Mercurial > repos > iuc > gubbins
annotate gubbins.xml @ 0:637ec5d5368c draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
author | iuc |
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date | Fri, 23 Jun 2017 08:29:43 -0400 |
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children | 96e6283e4745 |
rev | line source |
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0
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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1 <tool id="gubbins" name="Gubbins" version="0.1.0"> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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2 <description>Recombination detection in Bacteria</description> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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3 <requirements> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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4 <requirement type="package" version="2.2.1">gubbins</requirement> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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5 </requirements> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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6 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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7 <version_command>run_gubbins.py --version</version_command> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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8 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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9 <command detect_errors="exit_code"><![CDATA[ |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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10 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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11 ln -s '$alignment_file' foo.aln && |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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12 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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13 run_gubbins.py |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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14 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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15 --threads \${GALAXY_SLOTS:-1} |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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16 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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17 #if $adv.iters |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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18 -i '$adv.iters' |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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19 #end if |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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20 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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21 #if $adv.conv_meth |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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changeset
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22 -z '$adv.conv_meth' |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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23 #end if |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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24 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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25 #if $adv.outgroup |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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26 -o '$adv.outgroup' |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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27 #end if |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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28 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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29 #if $really_adv.tree_builder |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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30 -t '$really_adv.tree_builder' |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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31 #end if |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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32 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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33 #if $really_adv.min_snps |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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34 -m '$really_adv.min_snps' |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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35 #end if |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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36 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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37 #if $really_adv.filter_percentage |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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38 -f '$really_adv.filter_percentage' |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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39 #end if |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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40 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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41 #if $really_adv.min_window_size |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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42 -a '$really_adv.min_window_size' |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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43 #end if |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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44 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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45 #if $really_adv.max_window_size |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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46 -b '$really_adv.max_window_size' |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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47 #end if |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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48 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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49 $really_adv.remove_duplicates |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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50 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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51 foo.aln |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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52 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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53 ]]></command> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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54 <inputs> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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55 <!-- Just the data set.. --> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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56 <param type="data" name="alignment_file" format="fasta" label="Whole genome alignment file" help="Whole genome alignment file in fasta format"/> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
parents:
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57 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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58 <!-- output file picker --> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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59 <param type="select" name="outfiles" multiple="true" display="checkboxes" label="Select the required output files" help="Default selections are the Final Tree in Newick format, the Recombination Predictions in gff3 format and the Summary of SNP Distribution"> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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60 <option value="ftree" selected="true">Final Tree in newick format</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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61 <option value="gff" selected="true">Recombination Predictions in gff3 format</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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62 <option value="vcf" selected="true">Summary of SNP Distribution in vcf format</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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63 <option value="recomb_embl">Recombination Predictions in embl format</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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64 <option value="fpoly">Filtered Polymorphic Sites in fasta format</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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65 <option value="ppoly">Filtered Polymorphic Sites in phylip format</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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66 <option value="stats">Per Branch Statistics in csv format</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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67 <option value="baseb">Base Branch Reconstruction in embl format</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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68 </param> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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69 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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70 <!-- A semi advanced section, really shouldn't have to change anything. --> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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71 <section name="adv" title="Advanced options" expanded="True"> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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72 <param name="iters" type="integer" label="Iterations" value="5" help="Maximum No. of iterations, default is 5" /> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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73 <param name="conv_meth" type="select" label="Model Conversion Options" help="Criteria to use to know when to halt iterations"> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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74 <option value="weighted_robinson_foulds" selected="True">Weighted Robinson Foulds</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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75 <option value="robinson_foulds">Robinson Foulds</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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76 <option value="recombination">Recombination</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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77 </param> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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78 <param name="outgroup" type="text" label="Outgroup" value="" help="Outgroup name for rerooting. A list of comma separated names can be used if they form a clade."/> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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79 </section> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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80 |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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81 <!-- a really advanced section, play with this at your peril! --> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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82 <section name="really_adv" title="Really advanced options - change these if you really know what you are doing." expanded="false"> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
iuc
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83 <param name="tree_builder" type="select" label="Tree builder" help="Application to use for tree building, default RAxML"> |
637ec5d5368c
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84 <option value="raxml" selected="true">RAxML</option> |
637ec5d5368c
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85 <option value="fasttree">FastTree</option> |
637ec5d5368c
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86 <option value="hybrid">Hybrid</option> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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87 </param> |
637ec5d5368c
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88 <param name="min_snps" type="integer" label="Minimum SNPS" value="3" help="Min SNPs to identify a recombination block, default is 3" /> |
637ec5d5368c
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89 <param name="filter_percentage" type="integer" label="Filter Percentage" value="25" help="Filter out taxa with more than this percentage of gaps, default is 25" /> |
637ec5d5368c
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90 <param name="min_window_size" type="integer" label="Minimum Window Size" value="100" help="Minimum window size, default 100" /> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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91 <param name="max_window_size" type="integer" label="Maximum Window Size" value="10000" help="Maximum window size, default 10000" /> |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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92 <param name="remove_duplicates" type="boolean" label="Remove identical sequences" falsevalue="" truevalue="-d" /> |
637ec5d5368c
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93 </section> |
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94 |
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95 </inputs> |
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96 |
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97 <outputs> |
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98 <data format="txt" name="final_tree" label="${tool.name} on ${on_string} Final Tree" from_work_dir="foo.final_tree.tre" > |
637ec5d5368c
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99 <filter>outfiles and 'ftree' in outfiles</filter> |
637ec5d5368c
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100 </data> |
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101 <data format="gff3" name="recomb_pred_gff" label="${tool.name} on ${on_string} Recombinations Prediction gff" from_work_dir="foo.recombination_predictions.gff" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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102 <filter>outfiles and 'gff' in outfiles</filter> |
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103 </data> |
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104 <data format="embl" name="recomb_pred_embl" label="${tool.name} on ${on_string} Recombinations Prediction embl" from_work_dir="foo.recombination_predictions.embl" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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105 <filter>outfiles and 'recomb_embl' in outfiles</filter> |
637ec5d5368c
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106 </data> |
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107 <data format="fasta" name="filt_polymorph_fna" label="${tool.name} on ${on_string} Filtered Polymorphic Sites fasta" from_work_dir="foo.filtered_polymorphic_sites.fasta" > |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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108 <filter>outfiles and 'fpoly' in outfiles</filter> |
637ec5d5368c
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109 </data> |
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110 <data format="phylip" name="filt_polymorph_phy" label="${tool.name} on ${on_string} Filtered Polymorphic Sites phylip" from_work_dir="foo.filtered_polymorphic_sites.phylip" > |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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111 <filter>outfiles and 'ppoly' in outfiles</filter> |
637ec5d5368c
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112 </data> |
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113 <data format="csv" name="per_b_stat_csv" label="${tool.name} on ${on_string} Per Branch Statistics csv" from_work_dir="foo.per_branch_statistics.csv" > |
637ec5d5368c
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114 <filter>outfiles and 'stats' in outfiles</filter> |
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115 </data> |
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116 <data format="vcf" name="sum_snp_vcf" label="${tool.name} on ${on_string} Summary of SNP Distribution vcf" from_work_dir="foo.summary_of_snp_distribution.vcf" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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117 <filter>outfiles and 'vcf' in outfiles</filter> |
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118 </data> |
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119 <data format="embl" name="base_branch_embl" label="${tool.name} on ${on_string} Branch Base Reconstruction embl" from_work_dir="foo.branch_base_reconstruction.embl" > |
637ec5d5368c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gubbins commit 98be6edc2544513347149b50e0cfb530811f890a
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120 <filter>outfiles and 'baseb' in outfiles</filter> |
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121 </data> |
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122 </outputs> |
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123 |
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124 |
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125 <tests> |
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126 <test> |
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127 <param name="alignment_file" value="multiple_recombinations.aln" ftype="fasta" /> |
637ec5d5368c
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128 <output name="recomb_pred_gff"> |
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129 <assert_contents> |
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130 <has_text text="##gff-version 3" /> |
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131 </assert_contents> |
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132 </output> |
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133 <output name="sum_snp_vcf"> |
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134 <assert_contents> |
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135 <has_text text="##fileformat=VCFv4.2" /> |
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136 </assert_contents> |
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137 </output> |
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138 </test> |
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139 |
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140 <test> |
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141 <param name="alignment_file" value="multiple_recombinations.aln" ftype="fasta" /> |
637ec5d5368c
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142 <param name="iters" value="3"/> |
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143 <output name="recomb_pred_gff"> |
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144 <assert_contents> |
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145 <has_text text="##gff-version 3" /> |
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146 </assert_contents> |
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147 </output> |
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148 <output name="sum_snp_vcf"> |
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149 <assert_contents> |
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150 <has_text text="##fileformat=VCFv4.2" /> |
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151 </assert_contents> |
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152 </output> |
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153 </test> |
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154 |
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155 <test> |
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156 <param name="alignment_file" value="multiple_recombinations.aln" ftype="fasta" /> |
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157 <param name="conv_meth" value="recombination" /> |
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158 <output name="recomb_pred_gff"> |
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159 <assert_contents> |
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160 <has_text text="##gff-version 3" /> |
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161 </assert_contents> |
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162 </output> |
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163 <output name="sum_snp_vcf"> |
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164 <assert_contents> |
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165 <has_text text="##fileformat=VCFv4.2" /> |
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166 </assert_contents> |
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167 </output> |
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168 </test> |
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169 |
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170 <test> |
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171 <param name="alignment_file" value="multiple_recombinations.aln" ftype="fasta" /> |
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172 <param name="conv_meth" value="recombination" /> |
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173 <param name="outfiles" value="gff,vcf,ftree,recomb_embl,fpoly,ppoly,stats,baseb"/> |
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174 <output name="recomb_pred_gff"> |
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175 <assert_contents> |
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176 <has_text text="##gff-version 3" /> |
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177 </assert_contents> |
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178 </output> |
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179 <output name="sum_snp_vcf"> |
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180 <assert_contents> |
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181 <has_text text="##fileformat=VCFv4.2" /> |
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182 </assert_contents> |
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183 </output> |
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184 <output name="filt_polymorph_fna"> |
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185 <assert_contents> |
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186 <has_text text=">sequence_1" /> |
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187 </assert_contents> |
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188 </output> |
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189 <output name="recomb_pred_embl"> |
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190 <assert_contents> |
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191 <has_text text="FT" /> |
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192 </assert_contents> |
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193 </output> |
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194 <output name="filt_polymorph_phy"> |
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195 <assert_contents> |
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196 <has_text text="sequence_1" /> |
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197 </assert_contents> |
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198 </output> |
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199 <output name="per_b_stat_csv"> |
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200 <assert_contents> |
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201 <has_text text="Node" /> |
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202 </assert_contents> |
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203 </output> |
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204 <output name="base_branch_embl"> |
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205 <assert_contents> |
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206 <has_text text="FT" /> |
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207 </assert_contents> |
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208 </output> |
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209 <output name="final_tree"> |
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210 <assert_contents> |
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211 <has_text text="((sequence_" /> |
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212 </assert_contents> |
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213 </output> |
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214 </test> |
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215 </tests> |
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216 |
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217 |
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218 <help><![CDATA[ |
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219 **Gubbins** |
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220 |
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221 Since the introduction of high-throughput, second-generation DNA sequencing technologies, there has been an enormous increase in the size of datasets being used for estimating bacterial population phylodynamics. Although many phylogenetic techniques are scalable to hundreds of bacterial genomes, methods which have been used for mitigating the effect of mechanisms of horizontal sequence transfer on phylogenetic reconstructions cannot cope with these new datasets. Gubbins (Genealogies Unbiased By recomBinations In Nucleotide Sequences) is an algorithm that iteratively identifies loci containing elevated densities of base substitutions while concurrently constructing a phylogeny based on the putative point mutations outside of these regions. Simulations demonstrate the algorithm generates highly accurate reconstructions under realistic models of short-term bacterial evolution, and can be run in only a few hours on alignments of hundreds of bacterial genome sequences. |
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222 |
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223 **Running Gubbins** |
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224 |
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225 To run Gubbins with default settings: |
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226 |
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227 Supply a "fasta" genome alignment file and press execute. |
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228 |
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229 **Other options** |
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230 |
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231 **Advanced** |
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232 |
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233 *Iterations*, (-i) |
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234 |
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235 The maximum number of iterations to perform; the algorithm will stop earlier than this if it converges on the same tree in two successive iterations. Default is 5. |
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236 |
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237 *Converge_method* (-z) |
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238 |
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239 Criteria to use to know when to halt iterations [weighted_robinson_foulds|robinson_foulds|recombination]. Default is weighted_robinson_foulds. |
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240 |
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241 *Outgroup*, (-o) |
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242 |
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243 The name of a sequence in the alignment on which to root the tree |
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244 |
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245 **Really Advanced** |
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246 |
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247 These options are here for completeness and you shouldn't need to change them from their defaults. You really need to know what you are doing before you use these. |
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248 |
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249 *Tree builder*, (-t) |
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250 |
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251 The algorithm to use in the construction of phylogenies in the analysis; can be ‘raxml’, to use RAxML, ‘fasttree’, to use Fasttree, or ‘hybrid’, to use Fasttree for the first iteration and RAxML in all subsequent iterations. Default is raxml |
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252 |
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253 Filter Percentage, (-f) |
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254 |
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255 Filter out taxa with more than this percentage of missing data. Default is 25% |
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256 |
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257 *Minimum snps*, (-m) |
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258 |
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259 The minimum number of base substitutions required to identify a recombination. Default is 3. |
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260 |
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261 |
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262 And others... |
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263 |
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264 |
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265 |
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266 **Output files** |
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267 |
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268 * Recombination predictions in EMBL tab file format. |
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269 |
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270 |
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271 * Recombination predictions in GFF3 format |
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272 |
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273 |
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274 * Base substitution reconstruction in EMBL tab format. |
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275 |
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276 |
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277 * VCF file summarising the distribution of SNPs |
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278 |
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279 |
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280 * Per branch reporting of the base substitutions inside and outside recombinations events. |
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281 |
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282 |
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283 * FASTA format alignment of filtered polymorphic sites used to generate the phylogeny in the final iteration. |
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284 |
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285 |
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286 * Phylip format alignment of filtered polymorphic sites used to generate the phylogeny in the final iteration. |
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287 |
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288 |
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289 * Final phylogenetic tree in newick format. |
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290 |
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291 ]]></help> |
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292 |
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293 |
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294 <citations> |
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295 <citation type="doi">doi:10.1093/nar/gku1196</citation> |
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296 </citations> |
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297 |
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298 </tool> |