Mercurial > repos > iuc > genetrack
annotate genetrack_util.py @ 0:25cd59a002d9 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
author | iuc |
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date | Tue, 22 Dec 2015 17:03:27 -0500 |
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children | df7ac50ade5d |
rev | line source |
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0
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
|
1 import bisect |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
|
2 import math |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
|
3 import numpy |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
|
4 import re |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
|
5 import subprocess |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
|
6 import sys |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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7 import tempfile |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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8 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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9 GFF_EXT = 'gff' |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
|
10 SCIDX_EXT = 'scidx' |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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11 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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12 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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13 def noop(data): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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14 return data |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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15 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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16 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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17 def zeropad_to_numeric(data): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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18 return re.sub(r'chr0(\d)', r'chr\1', data) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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19 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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20 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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21 def numeric_to_zeropad(data): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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22 return re.sub(r'chr(\d([^\d]|$))', r'chr0\1', data) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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23 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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24 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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25 FORMATS = ['zeropad', 'numeric'] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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26 IN_CONVERT = {'zeropad': zeropad_to_numeric, 'numeric': noop} |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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27 OUT_CONVERT = {'zeropad': numeric_to_zeropad, 'numeric': noop} |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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28 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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29 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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30 def conversion_functions(in_fmt, out_fmt): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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31 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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32 Returns the proper list of functions to apply to perform a conversion |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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33 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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34 return [IN_CONVERT[in_fmt], OUT_CONVERT[out_fmt]] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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35 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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36 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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37 def convert_data(data, in_fmt, out_fmt): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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38 for fn in conversion_functions(in_fmt, out_fmt): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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39 data = fn(data) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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40 return data |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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41 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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42 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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43 class ChromosomeManager(object): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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44 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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45 Manages a CSV reader of an index file to only load one chrom at a time |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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46 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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47 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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48 def __init__(self, reader): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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49 self.done = False |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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50 self.reader = reader |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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51 self.processed_chromosomes = [] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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52 self.current_index = 0 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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53 self.next_valid() |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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54 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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changeset
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55 def next(self): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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56 self.line = self.reader.next() |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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57 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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58 def is_valid(self, line): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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changeset
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59 if len(line) not in [4, 5, 9]: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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60 return False |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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61 try: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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62 [int(i) for i in line[1:]] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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63 self.format = SCIDX_EXT |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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64 return True |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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65 except ValueError: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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66 try: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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67 if len(line) < 6: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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68 return False |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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69 [int(line[4]), int(line[5])] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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70 self.format = GFF_EXT |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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71 return True |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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72 except ValueError: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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73 return False |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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changeset
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74 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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75 def next_valid(self): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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76 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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changeset
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77 Advance to the next valid line in the reader |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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78 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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79 self.line = self.reader.next() |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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80 s = 0 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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81 while not self.is_valid(self.line): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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82 self.line = self.reader.next() |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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83 s += 1 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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84 if s > 0: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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85 # Skip initial line(s) of file |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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86 pass |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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87 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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diff
changeset
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88 def parse_line(self, line): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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changeset
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89 if self.format == SCIDX_EXT: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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90 return [int(line[1]), int(line[2]), int(line[3])] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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91 else: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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92 return [int(line[3]), line[6], line[5]] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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diff
changeset
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93 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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diff
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94 def chromosome_name(self): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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95 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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96 Return the name of the chromosome about to be loaded |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
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97 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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98 return self.line[0] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
diff
changeset
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99 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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100 def load_chromosome(self, collect_data=True): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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101 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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102 Load the current chromosome into an array and return it |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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103 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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104 cname = self.chromosome_name() |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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105 if cname in self.processed_chromosomes: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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106 stop_err('File is not grouped by chromosome') |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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107 self.data = [] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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108 while self.line[0] == cname: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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109 if collect_data: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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110 read = self.parse_line(self.line) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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111 if read[0] < self.current_index: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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112 msg = 'Reads in chromosome %s are not sorted by index. (At index %d)' % (cname, self.current_index) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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113 stop_err(msg) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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114 self.current_index = read[0] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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115 self.add_read(read) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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116 try: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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117 self.next() |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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118 except StopIteration: |
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iuc
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119 self.done = True |
25cd59a002d9
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120 break |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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121 self.processed_chromosomes.append(cname) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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122 self.current_index = 0 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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123 data = self.data |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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124 # Don't retain reference anymore to save memory |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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125 del self.data |
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iuc
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126 return data |
25cd59a002d9
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127 |
25cd59a002d9
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128 def add_read(self, read): |
25cd59a002d9
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129 if self.format == SCIDX_EXT: |
25cd59a002d9
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iuc
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130 self.data.append(read) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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131 else: |
25cd59a002d9
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iuc
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132 index, strand, value = read |
25cd59a002d9
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iuc
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133 if value == '' or value == '.': |
25cd59a002d9
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iuc
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134 value = 1 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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135 else: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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136 value = int(value) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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137 if not self.data: |
25cd59a002d9
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iuc
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138 self.data.append([index, 0, 0]) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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139 current_read = self.data[-1] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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140 if self.data[-1][0] == index: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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141 current_read = self.data[-1] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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142 elif self.data[-1][0] < index: |
25cd59a002d9
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iuc
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143 self.data.append([index, 0, 0]) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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144 current_read = self.data[-1] |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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145 else: |
25cd59a002d9
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iuc
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146 msg = 'Reads in chromosome %s are not sorted by index. (At index %d)' % (self.chromosome_name(), index) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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147 stop_err(msg) |
25cd59a002d9
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148 if strand == '+': |
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iuc
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149 current_read[1] += value |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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150 elif strand == '-': |
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iuc
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151 current_read[2] += value |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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152 else: |
25cd59a002d9
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153 msg = 'Strand "%s" at chromosome "%s" index %d is not valid.' % (strand, self.chromosome_name(), index) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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154 stop_err(msg) |
25cd59a002d9
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155 |
25cd59a002d9
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156 def skip_chromosome(self): |
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157 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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158 Skip the current chromosome, discarding data |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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159 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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160 self.load_chromosome(collect_data=False) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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161 |
25cd59a002d9
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iuc
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162 |
25cd59a002d9
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iuc
parents:
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163 class Peak(object): |
25cd59a002d9
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iuc
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164 def __init__(self, index, pos_width, neg_width): |
25cd59a002d9
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165 self.index = index |
25cd59a002d9
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iuc
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166 self.start = index - neg_width |
25cd59a002d9
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iuc
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167 self.end = index + pos_width |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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168 self.value = 0 |
25cd59a002d9
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iuc
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169 self.deleted = False |
25cd59a002d9
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170 self.safe = False |
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iuc
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171 |
25cd59a002d9
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172 def __repr__(self): |
25cd59a002d9
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173 return '[%d] %d' % (self.index, self.value) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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174 |
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175 |
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176 def gff_row(cname, start, end, score, source, type='.', strand='.', phase='.', attrs={}): |
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177 return (cname, source, type, start, end, score, strand, phase, gff_attrs(attrs)) |
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178 |
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179 |
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180 def gff_attrs(d): |
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181 if not d: |
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182 return '.' |
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183 return ';'.join('%s=%s' % item for item in d.items()) |
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184 |
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185 |
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186 def stop_err(msg): |
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187 sys.stderr.write(msg) |
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188 sys.exit(1) |
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189 |
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190 |
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191 def is_int(i): |
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192 try: |
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193 int(i) |
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194 return True |
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195 except ValueError: |
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196 return False |
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197 |
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198 |
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199 def make_keys(data): |
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200 return [read[0] for read in data] |
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201 |
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202 |
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203 def make_peak_keys(peaks): |
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204 return [peak.index for peak in peaks] |
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205 |
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206 |
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207 def get_window(data, start, end, keys): |
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208 """ |
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209 Returns all reads from the data set with index between the two indexes |
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210 """ |
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211 start_index = bisect.bisect_left(keys, start) |
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212 end_index = bisect.bisect_right(keys, end) |
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213 return data[start_index:end_index] |
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214 |
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215 |
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216 def get_index(value, keys): |
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217 """ |
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218 Returns the index of the value in the keys using bisect |
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219 """ |
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220 return bisect.bisect_left(keys, value) |
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221 |
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222 |
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223 def get_range(data): |
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224 lo = min([item[0] for item in data]) |
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225 hi = max([item[0] for item in data]) |
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226 return lo, hi |
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227 |
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228 |
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229 def get_chunks(lo, hi, size, overlap=500): |
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230 """ |
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231 Divides a range into chunks of maximum size size. Returns a list of |
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232 2-tuples (slice_range, process_range), each a 2-tuple (start, end). |
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233 process_range has zero overlap and should be given to process_chromosome |
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234 as-is, and slice_range is overlapped and should be used to slice the |
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235 data (using get_window) to be given to process_chromosome. |
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236 """ |
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237 chunks = [] |
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238 for start_index in range(lo, hi, size): |
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239 process_start = start_index |
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240 # Don't go over upper bound |
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241 process_end = min(start_index + size, hi) |
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242 # Don't go under lower bound |
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243 slice_start = max(process_start - overlap, lo) |
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244 # Don't go over upper bound |
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245 slice_end = min(process_end + overlap, hi) |
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246 chunks.append(((slice_start, slice_end), (process_start, process_end))) |
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247 return chunks |
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248 |
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249 |
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250 def allocate_array(data, width): |
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251 """ |
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252 Allocates a new array with the dimensions required to fit all reads in |
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253 the argument. The new array is totally empty. Returns the array and the |
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254 shift (number to add to a read index to get the position in the array it |
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255 should be at). |
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256 """ |
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257 lo, hi = get_range(data) |
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258 rng = hi - lo |
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259 shift = width - lo |
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260 return numpy.zeros(rng+width*2, numpy.float), shift |
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261 |
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262 |
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263 def normal_array(width, sigma, normalize=True): |
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264 """ |
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265 Returns an array of the normal distribution of the specified width |
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266 """ |
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267 sigma2 = float(sigma)**2 |
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268 |
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269 def normal_func(x): |
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270 return math.exp(-x * x / (2 * sigma2)) |
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271 |
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272 # width is the half of the distribution |
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273 values = map(normal_func, range(-width, width)) |
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274 values = numpy.array(values, numpy.float) |
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275 # normalization |
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276 if normalize: |
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277 values = 1.0/math.sqrt(2 * numpy.pi * sigma2) * values |
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278 return values |
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279 |
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280 |
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281 def call_peaks(array, shift, data, keys, direction, down_width, up_width, exclusion): |
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282 peaks = [] |
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283 |
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284 def find_peaks(): |
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285 # Go through the array and call each peak |
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286 results = (array > numpy.roll(array, 1)) & (array > numpy.roll(array, -1)) |
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287 indexes = numpy.where(results) |
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288 for index in indexes[0]: |
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289 pos = down_width or exclusion // 2 |
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290 neg = up_width or exclusion // 2 |
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291 # Reverse strand |
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292 if direction == 2: |
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293 # Swap positive and negative widths |
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294 pos, neg = neg, pos |
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295 peaks.append(Peak(int(index)-shift, pos, neg)) |
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296 find_peaks() |
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297 |
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298 def calculate_reads(): |
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299 # Calculate the number of reads in each peak |
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300 for peak in peaks: |
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301 reads = get_window(data, peak.start, peak.end, keys) |
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302 peak.value = sum([read[direction] for read in reads]) |
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303 # Flat list of indexes with frequency |
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304 indexes = [r for read in reads for r in [read[0]] * read[direction]] |
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305 peak.stddev = numpy.std(indexes) |
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306 calculate_reads() |
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307 |
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308 def perform_exclusion(): |
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309 # Process the exclusion zone |
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310 peak_keys = make_peak_keys(peaks) |
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311 peaks_by_value = peaks[:] |
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312 peaks_by_value.sort(key=lambda peak: -peak.value) |
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313 for peak in peaks_by_value: |
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314 peak.safe = True |
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315 window = get_window(peaks, |
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316 peak.index-exclusion//2, |
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317 peak.index+exclusion//2, |
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318 peak_keys) |
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319 for excluded in window: |
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320 if excluded.safe: |
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321 continue |
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322 i = get_index(excluded.index, peak_keys) |
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323 del peak_keys[i] |
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324 del peaks[i] |
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325 perform_exclusion() |
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326 return peaks |
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327 |
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328 |
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329 def process_chromosome(cname, data, writer, process_bounds, width, sigma, down_width, up_width, exclusion, filter): |
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330 """ |
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331 Process a chromosome. Takes the chromosome name, list of reads, a CSV |
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332 writer to write processes results to, the bounds (2-tuple) to write |
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333 results in, and options. |
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334 """ |
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335 if not data: |
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336 return |
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337 keys = make_keys(data) |
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338 # Create the arrays that hold the sum of the normals |
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339 forward_array, forward_shift = allocate_array(data, width) |
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340 reverse_array, reverse_shift = allocate_array(data, width) |
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341 normal = normal_array(width, sigma) |
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342 |
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343 def populate_array(): |
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344 # Add each read's normal to the array |
25cd59a002d9
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345 for read in data: |
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346 index, forward, reverse = read |
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347 # Add the normals to the appropriate regions |
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348 if forward: |
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349 forward_array[index+forward_shift-width:index+forward_shift+width] += normal * forward |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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350 if reverse: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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351 reverse_array[index+reverse_shift-width:index+reverse_shift+width] += normal * reverse |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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352 populate_array() |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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353 forward_peaks = call_peaks(forward_array, forward_shift, data, keys, 1, down_width, up_width, exclusion) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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354 reverse_peaks = call_peaks(reverse_array, reverse_shift, data, keys, 2, down_width, up_width, exclusion) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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355 # Convert chromosome name in preparation for writing output |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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356 cname = convert_data(cname, 'zeropad', 'numeric') |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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357 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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358 def write(cname, strand, peak): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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359 start = max(peak.start, 1) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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360 end = peak.end |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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361 value = peak.value |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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362 stddev = peak.stddev |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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363 if value > filter: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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364 # This version of genetrack outputs only gff files. |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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365 writer.writerow(gff_row(cname=cname, |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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366 source='genetrack', |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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367 start=start, |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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368 end=end, |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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369 score=value, |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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370 strand=strand, |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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371 attrs={'stddev': stddev})) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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372 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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373 for peak in forward_peaks: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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374 if process_bounds[0] < peak.index < process_bounds[1]: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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375 write(cname, '+', peak) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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376 for peak in reverse_peaks: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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377 if process_bounds[0] < peak.index < process_bounds[1]: |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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378 write(cname, '-', peak) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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379 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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380 |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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381 def sort_chromosome_reads_by_index(input_path): |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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382 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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383 Return a gff file with chromosome reads sorted by index. |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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384 """ |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
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385 # Will this sort produce different results across platforms? |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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386 output_path = tempfile.NamedTemporaryFile(delete=False).name |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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387 command = 'sort -k 1,1 -k 4,4n "%s" > "%s"' % (input_path, output_path) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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388 p = subprocess.Popen(command, shell=True) |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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389 p.wait() |
25cd59a002d9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/genetrack commit e96df94dba60050fa28aaf55b5bb095717a5f260
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390 return output_path |