Mercurial > repos > iuc > fastp
annotate fastp.xml @ 9:dbf9c561ef29 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
author | iuc |
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date | Tue, 21 Apr 2020 16:45:20 -0400 |
parents | 1d8fe9bc4cb0 |
children | c59d48774d03 |
rev | line source |
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9
dbf9c561ef29
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
iuc
parents:
8
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1 <tool id="fastp" name="fastp" version="@WRAPPER_VERSION@+galaxy0"> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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2 <description>- fast all-in-one preprocessing for FASTQ files</description> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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3 <macros> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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4 <import>macros.xml</import> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
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5 </macros> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
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6 <requirements> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
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7 <requirement type="package" version="@WRAPPER_VERSION@">fastp</requirement> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
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8 </requirements> |
3
e0b44bf2543e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit d4f5a03011790c36ffd0f698b1e75c80f87fd1bf
iuc
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9 <version_command>fastp -v</version_command> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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10 <command detect_errors="exit_code"><![CDATA[ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 761a954744119f6317afe28eb50bd4f3fdea4984
iuc
parents:
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11 #import re |
7c82cb2a90c4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 761a954744119f6317afe28eb50bd4f3fdea4984
iuc
parents:
3
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12 |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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13 ## Link input files |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
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14 |
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3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
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15 #set ext = '.fastq' |
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988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
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16 |
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3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
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17 #if $single_paired.single_paired_selector == 'paired_collection': |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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18 #if $single_paired.paired_input.forward.is_of_type('fastq.gz'): |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
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19 #set ext = '.fastq.gz' |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
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20 #end if |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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21 #set $in1 = $single_paired.paired_input.forward |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
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22 #set $in2 = $single_paired.paired_input.reverse |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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23 #set $in1_name = re.sub('[^\w\-\s]', '_', str($single_paired.paired_input.name)) + $ext |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
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24 #set $in2_name = re.sub('[^\w\-\s]', '_', str("%s_%s" % ($single_paired.paired_input.name, "R2"))) + $ext |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
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25 #set out1 = $output_paired_coll.forward |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
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26 #set out2 = $output_paired_coll.reverse |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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27 ln -s '$in1' '$in1_name' && |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
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28 ln -s '$in2' '$in2_name' && |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
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29 #else |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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30 #if $in1.is_of_type('fastq.gz') |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
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31 #set ext = '.fastq.gz' |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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32 #end if |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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33 |
5
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
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34 #set $in1_name = re.sub('[^\w\-\s]', '_', str($in1.element_identifier)) + $ext |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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35 ln -s '$in1' '$in1_name' && |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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36 |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
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37 #if str($single_paired.single_paired_selector) == 'paired': |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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38 #set $in2_name = re.sub('[^\w\-\s]', '_', str("%s_R2" % $in2.element_identifier)) + $ext |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
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39 ln -s '$in2' '$in2_name' && |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
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40 #end if |
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988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
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41 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
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42 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
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43 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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44 ## Run fastp |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
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45 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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46 fastp |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
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47 |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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48 --thread \${GALAXY_SLOTS:-1} |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 761a954744119f6317afe28eb50bd4f3fdea4984
iuc
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49 --report_title 'fastp report for $in1_name' |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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50 |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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51 #if $in1.is_of_type('fastqillumina', 'fastqsolexa', 'fastqillumina.gz', 'fastqsolexa.gz'): |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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52 --phred64 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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53 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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54 |
5
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
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55 -i '$in1_name' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 761a954744119f6317afe28eb50bd4f3fdea4984
iuc
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56 -o first${ext} |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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57 |
5
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
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58 #if str($single_paired.single_paired_selector).startswith('paired'): |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
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59 -I '$in2_name' |
4
7c82cb2a90c4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 761a954744119f6317afe28eb50bd4f3fdea4984
iuc
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60 -O second${ext} |
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988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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61 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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62 |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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63 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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64 ## Adapter Trimming Options |
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988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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65 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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66 $single_paired.adapter_trimming_options.disable_adapter_trimming |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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67 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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68 #if str($single_paired.adapter_trimming_options.adapter_sequence1): |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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69 --adapter_sequence '$single_paired.adapter_trimming_options.adapter_sequence1' |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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70 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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71 |
5
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
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72 #if str($single_paired.single_paired_selector).startswith('paired'): |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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73 #if str($single_paired.adapter_trimming_options.adapter_sequence2): |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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74 --adapter_sequence_r2 '$single_paired.adapter_trimming_options.adapter_sequence2' |
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6c2351178f8d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 249269561e1d3a767371c154677e5dd59147f7d7
iuc
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75 #else |
6c2351178f8d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 249269561e1d3a767371c154677e5dd59147f7d7
iuc
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76 --detect_adapter_for_pe |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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77 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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78 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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79 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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80 |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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81 ## Global Trimming Options |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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82 |
988729b728f0
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iuc
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83 #if str($single_paired.global_trimming_options.trim_front1): |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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84 -f $single_paired.global_trimming_options.trim_front1 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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85 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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86 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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87 #if str($single_paired.global_trimming_options.trim_tail1): |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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88 -t $single_paired.global_trimming_options.trim_tail1 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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89 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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90 |
5
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
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91 #if str($single_paired.single_paired_selector).startswith('paired'): |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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92 #if str($single_paired.global_trimming_options.trim_front2): |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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93 -F $single_paired.global_trimming_options.trim_front2 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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94 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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95 #if str($single_paired.global_trimming_options.trim_tail2): |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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96 -T $single_paired.global_trimming_options.trim_tail2 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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97 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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98 #end if |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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99 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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100 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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101 ## Overrepresented sequence analysis |
988729b728f0
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102 |
988729b728f0
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103 $overrepresented_sequence_analysis.overrepresentation_analysis |
988729b728f0
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104 |
988729b728f0
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105 #if str($overrepresented_sequence_analysis.overrepresentation_sampling): |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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106 -P $overrepresented_sequence_analysis.overrepresentation_sampling |
988729b728f0
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107 #end if |
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108 |
988729b728f0
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109 |
1
f44e93b4529c
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110 ## Filter Options |
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111 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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112 ## Quality filtering options |
f44e93b4529c
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113 |
f44e93b4529c
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114 $filter_options.quality_filtering_options.disable_quality_filtering |
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115 |
f44e93b4529c
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116 #if str($filter_options.quality_filtering_options.qualified_quality_phred): |
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iuc
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117 -q $filter_options.quality_filtering_options.qualified_quality_phred |
f44e93b4529c
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iuc
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118 #end if |
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iuc
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119 #if str($filter_options.quality_filtering_options.unqualified_percent_limit): |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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120 -u $filter_options.quality_filtering_options.unqualified_percent_limit |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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121 #end if |
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iuc
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122 #if str($filter_options.quality_filtering_options.n_base_limit): |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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123 -n $filter_options.quality_filtering_options.n_base_limit |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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124 #end if |
f44e93b4529c
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iuc
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125 |
f44e93b4529c
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126 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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127 ## Length filtering options |
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128 |
f44e93b4529c
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iuc
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129 $filter_options.length_filtering_options.disable_length_filtering |
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130 |
f44e93b4529c
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iuc
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131 #if str($filter_options.length_filtering_options.length_required): |
f44e93b4529c
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iuc
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132 -l $filter_options.length_filtering_options.length_required |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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133 #end if |
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134 |
9
dbf9c561ef29
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
iuc
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135 #if str($filter_options.length_filtering_options.length_limit): |
dbf9c561ef29
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
iuc
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136 --length_limit $filter_options.length_filtering_options.length_limit |
dbf9c561ef29
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
iuc
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137 #end if |
dbf9c561ef29
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit a64eb7904f80e90a5faff04ea3fb797ee1e07d36"
iuc
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138 |
1
f44e93b4529c
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iuc
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139 ## Low complexity filtering options |
f44e93b4529c
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140 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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141 $filter_options.low_complexity_filter.enable_low_complexity_filter |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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142 |
f44e93b4529c
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iuc
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143 #if str($filter_options.low_complexity_filter.complexity_threshold): |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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144 -Y $filter_options.low_complexity_filter.complexity_threshold |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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145 #end if |
f44e93b4529c
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iuc
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146 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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147 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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148 ## Read Modification Options |
f44e93b4529c
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149 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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150 ## PolyG tail trimming, useful for NextSeq/NovaSeq data |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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151 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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152 #if $read_mod_options.polyg_tail_trimming.trimming_select in ['', '-g']: |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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153 #if str($read_mod_options.polyg_tail_trimming.poly_g_min_len): |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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154 --poly_g_min_len $read_mod_options.polyg_tail_trimming.poly_g_min_len |
f44e93b4529c
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iuc
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155 #end if |
f44e93b4529c
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iuc
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156 $read_mod_options.polyg_tail_trimming.trimming_select |
f44e93b4529c
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iuc
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157 #end if |
f44e93b4529c
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iuc
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158 |
f44e93b4529c
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iuc
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159 ## PolyX tail trimming |
f44e93b4529c
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iuc
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160 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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161 #if $read_mod_options.polyx_tail_trimming.polyx_trimming_select == '-x': |
f44e93b4529c
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iuc
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162 $read_mod_options.polyx_tail_trimming.polyx_trimming_select |
6
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
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163 #if str($read_mod_options.polyx_tail_trimming.poly_x_min_len): |
1
f44e93b4529c
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164 --poly_x_min_len $read_mod_options.polyx_tail_trimming.poly_x_min_len |
f44e93b4529c
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iuc
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165 #end if |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
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166 #end if |
f44e93b4529c
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iuc
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167 |
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168 ## UMI processing |
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169 |
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170 #if $read_mod_options.umi_processing.umi: |
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171 $read_mod_options.umi_processing.umi |
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172 #if str($read_mod_options.umi_processing.umi_loc): |
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173 --umi_loc '$read_mod_options.umi_processing.umi_loc' |
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174 #end if |
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175 #if str($read_mod_options.umi_processing.umi_len): |
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176 --umi_len $read_mod_options.umi_processing.umi_len |
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177 #end if |
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178 #if str($read_mod_options.umi_processing.umi_prefix): |
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179 --umi_prefix '$read_mod_options.umi_processing.umi_prefix' |
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180 #end if |
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181 #end if |
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182 |
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183 ## Per read cutting by quality options |
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184 |
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185 #if $read_mod_options.cutting_by_quality_options.cut_by_quality5 or $read_mod_options.cutting_by_quality_options.cut_by_quality3: |
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186 |
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187 $read_mod_options.cutting_by_quality_options.cut_by_quality5 |
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188 |
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189 $read_mod_options.cutting_by_quality_options.cut_by_quality3 |
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190 |
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191 #if str($read_mod_options.cutting_by_quality_options.cut_window_size): |
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192 -W $read_mod_options.cutting_by_quality_options.cut_window_size |
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193 #end if |
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194 #if str($read_mod_options.cutting_by_quality_options.cut_mean_quality): |
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195 -M $read_mod_options.cutting_by_quality_options.cut_mean_quality |
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196 #end if |
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197 #end if |
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198 |
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199 ## Base correction by overlap analysis options |
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200 |
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201 $read_mod_options.base_correction_options.correction |
0
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202 |
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203 && |
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204 |
4
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205 mv first${ext} '${out1}' |
5
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206 #if str($single_paired.single_paired_selector).startswith('paired'): |
0
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207 && |
4
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208 mv second${ext} '${out2}' |
0
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209 #end if |
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210 ]]></command> |
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211 <inputs> |
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212 |
0
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213 <conditional name="single_paired"> |
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214 <param name="single_paired_selector" type="select" label="Single-end or paired reads"> |
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215 <option value="single" selected="true">Single-end</option> |
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216 <option value="paired">Paired</option> |
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217 <option value="paired_collection">Paired Collection</option> |
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218 </param> |
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219 <when value="single"> |
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220 <expand macro="in" /> |
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221 <expand macro="adapter_trimming_options" /> |
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222 <expand macro="global_trimming_options" /> |
0
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223 </when> |
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224 <when value="paired"> |
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225 <expand macro="in" read_number="1" argument="-i"/> |
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226 <expand macro="in" read_number="2" argument="-I"/> |
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227 <expand macro="adapter_trimming_options"> |
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228 <expand macro="adapter_sequence" read_number="2"/> |
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229 </expand> |
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230 <expand macro="global_trimming_options_paired" /> |
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231 </when> |
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232 <when value="paired_collection"> |
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233 <param name="paired_input" type="data_collection" collection_type="paired" format="fastq,fastq.gz" label="Select paired collection(s)"/> |
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234 <expand macro="adapter_trimming_options"> |
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235 <expand macro="adapter_sequence" read_number="2"/> |
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236 </expand> |
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237 <expand macro="global_trimming_options_paired" /> |
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238 </when> |
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239 </conditional> |
1
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240 |
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241 <section name="overrepresented_sequence_analysis" title="Overrepresented Sequence Analysis" expanded="False"> |
0
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242 <param name="overrepresentation_analysis" argument="-p" type="boolean" truevalue="-p" falsevalue="" checked="false" label="Enable overrepresented analysis" help="Enable overrepresented sequence analysis."/> |
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243 <param name="overrepresentation_sampling" argument="-P" type="integer" optional="true" label="Overrepresentation sampling" help="One in (--overrepresentation_sampling) reads will be computed for overrepresentation analysis (1~10000), smaller is slower, default is 20."/> |
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244 </section> |
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245 |
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246 <!-- Filter Options --> |
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247 <section name="filter_options" title="Filter Options"> |
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248 <section name="quality_filtering_options" title="Quality filtering options" expanded="True"> |
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249 <param name="disable_quality_filtering" argument="-Q" type="boolean" truevalue="-Q" falsevalue="" checked="false" label="Disable quality filtering" help="Quality filtering is enabled by default. If this option is specified, quality filtering is disabled."/> |
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250 <param name="qualified_quality_phred" argument="-q" type="integer" optional="true" label="Qualified quality phred" help="The quality value that a base is qualified. Default 15 means phred quality >=Q15 is qualified."/> |
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251 <param name="unqualified_percent_limit" argument="-u" type="integer" optional="true" label="Unqualified percent limit" help="How many percents of bases are allowed to be unqualified (0~100). Default 40 means 40%."/> |
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252 <param name="n_base_limit" argument="-n" type="integer" optional="true" label="N base limit" help="If one read's number of N base is >n_base_limit, then this read/pair is discarded. Default is 5."/> |
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253 </section> |
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254 |
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255 <section name="length_filtering_options" title="Length filtering options" expanded="True"> |
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256 <param name="disable_length_filtering" argument="-L" type="boolean" truevalue="-L" falsevalue="" checked="false" label="Disable length filtering" help="Length filtering is enabled by default. If this option is specified, length filtering is disabled."/> |
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257 <param name="length_required" argument="-l" type="integer" optional="true" label="Length required" help="Reads shorter than this value will be discarded. Default is 15."/> |
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258 <param name="length_limit" argument="--length_limit" type="integer" optional="true" label="Maximum length" help="Reads longer than this value will be discarded. Default is 0 and means no limitation."/> |
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259 </section> |
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260 |
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261 <section name="low_complexity_filter" title="Low complexity filtering options" expanded="True"> |
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262 <param name="enable_low_complexity_filter" argument="-y" type="boolean" truevalue="-y" falsevalue="" checked="false" label="Enable low complexity filter" help="The complexity is defined as the percentage of base that is different from its next base, default is No"/> |
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263 <param name="complexity_threshold" argument="-Y" type="integer" optional="true" label="Complexity threshold" help="Threshold for low complexity filter (0~100). Default is 30, which means 30% complexity is required."/> |
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264 </section> |
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265 </section> |
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266 |
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267 <!-- Read Modification Options --> |
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268 <section name="read_mod_options" title="Read Modification Options"> |
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269 <conditional name="polyg_tail_trimming"> |
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270 <param name="trimming_select" type="select" label="PolyG tail trimming" help="This feature is enabled for NextSeq/NovaSeq data by default. NextSeq/NovaSeq data is detected by the machine ID in the FASTQ records."> |
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271 <option value="" selected="true">Automatic trimming for Illumina NextSeq/NovaSeq data</option> |
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272 <option value="-g">Force polyG tail trimming</option> |
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273 <option value="-G">Disable polyG tail trimming</option> |
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274 </param> |
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275 <when value="-g"> |
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276 <expand macro="poly_g_min_len" /> |
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277 </when> |
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278 <when value=""> |
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279 <expand macro="poly_g_min_len" /> |
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280 </when> |
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281 <when value="-G" /> |
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282 </conditional> |
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283 |
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284 <conditional name="polyx_tail_trimming"> |
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285 <param name="polyx_trimming_select" type="select" label="PolyX tail trimming" help="Similar to polyG tail trimming. When polyG tail trimming and polyX tail trimming are both enabled, fastp will perform polyG trimming first, then perform polyX trimming. Disabled by default."> |
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286 <option value="" selected="true">Disable polyX trimming</option> |
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287 <option value="-x">Enable polyX tail trimming</option> |
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288 </param> |
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289 <when value="-x"> |
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290 <param name="poly_x_min_len" argument="--poly_x_min_len" type="integer" optional="true" label="PolyX minimum length" |
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291 help="The minimum length to detect polyX in the read tail. 10 by default."/> |
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292 </when> |
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293 <when value="" /> |
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294 </conditional> |
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295 |
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296 <section name="umi_processing" title="UMI processing" expanded="True"> |
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297 <param name="umi" argument="-U" type="boolean" truevalue="-U" falsevalue="" checked="false" label="Enable unique molecular identifer" help="Enable unique molecular identifer (UMI) preprocessing."/> |
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298 <param name="umi_loc" argument="--umi_loc" type="text" optional="true" label="UMI location" help="Specify the location of UMI, can be (index1/index2/read1/read2/per_index/per_read, default is none."/> |
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299 <param name="umi_len" argument="--umi_len" type="integer" optional="true" label="UMI length" help="If the UMI is in read1/read2, its length should be provided."/> |
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300 <param name="umi_prefix" argument="--umi_prefix" type="text" optional="true" label="UMI prefix" help="If specified, an underline will be used to connect prefix and UMI (i.e. prefix=UMI, UMI=AATTCG, final=UMI_AATTCG). No prefix by default."/> |
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301 </section> |
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302 |
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303 <section name="cutting_by_quality_options" title="Per read cutting by quality options" expanded="True"> |
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304 <param name="cut_by_quality5" argument="-5" type="boolean" truevalue="-5" falsevalue="" checked="false" label="Cut by quality in front (5')" help="Enable per read cutting by quality in front (5'), default is disabled (WARNING: this will interfere deduplication for both PE/SE data)."/> |
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305 <param name="cut_by_quality3" argument="-3" type="boolean" truevalue="-3" falsevalue="" checked="false" label="Cut by quality in tail (3')" help="Enable per read cutting by quality in tail (3'), default is disabled (WARNING: this will interfere deduplication for SE data)."/> |
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306 <param name="cut_window_size" argument="-W" type="integer" optional="true" label="Cutting window size" help="The size of the sliding window for sliding window trimming, default is 4."/> |
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307 <param name="cut_mean_quality" argument="-M" type="integer" optional="true" label="Cutting mean quality" help="The bases in the sliding window with mean quality below cutting_quality will be cut, default is Q20."/> |
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308 </section> |
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309 |
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310 <section name="base_correction_options" title="Base correction by overlap analysis options" expanded="True"> |
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311 <param name="correction" argument="-c" type="boolean" truevalue="-c" falsevalue="" checked="false" label="Enable base correction" help="Enable base correction in overlapped regions (only for PE data), default is disabled."/> |
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312 </section> |
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313 </section> |
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314 |
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315 <section name="output_options" title="Output Options" expanded="False"> |
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316 <param name="report_html" type="boolean" truevalue="True" falsevalue="False" checked="True" label="Output HTML report" help="fastp provides a QC report for the data Before and After filtering within a single HTML page, which enables comparison of the quality statistics changed by the preprocessing step directly"/> |
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317 <param name="report_json" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Output JSON report" help="The JSON report contains all the data visualized in the HTML report. The format of the JSON report is manually optimized to be easily readable by humans and is compatible with MultiQC"/> |
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318 </section> |
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319 </inputs> |
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320 |
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321 <outputs> |
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322 <data name="out1" format_source="in1" label="${tool.name} on ${on_string}: Read 1 output"> |
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323 <filter>single_paired['single_paired_selector'] in ["single", "paired"]</filter> |
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324 </data> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6e59980ea50d84a849190460ae44c055f643355f
iuc
parents:
7
diff
changeset
|
325 <data name="out2" format_source="in2" label="${tool.name} on ${on_string}: Read 2 output"> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
326 <filter>single_paired['single_paired_selector'] == "paired"</filter> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
327 </data> |
8
1d8fe9bc4cb0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6e59980ea50d84a849190460ae44c055f643355f
iuc
parents:
7
diff
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328 <collection name="output_paired_coll" type="paired" format_source="paired_input['forward']" label="${tool.name} on ${on_string}: Paired-end output"> |
5
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
329 <filter>single_paired['single_paired_selector'] == "paired_collection"</filter> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
330 </collection> |
8
1d8fe9bc4cb0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6e59980ea50d84a849190460ae44c055f643355f
iuc
parents:
7
diff
changeset
|
331 <data name="report_html" format="html" from_work_dir="fastp.html" label="${tool.name} on ${on_string}: HTML report"> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
332 <filter>output_options['report_html'] is True</filter> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
333 </data> |
8
1d8fe9bc4cb0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6e59980ea50d84a849190460ae44c055f643355f
iuc
parents:
7
diff
changeset
|
334 <data name="report_json" format="json" from_work_dir="fastp.json" label="${tool.name} on ${on_string}: JSON report"> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
335 <filter>output_options['report_json'] is True</filter> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
336 </data> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
337 </outputs> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
338 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
339 <tests> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
340 <!-- Ensure default output works --> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
341 <test expect_num_outputs="2"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
342 <param name="in1" ftype="fastqsanger" value="R1.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
343 <param name="single_paired_selector" value="single"/> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
344 <output name="out1" ftype="fastqsanger" file="out1.fq"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
345 <output name="report_html"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
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346 <assert_contents> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
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347 <has_text text="fastp report"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
348 </assert_contents> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
349 </output> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
350 </test> |
5
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
351 <!-- Ensure paired collection works --> |
7
6c2351178f8d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 249269561e1d3a767371c154677e5dd59147f7d7
iuc
parents:
6
diff
changeset
|
352 <test expect_num_outputs="4"> |
5
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
353 <param name="single_paired_selector" value="paired_collection"/> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
354 <param name="paired_input"> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
355 <collection type="paired"> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
356 <element name="forward" value="bwa-mem-fastq1.fq" ftype="fastqsanger" /> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
357 <element name="reverse" value="bwa-mem-fastq2.fq" ftype="fastqsanger" /> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
358 </collection> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
359 </param> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
360 <output name="report_html"> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
361 <assert_contents> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
362 <has_text text="fastp report"/> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
363 </assert_contents> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
364 </output> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
365 <output_collection name="output_paired_coll" type="paired"> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
366 <element name="forward" value="out_bwa1.fq" ftype="fastqsanger"/> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
367 <element name="reverse" value="out_bwa2.fq" ftype="fastqsanger"/> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
368 </output_collection> |
3e2551a37201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit f3673fca1a180ada77fca93d871b3fd446f74498
iuc
parents:
4
diff
changeset
|
369 </test> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
370 <!-- Ensure custom adapter works --> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
371 <test expect_num_outputs="2"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
372 <param name="in1" ftype="fastq" value="R1.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
373 <param name="single_paired_selector" value="single"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
374 <param name="adapter_sequence1" value="ATCG"/> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
375 <output name="out1" ftype="fastq" file="out_a.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
376 </test> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
377 <!-- Ensure UMI processing works --> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
378 <test expect_num_outputs="2"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
379 <param name="in1" ftype="fastq" value="R1.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
380 <param name="single_paired_selector" value="single"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
381 <section name="umi_processing"> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
382 <param name="umi" value="true"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
383 <param name="umi_loc" value="read1"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
384 <param name="umi_len" value="8"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
385 </section> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
386 <output name="out1" ftype="fastq" file="out2.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
387 </test> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
388 <!-- Ensure UMI processing with different lengths works --> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
389 <test expect_num_outputs="2"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
390 <param name="in1" ftype="fastq" value="R1.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
391 <param name="single_paired_selector" value="single"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
392 <section name="umi_processing"> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
393 <param name="umi" value="true"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
394 <param name="umi_loc" value="read1"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
395 <param name="umi_len" value="12"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
396 </section> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
397 <output name="out1" ftype="fastq" file="out3.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
398 </test> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
399 <!-- Ensure paired-end fastq works --> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
400 <test expect_num_outputs="3"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
401 <param name="in1" ftype="fastq" value="bwa-mem-fastq1.fq"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
402 <param name="in2" ftype="fastq" value="bwa-mem-fastq2.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
403 <param name="single_paired_selector" value="paired"/> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
404 <output name="out1" ftype="fastq" file="out_bwa1.fq"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
405 <output name="out2" ftype="fastq" file="out_bwa2.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
406 </test> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
407 <!-- Ensure paired-end UMI processing of Read 1 works --> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
408 <test expect_num_outputs="3"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
409 <param name="in1" ftype="fastq" value="bwa-mem-fastq1.fq"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
410 <param name="in2" ftype="fastq" value="bwa-mem-fastq2.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
411 <param name="single_paired_selector" value="paired"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
412 <section name="umi_processing"> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
413 <param name="umi" value="true"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
414 <param name="umi_loc" value="read1"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
415 <param name="umi_len" value="8"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
416 </section> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
417 <output name="out1" ftype="fastq" file="out_bwa_umi_read1_1.fq"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
418 <output name="out2" ftype="fastq" file="out_bwa_umi_read1_2.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
419 </test> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
420 <!-- Ensure paired-end UMI processing of Read 2 works --> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
421 <test expect_num_outputs="3"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
422 <param name="in1" ftype="fastq" value="bwa-mem-fastq1.fq"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
423 <param name="in2" ftype="fastq" value="bwa-mem-fastq2.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
424 <param name="single_paired_selector" value="paired"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
425 <section name="umi_processing"> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
426 <param name="umi" value="true"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
427 <param name="umi_loc" value="read2"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
428 <param name="umi_len" value="8"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
429 </section> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
430 <output name="out1" ftype="fastq" file="out_bwa_umi_read2_1.fq"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
431 <output name="out2" ftype="fastq" file="out_bwa_umi_read2_2.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
432 </test> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
433 <!-- Ensure JSON report output works --> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
434 <test expect_num_outputs="2"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
435 <param name="in1" ftype="fastqsanger" value="R1.fq"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
436 <param name="single_paired_selector" value="single"/> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
437 <param name="report_html" value="False"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
438 <param name="report_json" value="True"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
439 <output name="out1" ftype="fastqsanger" file="out1.fq"/> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
440 <output name="report_json"> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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diff
changeset
|
441 <assert_contents> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
|
442 <has_text text="fastp report"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
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443 </assert_contents> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
|
444 </output> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
445 </test> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
446 <!-- Ensure polyG trimming works --> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
447 <test expect_num_outputs="2"> |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
448 <param name="in1" ftype="fastq.gz" value="R1.fq.gz"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
449 <param name="single_paired_selector" value="single"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
450 <param name="trimming_select" value="-g"/> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
451 <param name="poly_g_min_len" value="10"/> |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
452 <output name="out1" ftype="fastq.gz" decompress="True" file="out1.fq.gz"/> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
453 </test> |
6
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents:
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diff
changeset
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454 <!-- Ensure polyX trimming works --> |
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents:
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diff
changeset
|
455 <test expect_num_outputs="2"> |
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents:
5
diff
changeset
|
456 <param name="in1" ftype="fastq.gz" value="R1.fq.gz"/> |
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents:
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diff
changeset
|
457 <param name="single_paired_selector" value="single"/> |
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents:
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diff
changeset
|
458 <param name="trimming_select" value="-G"/> |
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents:
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diff
changeset
|
459 <param name="polyx_trimming_select" value="-x"/> |
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents:
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diff
changeset
|
460 <param name="poly_x_min_len" value="10"/> |
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
parents:
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diff
changeset
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461 <output name="out1" ftype="fastq.gz" decompress="True" file="out1.fq.gz"/> |
a935cbdf1c0e
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 72f3e06941d063dffce10741fcebea38a536ee15
iuc
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diff
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462 </test> |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
|
463 </tests> |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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changeset
|
464 <help><![CDATA[ |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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465 .. class:: infomark |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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466 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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changeset
|
467 **What it does** |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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468 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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changeset
|
469 fastp_ is a tool designed to provide fast all-in-one preprocessing for FASTQ files. This tool is developed in C++ with multithreading supported to afford high performance. |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
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470 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
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changeset
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471 *Features* |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
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472 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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473 1. Filter out bad reads (too low quality, too short, or too many N...) |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
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474 |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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changeset
|
475 2. Cut low quality bases for per read in its 5' and 3' by evaluating the mean quality from a sliding window (like Trimmomatic but faster) |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
|
476 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
|
477 3. Trim all reads in front and tail |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
|
478 |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
479 4. Cut adapters. Adapter sequences can be automatically detected, which means you don't have to input the adapter sequences to trim them. |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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changeset
|
480 |
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
481 5. Correct mismatched base pairs in overlapped regions of paired end reads, if one base is with high quality while the other is with ultra-low quality |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
|
482 |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
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diff
changeset
|
483 6. Trim polyG in 3' ends, which is commonly seen in NovaSeq/NextSeq data. Trim polyX in 3' ends to remove unwanted polyX tailing (i.e. polyA tailing for mRNA-Seq data) |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
diff
changeset
|
484 |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
iuc
parents:
0
diff
changeset
|
485 7. Preprocess unique molecular identifer (UMI) enabled data, shift UMI to sequence name |
0
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
iuc
parents:
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changeset
|
486 |
1
f44e93b4529c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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487 8. Report JSON format result for further interpreting |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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488 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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489 9. Visualize quality control and filtering results on a single HTML page (like FASTQC but faster and more informative) |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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490 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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491 10. Split the output to multiple files (0001.R1.gz, 0002.R1.gz...) to support parallel processing. Two modes can be used, limiting the total split file number, or limitting the lines of each split file (*Not enabled in this Galaxy tool*) |
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988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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492 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 6918a14442b7ebc56950917d668356feefaaaa28
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493 11. Support long reads (data from PacBio / Nanopore devices) |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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494 |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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495 ----- |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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496 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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497 **Inputs** |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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498 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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499 Single-end or Paired-end FASTQ or FASTQ.GZ reads |
988729b728f0
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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500 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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501 ----- |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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502 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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503 **Outputs** |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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504 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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505 * Processed reads |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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506 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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507 Optionally, under **Output Options** you can choose to output |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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508 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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509 * HTML report (default is Yes) |
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510 * JSON report (compatible with MultiQC) |
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511 |
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512 .. _fastp: https://github.com/OpenGene/fastp |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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513 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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514 ]]></help> |
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515 <citations> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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516 <citation type="doi">10.1101/274100</citation> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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517 </citations> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastp commit 2e41f5d3dd8201a5f8295ec30095c508250a8304
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518 </tool> |