Mercurial > repos > iuc > genebed_maf_to_fasta

annotate interval_maf_to_merged_fasta.py @ 0:f24a9ff28d3c draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
author iuc
date Fri, 21 Aug 2020 15:10:13 -0400
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f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
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1 #!/usr/bin/env python
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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2 """
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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3 Reads an interval or gene BED and a MAF Source.
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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4 Produces a FASTA file containing the aligned intervals/gene sequences, based upon the provided coordinates
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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5
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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6 Alignment blocks are layered ontop of each other based upon score.
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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7
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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8 usage: %prog maf_file [options]
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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9 -d, --dbkey=d: Database key, ie hg17
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10 -c, --chromCol=c: Column of Chr
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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11 -s, --startCol=s: Column of Start
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12 -e, --endCol=e: Column of End
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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13 -S, --strandCol=S: Column of Strand
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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14 -G, --geneBED: Input is a Gene BED file, process and join exons as one region
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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15 -t, --mafSourceType=t: Type of MAF source to use
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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16 -m, --mafSource=m: Path of source MAF file, if not using cached version
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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17 -I, --mafIndex=I: Path of precomputed source MAF file index, if not using cached version
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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18 -i, --interval_file=i: Input interval file
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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19 -o, --output_file=o: Output MAF file
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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20 -p, --species=p: Species to include in output
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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21 -O, --overwrite_with_gaps=O: Overwrite bases found in a lower-scoring block with gaps interior to the sequence for a species.
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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22 -z, --mafIndexFileDir=z: Directory of local maf_index.loc file
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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23
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24 usage: %prog dbkey_of_BED comma_separated_list_of_additional_dbkeys_to_extract comma_separated_list_of_indexed_maf_files input_gene_bed_file output_fasta_file cached|user GALAXY_DATA_INDEX_DIR
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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25 """
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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26 # Dan Blankenberg
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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27 from __future__ import print_function
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28
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29 import sys
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30
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31 import bx.intervals.io
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32 from bx.cookbook import doc_optparse
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33 from galaxy.tools.util import maf_utilities
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34
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36 def stop_err(msg):
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37 sys.exit(msg)
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38
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39
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40 def __main__():
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41 # Parse Command Line
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42 options, args = doc_optparse.parse(__doc__)
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43 mincols = 0
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44 strand_col = -1
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45
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46 if options.dbkey:
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47 primary_species = options.dbkey
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48 else:
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49 primary_species = None
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50 if primary_species in [None, "?", "None"]:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
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51 stop_err("You must specify a proper build in order to extract alignments. You can specify your genome build by clicking on the pencil icon associated with your interval file.")
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52
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53 include_primary = True
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54 secondary_species = maf_utilities.parse_species_option(options.species)
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55 if secondary_species:
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56 species = list(secondary_species) # make copy of species list
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57 if primary_species in secondary_species:
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58 secondary_species.remove(primary_species)
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59 else:
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60 include_primary = False
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61 else:
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62 species = None
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63
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64 if options.interval_file:
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65 interval_file = options.interval_file
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66 else:
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67 stop_err("Input interval file has not been specified.")
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68
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69 if options.output_file:
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70 output_file = options.output_file
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71 else:
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72 stop_err("Output file has not been specified.")
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73
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74 if not options.geneBED:
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75 if options.chromCol:
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76 chr_col = int(options.chromCol) - 1
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77 else:
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78 stop_err("Chromosome column not set, click the pencil icon in the history item to set the metadata attributes.")
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79
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diff changeset
80 if options.startCol:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
81 start_col = int(options.startCol) - 1
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
82 else:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
83 stop_err("Start column not set, click the pencil icon in the history item to set the metadata attributes.")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
84
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
85 if options.endCol:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
86 end_col = int(options.endCol) - 1
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
87 else:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
88 stop_err("End column not set, click the pencil icon in the history item to set the metadata attributes.")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
89
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
90 if options.strandCol:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
91 strand_col = int(options.strandCol) - 1
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
92
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
93 mafIndexFile = "%s/maf_indexes.loc" % options.mafIndexFileDir
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
94
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
95 overwrite_with_gaps = True
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
96 if options.overwrite_with_gaps and options.overwrite_with_gaps.lower() == 'false':
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
97 overwrite_with_gaps = False
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
98
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
99 # Finish parsing command line
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
100
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
101 # get index for mafs based on type
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
102 index = index_filename = None
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
103 # using specified uid for locally cached
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
104 if options.mafSourceType.lower() in ["cached"]:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
105 index = maf_utilities.maf_index_by_uid(options.mafSource, mafIndexFile)
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
106 if index is None:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
107 stop_err("The MAF source specified (%s) appears to be invalid." % (options.mafSource))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
108 elif options.mafSourceType.lower() in ["user"]:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
109 # index maf for use here, need to remove index_file when finished
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
110 index, index_filename = maf_utilities.open_or_build_maf_index(options.mafSource, options.mafIndex, species=[primary_species])
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
111 if index is None:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
112 stop_err("Your MAF file appears to be malformed.")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
113 else:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
114 stop_err("Invalid MAF source type specified.")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
115
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
116 # open output file
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
117 output = open(output_file, "w")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
118
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
119 if options.geneBED:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
120 region_enumerator = maf_utilities.line_enumerator(open(interval_file, "r").readlines())
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
121 else:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
122 region_enumerator = enumerate(bx.intervals.io.NiceReaderWrapper(
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
123 open(interval_file, 'r'), chrom_col=chr_col, start_col=start_col,
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
124 end_col=end_col, strand_col=strand_col, fix_strand=True,
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
125 return_header=False, return_comments=False))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
126
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
127 # Step through intervals
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
128 regions_extracted = 0
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
129 line_count = 0
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
130 for line_count, line in region_enumerator:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
131 try:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
132 if options.geneBED: # Process as Gene BED
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
133 try:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
134 starts, ends, fields = maf_utilities.get_starts_ends_fields_from_gene_bed(line)
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
135 # create spliced alignment object
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
136 alignment = maf_utilities.get_spliced_region_alignment(
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
137 index, primary_species, fields[0], starts, ends,
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
138 strand='+', species=species, mincols=mincols,
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
139 overwrite_with_gaps=overwrite_with_gaps)
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
140 except Exception as e:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
141 print(e)
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
142 try:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
143 primary_name = secondary_name = fields[3]
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
144 alignment_strand = fields[5]
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
145 except Exception as e:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
146 print("Error loading exon positions from input line %i: %s" % (line_count, e))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
147 continue
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
148 else: # Process as standard intervals
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
149 try:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
150 # create spliced alignment object
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
151 alignment = maf_utilities.get_region_alignment(
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
152 index, primary_species, line.chrom, line.start,
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
153 line.end, strand='+', species=species, mincols=mincols,
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
154 overwrite_with_gaps=overwrite_with_gaps)
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
155 primary_name = "%s(%s):%s-%s" % (line.chrom, line.strand, line.start, line.end)
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
156 secondary_name = ""
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
157 alignment_strand = line.strand
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
158 except Exception as e:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
159 print("Error loading region positions from input line %i: %s" % (line_count, e.__dict__))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
160 continue
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
161
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
162 # Write alignment to output file
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
163 # Output primary species first, if requested
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
164 if include_primary:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
165 output.write(">%s.%s\n" % (primary_species, primary_name))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
166 if alignment_strand == "-":
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
167 output.write(alignment.get_sequence_reverse_complement(primary_species))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
168 else:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
169 output.write(alignment.get_sequence(primary_species))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
170 output.write("\n")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
171 # Output all remainging species
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
172 for spec in secondary_species or alignment.get_species_names(skip=primary_species):
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
173 if secondary_name:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
174 output.write(">%s.%s\n" % (spec, secondary_name))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
175 else:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
176 output.write(">%s\n" % (spec))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
177 if alignment_strand == "-":
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
178 output.write(alignment.get_sequence_reverse_complement(spec))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
179 else:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
180 output.write(alignment.get_sequence(spec))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
181 output.write("\n")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
182
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
183 output.write("\n")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
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184 regions_extracted += 1
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
185 except Exception as e:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
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186 print("Unexpected error from input line %i: %s\n%s" % (line_count, e, line))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
187 raise
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
188
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
189 # close output file
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
190 output.close()
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
191
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
192 # remove index file if created during run
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
193 maf_utilities.remove_temp_index_file(index_filename)
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
194
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
195 # Print message about success for user
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
196 if regions_extracted > 0:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
197 print("%i regions were processed successfully." % (regions_extracted))
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
198 else:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
199 print("No regions were processed successfully.")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
200 if line_count > 0 and options.geneBED:
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
201 print("This tool requires your input file to conform to the 12 column BED standard.")
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
202
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
203
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
204 if __name__ == "__main__":
f24a9ff28d3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genebed_maf_to_fasta/ commit 17e2194066ca843f6b2391a9632ea9de67d39351"
iuc
parents:
diff changeset
205 __main__()