Mercurial > repos > iuc > idba_hybrid
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/idba_ud commit 9c0a7395ca18043ae2dd957f39f47b65c938750a
author | iuc |
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date | Thu, 21 Jul 2022 13:35:37 +0000 |
parents | a81c5169e9fc |
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<macros> <token name="@IDBA_VERSION@">1.1.3</token> <xml name="requirements"> <requirements> <requirement type="package" version="@IDBA_VERSION@">idba</requirement> <yield/> </requirements> </xml> <xml name="bio_tools"> <xrefs> <xref type="bio.tools">idba</xref> </xrefs> </xml> <xml name="main_input"> <param argument="--read" type="data" format="fasta" label="Fasta read file. Lower or equal to 600b"/> <param argument="--long_read" type="data" format="fasta" optional="true" label="Fasta long read file. More than 600b"/> </xml> <token name="@MAIN_INPUT@"> --read '$read' #if $long_read: --long_read '$long_read' #end if </token> <xml name="levels_input"> <param argument="--read_level_2" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for second level scaffolds"/> <param argument="--read_level_3" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for third level scaffolds"/> <param argument="--read_level_4" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for fourth level scaffolds"/> <param argument="--read_level_5" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for fifth level scaffolds"/> </xml> <token name="@LEVELS_INPUT@"> #if $read_level_2: --read_level_2 '$read_level_2' #end if #if $read_level_3: --read_level_3 '$read_level_3' #end if #if $read_level_4: --read_level_4 '$read_level_4' #end if #if $read_level_5: --read_level_5 '$read_level_5' #end if </token> <xml name="kmer_options" token_maxk_default="" token_step_default=""> <param argument="--mink" type="integer" value="20" max="312" label="Minimum k value. Lower or equal to 312"/> <param argument="--maxk" type="integer" value="@MAXK_DEFAULT@" max="312" label="Maximum k value. Lower or equal to 312"/> <param argument="--step" type="integer" value="@STEP_DEFAULT@" label="Increment of k-mer of each iteration"/> <param argument="--inner_mink" type="integer" value="10" label="Inner minimum k value"/> <param argument="--inner_step" type="integer" value="5" label="Inner increment of k-mer"/> <param argument="--prefix" type="integer" value="3" label="Prefix length used to build sub k-mer table"/> <param argument="--min_count" type="integer" value="2" label="Minimum multiplicity for filtering k-mer when building the graph"/> <param argument="--min_support" type="integer" value="1" label="Minimum supoort in each iteration"/> <param argument="--seed_kmer" type="integer" value="30" label="Seed kmer size for alignment"/> </xml> <token name="@KMER_OPTIONS@"> --mink $mink --maxk $maxk --step $step --inner_mink $inner_mink --inner_step $inner_step --prefix $prefix --min_count $min_count --min_support $min_support --seed_kmer $seed_kmer </token> <xml name="filter_options"> <param argument="--min_contig" type="integer" value="200" label="Minimum size of contig"/> <param argument="--similar" type="float" value="0.95" label="Similarity for alignment"/> <param argument="--max_mismatch" type="integer" value="3" label="Max mismatch of error correction"/> </xml> <token name="@FILTER_OPTIONS@"> --min_contig $min_contig --similar $similar --max_mismatch $max_mismatch </token> <xml name="min_pairs_filter"> <param argument="--min_pairs" type="integer" value="3" label="Minimum number of pairs"/> </xml> <token name="@MIN_PAIRS_FILTER@"> --min_pairs $min_pairs </token> <xml name="other_options"> <param name="other" type="select" display="checkboxes" multiple="true" label="Other options"> <yield/> <option value="--no_local">Do not use local assembly (--no_local)</option> <option value="--no_coverage">Do not iterate on coverage (--no_coverage)</option> <option value="--no_correct">Do not do correction (--no_correct)</option> <option value="--pre_correction">Perform pre-correction before assembly (--pre_correction)</option> </param> </xml> <token name="@OTHER_OPTIONS@"> #if $other: ${" ".join(str($other).split(","))} #end if </token> <token name="@THREADS@"> --num_threads \${GALAXY_SLOTS:-1} </token> <xml name="help" token_more_help=""> <help><![CDATA[ IDBA is an iterative De Bruijn Graph De Novo Assembler for sequence assembly. Most assemblers based on de Bruijn graph build a de Bruijn graph with a specific k-mer size to perform the assembling task. For all of them, it is very crucial to find a specific value of k. If k is too large, there will be a lot of gap problems in the graph. If k is too small, there will a lot of branch problems. IDBA uses not only one specific k but a range of k values to build the iterative de Bruijn graph. It can keep all the information in graphs with different k values. @MORE_HELP@ Input: IDBA-* take interleaved paired end data in the FASTA format as input, i.e. paired-end reads need to be stored in the same FASTA file such that a pair of reads should be in two consecutive lines. In Galaxy paired reads in separate FASTQ files can be converted into interleaved FASTA using the tools: * `FASTQ interlacer on paired end read <https://toolshed.g2.bx.psu.edu/view/devteam/fastq_paired_end_interlacer>`_ * `Samtools extract FASTA or FASTQ from a SAM file <https://toolshed.g2.bx.psu.edu/view/devteam/fastq_to_fasta>`_ Note that, IDBA-* assumes that the paired-end reads are in order (->,<-). If your data is in reverse order (<-,->), please convert it by yourself. ]]></help> </xml> <xml name="citations"> <citations> <citation type="doi">10.1007/978-3-642-12683-3_28</citation> <yield/> </citations> </xml> </macros>