
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177 <option value="c5">C5: GO gene sets</option>
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178 <option value="c6">C6: oncogenic gene sets</option>
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179 <option value="c7">C7: immunologic gene sets</option>
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180 </param>
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181 </section>
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182
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183 <section name="keggdb" title="KEGG Pathways" expanded="True">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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184 <param name="keggdb_gsets" type="select" display="checkboxes" optional="True" multiple="True" label="KEGG Pathways" help="Choose any KEGG Pathways you want to use. Default: None">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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185 <option value="keggmet">Metabolism pathways</option>
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186 <option value="keggsig">Signalling pathways</option>
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187 <option value="keggdis">Disease pathways</option>
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188 </param>
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189 </section>
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190
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191 <section name="gsdb" title="GeneSetDB Gene Sets" expanded="True">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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192 <param name="gsdb_gsets" type="select" display="checkboxes" optional="True" multiple="True" label="GeneSigDB Gene Set Collections" help="Choose any GeneSetDB Gene Set Collections you want to use. Default: None">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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193 <option value="gsdbpath">Pathway collection</option>
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194 <option value="gsdbdis">Disease/Phenotype collection</option>
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195 <option value="gsdbdrug">Drug/Chemical collection</option>
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196 <option value="gsdbreg">Gene Regulation collection</option>
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197 <option value="gsdbgo">Gene Ontology collection</option>
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198 </param>
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199 </section>
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200
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201 <section name="advanced" title="Advanced Options">
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202 <param name="display_top" type="integer" value="5" min="1" max="20" label="Top Gene Sets to display" help="Set the number of top gene sets to display. Increasing this number increases the time to run, in order to generate the additional plots etc."/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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203 <param name="min_size" type="integer" min="0" value="2" label="Minimum Size of Gene Set" help="Minimum size of a gene set to be included in the analysis. Default: 2" />
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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204 <param name="fdr_cutoff" type="float" value="0.05" min="0" max="1" label="FDR cutoff" help="Cut-off threshold of differentially expressed genes used for the calculation of Significance Score and Regulation Direction. Default: 0.05"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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205 <param name="combine_method" type="select" label="Combine Method" help="Method to use to combine the p-values from the different gene set testing methods. Default: wilkinson">
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206 <option value="wilkinson" selected="True">wilkinson</option>
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207 <option value="fisher">fisher</option>
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208 <option value="average">average</option>
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209 <option value="logitp">logitp</option>
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210 <option value="sump">sump</option>
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211 <option value="sumz">sumz</option>
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212 <option value="votep">votep</option>
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213 <option value="median">median</option>
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214 </param>
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215 <param name="sort_method" type="select" label="Sort Method" help="Select method to sort the results. Any of EGSEA’s combined scores or the rankings from individual base methods can be used for sorting the results. Default: med.rank">
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216 <option value="p.adj">p.adj</option>
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217 <option value="p.value">p.value</option>
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218 <option value="vote.rank">vote.rank</option>
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219 <option value="avg.rank">avg.rank</option>
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220 <option value="med.rank" selected="True">med.rank</option>
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221 <option value="min.pvalue">min.pvalue</option>
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222 <option value="min.rank">min.rank</option>
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223 <option value="avg.logfc">avg.logfc</option>
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224 <option value="avg.logfc.dir">avg.logfc.dir</option>
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225 <option value="direction">direction</option>
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226 <option value="significance">significance</option>
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227 <option value="camera">camera</option>
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228 <option value="roast">roast</option>
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229 <option value="safe" >safe</option>
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230 <option value="gage">gage</option>
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231 <option value="padog">padog</option>
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232 <option value="plage">plage</option>
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233 <option value="zscore">zscore</option>
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234 <option value="gsva">gsva</option>
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235 <option value="ssgsea">ssgsea</option>
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236 <option value="globaltest">globaltest</option>
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237 <option value="ora">ora</option>
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238 <option value="fry">fry</option>
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239 </param>
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240 <param name="rscriptOpt" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Output Rscript?" help="If this option is set to Yes, the Rscript used will be provided as a text file in the output. Default: No"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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241 <param name="rdaOpt" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Output RData file?" help="Output all the data used by R in the analysis, can be loaded into R. Default: No" />
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242 </section>
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243 <param name="non_commercial_use" label="I certify that I am not using this tool for commercial purposes." type="boolean" truevalue="NON_COMMERCIAL_USE" falsevalue="COMMERCIAL_USE" checked="False">
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244 <validator type="expression" message="This tool is only available for non-commercial use.">value == True</validator>
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245 </param>
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246 </inputs>
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247
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248 <outputs>
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249 <data name="outReport" format="html" label="${tool.name} on ${on_string}: Report"/>
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250 <collection name="outTables" type="list" label="${tool.name} on ${on_string}: Tables">
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251 <discover_datasets pattern="(?P&lt;name&gt;.+)\.txt$" format="tabular" directory="report_dir/ranked-gene-sets-base" visible="false" />
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252 </collection>
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253 <data name="outRscript" format="txt" from_work_dir="*.txt" label="${tool.name} on ${on_string}: Rscript">
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254 <filter>advanced['rscriptOpt'] is True</filter>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
255 </data>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
256 <data name="outRdata" format="rdata" from_work_dir="EGSEA_analysis.RData" label="${tool.name} on ${on_string}: RData file">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
257 <filter>advanced['rdaOpt'] is True</filter>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
258 </data>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
259 </outputs>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
260
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
261 <tests>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
262 <!-- Ensure report is output -->
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
263 <test expect_num_outputs="1">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
264 <param name="non_commercial_use" value="True"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
265 <param name="format" value="matrix" />
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
266 <param name="counts" value="il13.counts"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
267 <param name="genes" value="il13.genes"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
268 <repeat name="rep_factor">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
269 <param name="factorName" value="Treatment"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
270 <param name="groupNames" value="IL13,IL13Ant,IL13,IL13,IL13Ant"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
271 </repeat>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
272 <repeat name="rep_contrast">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
273 <param name="contrast" value="IL13Ant-IL13"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
274 </repeat>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
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275 <output_collection name="outTables" count="1">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
276 <element name="ranked-h-gene-sets-IL13Ant-IL13" ftype="tabular" file="ranked-h-gene-sets-IL13Ant-IL13.txt"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
277 </output_collection>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
278 <output name="outReport">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
279 <assert_contents>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
280 <has_text text="Gene Set Testing Report"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
281 </assert_contents>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
282 </output>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
283 </test>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
284 <!-- Ensure factors file input works and Rscript is output-->
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
285 <test expect_num_outputs="2">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
286 <param name="non_commercial_use" value="True"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
287 <param name="format" value="matrix"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
288 <param name="counts" value="il13.counts"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
289 <param name="genes" value="il13.genes"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
290 <param name="ffile" value="yes"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
291 <param name="finfo" value="il13.group"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
292 <repeat name="rep_contrast">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
293 <param name="contrast" value="IL13Ant-IL13" />
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
294 </repeat>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
295 <param name="rscriptOpt" value="True"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
296 <output name="outReport">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
297 <assert_contents>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
298 <has_text text="Gene Set Testing Report"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
299 </assert_contents>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
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diff changeset
300 </output>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
301 <output_collection name="outTables" count="1">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
302 <element name="ranked-h-gene-sets-IL13Ant-IL13" ftype="tabular" file="ranked-h-gene-sets-IL13Ant-IL13.txt"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
303 </output_collection>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
304 <output name="outRscript" value="out_rscript.txt"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
305 </test>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
306 <!-- Ensure two contrasts works -->
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
307 <test expect_num_outputs="1">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
308 <param name="non_commercial_use" value="True"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
309 <param name="format" value="matrix"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
310 <param name="counts" value="il13.counts"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
311 <param name="genes" value="il13.genes"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
312 <param name="ffile" value="yes"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
313 <param name="finfo" value="il13.group"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
314 <repeat name="rep_contrast">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
315 <param name="contrast" value="IL13Ant-IL13"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
316 </repeat>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
317 <repeat name="rep_contrast">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
318 <param name="contrast" value="IL13-IL13Ant"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
319 </repeat>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
320 <output_collection name="outTables" count="3">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
321 <element name="ranked-h-gene-sets-IL13Ant-IL13" ftype="tabular" file="ranked-h-gene-sets-IL13Ant-IL13.txt"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
322 <element name="ranked-h-gene-sets-IL13-IL13Ant" ftype="tabular" file="ranked-h-gene-sets-IL13-IL13Ant.txt"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
323 <element name="ranked-h-gene-sets-IL13-IL13Ant" ftype="tabular" file="ranked-h-gene-sets-compare.txt"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
324 </output_collection>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
325 </test>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
326 <!-- Ensure two factors works -->
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
327 <test expect_num_outputs="1">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
328 <param name="non_commercial_use" value="True"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
329 <param name="format" value="matrix"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
330 <param name="counts" value="il13.counts"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
331 <param name="genes" value="il13.genes"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
332 <param name="ffile" value="yes"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
333 <param name="finfo" value="il13.group_batch"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
334 <repeat name="rep_contrast">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
335 <param name="contrast" value="IL13Ant-IL13"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
336 </repeat>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
337 <output_collection name="outTables" count="1">
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
338 <element name="ranked-h-gene-sets-IL13Ant-IL13" ftype="tabular" file="ranked-h-gene-sets-IL13Ant-IL13_batch.txt"/>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
339 </output_collection>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
diff changeset
340 </test>
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
iuc
parents:
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341 </tests>
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342
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343 <help><![CDATA[
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344
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345 .. class:: infomark
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346
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347 **What it does**
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348
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349 EGSEA_, an acronym for *Ensemble of Gene Set Enrichment Analyses*, is a `Bioconductor package`_ that utilizes the analysis results of eleven prominent GSE algorithms from the literature to calculate collective significance scores for gene sets. These methods are currently: **ora, globaltest, plage, safe, zscore, gage, ssgsea, roast, fry, padog, camera, gsva**. The ora, gage, camera and gsva methods depend on a competitive null hypothesis while the remaining seven methods are based on a self-contained hypothesis. EGSEA’s gene set database, the **EGSEAdata** Bioconductor package, contains around 25,000 gene sets from 16 collections from MSigDB_, KEGG_ and GeneSetDB_. Supported organisms are human, mouse and rat, however MSigDB is only available for human and mouse. An example `EGSEA workflow`_ is available at the Bioconductor workflows website.
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350
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351 Currently the **egsea.cnt** function is implemented in this tool. This function takes a raw RNA-Seq count matrix and uses **limma-voom** with TMM normalization to convert the RNA-seq counts into expression values for EGSEA analysis.
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352
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353 EGSEA returns a HTML report of detailed analysis results for each contrast of interest and comparative analysis results. The heatmap view at both the gene set and summary level and the summary level bar plots can be useful summaries to include in publications to highlight the gene set testing results.
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354
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355 .. class:: warningmark
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356
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357 **WARNING: This tool is only available for non-commercial use.**
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358
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359 The **GAGE** and **Pathview** packages used by EGSEA make use of KEGG data
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360 and Non-academic uses may require a KEGG license agreement. Before using, be
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361 sure to review, agree, and comply with the relevant licenses for KEGG and
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362 MSigDB.
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363
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364 * `KEGG Licence`_
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365 * `MSigDB Licence`_
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366
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367 .. _KEGG Licence: http://www.kegg.jp/kegg/legal.html
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368 .. _MSigDB Licence: http://software.broadinstitute.org/gsea/license_terms_list.jsp
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369
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370 -----
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371
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372 **Inputs**
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373
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374 **Counts Matrix**
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375
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376 This tool requires a counts matrix (counts table) containing the raw RNA-seq read counts. The first column must contain Entrez Gene IDs that are unique (not repeated) within the counts file. Entrez IDs can be obtained from the **annotateMyIDs** Galaxy tool.
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377
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378 Example:
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379
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380 =============== ========== ========== ========== ========= ========= =========
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381 EntrezID **WT1** **WT2** **WT3** **Mut1** **Mut2** **Mut3**
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382 =============== ========== ========== ========== ========= ========= =========
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383 1 71 73 69 36 22 28
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384 1000 3 4 2 4 0 1
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385 10000 2310 2142 2683 1683 2068 2172
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386 100009605 3 1 2 1 5 3
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387 100009613 9 11 4 13 6 10
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388 =============== ========== ========== ========== ========= ========= =========
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389
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390 **Factor Information**
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391
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392 Enter factor names and groups in the tool form, or provide a tab-separated file that has the samples in the same order as listed in the columns of the counts matrix. The second column should contain the primary factor levels (e.g. WT, Mut) with optional additional columns for any secondary factors e.g Batch.
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393
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394 Example:
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395
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396 ========== ============ =========
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397 **Sample** **Genotype** **Batch**
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398 ---------- ------------ ---------
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399 WT1 WT b1
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400 WT2 WT b2
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401 WT3 WT b3
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402 Mut1 Mut b1
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403 Mut2 Mut b2
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404 Mut3 Mut b3
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405 ========== ============ =========
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406
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407 *Factor Name:* The name of the experimental factor being investigated e.g. Genotype, Treatment. One factor must be entered and spaces must not be used. Optionally, additional factors can be included, these are variables that might influence your experiment e.g. Batch, Gender, Subject. If additional factors are entered, edgeR will fit an additive linear model.
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408
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409 *Groups:* The names of the groups for the factor. These must be entered in the same order as the samples (to which the groups correspond) are listed in the columns of the counts matrix. Spaces must not be used and if entered into the tool form above, the values should be separated by commas.
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410
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411 **Symbols Mapping file**
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412
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413 A file containing the Gene Symbol for each Entrez Gene ID. The first column must be the Entrez Gene IDs and the second column must be the Gene Symbols. It is used for the heatmap visualization. The number of rows should match that of the Counts Matrix.
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414
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415 Example:
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416
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417 ========= =========
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418 EntrezID Symbols
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419 ========= =========
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420 1 A1BG
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421 1000 CDH2
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422 10000 AKT3
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423 100009605 TRNAF1
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424 100009613 ANO1-AS2
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425 ========= =========
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426
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427 -----
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428
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429 **Outputs**
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430
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431 The EGSEA report is an interactive HTML report that is generated to enable a swift navigation through the results of an EGSEA analysis. The pages below are generated for each gene set collection and contrast/comparison.
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432
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433 **Stats Table page**
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434
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435 The Stats Table page shows the detailed statistics of the EGSEA analysis for the top gene sets. It shows the EGSEA scores, individual rankings and additional annotation for each gene set. Hyperlinks to the source of each gene set can be seen in this table when they are available. The "Direction" column shows the regulation direction of a gene set which is calculated based on the logFC, which is either calculated from the limma differential expression analysis or provided by the user. The logFC cutoff and FDR cutoff are applied for this calculation. The calculations of the EGSEA scores can be seen in the references section. The method topSets can be used to generate custom Stats Table.
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436
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437 **Heatmaps page**
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438
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439 The Heatmaps page shows the heatmaps of the gene fold changes for the gene sets that are presented in the Stats Table page. Red indicates up-regulation while blue indicates down-regulation. Only genes that appear in the input expression/count matrix are visualized in the heat map. Gene names are coloured based on their statistical significance in the limma differential expression analysis. The "Interpret Results" link below each heat map allows the user to download the original heat map values along with additional statistics from limma DE analysis ( if available) so that they can be used to perform further analysis in R, e.g., customizing the heat map visualization.
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440
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441 **Summary Plots page**
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442
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443 The Summary Plots page shows the methods ranking plot along with the summary plots of EGSEA analysis. The method plot uses multidimensional scaling (MDS) to visualize the ranking of individual methods on a given gene set collection. The summary plots are bubble plots that visualize the distribution of gene sets based on the EGSEA Significance Score and another EGSEA score (default, p-value). Two summary plots are generated: ranking and directional plots. Each gene set is reprersented with a bubble which is coloured based on the EGSEA ranking (in ranking plots ) or gene set regulation direction (in directional plots) and sized based on the gene set cardinality (in ranking plots) or EGSEA Significance score (in directional plots). Since the EGSEA "Significance Score" is proportional to the p-value and the absolute fold changes, it could be useful to highlight gene sets that have high Significance scores. The blue labels on the summary plot indicate gene sets that do not appear in the top 10 list of gene sets based on the "sort.by" argument (black labels) yet they appear in the top 5 list of gene sets based on the EGSEA "Significance Score". If two contrasts are provided, the rank is calculated based on the "comparison" analysis results and the "Significance Score" is calculated as the mean.
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444
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445 **Pathways page**
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446
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447 The Pathways page shows the KEGG pathways for the gene sets that are presented in the Stats Table of a KEGG gene set collection. The gene fold changes are overlaid on the pathway maps and coloured based on the gene regulation direction: blue for down-regulation and red for up-regulation. Note that this page only appears if a KEGG gene set collection is used in the EGSEA analysis.
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448
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449 **GO Graphs page**
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450
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451 The GO Graphs page shows the Gene Ontology graphs for top 5 GO terms in each of three GO categories: Biological Processes (BP), Molecular Functions (MF), and Cellular Components (CC). Nodes are coloured based on the default sort.by score where red indicates high significance and yellow indicates low significance. Note that this page only appears if a Gene Ontology gene set collection is used, i.e., for the c5 collection from MSigDB or the gsdbgo collection from GeneSetDB.
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452
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453 **Interpret Results link**
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454
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455 The Interpret Results hyperlink in the EGSEA report allows the user to download the fold changes and limma analysis results and thus improve the interpretation of the results.
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456
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457 .. class:: warningmark
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458
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459 Note that the running time of this tool depends on a number of things, including the number of samples and contrasts provided as input, and also the number of gene set testing methods and gene set collections chosen. For example, the `egsea.cnt example`_ in the EGSEA vignette was conducted with 8 samples and 2 contrasts, using the KEGG Signaling and Disease pathways, and 7 of the 12 gene set testing methods, on a MacBook Pro machine that had a 2.8 GHz Intel Core i7 CPU and 16 GB of RAM. The execution time took 145.5 seconds using 16 threads.
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460
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461 .. _egsea.cnt example: https://bioconductor.org/packages/release/bioc/vignettes/EGSEA/inst/doc/EGSEA.pdf
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462
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463 -----
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464
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465 **More Information**
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466
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467 **MSigDB Gene Set Colletions**
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468
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469 The MSigDB_ gene sets are divided into 8 major collections:
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470
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471 * **H: hallmark gene sets** are coherently expressed signatures derived by aggregating many MSigDB gene sets to represent well-defined biological states or processes.
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472 * **C1: positional gene sets** for each human chromosome and cytogenetic band.
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473 * **C2: curated gene sets** are from online pathway databases, publications in PubMed, and knowledge of domain experts.
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474 * **C3: motif gene sets** are based on conserved cis-regulatory motifs from a comparative analysis of the human, mouse, rat, and dog genomes.
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475 * **C4: computational gene sets** are defined by mining large collections of cancer-oriented microarray data.
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476 * **C5: GO gene sets** consist of genes annotated by the same GO terms.
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477 * **C6: oncogenic gene sets** are defined directly from microarray gene expression data from cancer gene perturbations.
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478 * **C7: immunologic gene sets** are defined directly from microarray gene expression data from immunologic studies.
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479
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480 -----
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481
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482 **GeneSetDB Gene Set Colletions**
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483
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484 GeneSetDB_ gene sets were obtained from `multiple source databases`_ (shown below) and were classified into five subclasses based on the database content: Pathway, Disease/Phenotype, Drug/Chemical, Genes Regulation and Gene Ontology.
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485
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486 **Pathway**
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487
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488 * Biocarta
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489 * EHMN (Edinburgh Human Metabolic Network)
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490 * HumnCyc
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491 * INOH (Integrating Network Objects with Hierarchies)
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492 * NetPath
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493 * PID (Pathway Interaction Database)
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494 * Reactome
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495 * Wikipathways
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496
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497 **Disease/Phenotype**
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498
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499 * CancerGenes
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500 * KEGG Disease
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501 * HPO (Human Phenotype Ontology)
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502 * MethCancerDB
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503 * MethyCancer
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504 * MPO (Mammalian Phenotype Ontology)
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505 * SIDER (SIDe Effect Resource)
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506
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507 **Drug/Chemical**
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508
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509 * CTD (Comparative Toxicogenomics Database)
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510 * DrugBank
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511 * MATADOR (Manually Annotated Targets and Drugs Online Resource)
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512 * SMPDB (Small Molecular Pathway DataBase)
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513 * STITCH (Search Tool for Interactions of Chemicals)
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514 * T3DB (Toxin and Toxin Target Database)
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515
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516 **Gene Regulation**
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517
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518 * MicroCosm Targets
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519 * miRTarBase
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520 * TFactS
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521 * Rel/NF-kappaB target genes
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522
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523 **Gene Ontology**
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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524
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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525 * Gene Ontology
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526
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527 -----
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528
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529 **KEGG Pathways**
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530
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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531 Obtained by EGSEAdata from the GAGE_ Bioconductor package using the gage function kegg.gsets(). The Pathview_ Bioconductor package is used to visualize the expression data mapped onto the KEGG pathway graphs. Pathview has a GPLv3 licence which means users are required to formally cite the original `Pathview paper`_ (not just mention it) in publications or products. GAGE/Pathview divide the KEGG pathways into 3 categories: Signaling, Metabolism and Disease, listed in this file at the `Pathview website here`_.
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532
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533 **Signaling**
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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534
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535 * Genetic Information Processing
a8a083193440 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/egsea commit 7d0c7d850cd56ea3e54d8c03266f719241b20b8b
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536 * Environmental Information Processing
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537 * Cellular Processes
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538 * Organismal Systems
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539
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540 **Metabolism**
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541
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542 * Metabolism
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543
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544 **Disease**
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545
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546 * Human Diseases
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547
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548 -----
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549
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550 Please cite EGSEA_, MSigDB_, KEGG_ and GeneSetDB_ appropriately if you use them.
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551
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552 .. _EGSEA: https://www.ncbi.nlm.nih.gov/pubmed/27694195
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553 .. _Bioconductor package: https://bioconductor.org/packages/release/bioc/html/EGSEA.html
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554 .. _MSigDB: http://software.broadinstitute.org/gsea/msigdb
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555 .. _KEGG: http://www.genome.jp/kegg/
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556 .. _GeneSetDB: http://genesetdb.auckland.ac.nz/haeremai.html
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557 .. _EGSEA workflow: https://www.bioconductor.org/help/workflows/EGSEA123/
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558 .. _multiple source databases: http://genesetdb.auckland.ac.nz/sourcedb.html
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559 .. _GAGE: https://bioconductor.org/packages/release/bioc/html/gage.html
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560 .. _Pathview: https://bioconductor.org/packages/release/bioc/html/pathview.html
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561 .. _Pathview paper: https://www.ncbi.nlm.nih.gov/pubmed/23740750
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562 .. _Pathview website here: https://pathview.uncc.edu/data/khier.tsv
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563
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564 ]]></help>
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565 <citations>
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566 <citation type="doi">10.1093/bioinformatics/btw623</citation>
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567 </citations>
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568 </tool>