annotate flash.xml @ 0:13e98e2709b8 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/flash commit 48012d1879395ecf1b4e6cd962f325c372164a33
author iuc
date Tue, 26 Sep 2017 16:42:09 -0400
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13e98e2709b8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/flash commit 48012d1879395ecf1b4e6cd962f325c372164a33
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1 <?xml version="1.0"?>
13e98e2709b8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/flash commit 48012d1879395ecf1b4e6cd962f325c372164a33
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2 <tool id="flash" name="FLASH" version="1.2.11">
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3 <description>adjust length of short reads</description>
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4 <requirements>
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5 <requirement type="package" version="1.2.11">flash</requirement>
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6 </requirements>
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7 <version_command>flash --version | head -n 1</version_command>
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8 <command detect_errors="aggressive">
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9 <![CDATA[
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10 flash --threads=\${GALAXY_SLOTS:-1}
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11 -m $min_overlap
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12 -M $max_overlap
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13 -x $max_mismatch_density
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14 $allow_outies
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15 '$forward' '$reverse'
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16 ]]>
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17 </command>
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18 <inputs>
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19 <param format="fastq" name="forward" type="data" label="Forward reads" />
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20 <param format="fastq" name="reverse" type="data" label="Reverse reads" />
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21 <param name="min_overlap" argument="--min-overlap" type="integer" optional="true" value="10" label="Minimum overlap" help="The minimum required overlap length between two reads to provide a confident overlap." />
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22 <param name="max_overlap" argument="--max-overlap" type="integer" optional="true" value="65" label="Maximum overlap" help="Maximum overlap length expected in approximately 90% of read pairs. Overlaps longer than the maximum overlap parameter are still considered as good overlaps, but the mismatch density is calculated over the first max_overlap bases in the overlapped region rather than the entire overlap." />
13e98e2709b8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/flash commit 48012d1879395ecf1b4e6cd962f325c372164a33
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23 <param name="max_mismatch_density" argument="--max-mismatch-density" type="float" optional="true" value="0.25" label="Maximum mismatch density" help="Maximum allowed ratio between the number of mismatched base pairs and the overlap length. Two reads will not be combined with a given overlap if that overlap results in a mismatched base density higher than this value." />
13e98e2709b8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/flash commit 48012d1879395ecf1b4e6cd962f325c372164a33
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24 <param name="allow_outies" argument="--allow-outies" type="boolean" truevalue="--allow-outies" falsevalue="" checked="false" label="Combine read pairs in both orientations" help="FLASH uses the same parameters when trying each orientation. If a read pair can be combined in either orientation, the better-fitting one will be chosen using the same scoring algorithm that FLASH normally uses." />
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25 </inputs>
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26 <outputs>
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27 <data format="fastq" name="merged_reads" from_work_dir="out.extendedFrags.fastq" label="${tool.name} on ${on_string}: Merged reads" />
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28 <data format="fastq" name="unmerged_reads_f" from_work_dir="out.notCombined_1.fastq" label="${tool.name} on ${on_string}: Unmerged forward reads" />
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29 <data format="fastq" name="unmerged_reads_r" from_work_dir="out.notCombined_2.fastq" label="${tool.name} on ${on_string}: Unmerged reverse reads" />
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30 <data format="tabular" name="hist" from_work_dir="out.hist" label="${tool.name} on ${on_string}: Unmerged reads tabular histogram" />
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31 <data format="txt" name="histogram" from_work_dir="out.histogram" label="${tool.name} on ${on_string}: Unmerged reads histogram" />
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32 <data format="tabular" name="hist_in" from_work_dir="out.hist.innie" label="${tool.name} on ${on_string}: Unmerged reads tabular histogram (in)" >
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33 <filter>allow_outies</filter>
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34 </data>
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35 <data format="tabular" name="hist_out" from_work_dir="out.hist.outie" label="${tool.name} on ${on_string}: Unmerged reads tabular histogram (out)" >
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36 <filter>allow_outies</filter>
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37 </data>
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38 <data format="txt" name="histogram_in" from_work_dir="out.histogram.innie" label="${tool.name} on ${on_string}: Unmerged reads histogram (in)" >
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39 <filter>allow_outies</filter>
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40 </data>
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41 <data format="txt" name="histogram_out" from_work_dir="out.histogram.outie" label="${tool.name} on ${on_string}: Unmerged reads histogram (out)" >
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42 <filter>allow_outies</filter>
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43 </data>
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44 </outputs>
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45 <tests>
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46 <test>
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47 <param name="forward" value="flash_forward_in1.fastq" />
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48 <param name="reverse" value="flash_reverse_in1.fastq" />
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49 <output name="merged_reads" file="flash_merged_out1.fastq" />
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50 <output name="unmerged_reads_f" file="flash_unmerged_f_out1.fastq" />
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51 <output name="unmerged_reads_r" file="flash_unmerged_r_out1.fastq" />
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52 <output name="hist" file="flash_hist_out1.tabular" />
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53 <output name="histogram" file="flash_hist_out1.txt" />
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54 </test>
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55 <test>
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56 <param name="forward" value="flash_forward_in2.fastq" />
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57 <param name="reverse" value="flash_reverse_in2.fastq" />
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58 <param name="allow_outies" value="true" />
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59 <output name="merged_reads" file="flash_merged_out2.fastq" />
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60 <output name="unmerged_reads_f" file="flash_unmerged_f_out2.fastq" />
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61 <output name="unmerged_reads_r" file="flash_unmerged_r_out2.fastq" />
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62 <output name="hist" file="flash_hist_out2.tabular" />
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63 <output name="histogram" file="flash_hist_out2.txt" />
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64 <output name="hist_in" file="flash_hist_in_out2.tabular" />
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65 <output name="histogram_in" file="flash_hist_in_out2.txt" />
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66 <output name="hist_out" file="flash_hist_out_out2.tabular" />
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67 <output name="histogram_out" file="flash_hist_out_out2.txt" />
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68 </test>
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69 </tests>
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70 <help>
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71 <![CDATA[
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72 FLASH (Fast Length Adjustment of SHort reads) is an accurate and fast tool
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73 to merge paired-end reads that were generated from DNA fragments whose
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74 lengths are shorter than twice the length of reads. Merged read pairs result
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75 in unpaired longer reads, which are generally more desired in genome
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76 assembly and genome analysis processes.
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77
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78 Briefly, the FLASH algorithm considers all possible overlaps at or above a
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79 minimum length between the reads in a pair and chooses the overlap that
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80 results in the lowest mismatch density (proportion of mismatched bases in
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81 the overlapped region). Ties between multiple overlaps are broken by
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82 considering quality scores at mismatch sites. When building the merged
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83 sequence, FLASH computes a consensus sequence in the overlapped region.
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84 ]]>
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85 </help>
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86 <citations>
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87 <citation type="doi">10.1093/bioinformatics/btr507</citation>
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88 </citations>
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89 </tool>