annotate macros.xml @ 0:62479bdcc059 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
author iuc
date Tue, 12 May 2015 15:04:26 -0400
parents
children b5066aa77fea
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
0
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1 <?xml version="1.0"?>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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2 <macros>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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3 <xml name="requirements">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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4 <requirements>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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5 <requirement type="package" version="3.1b1">hmmer3_1b1</requirement>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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6 <yield/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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7 </requirements>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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8 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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9 <token name="@WRAPPER_VERSION@">0.1</token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
10 <xml name="stdio">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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11 <stdio>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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12 <!-- Anything other than zero is an error -->
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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13 <exit_code range="1:"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
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14 <exit_code range=":-1"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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15 <!-- In case the return code has not been set propery check stderr too -->
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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16 <regex match="Error:"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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17 <regex match="Exception:"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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18 </stdio>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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19 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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20 <token name="@THRESHOLDS@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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21 -E $E
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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22 --domE $domE
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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23
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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24 #if $T:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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25 -T $T
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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26 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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27
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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28 #if $domT:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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29 --domT $domT
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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30 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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31
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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32 #if $incE:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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33 --incE $incE
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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34 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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35
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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36 #if $incT:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
37 --incT $incT
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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38 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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39
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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40 #if $incdomE:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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41 --incdomE $incdomE
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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42 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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43
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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44 #if $incdomT:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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45 --incdomT $incdomT
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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46 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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47 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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48 <xml name="thresholds_xml">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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49 <!-- Options controlling reporting thresholds -->
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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50 <param name="E" label="report sequences &lt;= this E-Value threshold in output" help="(-E)" value="10.0" type="float" min="0"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
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51 <param name="domE" label="report domains &lt;= this E-Value threshold in output" help="(--domE)" value="10.0" type="float" min="0"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
52 <param name="T" label="report sequences &gt;= this score threshold in output" help="(-T)" type="float" optional="True"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
53 <param name="domT" label="report domains &gt;= this score threshold in output" help="(--domT)" type="float" optional="True"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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54 <!-- Options controlling inclusion (significance) thresholds -->
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
55 <param name="incE" label="consider sequences &lt;= this E-Value threshold as significant" help="(--incE)" type="float" optional="True"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
56 <param name="incdomE" label="consider domains &lt;= this E-Value threshold as significant" help="(--incdomE)" type="float" optional="True"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
57 <param name="incT" label="consider sequences &gt;= this score threshold as significant" help="(--incT)" type="float" optional="True"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
58 <param name="incdomT" label="consider domains &gt;= this score threshold as significant" help="(--incdomT)" type="float" optional="True"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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59 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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60 <token name="@THRESHOLDS_NODOM@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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61 -E $E
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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62
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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63 #if $T:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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64 -T $T
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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65 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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66
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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67 #if $incE:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
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68 --incE $incE
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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69 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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70
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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71 #if $incT:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
72 --incT $incT
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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73 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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74 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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75 <xml name="thresholds_nodom">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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76 <!-- Options controlling reporting thresholds -->
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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77 <param name="E" label="report sequences &lt;= this E-Value threshold in output" help="(-E)" value="10.0" type="float" min="0"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
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78 <param name="T" label="report sequences &gt;= this score threshold in output" help="(-T)" type="float" optional="True"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
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79 <!-- Options controlling inclusion (significance) thresholds -->
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
80 <param name="incE" label="consider sequences &lt;= this E-Value threshold as significant" help="(--incE)" type="float" optional="True"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
81 <param name="incT" label="consider sequences &gt;= this score threshold as significant" help="(--incT)" type="float" optional="True"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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82 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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83 <token name="@ACCEL_HEUR@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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84 $max
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
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85 --F1 $F1
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
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86 --F2 $F2
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
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87 --F3 $F3
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
88 $nobias
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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89
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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90 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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91 <xml name="accel_heur_xml">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
92 <!-- Options controlling acceleration heuristics -->
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
93 <param name="max" type="boolean" truevalue="--max" label="Turn all heuristic filters off (less speed, more power)" help="(--max)" falsevalue=""/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
94 <param name="F1" type="float" label="Stage 1 (MSV) threshold: promote hits w/ P &lt;= F1" help="(--F1)" value="0.02"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
95 <param name="F2" type="float" label="Stage 2 (Vit) threshold: promote hits w/ P &lt;= F2" help="(--F2)" value="1e-3"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
96 <param name="F3" type="float" label="Stage 3 (Fwd) threshold: promote hits w/ P &lt;= F3" help="(--F3)" value="1e-5"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
97 <param name="nobias" type="boolean" truevalue="--nobias" label="Turn off composition bias filter" help="(--nobias)" falsevalue=""/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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98 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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99 <token name="@EVAL_CALIB@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
100 --EmL $EmL
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
101 --EmN $EmN
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
102 --EvL $EvL
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
103 --EvN $EvN
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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104 --EfL $EfL
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
105 --EfN $EfN
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
106 --Eft $Eft
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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107 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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108 <xml name="eval_calib_xml">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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109 <!-- Control of E-value calibration -->
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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110 <param name="EmL" type="integer" value="200" min="1" help="(--EmL)" label="Length of sequences for MSV Gumbel mu fit"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
111 <param name="EmN" type="integer" value="200" min="1" help="(--EmN)" label="Number of sequences for MSV Gumbel mu fit"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
112 <param name="EvL" type="integer" value="200" min="1" help="(--EvL)" label="Length of sequences for Viterbi Gumbel mu fit"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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113 <param name="EvN" type="integer" value="200" min="1" help="(--EvN)" label="Number of sequences for Viterbi Gumbel mu fit"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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114 <param name="EfL" type="integer" value="100" min="1" help="(--EfL)" label="Length of sequences for Forward exp tail tau fit"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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115 <param name="EfN" type="integer" value="200" min="1" help="(--EfN)" label="Number of sequences for Forward exp tail tau fit"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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116 <param name="Eft" type="float" value="0.04" min="0" max="1" help="(--Eft)" label="tail mass for Forward exponential tail tau fit"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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117 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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118 <token name="@OFORMAT_WITH_OPTS_NOPFAM@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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119 #if 'tblout' in str($oformat):
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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120 --tblout $tblout
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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121 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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122
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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123 #if 'domtblout' in str($oformat):
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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124 --domtblout $domtblout
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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125 #end if
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126
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127 $acc $noali $notextw
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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128 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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129 <xml name="oformat_with_opts_nopfam">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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130 <!-- Options directing output -->
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131 <param name="oformat" multiple="True" display="checkboxes" label="Output Formats" type="select">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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132 <option value="tblout" selected="true">Table of per-sequence hits (--tblout)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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133 <option value="domtblout" selected="true">Table of per-domain hits (--domtblout)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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134 </param>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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135 <param name="acc" type="boolean" truevalue="--acc" falsevalue="" label="Prefer accessions over names in output" help="(--acc)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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136 <param name="noali" type="boolean" truevalue="--noali" falsevalue="" label="Don't output alignments, so output is smaller" help="(--noali)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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diff changeset
137 <param name="notextw" type="boolean" truevalue="--notextw" falsevalue="" label="Unlimited ASCII text output line width" help="(--notextw)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
138 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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139 <token name="@OFORMAT_WITH_OPTS@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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140 #if 'tblout' in str($oformat):
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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141 --tblout $tblout
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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142 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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143
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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144 #if 'domtblout' in str($oformat):
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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145 --domtblout $domtblout
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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146 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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147
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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148 #if 'pfamtblout' in str($oformat):
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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149 --pfamtblout $pfamtblout
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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150 #end if
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151
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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152 $acc $noali $notextw
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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153 </token>
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154 <xml name="oformat_with_opts">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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155 <!-- Options directing output -->
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156 <param name="oformat" multiple="True" display="checkboxes" label="Output Formats" type="select">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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157 <option value="tblout" selected="true">Table of per-sequence hits (--tblout)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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158 <option value="domtblout" selected="true">Table of per-domain hits (--domtblout)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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159 <option value="pfamtblout" selected="true">Table of hits and domains in Pfam format (--pfamtblout)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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160 </param>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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161 <param name="acc" type="boolean" truevalue="--acc" falsevalue="" label="Prefer accessions over names in output" help="(--acc)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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162 <param name="noali" type="boolean" truevalue="--noali" falsevalue="" label="Don't output alignments, so output is smaller" help="(--noali)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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163 <param name="notextw" type="boolean" truevalue="--notextw" falsevalue="" label="Unlimited ASCII text output line width" help="(--notextw)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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164 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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165 <xml name="oformat_test">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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166 <param name="notextw" value="True" />
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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167 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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168 <!-- TODO: tblout will match 'pfamtblout,dfamtblout' -->
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169 <token name="@OFORMAT_WITH_OPTS_N@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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170 #if 'tblout' in str($oformat):
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171 --tblout $tblout
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172 #end if
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173
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174 #if 'dfamtblout' in str($oformat):
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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175 --dfamtblout $dfamtblout
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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176 #end if
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177
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178 #if 'aliscoresout' in str($oformat):
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179 --aliscoresout $aliscoresout
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180 #end if
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181
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182 $acc $noali $notextw
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183 </token>
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184 <xml name="oformat_with_opts_n">
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185 <!-- Options directing output -->
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186 <param name="oformat" multiple="True" display="checkboxes" label="Output Formats" type="select">
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187 <option value="tblout" selected="true">Table of hits (--tblout)</option>
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188 <option value="dfamtblout" selected="true">Table of hits in Dfam format (--dfamtblout)</option>
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189 <option value="aliscoresout">Scores for each position in each alignment to file (--aliscoresout)</option>
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190 </param>
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191 <param name="acc" type="boolean" truevalue="--acc" falsevalue="" label="Prefer accessions over names in output" help="(--acc)"/>
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192 <param name="noali" type="boolean" truevalue="--noali" falsevalue="" label="Don't output alignments, so output is smaller" help="(--noali)"/>
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193 <param name="notextw" type="boolean" truevalue="--notextw" falsevalue="" label="Unlimited ASCII text output line width" help="(--notextw)"/>
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194 </xml>
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195 <token name="@HSSI@">
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196 #if $hssi.hssi_select == "singlemx":
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197 --popen $hssi.popen
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198 --pextend $hssi.pextend
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199 #end if
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200 </token>
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201 <xml name="hssi">
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202 <!-- Handling single sequence inputs -->
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203 <conditional name="hssi">
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204 <param name="hssi_select" type="select" label="Options for handling single sequence inputs">
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205 <option value="false" selected="true">Disable</option>
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206 <option value="singlemx">Use substitution score matrix for single-sequence inputs</option>
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207 </param>
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208 <when value="singlemx">
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209 <param name="popen" type="float" value="0.02" label="Gap open probability" help="(--popen)" min="0.0" max="0.5"/>
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210 <param name="pextend" type="float" value="0.4" label="Gap extend probability" help="(--pextend)" min="0.0" max="1.0"/>
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211 </when>
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212 <when value="false">
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213 </when>
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214 <!-- -mx <s> : substitution score matrix (built-in matrices, with -singlemx)-->
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215 <!-- -mxfile <f> : read substitution score matrix from file <f> (with -singlemx)-->
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216 </conditional>
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217 </xml>
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218 <token name="@CPU@">
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219 --cpu \${GALAXY_SLOTS:-2}
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220 </token>
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221 <token name="@SEED@">
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222 --seed $seed
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223 </token>
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224 <xml name="seed">
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225 <param name="seed" label="RNG seed, 0 generates a random seed" value="42" type="integer" help="(--seed)" min="0"/>
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226 </xml>
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227 <xml name="seed_test">
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228 <param name="seed" value="4" />
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229 </xml>
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230 <token name="@ADV_OPTS@">
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231 $nonull2
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232
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233 #if $Z:
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234 -Z $Z
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235 #end if
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236
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237 #if $domZ:
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238 --domZ $domZ
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239 #end if
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240 </token>
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241 <xml name="adv_opts">
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242 <!-- Other options -->
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243 <param name="nonull2" type="boolean" truevalue="--nonull2" label="Turn off biased composition score corrections" help="(--nonull2)" falsevalue=""/>
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244 <param name="Z" type="integer" label="# of comparisons done for E-value calculation" help="(-Z)" optional="True"/>
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245 <param name="domZ" type="integer" label="# of significant sequences, for domain E-value calculation" help="(--domZ)" optional="True"/>
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246 </xml>
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247 <token name="@FORMAT_SELECTOR@">
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248 $input_format_select
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249 </token>
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250 <xml name="format_selector">
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251 <param name="input_format_select" type="select" label="Format of sequence and model">
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252 <option value="--amino">Protein</option>
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253 <option value="--dna">DNA</option>
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254 <option value="--rna">RNA</option>
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255 </param>
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256 </xml>
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257 <xml name="format_selector_noprot">
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258 <param name="input_format_select" type="select" label="Format of sequence and model">
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259 <option value="--dna">DNA</option>
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260 <option value="--rna">RNA</option>
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261 </param>
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262 </xml>
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263 <token name="@ARSWS@">
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264 $arsws.arsws_select
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265
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266 #if $arsws.arsws_select == "--wblosum":
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267 --wid $arsws.wid
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268 #end if
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269 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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270 <xml name="arsws">
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271 <!-- Alternative relative sequence weighting strategies -->
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272 <conditional name="arsws">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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273 <param name="arsws_select" type="select" label="Alternative relative sequence weighting strategies">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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274 <option value="--wpb" selected="true">Henikoff position-based weights (--wpb)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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275 <option value="--wgsc">Gerstein/Sonnhammer/Chothia tree weights (--wgsc)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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276 <option value="--wblosum">Henikoff simple filter weights (--wblosum)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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277 <option value="--wnone">don't do any relative weighting; set all to 1 (--wnnoe)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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278 <option value="--wgiven">use weights as given in MSA file (--wgiven)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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279 </param>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
280 <when value="--wpb">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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281 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
282 <when value="--wgsc">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
283 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
284 <when value="--wblosum">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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285 <param name="wid" label="Set identity cutoff" value="0.62" type="float" help="(--wid)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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286 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
287 <when value="--wnone">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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288 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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289 <when value="--wgiven">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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290 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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291 </conditional>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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292 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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293 <token name="@AEEWS@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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294 #if $aeews.aeews_select != "":
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iuc
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295 --$aeews.aeews_select
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iuc
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296 #if $aeews.aeews_select == "eent":
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iuc
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297 --eset $aeews.eset
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iuc
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diff changeset
298 --ere $aeews.ere
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iuc
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299 --esigma $aeews.esigma
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300 #elif $aeews.aeews_select == "eclust":
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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301 --eset $aeews.eset
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iuc
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302 --eid $aeews.eid
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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303 #end if
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304 #end if
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iuc
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305 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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306 <xml name="aeews">
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iuc
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307 <!-- Alternative effective sequence weighting strategies -->
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iuc
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diff changeset
308 <conditional name="aeews">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
309 <param name="aeews_select" type="select" label="Alternative effective sequence weighting strategies">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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310 <option value="">Disabled</option>
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iuc
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311 <option value="eent">Adjust eff seq # to achieve relative entropy target (--eent)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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312 <option value="eclust">Eff seq # is the # of single linkage clusters (--eclust)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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313 <option value="enone">No effective seq # weighting: just use nseq (--enone)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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314 </param>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
315 <when value="">
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iuc
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316 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
317 <when value="eent">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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318 <param name="eset" type="float" value="0" label="set eff seq # for all models" help="(--eset)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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319 <param name="ere" type="float" value="0" label="set minimum rel entropy/position" help="(--ere)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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320 <param name="esigma" type="float" value="45" label="set sigma param" help="(--esigma)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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321 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
322 <when value="eclust">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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323 <param name="eset" type="float" value="0" label="set eff seq # for all models" help="(--eset)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
324 <param name="eid" type="float" value="0.62" label="set fractional identity cutoff" min="0" max="1" help="(--eid)"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
325 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
326 <when value="enone">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
327 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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328 </conditional>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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329 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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330 <token name="@CUT@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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331 $cut_ga
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332 $cut_nc
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iuc
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333 $cut_tc
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334 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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335 <xml name="cut">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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336 <param name="cut_ga" type="boolean" truevalue="--cut_ga" label="use profile's GA gathering cutoffs to set all thresholding" help="(--cut_ga)" falsevalue=""/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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337 <param name="cut_nc" type="boolean" truevalue="--cut_nc" label="use profile's NC gathering cutoffs to set all thresholding" help="(--cut_nc)" falsevalue=""/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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338 <param name="cut_tc" type="boolean" truevalue="--cut_tc" label="use profile's TC gathering cutoffs to set all thresholding" help="(--cut_tc)" falsevalue=""/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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339 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
340 <token name="@MCSS@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
341 --$mcs.model_construction_strategy_select
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iuc
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342
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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343 #if $mcs.model_construction_strategy_select == "fast":
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344 --symfrac $mcs.symfrac
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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345 #end if
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346
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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347 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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348 <xml name="mcss">
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iuc
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349 <!-- Alternative model construction strategies -->
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iuc
parents:
diff changeset
350 <conditional name="mcs">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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351 <param name="model_construction_strategy_select" type="select" label="Model Construction Strategy">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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352 <option value="fast" selected="true">Assign columns with &gt;= symfrac residues as consensus (--fast)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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353 <option value="hand">Manual construction (requires reference annotation) (--hand)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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354 </param>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
355 <when value="fast">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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356 <param name="symfrac" value="0.5" type="float" label="Sets sym fraction controlling --fast construction"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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357 </when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
358 <when value="hand"></when>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
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359 </conditional>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
360 <param name="fragthresh" label="Fraction of alignment length, under which sequences are excluded" help="HMMER infers fragments if the sequence length L is less than or equal to a fraction x times the alignment length in columns (--fragthresh)" value="0.5" optional="True" type="float" />
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
361
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
362 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
363 <token name="@PRIOR@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
364 $aps_select
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
365 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
366 <xml name="prior">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
367 <param name="aps_select" type="select" label="Alternative Prior Strategies">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
368 <option value="" selected="true">Unspecified</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
369 <option value="--pnone">Don't use any prior; parameters are frequencies (--pnone)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
370 <option value="--plaplace">Use a Laplace +1 prior (--plaplace)</option>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
371 </param>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
372 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
373 <xml name="citation">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
374 <citations>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
375 <citation type="doi">10.1093/nar/gkr367</citation>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
376 </citations>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
377 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
378 <token name="@LENGTHS@">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
379 #if $w_beta:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
380 --w_beta $w_beta
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
381 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
382
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
383 #if $w_length:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
384 --w_length $w_length
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
385 #end if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
386
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
387 </token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
388 <xml name="lengths">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
389 <param name="w_beta" label="Tail mass at which window length is determined"
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
390 help="(--w_beta)" optional="True" type="float"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
391 <param name="w_length" label="Window Length"
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
392 help="(--w_length)" optional="True" type="integer" />
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
393 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
394 <xml name="input_hmm">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
395 <param name="hmmfile" type="data" label="HMM model" format="hmmer2,hmmer3"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
396 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
397 <xml name="input_msa">
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
398 <param name="msafile" type="data" label="MSA File" format="stockholm"/>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
399 </xml>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
400
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
401
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
402 <token name="@ACCEL_HEUR_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
403 Acceleration Heuristicts (--F1, --F2, --F3)
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
404 -------------------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
405
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
406 **MSV filter**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
407
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
408 The sequence is aligned to the profile using a specialized model that
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
409 allows multiple high-scoring local ungapped segments to match. The
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
410 optimal alignment score (Viterbi score) is calculated under this multi-
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
411 segment model, hence the term MSV, for “multi-segment Viterbi”. This is
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
412 HMMER’s main speed heuristic. The MSV score is comparable to BLAST’s sum
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
413 score (optimal sum of ungapped alignment segments). Roughly speaking,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
414 MSV is comparable to skipping the heuristic word hit and hit extension
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
415 steps of the BLAST acceleration algorithm.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
416
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
417 The MSV filter is very, very fast. In addition to avoiding indel
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
418 calculations in the dynamic programming table, it uses reduced precision
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
419 scores scaled to 8-bit integers, enabling acceleration via 16-way
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
420 parallel SIMD vector instructions.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
421
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
422 The MSV score is a true log-odds likelihood ratio, so it obeys
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
423 conjectures about the expected score distribution (Eddy, 2008) that
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
424 allow immediate and accurate calculation of the statistical significance
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
425 (P- value) of the MSV bit score.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
426
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
427 By default, comparisons with a P-value of ≤ 0.02 pass this filter,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
428 meaning that about 2% of nonhomol- ogous sequences are expected to pass.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
429 You can use the --F1 option to change this threshold. For example, --F1
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
430 <0.05> would pass 5% of the comparisons, making a search more sensitive
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
431 but slower. Setting the threshold to ≥ 1.0 (--F1 99 for example) assures
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
432 that all comparisons will pass. Shutting off the MSV filter may be
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
433 worthwhile if you want to make sure you don’t miss comparisons that have
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
434 a lot of scattered insertions and deletions. Alternatively, the --max
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
435 option causes the MSV filter step (and all other filter steps) to be
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
436 bypassed.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
437
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
438 The MSV bit score is calculated as a log-odds score using the null model
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
439 for comparison. No correction for a biased composition or repetitive
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
440 sequence is done at this stage. For comparisons involving biased
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
441 sequences and/or profiles, more than 2% of comparisons will pass the MSV
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
442 filter. At the end of search output, there is a line like:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
443
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
444 Passed MSV filter: 107917 (0.020272); expected 106468.8 (0.02)
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
445
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
446 which tells you how many and what fraction of comparisons passed the MSV
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
447 filter, versus how many (and what fraction) were expected.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
448
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
449 **Viterbi filter**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
450
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
451 The sequence is now aligned to the profile using a fast Viterbi algorithm for
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
452 optimal gapped alignment.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
453
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
454 This Viterbi implementation is specialized for speed. It is implemented in
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
455 8-way parallel SIMD vector instructions, using reduced precision scores that
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
456 have been scaled to 16-bit integers. Only one row of the dynamic programming
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
457 matrix is stored, so the routine only recovers the score, not the optimal
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
458 alignment itself. The reduced representation has limited range; local alignment
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
459 scores will not underflow, but high scoring comparisons can overflow and return
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
460 infinity, in which case they automatically pass the filter.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
461
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
462 The final Viterbi filter bit score is then computed using the appropriate null
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
463 model log likelihood (by default the biased composition filter model score, or
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
464 if the biased filter is off, just the null model score). If the P-value of this
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
465 score passes the Viterbi filter threshold, the sequence passes on to the next
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
466 step of the pipeline.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
467
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
468 The --F2 <x> option controls the P-value threshold for passing the Viterbi
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
469 filter score. The default is 0.001. The --max option bypasses all filters in
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
470 the pipeline. At the end of a search output, you will see a line like:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
471
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
472 Passed Vit filter: 2207 (0.00443803); expected 497.3 (0.001)
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
473
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
474 which tells you how many and what fraction of comparisons passed the Viterbi
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
475 filter, versus how many were expected.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
476
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
477 **Forward filter/parser**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
478
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
479 The sequence is now aligned to the profile using the full Forward algorithm,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
480 which calculates the likelihood of the target sequence given the profile,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
481 summed over the ensemble of all possible alignments.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
482
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
483 This is a specialized time- and memory-efficient Forward implementation called
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
484 the “Forward parser”. It is implemented in 4-way parallel SIMD vector
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
485 instructions, in full precision (32-bit floating point). It stores just enough
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
486 information that, in combination with the results of the Backward parser
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
487 (below), posterior probabilities of start and stop points of alignments
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
488 (domains) can be calculated in the domain definition step (below), although the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
489 detailed alignments themselves cannot be.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
490
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
491 The Forward filter bit score is calculated by correcting this score using the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
492 appropriate null model log likelihood (by default the biased composition filter
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
493 model score, or if the biased filter is off, just the null model score). If the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
494 P-value of this bit score passes the Forward filter threshold, the sequence
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
495 passes on to the next step of the pipeline.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
496
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
497 The bias filter score has no further effect in the pipeline. It is only used in
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
498 filter stages. It has no effect on final reported bit scores or P-values.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
499 Biased composition compensation for final bit scores is done by a more complex
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
500 domain-specific algorithm, described below.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
501
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
502 The --F3 <x> option controls the P-value threshold for passing the Forward
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
503 filter score. The default is 1e-5. The --max option bypasses all filters in the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
504 pipeline. At the end of a search output, you will see a line like:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
505
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
506 Passed Fwd filter: 1076 (0.00216371); expected 5.0 (1e-05)
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
507
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
508 which tells you how many and what fraction of comparisons passed the Forward
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
509 filter, versus how many were expected.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
510
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
511 **Bias Filter Options**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
512
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
513 The --max option bypasses all filters in the pipeline, including the bias
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
514 filter.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
515
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
516 The --nobias option turns off (bypasses) the biased composition filter. The
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
517 simple null model is used as a null hypothesis for MSV and in subsequent filter
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
518 steps. The biased composition filter step compromises a small amount of
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
519 sensitivity. Though it is good to have it on by default, you may want to shut
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
520 it off if you know you will have no problem with biased composition hits.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
521
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
522
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
523 **Advanced Documentation**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
524
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
525 A more detailed look at the internals of the various filter pipelines was
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
526 posted on the `developer's blog <http://selab.janelia.org/people/eddys/blog/?p=508>`__.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
527 The information posted there may be useful to those who are struggling with
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
528 poor-scoring sequences.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
529
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
530 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
531 <token name="@ADV_OPTS_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
532 Advanced Options
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
533 ----------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
534
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
535 **nonull2**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
536
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
537 can be too aggressive sometimes, causing you to miss homologs. You can turn the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
538 biased-composition score correction off with the --nonull2 option (and if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
539 you’re doing that, you may also want to set --nobias, to turn off another
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
540 biased composition step called the bias filter, which affects which sequences
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
541 get scored at all).
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
542
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
543 **domZ**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
544
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
545 Assert that the total number of targets in your searches is <x>, for the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
546 purposes of per-domain conditional E-value calculations, rather than the number
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
547 of targets that passed the reporting thresholds.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
548
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
549 **Z**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
550
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
551 Assert that the total number of targets in your searches is <x>, for the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
552 purposes of per-sequence E-value calculations, rather than the actual number of
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
553 targets seen.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
554 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
555 <token name="@AEEWS_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
556 Effective Sequence Number
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
557 -------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
558
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
559 After relative weights are determined, they are normalized to sum to a total
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
560 effective sequence number, eff nseq. This number may be the actual number of
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
561 sequences in the alignment, but it is almost always smaller than that. The
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
562 default entropy weighting method (--eent) reduces the effective sequence num-
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
563 ber to reduce the information content (relative entropy, or average expected
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
564 score on true homologs) per consensus position. The target relative entropy is
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
565 controlled by a two-parameter function, where the two parameters are settable
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
566 with --ere and --esigma.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
567
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
568 **--eent**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
569
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
570 Adjust effective sequence number to achieve a specific relative entropy per
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
571 position (see --ere). This is the default.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
572
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
573 **--eclust**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
574
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
575 Set effective sequence number to the number of single-linkage clusters at a
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
576 specific identity threshold (see --eid). This option is not recommended; it’s
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
577 for experiments evaluating how much better --eent is.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
578
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
579 **--enone**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
580
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
581 Turn off effective sequence number determination and just use the actual number
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
582 of sequences. One reason you might want to do this is to try to maximize the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
583 relative entropy/position of your model, which may be useful for short models.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
584
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
585 **--eset**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
586
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
587 Explicitly set the effective sequence number for all models to <x>.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
588
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
589 **--ere**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
590
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
591 Set the minimum relative entropy/position target to <x>. Requires --eent. Default
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
592 depends on the sequence alphabet. For protein sequences, it is 0.59 bits/position;
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
593 for nucleotide sequences, it is 0.45 bits/position.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
594
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
595 **--esigma**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
596
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
597 Sets the minimum relative entropy contributed by an entire model alignment, over
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
598 its whole length. This has the effect of making short models have higher relative
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
599 entropy per position than --ere alone would give. The default is 45.0 bits.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
600
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
601 **--eid**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
602
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
603 Sets the fractional pairwise identity cutoff used by single linkage clustering
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
604 with the --eclust option. The default is 0.62.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
605 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
606 <token name="@ARSWS_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
607 Options Controlling Relative Weights
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
608 ------------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
609
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
610 HMMER uses an ad hoc sequence weighting algorithm to downweight closely related
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
611 sequences and up-weight distantly related ones. This has the effect of making
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
612 models less biased by uneven phylogenetic representation. For example, two
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
613 identical sequences would typically each receive half the weight that one
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
614 sequence would. These options control which algorithm gets used.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
615
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
616
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
617 **--wpb**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
618
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
619 Use the Henikoff position-based sequence weighting scheme [Henikoff and
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
620 Henikoff, J. Mol. Biol. 243:574, 1994]. This is the default.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
621
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
622 **--wgsc**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
623
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
624 Use the Gerstein/Sonnhammer/Chothia weighting algorithm [Gerstein et al, J.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
625 Mol. Biol. 235:1067, 1994].
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
626
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
627 **--wblosum**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
628
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
629 Use the same clustering scheme that was used to weight data in calculating
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
630 BLOSUM subsitution matrices [Henikoff and Henikoff, Proc. Natl. Acad. Sci
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
631 89:10915, 1992]. Sequences are single-linkage clustered at an identity
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
632 threshold (default 0.62; see --wid) and within each cluster of c sequences,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
633 each sequence gets rela- tive weight 1/c.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
634
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
635 **--wnone**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
636
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
637 No relative weights. All sequences are assigned uniform weight.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
638
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
639 **--wid**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
640
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
641 Sets the identity threshold used by single-linkage clustering when using
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
642 --wblosum. Invalid with any other weighting scheme. Default is 0.62.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
643 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
644 <token name="@BIAS_COMP_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
645 Bias Composition
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
646 ----------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
647
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
648 The next number, the bias, is a correction term for biased sequence composition
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
649 that has been applied to the sequence bit score.1 For instance, for the top hit
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
650 MYG PHYCA that scored 222.7 bits, the bias of 3.2 bits means that this sequence
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
651 originally scored 225.9 bits, which was adjusted by the slight 3.2 bit biased-
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
652 composition correction. The only time you really need to pay attention to the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
653 bias value is when it’s large, on the same order of magnitude as the sequence
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
654 bit score. Sometimes (rarely) the bias correction isn’t aggressive enough, and
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
655 allows a non-homolog to retain too much score. Conversely, the bias correction
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
656 can be too aggressive sometimes, causing you to miss homologs. You can turn the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
657 biased-composition score correction off with the --nonull2 option (and if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
658 you’re doing that, you may also want to set --nobias, to turn off another
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
659 biased composition step called the bias filter, which affects which sequences
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
660 get scored at all).
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
661
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
662 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
663 <token name="@CUT_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
664 Options for Model-specific Score Thresholding
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
665 ---------------------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
666
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
667 Curated profile databases may define specific bit score thresholds for each
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
668 profile, superseding any thresholding based on statistical significance alone.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
669 To use these options, the profile must contain the appropriate (GA, TC, and/or
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
670 NC) optional score threshold annotation; this is picked up by hmmbuild from
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
671 Stockholm format alignment files. Each thresholding option has two scores: the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
672 per-sequence threshold <x1> and the per-domain threshold <x2> These act as if
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
673 -T<x1> --incT<x1> --domT<x2> --incdomT<x2> has been applied specifically using
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
674 each model’s curated thresholds.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
675
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
676 **--cut_ga**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
677
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
678 Use the GA (gathering) bit scores in the model to set per-sequence (GA1) and
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
679 per-domain (GA2) reporting and inclusion thresholds. GA thresholds are
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
680 generally considered to be the reliable curated thresholds defining family
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
681 membership; for example, in Pfam, these thresholds define what gets included in
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
682 Pfam Full alignments based on searches with Pfam Seed models.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
683
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
684 **--cut_nc**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
685
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
686 Use the NC (noise cutoff) bit score thresholds in the model to set
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
687 per-sequence (NC1) and per-domain (NC2) reporting and inclusion thresholds. NC
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
688 thresholds are generally considered to be the score of the highest-scoring
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
689 known false positive.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
690
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
691 **--cut_tc**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
692
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
693 Use the NC (trusted cutoff) bit score thresholds in the model to set
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
694 per-sequence (TC1) and per-domain (TC2) reporting and inclusion thresholds. TC
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
695 thresholds are generally considered to be the score of the lowest-scoring known
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
696 true positive that is above all known false positives.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
697 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
698 <token name="@EVAL_CALIB_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
699 Options Controlling H3 Parameter Estimation Methods
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
700 ---------------------------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
701
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
702 H3 uses three short random sequence simulations to estimating the location
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
703 parameters for the expected score distributions for MSV scores, Viterbi scores,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
704 and Forward scores. These options allow these simulations to be modified.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
705
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
706 **--EmL**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
707
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
708 Sets the sequence length in simulation that estimates the location parameter mu
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
709 for MSV E-values. Default is 200.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
710
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
711 **--EmN**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
712
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
713 Sets the number of sequences in simulation that estimates the location parameter
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
714 mu for MSV E-values. Default is 200.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
715
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
716 **--EvL**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
717
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
718 Sets the sequence length in simulation that estimates the location parameter mu
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
719 for Viterbi E-values. Default is 200.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
720
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
721 **--EvN**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
722
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
723 Sets the number of sequences in simulation that estimates the location parameter
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
724 mu for Viterbi E-values. Default is 200.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
725
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
726
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
727 **--EfL**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
728
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
729 Sets the sequence length in simulation that estimates the location parameter tau
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
730 for Forward E-values. Default is 100.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
731
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
732 **--EfN**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
733
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
734 Sets the number of sequences in simulation that estimates the location parameter
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
735 tau for Forward E-values. Default is 200.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
736
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
737 **--Eft**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
738
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
739 Sets the tail mass fraction to fit in the simulation that estimates the location param-
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
740 eter tau for Forward evalues. Default is 0.04.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
741 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
742 <token name="@FORMAT_SELECTOR_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
743 Options for Specifying the Alphabet
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
744 -----------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
745
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
746 The alphabet type (amino, DNA, or RNA) is autodetected by default, by looking
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
747 at the composition of the msafile. Autodetection is normally quite reliable,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
748 but occasionally alphabet type may be ambiguous and autodetection can fail (for
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
749 instance, on tiny toy alignments of just a few residues). To avoid this, or to
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
750 increase robustness in automated analysis pipelines, you may specify the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
751 alphabet type of msafile with these options.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
752 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
753 <token name="@HSSI_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
754 Options Controlling Single Sequence Scoring (first Iteration)
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
755 -------------------------------------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
756
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
757 By default, the first iteration uses a search model constructed from a single
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
758 query sequence. This model is constructed using a standard 20x20 substitution
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
759 matrix for residue probabilities, and two additional pa- rameters for
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
760 position-independent gap open and gap extend probabilities. These options allow
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
761 the default single-sequence scoring parameters to be changed.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
762
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
763 **Gap Open (--popen)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
764
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
765 Set the gap open probability for a single sequence query model to <x>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
766
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
767 **Gap Extend (--pextend)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
768
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
769 Set the gap extend probability for a single sequence query model to <x>.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
770
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
771
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
772 **--mx/--mxfile**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
773
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
774 These options are not currently supported
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
775 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
776 <token name="@LENGTHS_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
777 Tail Mass Options
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
778 -----------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
779
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
780 **Window length tail mass (--w_beta)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
781
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
782 The upper bound, W, on the length at which nhmmer expects to find an instance
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
783 of the model is set such that the fraction of all sequences generated by the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
784 model with length >= W is less than <x>. The default is 1e-7.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
785
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
786
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
787 **Model instance length upper bound (--w length)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
788
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
789 Override the model instance length upper bound, W, which is otherwise
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
790 controlled by --w beta. It should be larger than the model length. The value of
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
791 W is used deep in the acceleration pipeline, and modest changes are not
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
792 expected to impact results (though larger values of W do lead to longer run
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
793 time).
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
794
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
795 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
796 <token name="@MCSS_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
797 **Options Controlling Profile Construction**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
798
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
799 These options control how consensus columns are defined in an alignment.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
800
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
801 **--fast**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
802
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
803 Define consensus columns as those that have a fraction >= symfrac of residues
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
804 as opposed to gaps. (See below for the --symfrac option.) This is the default.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
805
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
806 **--hand**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
807
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
808 Define consensus columns in next profile using reference annotation to the multiple
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
809 alignment. This allows you to define any consensus columns you like.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
810
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
811
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
812 **--symfrac**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
813
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
814 Define the residue fraction threshold necessary to define a consensus column
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
815 when using the --fast option. The default is 0.5. The symbol fraction in each
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
816 column is calculated after taking relative sequence weighting into account, and
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
817 ignoring gap characters corresponding to ends of sequence fragments (as opposed
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
818 to internal insertions/deletions). Setting this to 0.0 means that every
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
819 alignment column will be assigned as consensus, which may be useful in some
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
820 cases. Setting it to 1.0 means that only columns that include 0 gaps (internal
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
821 insertions/deletions) will be assigned as consensus.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
822
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
823 **--fragthresh**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
824
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
825 We only want to count terminal gaps as deletions if the aligned sequence is
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
826 known to be full-length, not if it is a fragment (for instance, because only
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
827 part of it was sequenced). HMMER uses a simple rule to infer fragments: if the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
828 sequence length L is less than or equal to a fraction <x> times the alignment
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
829 length in columns, then the sequence is handled as a fragment. The default is
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
830 0.5. Setting --fragthresh0 will define no (nonempty) sequence as a fragment;
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
831 you might want to do this if you know you’ve got a carefully curated alignment
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
832 of full-length sequences. Setting --fragthresh1 will define all sequences as
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
833 fragments; you might want to do this if you know your alignment is entirely
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
834 composed of fragments, such as translated short reads in metagenomic shotgun
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
835 data.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
836
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
837 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
838 <token name="@OFORMAT_WITH_OPTS_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
839 Options for Controlling Output
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
840 ------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
841
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
842 **Table of hits**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
843
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
844 Save a simple tabular (space-delimited) file summarizing the per-target output, with
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
845 one data line per homologous target model found.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
846
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
847 **Table of per-domain hits**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
848
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
849 Save a simple tabular (space-delimited) file summarizing the per-domain output,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
850 with one data line per homologous domain detected in a query sequence for each
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
851 homologous model.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
852
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
853 **Table of hits and domains in Pfam Format**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
854
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
855 Save an especially succinct tabular (space-delimited) file summarizing the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
856 per-target output, with one data line per homologous target model found.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
857 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
858 <token name="@OFORMAT_WITH_OPTS_NOPFAM_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
859 Options for Controlling Output
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
860 ------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
861
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
862 **Table of hits**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
863
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
864 Save a simple tabular (space-delimited) file summarizing the per-target output, with
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
865 one data line per homologous target model found.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
866
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
867 **Table of per-domain hits**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
868
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
869 Save a simple tabular (space-delimited) file summarizing the per-domain output,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
870 with one data line per homologous domain detected in a query sequence for each
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
871 homologous model.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
872 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
873 <token name="@OFORMAT_WITH_OPTS_N_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
874 Options for Controlling Output
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
875 ------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
876
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
877 **Table of hits**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
878
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
879 Save a simple tabular (space-delimited) file summarizing the per-target output, with
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
880 one data line per homologous target model found.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
881
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
882 **Table of hits (dfam)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
883
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
884 Save a tabular (space-delimited) file summarizing the per-hit output, similar
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
885 to --tblout but more succinct.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
886
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
887
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
888 **List of per-position scores for each hit (--aliscoreout)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
889
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
890 Save to file a list of per-position scores for each hit. This is useful, for
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
891 example, in identifying regions of high score density for use in resolving
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
892 overlapping hits from different models.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
893
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
894 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
895 <token name="@PRIOR_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
896 Options Controlling Priors
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
897 --------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
898
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
899 By default, weighted counts are converted to mean posterior probability
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
900 parameter estimates using mixture Dirichlet priors. Default mixture Dirichlet
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
901 prior parameters for protein models and for nucleic acid (RNA and DNA) models
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
902 are built in. The following options allow you to override the default priors.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
903
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
904 **No priors (--pnone)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
905
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
906 Don’t use any priors. Probability parameters will simply be the observed
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
907 frequencies, after relative sequence weighting.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
908
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
909 **Laplace +1 prior**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
910
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
911 Use a Laplace +1 prior in place of the default mixture Dirichlet prior.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
912 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
913 <token name="@SEED_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
914 Random Seeding
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
915 --------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
916
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
917 Seed the random number generator with <n>, an integer >= 0. If <n> is nonzero,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
918 any stochastic simulations will be reproducible; the same command will give the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
919 same results. If <n> is 0, the random number generator is seeded arbitrarily,
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
920 and stochastic simulations will vary from run to run of the same command.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
921
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
922 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
923 <token name="@THRESHOLDS_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
924 Options for Reporting Thresholds
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
925 --------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
926
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
927 Reporting thresholds control which hits are reported in output files (the main
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
928 output, --tblout, and --domtblout).
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
929
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
930 **E-value (-E)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
931
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
932 In the per-target output, report target profiles with an E-value of <= <x>. The
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
933 default is 10.0, meaning that on average, about 10 false positives will be
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
934 reported per query, so you can see the top of the noise and decide for yourself
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
935 if it’s really noise.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
936
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
937 **Bit score (-T)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
938
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
939 Instead of thresholding per-profile output on E-value, instead report target profiles
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
940 with a bit score of >= <x>.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
941
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
942 **domain E-value (--domE)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
943
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
944 In the per-domain output, for target profiles that have already satisfied the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
945 per-profile reporting threshold, report individual domains with a conditional
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
946 E-value of <= <x>. The default is 10.0. A conditional E-value means the
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
947 expected number of additional false positive domains in the smaller search
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
948 space of those comparisons that already satisfied the per-profile reporting
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
949 threshold (and thus must have at least one homologous domain already).
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
950
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
951 **domain Bit scores (--domT)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
952
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
953 Instead of thresholding per-domain output on E-value, instead report domains
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
954 with a bit score of >= <x>.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
955
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
956 Options for Inclusion Thresholds
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
957 --------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
958
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
959 Inclusion thresholds are stricter than reporting thresholds. Inclusion
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
960 thresholds control which hits are considered to be reliable enough to be
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
961 included in an output alignment or a subsequent search round. In hmmscan, which
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
962 does not have any alignment output (like hmmsearch or phmmer) nor any iterative
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
963 search steps (like jackhmmer), inclusion thresholds have little effect. They
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
964 only affect what domains get marked as significant (!) or questionable (?) in
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
965 domain output.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
966
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
967 **E-value of per target inclusion threshold**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
968
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
969 Use an E-value of <= <x> as the per-target inclusion threshold. The default is
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
970 0.01, meaning that on average, about 1 false positive would be expected in
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
971 every 100 searches with different query sequences.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
972
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
973 **Bit score of per target inclusion threshold**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
974
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
975 Instead of using E-values for setting the inclusion threshold, instead use a
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
976 bit score of >= <x> as the per-target inclusion threshold. It would be unusual
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
977 to use bit score thresholds with hmmscan, because you don’t expect a single
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
978 score threshold to work for different profiles; different profiles have
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
979 slightly different expected score distributions.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
980
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
981 **domain E-value per target inclusion treshold**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
982
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
983 Use a conditional E-value of <= <x> as the per-domain inclusion threshold, in
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
984 targets that have already satisfied the overall per-target inclusion threshold.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
985
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
986 **domain Bit score per target inclusion treshold**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
987
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
988 Instead of using E-values, instead use a bit score of >= <x> as the per-domain
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
989 inclusion threshold. As with --incT above, it would be unusual to use a single
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
990 bit score threshold in hmmscan.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
991
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
992 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
993 <token name="@THRESHOLDS_NODOM_HELP@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
994 Options for Reporting Thresholds
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
995 --------------------------------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
996
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
997 Reporting thresholds control which hits are reported in output files (the main
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
998 output, --tblout, and --domtblout).
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
999
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1000 **E-value (-E)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1001
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1002 In the per-target output, report target profiles with an E-value of <= <x>. The
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1003 default is 10.0, meaning that on average, about 10 false positives will be
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1004 reported per query, so you can see the top of the noise and decide for yourself
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1005 if it’s really noise.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1006
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1007 **Bit score (-T)**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1008
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1009 Instead of thresholding per-profile output on E-value, instead report target profiles
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1010 with a bit score of >= <x>.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1011
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1012 Options for Inclusion Thresholds
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1013 --------------------------------
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1014
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1015 Inclusion thresholds are stricter than reporting thresholds. Inclusion
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1016 thresholds control which hits are considered to be reliable enough to be
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1017 included in an output alignment or a subsequent search round. In hmmscan, which
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1018 does not have any alignment output (like hmmsearch or phmmer) nor any iterative
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1019 search steps (like jackhmmer), inclusion thresholds have little effect. They
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1020 only affect what domains get marked as significant (!) or questionable (?) in
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1021 domain output.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1022
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1023 **E-value of per target inclusion threshold**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1024
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1025 Use an E-value of <= <x> as the per-target inclusion threshold. The default is
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1026 0.01, meaning that on average, about 1 false positive would be expected in
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1027 every 100 searches with different query sequences.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1028
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iuc
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1029 **Bit score of per target inclusion threshold**
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1030
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1031 Instead of using E-values for setting the inclusion threshold, instead use a
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1032 bit score of >= <x> as the per-target inclusion threshold. It would be unusual
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1033 to use bit score thresholds with hmmscan, because you don’t expect a single
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1034 score threshold to work for different profiles; different profiles have
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1035 slightly different expected score distributions.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1036
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1037 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1038 <token name="@ATTRIBUTION@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1039
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iuc
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1040 Attribution
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1041 -----------
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1042
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iuc
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1043 This Galaxy tool relies on HMMER3_ from http://hmmer.janelia.org/
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1044 Internally the software is cited as:
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1045
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1046 ::
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1047
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iuc
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1048 # hmmscan :: search sequence(s) against a profile database
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1049 # HMMER 3.1 (February 2013); http://hmmer.org/
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iuc
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1050 # Copyright (C) 2011 Howard Hughes Medical Institute.
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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1051 # Freely distributed under the GNU General Public License (GPLv3).
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1052 # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
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iuc
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diff changeset
1053
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1054 The wrappers were written by Eric Rasche and is licensed under Apache2_. The
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
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diff changeset
1055 documentation is copied from the HMMER3 documentation.
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diff changeset
1056
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1057 .. _Apache2: http://www.apache.org/licenses/LICENSE-2.0
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iuc
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1058 .. _HMMER3: http://hmmer.janelia.org/
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diff changeset
1059
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diff changeset
1060
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diff changeset
1061 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1062 <token name="@HELP_PRE@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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parents:
diff changeset
1063
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diff changeset
1064 What it does
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1065 ============
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1066 ]]></token>
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
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1067 <token name="@HELP_PRE_OTH@"><![CDATA[
62479bdcc059 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hmmer3 commit 4164b44c651bcbdac6637eccce61b2a802c9b569
iuc
parents:
diff changeset
1068 Options
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iuc
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1069 =======
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1070 ]]></token>
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1071 </macros>