changeset 4:8705e0d74a0f draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/last commit 6b601baaf89634bc8d110ea06e3e64c7a585f3ba"
author iuc
date Wed, 24 Mar 2021 22:36:50 +0000
parents 3de2195f32c0
children f5a5a2b39ff2
files lastal.xml lastdb.xml lastsplit.xml lasttrain.xml macros_last.xml maf-convert.xml test-data/fuguMito.fa.gz test-data/fuguMito.fq.gz test-data/humanMito.fa.gz test-data/humanMito.fq.gz test-data/last_align_fastq.maf test-data/last_align_gen.maf test-data/last_align_prot.maf test-data/last_split.maf test-data/last_train.txt test-data/last_train_E.txt
diffstat 16 files changed, 614 insertions(+), 485 deletions(-) [+]
line wrap: on
line diff
--- a/lastal.xml	Fri Nov 13 09:05:49 2020 +0000
+++ b/lastal.xml	Wed Mar 24 22:36:50 2021 +0000
@@ -1,4 +1,4 @@
-<tool id="last_al" name="LASTal" version="@LAST_CONDA_VERSION@+galaxy1" profile="18.01">
+<tool id="last_al" name="LASTal" version="@LAST_CONDA_VERSION@+galaxy0" profile="20.01">
 
     <description>finds local alignments between query sequences, and reference sequences.</description>
 
@@ -11,21 +11,28 @@
     </requirements>
 
     <command detect_errors="exit_code"><![CDATA[
+        set -e -o pipefail; 
+
         #if $db_opts.db_opts_input == 'lastdb'
             ln -s '$db_opts.lastdatabase.extra_files_path' './db_files' &&
         #end if
-        
-        lastal
 
-        #if $query_seq.ext == 'fasta'
+        #if $query_seq.ext.endswith('.gz')
+        zcat 
+        #else
+        cat 
+        #end if
+        '$query_seq' | lastal
+
+        #if $query_seq.ext.startswith('fasta')
             -Q 0
-        #else if $query_seq.ext == 'fastqsanger'
+        #else if $query_seq.ext.startswith('fastqsanger')
             -Q 1
-        #else if $query_seq.ext == 'fastqsolexa'
+        #else if $query_seq.ext.startswith('fastqsolexa')
             -Q 2
-        #else if $query_seq.ext == 'fastqillumina'
+        #else if $query_seq.ext.startswith('fastqillumina')
             -Q 3
-        #else if $query_seq.ext == 'pssm'
+        #else if $query_seq.ext.startswith('pssm')
             -Q 5            
         #end if
 
@@ -79,14 +86,12 @@
             'db_files/lastdb'
         #end if
 
-        '$query_seq'
-
         >'$outfile'
     ]]></command>
 
     <inputs>
         <expand macro="input_db"/>
-        <param name="query_seq" type="data" format="fasta,fastqsanger,fastqsolexa,fastqillumina,tabular" label="Query sequences"/>
+        <param name="query_seq" type="data" format="fasta,fasta.gz,fastq,fastq.gz,tabular" label="Query sequences"/>
 
         <section name="lastal" title="Lastal arguments" expanded="true">
             <param argument="-f" type="select" multiple="false" label="Output format">
@@ -348,7 +353,7 @@
                 <param name="db_opts_input" value="db"/>
                 <param name="database" value="humdb"/>
             </conditional>
-            <param name="query_seq" value="fuguMito.fa" ftype="fasta"/>
+            <param name="query_seq" value="fuguMito.fa.gz" ftype="fasta.gz"/>
             <output name="outfile" ftype="maf" file="last_align_gen.maf" lines_diff="2"/>
         </test>
         <test>
@@ -369,7 +374,7 @@
                 <param name="db_opts_input" value="db"/>
                 <param name="database" value="humdb-sanger"/>
             </conditional>
-            <param name="query_seq" value="fuguMito.fq" ftype="fastqsanger"/>
+            <param name="query_seq" value="fuguMito.fq.gz" ftype="fastqsanger.gz"/>
             <section name="lastal">
                 <param name="Q" value="1"/>
             </section>
--- a/lastdb.xml	Fri Nov 13 09:05:49 2020 +0000
+++ b/lastdb.xml	Wed Mar 24 22:36:50 2021 +0000
@@ -1,4 +1,4 @@
-<tool id="last_db" name="LASTdb" version="@LAST_CONDA_VERSION@+galaxy1" profile="18.01">
+<tool id="last_db" name="LASTdb" version="@LAST_CONDA_VERSION@+galaxy0" profile="20.01">
 
     <description>prepares sequences for subsequent comparison and alignment using lastal.</description>
 
@@ -11,15 +11,22 @@
     </requirements>
 
     <command detect_errors="exit_code"><![CDATA[
-        lastdb
+        set -e -o pipefail; 
 
-        #if $input_files.ext == 'fasta'
+        #if $input_files.ext.endswith('.gz')
+        zcat 
+        #else
+        cat 
+        #end if
+        '$input_files' | lastdb
+
+        #if $input_files.ext.startswith('fasta')
             -Q 0
-        #else if $input_files.ext == 'fastqsanger'
+        #else if $input_files.ext.startswith('fastqsanger')
             -Q 1
-        #else if $input_files.ext == 'fastqsolexa'
+        #else if $input_files.ext.startswith('fastqsolexa')
             -Q 2
-        #else if $input_files.ext == 'fastqillumina'
+        #else if $input_files.ext.startswith('fastqillumina')
             -Q 3
         #end if
         $lastdb.sequences_type.protein 
@@ -29,14 +36,16 @@
         -S $lastdb.lastdb_advanced.S
         -u $lastdb.lastdb_advanced.seeds.u
         -w $lastdb.lastdb_advanced.seeds.w
-        -W $lastdb.lastdb_advanced.seeds.W 
+        #if $lastdb.lastdb_advanced.seeds.W
+            -W $lastdb.lastdb_advanced.seeds.W
+        #end if
         -m $lastdb.lastdb_advanced.seeds.m 
         -i $lastdb.lastdb_advanced.i 
         -C $lastdb.lastdb_advanced.C 
         -s \${GALAXY_MEMORY_MB:-2G}
         -P \${GALAXY_SLOTS:-1}        
         'lastdb' 
-        '$input_files'
+        
 
         &&
 
@@ -45,7 +54,7 @@
     ]]></command>
 
     <inputs>
-        <param name="input_files" type="data" format="fasta,fastqsanger,fastqsolexa,fastqillumina" label="Reference(s) input files" />
+        <param name="input_files" type="data" format="fasta,fasta.gz,fastq,fastq.gz" label="Reference(s) input files" />
         <section name="lastdb" title="Lastdb arguments" expanded="true">
             <conditional name="sequences_type">
                 <param name="protein" type="select" multiple="false" label="The sequences are :">
@@ -81,7 +90,7 @@
                 </param>
 
                 <conditional name="seeds">
-                    <param argument="-u" type="select" multiple="false" label="Specify a seeding scheme.">
+                    <param argument="-u" type="select" multiple="false" optional="true" label="Specify a seeding scheme.">
                         <option value="BISF">BISF</option>
                         <option value="BISR">BISR</option>
                         <option value="MAM4">MAM4</option>
@@ -134,7 +143,7 @@
 
     <tests>
         <test>
-            <param name="input_files" value="humanMito.fa" ftype="fasta"/>
+            <param name="input_files" value="humanMito.fa.gz" ftype="fasta.gz"/>
             <section name="lastdb">
                 <section name="repeats">
                     <param name="uppercase" value="0"/>
@@ -145,7 +154,7 @@
             <output name="outfile" compare="contains" file="humdb.lastdb" ftype="lastdb">
                 <extra_files type="file" value="humdb.bck" name="lastdb.bck" compare="sim_size" delta="1"/>
                 <extra_files type="file" value="humdb.des" name="lastdb.des"/>
-                <extra_files type="file" value="humdb.prj" name="lastdb.prj"/>
+                <extra_files type="file" value="humdb.prj" name="lastdb.prj" compare="sim_size" delta="2"/>
                 <extra_files type="file" value="humdb.sds" name="lastdb.sds"/>
                 <extra_files type="file" value="humdb.ssp" name="lastdb.ssp" compare="sim_size" delta="1"/>
                 <extra_files type="file" value="humdb.suf" name="lastdb.suf" compare="sim_size" delta="1"/>
@@ -159,6 +168,10 @@
                     <param name="protein" value="-p"/> 
                 </conditional>
             </section>
+            <section name="lastdb_advanced">
+                <conditional name="seeds">
+                    <param name="u" value="MURPHY10"/>
+            </section>
             <output name="outfile" compare="contains" file="hedgdb.lastdb" ftype="lastdb">
                 <extra_files type="file" value="hedgdb.bck" name="lastdb.bck" compare="sim_size" delta="1"/>
                 <extra_files type="file" value="hedgdb.des" name="lastdb.des"/>
@@ -170,7 +183,7 @@
             </output>
         </test>
         <test>
-            <param name="input_files" value="humanMito.fq" ftype="fastqsanger"/>
+            <param name="input_files" value="humanMito.fq.gz" ftype="fastqsanger.gz"/>
             <section name="lastdb">
                 <param name="Q" value="1"/>
                 <section name="repeats">
@@ -182,7 +195,7 @@
             <output name="outfile" compare="contains" file="hedgdb.lastdb" ftype="lastdb">
                 <extra_files type="file" value="humdb-sanger.bck" name="lastdb.bck" compare="sim_size" delta="1"/>
                 <extra_files type="file" value="humdb-sanger.des" name="lastdb.des"/>
-                <extra_files type="file" value="humdb-sanger.prj" name="lastdb.prj" lines_diff="1"/>
+                <extra_files type="file" value="humdb-sanger.prj" name="lastdb.prj" lines_diff="2"/>
                 <extra_files type="file" value="humdb-sanger.sds" name="lastdb.sds"/>
                 <extra_files type="file" value="humdb-sanger.ssp" name="lastdb.ssp" compare="sim_size" delta="1"/>
                 <extra_files type="file" value="humdb-sanger.suf" name="lastdb.suf" compare="sim_size" delta="1"/>
--- a/lastsplit.xml	Fri Nov 13 09:05:49 2020 +0000
+++ b/lastsplit.xml	Wed Mar 24 22:36:50 2021 +0000
@@ -1,4 +1,4 @@
-<tool id="last_split" name="LAST-split" version="@LAST_CONDA_VERSION@+galaxy0" profile="18.01">
+<tool id="last_split" name="LAST-split" version="@LAST_CONDA_VERSION@+galaxy0" profile="20.01">
 
     <description>finds "split alignments" (typically for DNA) or "spliced alignments" (typically for RNA).</description>
 
@@ -72,7 +72,7 @@
                 <param name="database" value="humdb"/>
             </conditional>
             <param name="last_align" ftype="maf" value="last_align_gen.maf"/>
-            <output name="outfile" ftype="maf" file="last_split.maf"/>
+            <output name="outfile" ftype="maf" file="last_split.maf"  lines_diff="2"/>
         </test>
     </tests>
 
--- a/lasttrain.xml	Fri Nov 13 09:05:49 2020 +0000
+++ b/lasttrain.xml	Wed Mar 24 22:36:50 2021 +0000
@@ -1,4 +1,4 @@
-<tool id="last_train" name="LAST-train" version="@LAST_CONDA_VERSION@+galaxy2" profile="18.01">
+<tool id="last_train" name="LAST-train" version="@LAST_CONDA_VERSION@+galaxy0" profile="20.01">
 
     <description>finds the rates (probabilities) of insertion, deletion, and substitutions between two sets of sequences.</description>
 
@@ -11,11 +11,16 @@
     </requirements>
 
     <command detect_errors="exit_code"><![CDATA[
+        set -e -o pipefail; 
         #if $db_opts.db_opts_input == 'lastdb'
             ln -s '$db_opts.lastdatabase.extra_files_path' './db_files' &&
         #end if
-
-        last-train
+        #if $query_fasta.ext.endswith('.gz')
+        zcat 
+        #else
+        cat 
+        #end if
+        '$query_fasta' | last-train
 
         -Q $init_options.Q
         -r $init_options.r
@@ -43,15 +48,13 @@
             'db_files/lastdb'
         #end if
 
-        '$query_fasta'
-
         >'$outfile'
 
     ]]></command>
 
     <inputs>
         <expand macro="input_db" />
-        <param name="query_fasta" type="data" format="fasta,fastqsanger" label="Queries input files" />
+        <param name="query_fasta" type="data" format="fasta,fasta.gz,fastqsanger,fastqsanger.gz" label="Queries input files" />
         <conditional name="init_options">            
             <param argument="-Q" type="select" multiple="false" label="Query input format">
                 <option value="0" selected="true">FASTA</option>
@@ -115,15 +118,15 @@
                     <param name="E" value="10"/>
                 </section>
             </section>
-            <param name="query_fasta" value="fuguMito.fa"/>
-            <output name="outfile" file="last_train_E.txt" ftype="txt" lines_diff="22"/>
+            <param name="query_fasta" value="fuguMito.fa.gz" ftype="fasta.gz"/>
+            <output name="outfile" file="last_train_E.txt" ftype="txt" lines_diff="28"/>
         </test>
         <test>
             <conditional name="db_opts">
                 <param name="db_opts_input" value="db"/>
                 <param name="database" value="humdb"/>
             </conditional>
-            <param name="query_fasta" value="fuguMito.fa"/>
+            <param name="query_fasta" value="fuguMito.fq.gz" ftype="fastqsanger.gz"/>
             <output name="outfile" file="last_train.txt" ftype="txt" lines_diff="22"/>
         </test>
     </tests>
--- a/macros_last.xml	Fri Nov 13 09:05:49 2020 +0000
+++ b/macros_last.xml	Wed Mar 24 22:36:50 2021 +0000
@@ -1,5 +1,5 @@
 <macros>
-    <token name="@LAST_CONDA_VERSION@">1021</token>
+    <token name="@LAST_CONDA_VERSION@">1205</token>
     <token name="@LAST_HELP@"><![CDATA[
         Documentation : http://last.cbrc.jp/
 
--- a/maf-convert.xml	Fri Nov 13 09:05:49 2020 +0000
+++ b/maf-convert.xml	Wed Mar 24 22:36:50 2021 +0000
@@ -1,4 +1,4 @@
-<tool id="last_maf_convert" name="MAF-convert" version="@LAST_CONDA_VERSION@+galaxy0" profile="18.01">
+<tool id="last_maf_convert" name="MAF-convert" version="@LAST_CONDA_VERSION@+galaxy0" profile="20.01">
 
     <description>read MAF-format alignments and write them in another format.</description>
 
Binary file test-data/fuguMito.fa.gz has changed
Binary file test-data/fuguMito.fq.gz has changed
Binary file test-data/humanMito.fa.gz has changed
Binary file test-data/humanMito.fq.gz has changed
--- a/test-data/last_align_fastq.maf	Fri Nov 13 09:05:49 2020 +0000
+++ b/test-data/last_align_fastq.maf	Wed Mar 24 22:36:50 2021 +0000
@@ -1,8 +1,8 @@
-# LAST version 1021
+# LAST version 1205
 #
 # a=7 b=1 A=7 B=1 e=22 d=13 x=21 y=9 z=21 D=1e+06
 # R=10 u=0 s=2 S=0 M=0 T=0 m=10 l=1 L=999999999 n=10 k=1 w=1000 t=2.82533 j=3 Q=1
-# db_files/lastdb
+# /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb-sanger
 # Reference sequences=1 normal letters=16571
 #
 #    A  R  N  D  C  Q  E  G  H  I  L  K  M  F  P  S  T  W  Y  V  B  J  Z  X  *
--- a/test-data/last_align_gen.maf	Fri Nov 13 09:05:49 2020 +0000
+++ b/test-data/last_align_gen.maf	Wed Mar 24 22:36:50 2021 +0000
@@ -1,8 +1,8 @@
-# LAST version 1021
+# LAST version 1205
 #
 # a=7 b=1 A=7 B=1 e=22 d=13 x=21 y=9 z=21 D=1e+06
 # R=10 u=0 s=2 S=0 M=0 T=0 m=10 l=1 L=999999999 n=10 k=1 w=1000 t=2.82533 j=3 Q=0
-# ref_db
+# /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb
 # Reference sequences=1 normal letters=16571
 #
 #    A  R  N  D  C  Q  E  G  H  I  L  K  M  F  P  S  T  W  Y  V  B  J  Z  X  *
--- a/test-data/last_align_prot.maf	Fri Nov 13 09:05:49 2020 +0000
+++ b/test-data/last_align_prot.maf	Wed Mar 24 22:36:50 2021 +0000
@@ -1,8 +1,8 @@
-# LAST version 1021
+# LAST version 1205
 #
 # a=11 b=2 A=11 B=2 e=22 d=13 x=21 y=9 z=21 D=1e+06 E=2.73494e+09
 # R=10 u=0 s=1 S=0 M=0 T=0 m=10 l=1 L=999999999 n=10 k=1 w=1000 t=3.08611 j=3 Q=0
-# ref_db
+# /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/hedgdb
 # Reference sequences=1 normal letters=411
 # lambda=0.305935 K=0.102404
 #
@@ -39,28 +39,4 @@
 # name start alnSize strand seqSize alignment
 #
 # batch 0
-a score=729 EG2=1.4e-80 E=1.2e-101
-s NP_002172.2    13 264 + 411 LVLLLLLVVPA----AWGCGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGGCFPAGAQVRLESGARVALSAVRPGDRVLAMGEDGSPTFSDVLIFLDREPHRLRAFQVIETQDPPRRLALTPAHLL
-s NP_001034065.1 66 265 + 471 LVALLLIVLPMVFSPAHSCGPGRGLGRHRA--RNLYPLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDEEGTGADRLMSKRCKEKLNVLAYSVMNEWPGIRLLVTESWDEDYHHGQESLHYEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSSISSHVHGCFTPESTALLESGVRKPLGELSIGDRVLSMTANGQAVYSEVILFMDRNLEQMQNFVQLHT-DGGAVLTVTPAHLV
-
-a score=131 EG2=0.4 E=4.1e-14
-s NP_002172.2    293 95 + 411 FASHVQPGQYVLVAGVPG--LQPARVAAVSTHVALGAYAPLTKHGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFHSLAWGSWTPG-EGVHWYPQLL
-s NP_001034065.1 344 96 + 471 FADRIEEKNQVLVRDVETGELRPQRVVKVGSVRSKGVVAPLTREGTIVVNSVAASCYAVINSQSLAHWGLAPMRLLSTLE--AWLPAKEQLHSSPKVV
-
-a score=41 EG2=3.7e+11 E=0.06
-s NP_002172.2    91 34 + 411 KDEENTGADRLMTQRCKDRLNSLAISVMNQWPGV
-s NP_001034065.1 44 34 + 471 QEETQTMRHIAHTQRCLSRLTSLVALLLIVLPMV
-
-a score=30 EG2=1.1e+13 E=1.8
-s NP_002172.2    180 18 + 411 ESKAHVHCSVKSEHSAAA
-s NP_001034065.1  20 18 + 471 DAKCHSSSSSSSSKSAAS
-
-a score=24 EG2=6.6e+13 E=12
-s NP_002172.2     26 23 + 411 GCGPGRVVGSRRRPPRKLVPLAY
-s NP_001034065.1 150 21 + 471 GTGADRLMSKRCK--EKLNVLAY
-
-a score=24 EG2=6.6e+13 E=12
-s NP_002172.2    106 27 + 411 CKDRLNSLAISVMNQWPGVKLRVTEGW
-s NP_001034065.1 399 27 + 471 CYAVINSQSLAHWGLAPMRLLSTLEAW
-
 # Query sequences=1
--- a/test-data/last_split.maf	Fri Nov 13 09:05:49 2020 +0000
+++ b/test-data/last_split.maf	Wed Mar 24 22:36:50 2021 +0000
@@ -1,8 +1,8 @@
-# LAST version 1021
+# LAST version 1205
 #
 # a=7 b=1 A=7 B=1 e=22 d=13 x=21 y=9 z=21 D=1e+06
 # R=10 u=0 s=2 S=0 M=0 T=0 m=10 l=1 L=999999999 n=10 k=1 w=1000 t=2.82533 j=3 Q=0
-# ref_db
+# /home/berntm/.planemo/planemo_tmp_3z8peizh/test-data/humdb
 # Reference sequences=1 normal letters=16571
 #
 #    A  R  N  D  C  Q  E  G  H  I  L  K  M  F  P  S  T  W  Y  V  B  J  Z  X  *
@@ -89,7 +89,7 @@
 s fuguMito  16146 196 + 16447 A-----GTGC---AGCGC-----GTCAATGGTTCA-TCAAGGTTGAA-CAT---TTTTTCT-TGGTTTATGGTA---ATGTTAATTAATGAATTAAGACAT---TATTTAAG------AATTACATTACTGATATCAAGGACATAA--ATAATAATACGAT-TCAACAATCATACAATTTCAcccccttcttctttttaaaaaaatt-aacgtataccccccctacccccc
 p                             333333336?????@@@@@@@@@@@@@@@@@@@@@@@@@@@@AAAAAABBBBBBCCDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDEEEEEEEEEEEEEEEEEEEEEEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEED@@:
 
-a score=255 mismap=0.516 acc=AG
+a score=255 mismap=0.515 acc=AG
 s humanMito  2030 114 + 16571 ATAGAATCTTAGTTCAA-CTTTAAATTTG-CCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGA-AAAAACCTTGTA
 s fuguMito  16342 104 + 16447 ctaAAAAATAGGAGAGACCTTTAAGTTTGAACCA-AG----CTCT----CCActt----aattaaatattcatcatattattatcatatattataatattataataatataattata
 p                             #####################################################################################################################
--- a/test-data/last_train.txt	Fri Nov 13 09:05:49 2020 +0000
+++ b/test-data/last_train.txt	Wed Mar 24 22:36:50 2021 +0000
@@ -1,43 +1,43 @@
-# lastal version: 1021
+# lastal version: 1205
 # maximum percent identity: 100
 # scale of score parameters: 4.5512
 # scale used while training: 91.024
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -r5 -q5 -a15 -b3 -A15 -B3 /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -r5 -q5 -a15 -b3 -A15 -B3 /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
-# aligned letter pairs: 14938.3
-# deletes: 664.27
-# inserts: 839.49
-# delOpens: 344.82
-# insOpens: 404.93
+# aligned letter pairs: 14938.4
+# deletes: 664.28
+# inserts: 839.52
+# delOpens: 344.93
+# insOpens: 404.94
 # alignments: 3
-# mean delete size: 1.92643
-# mean insert size: 2.07317
-# matchProb: 0.951966
-# delOpenProb: 0.0219742
-# insOpenProb: 0.0258048
-# delExtendProb: 0.480904
-# insExtendProb: 0.517648
+# mean delete size: 1.92584
+# mean insert size: 2.0732
+# matchProb: 0.951959
+# delOpenProb: 0.0219809
+# insOpenProb: 0.0258051
+# delExtendProb: 0.480746
+# insExtendProb: 0.517653
 
-# substitution percent identity: 71.9318
+# substitution percent identity: 71.9301
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3296.3         533.237        387.81         392.24        
-# C 553.59         3241.5         182.39         686.22        
-# G 232.54         102.399        1565.4         84.91         
-# T 335.173        558.68         147.16         2651          
+# A 3296.3         533.242        387.82         392.35        
+# C 553.59         3241.5         182.39         686.23        
+# G 232.64         102.401        1565.4         84.91         
+# T 335.174        558.781        147.17         2651          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.22048        0.0356667      0.0259395      0.0262358     
-# C 0.0370281      0.216815       0.0121996      0.0458993     
-# G 0.0155539      0.00684918     0.104705       0.00567939    
-# T 0.0224188      0.0373685      0.00984312     0.177318      
+# A 0.220475       0.0356662      0.0259396      0.0262426     
+# C 0.0370272      0.21681        0.0121993      0.0458989     
+# G 0.0155603      0.00684915     0.104703       0.00567926    
+# T 0.0224183      0.0373744      0.00984356     0.177314      
 
-# delExistCost: 343
+# delExistCost: 342
 # insExistCost: 341
-# delExtendCost: 64
+# delExtendCost: 65
 # insExtendCost: 58
 
 # score matrix (query letters = columns, reference letters = rows):
@@ -47,84 +47,84 @@
 # G    -89   -164    145   -167
 # T   -112    -66   -127     90
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.3148 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.1872 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
-# aligned letter pairs: 14338.3
-# deletes: 327.44
-# inserts: 474.13
-# delOpens: 166.73
-# insOpens: 216.45
+# aligned letter pairs: 14397.9
+# deletes: 322.93
+# inserts: 481.67
+# delOpens: 166.26
+# insOpens: 218.88
 # alignments: 10
-# mean delete size: 1.96389
-# mean insert size: 2.19048
-# matchProb: 0.973244
-# delOpenProb: 0.0113172
-# insOpenProb: 0.014692
-# delExtendProb: 0.490807
-# insExtendProb: 0.54348
+# mean delete size: 1.94232
+# mean insert size: 2.20061
+# matchProb: 0.973223
+# delOpenProb: 0.0112383
+# insOpenProb: 0.0147951
+# delExtendProb: 0.485152
+# insExtendProb: 0.545581
 
-# substitution percent identity: 70.6019
+# substitution percent identity: 70.5263
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3076.4         546.106        412.41         369.93        
-# C 561.42         2983.2         149.401        735.35        
-# G 245.82         80.868         1562.07        66.655        
-# T 326.724        603.44         120.838        2510.5        
+# A 3087.2         550.976        415.27         370.69        
+# C 567.6          2996           150.293        740.47        
+# G 246.31         81.558         1563.06        66.884        
+# T 329.603        608.12         121.409        2521.4        
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.214366       0.0380532      0.0287371      0.0257771     
-# C 0.0391203      0.207872       0.0104104      0.0512399     
-# G 0.017129       0.00563496     0.108846       0.00464458    
-# T 0.0227664      0.0420483      0.0084201      0.174934      
+# A 0.214138       0.0382175      0.0288045      0.0257123     
+# C 0.0393706      0.207812       0.0104248      0.0513615     
+# G 0.0170849      0.00565713     0.108419       0.0046393     
+# T 0.0228624      0.0421812      0.00842133     0.174893      
 
-# delExistCost: 405
-# insExistCost: 402
-# delExtendCost: 64
+# delExistCost: 404
+# insExistCost: 401
+# delExtendCost: 65
 # insExtendCost: 54
 
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
-# A     77    -81    -49   -104
-# C    -79     73   -142    -42
-# G    -80   -181    146   -186
-# T   -108    -52   -142     90
+# A     76    -81    -49   -104
+# C    -78     73   -142    -42
+# G    -79   -180    146   -186
+# T   -108    -52   -141     89
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.4973 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t90.4093 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
-# aligned letter pairs: 14657.7
-# deletes: 218.21
-# inserts: 385.36
-# delOpens: 102.2
-# insOpens: 152.161
+# aligned letter pairs: 14586.1
+# deletes: 207.97
+# inserts: 370.61
+# delOpens: 97.51
+# insOpens: 146.118
 # alignments: 10
-# mean delete size: 2.13513
-# mean insert size: 2.53258
-# matchProb: 0.982218
-# delOpenProb: 0.00684846
-# insOpenProb: 0.0101964
-# delExtendProb: 0.531644
-# insExtendProb: 0.605146
+# mean delete size: 2.13281
+# mean insert size: 2.53637
+# matchProb: 0.982843
+# delOpenProb: 0.00657043
+# insOpenProb: 0.00984574
+# delExtendProb: 0.531134
+# insExtendProb: 0.605736
 
-# substitution percent identity: 69.4007
+# substitution percent identity: 69.4001
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3091.6         591.394        434.35         391.72        
-# C 605.8          2996.8         154.6091       779.75        
-# G 260.46         83.802         1568.22        67.9713       
-# T 351.852        646.07         124.676        2532.5        
+# A 3071.2         586.39         432.58         390.03        
+# C 602.06         2979.4         154.2015       775.84        
+# G 260.13         83.439         1563.17        67.5164       
+# T 349.003        643.71         124.293        2522.3        
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.210577       0.0402814      0.0295847      0.0266811     
-# C 0.0412626      0.20412        0.0105308      0.0531108     
-# G 0.0177406      0.00570797     0.106816       0.0046297     
-# T 0.0239655      0.0440055      0.008492       0.172495      
+# A 0.21028        0.0401492      0.0296181      0.0267048     
+# C 0.0412221      0.203995       0.0105579      0.0531206     
+# G 0.0178107      0.00571294     0.107028       0.00462274    
+# T 0.0238957      0.0440738      0.00851015     0.172698      
 
-# delExistCost: 466
-# insExistCost: 457
+# delExistCost: 469
+# insExistCost: 460
 # delExtendCost: 57
 # insExtendCost: 45
 
@@ -132,175 +132,175 @@
 #        A      C      G      T
 # A     76    -75    -45   -100
 # C    -73     72   -140    -38
-# G    -75   -178    147   -185
+# G    -74   -178    147   -185
 # T   -103    -48   -140     89
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.5154 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.4064 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
-# aligned letter pairs: 14615.5
-# deletes: 176.132
-# inserts: 337.667
-# delOpens: 73.361
-# insOpens: 117.993
+# aligned letter pairs: 14614.3
+# deletes: 173.005
+# inserts: 333.783
+# delOpens: 71.881
+# insOpens: 116.037
 # alignments: 8
-# mean delete size: 2.40089
-# mean insert size: 2.86175
-# matchProb: 0.986477
-# delOpenProb: 0.00495152
-# insOpenProb: 0.00796397
-# delExtendProb: 0.583489
-# insExtendProb: 0.650564
+# mean delete size: 2.40683
+# mean insert size: 2.87652
+# matchProb: 0.986705
+# delOpenProb: 0.00485315
+# insOpenProb: 0.0078344
+# delExtendProb: 0.584515
+# insExtendProb: 0.652358
 
-# substitution percent identity: 68.8798
+# substitution percent identity: 68.8464
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3057.2         603.516        434.56         399.47        
-# C 620.42         2962.4         159.336        789.74        
-# G 264.31         86.899         1557.17        68.449        
-# T 353.847        650            125.897        2508.2        
+# A 3054           603.416        435.41         400.71        
+# C 620.59         2961.2         159.53         789.72        
+# G 265.6          86.864         1557.13        68.4163       
+# T 354.125        649.76         125.994        2505.1        
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.208805       0.0412198      0.0296802      0.0272836     
-# C 0.0423743      0.20233        0.0108826      0.0539388     
-# G 0.0180522      0.00593515     0.106354       0.00467503    
-# T 0.0241675      0.0443946      0.00859869     0.171309      
+# A 0.208641       0.0412238      0.0297461      0.0273755     
+# C 0.0423971      0.202301       0.0108987      0.0539516     
+# G 0.0181451      0.00593432     0.106379       0.00467402    
+# T 0.0241929      0.0443899      0.00860758     0.171142      
 
-# delExistCost: 514
-# insExistCost: 497
+# delExistCost: 516
+# insExistCost: 500
 # delExtendCost: 49
-# insExtendCost: 39
+# insExtendCost: 38
 
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
 # A     75    -72    -44    -98
-# C    -71     72   -137    -37
-# G    -73   -174    146   -184
+# C    -70     72   -136    -37
+# G    -72   -174    146   -184
 # T   -102    -46   -138     89
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.0742 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.2552 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
-# aligned letter pairs: 14806.2
-# deletes: 167.925
-# inserts: 326.866
-# delOpens: 62.871
-# insOpens: 105.559
+# aligned letter pairs: 14846.5
+# deletes: 168.42
+# inserts: 340.671
+# delOpens: 63.085
+# insOpens: 107.237
 # alignments: 7
-# mean delete size: 2.67095
-# mean insert size: 3.09652
-# matchProb: 0.988224
-# delOpenProb: 0.00419626
-# insOpenProb: 0.00704543
-# delExtendProb: 0.625601
-# insExtendProb: 0.677057
+# mean delete size: 2.66973
+# mean insert size: 3.1768
+# matchProb: 0.988132
+# delOpenProb: 0.00419872
+# insOpenProb: 0.00713732
+# delExtendProb: 0.62543
+# insExtendProb: 0.685218
 
-# substitution percent identity: 68.3956
+# substitution percent identity: 68.3619
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3074.6         626.457        444.21         418.05        
-# C 637.55         2977.3         164.69         809.88        
-# G 269.41         92.415         1559.3         69.29         
-# T 359.136        664.2          129.482        2527.2        
+# A 3085.6         630.559        444.71         420.06        
+# C 641.49         2985.3         165.39         812.21        
+# G 270.72         92.7           1563.4         69.63         
+# T 360.446        667.21         130.014        2532.3        
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.207419       0.042262       0.0299673      0.0282025     
-# C 0.0430104      0.200854       0.0111103      0.0546361     
-# G 0.0181749      0.0062345      0.105193       0.00467444    
-# T 0.024228       0.0448082      0.00873511     0.17049       
+# A 0.207481       0.0423998      0.029903       0.0282455     
+# C 0.0431348      0.200736       0.0111211      0.0546143     
+# G 0.0182037      0.0062333      0.105126       0.00468203    
+# T 0.024237       0.0448643      0.00874235     0.170276      
 
 # delExistCost: 546
-# insExistCost: 519
+# insExistCost: 521
 # delExtendCost: 42
-# insExtendCost: 35
+# insExtendCost: 34
 
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
-# A     75    -70    -43    -95
+# A     75    -70    -44    -95
 # C    -69     71   -134    -36
 # G    -71   -169    146   -183
 # T   -101    -45   -136     88
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.1177 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.0533 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
-# aligned letter pairs: 14896.9
-# deletes: 177.451
-# inserts: 344.879
-# delOpens: 59.29
-# insOpens: 103.311
+# aligned letter pairs: 14896.8
+# deletes: 179.049
+# inserts: 347.285
+# delOpens: 59.728
+# insOpens: 103.021
 # alignments: 5
-# mean delete size: 2.99293
-# mean insert size: 3.33826
-# matchProb: 0.988809
-# delOpenProb: 0.00393548
-# insOpenProb: 0.00685746
-# delExtendProb: 0.66588
-# insExtendProb: 0.700443
+# mean delete size: 2.99774
+# mean insert size: 3.37101
+# matchProb: 0.988799
+# delOpenProb: 0.00396454
+# insOpenProb: 0.00683818
+# delExtendProb: 0.666415
+# insExtendProb: 0.703353
 
-# substitution percent identity: 68.1858
+# substitution percent identity: 68.1898
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3084.6         635.337        445.51         422.24        
-# C 649.18         2997.2         169.11         816.24        
-# G 273.53         93.848         1562.4         71.64         
-# T 365.354        674.15         132.032        2532.3        
+# A 3083.6         634.137        445.41         422.54        
+# C 648.98         2997.2         169.11         816.24        
+# G 273.63         93.839         1562.4         71.58         
+# T 365.254        674.05         132.042        2532.3        
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206678       0.0425696      0.0298506      0.0282914     
-# C 0.0434971      0.200822       0.0113309      0.0546907     
-# G 0.0183274      0.00628811     0.104686       0.00480011    
-# T 0.0244799      0.0451702      0.00884656     0.169672      
+# A 0.206644       0.0424959      0.0298486      0.028316      
+# C 0.0434906      0.200854       0.0113327      0.0546993     
+# G 0.018337       0.0062885      0.104702       0.00479684    
+# T 0.024477       0.0451706      0.00884863     0.169699      
 
-# delExistCost: 567
-# insExistCost: 531
+# delExistCost: 566
+# insExistCost: 532
 # delExtendCost: 37
 # insExtendCost: 32
 
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
 # A     75    -70    -43    -95
-# C    -68     71   -132    -35
+# C    -68     71   -132    -36
 # G    -70   -168    146   -181
 # T   -100    -45   -135     88
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.5512 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.3763 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
-# aligned letter pairs: 14897.4
-# deletes: 183.45
-# inserts: 350.682
-# delOpens: 57.728
-# insOpens: 101.786
+# aligned letter pairs: 14897.3
+# deletes: 182.446
+# inserts: 350.271
+# delOpens: 57.786
+# insOpens: 101.442
 # alignments: 5
-# mean delete size: 3.17783
-# mean insert size: 3.44529
-# matchProb: 0.989012
-# delOpenProb: 0.00383246
-# insOpenProb: 0.00675739
-# delExtendProb: 0.68532
-# insExtendProb: 0.709748
+# mean delete size: 3.15727
+# mean insert size: 3.45292
+# matchProb: 0.989031
+# delOpenProb: 0.00383641
+# insOpenProb: 0.00673473
+# delExtendProb: 0.683271
+# insExtendProb: 0.71039
 
-# substitution percent identity: 68.1676
+# substitution percent identity: 68.1657
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3083.6         635.04         445.71         423.87        
-# C 650.27         2996.2         169.49         816.21        
-# G 272.96         94.054         1562.4         71.81         
-# T 365.777        674.06         132.018        2532.4        
+# A 3083.6         635.136        445.71         423.86        
+# C 650.36         2996.2         169.48         816.19        
+# G 272.95         94.064         1561.4         71.9          
+# T 365.571        674.06         131.918        2532.4        
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206594       0.0425463      0.0298616      0.0283983     
-# C 0.0435666      0.200739       0.0113555      0.0546843     
-# G 0.0182877      0.00630141     0.104677       0.00481111    
-# T 0.0245062      0.0451605      0.00884491     0.169665      
+# A 0.206609       0.0425557      0.0298637      0.0283997     
+# C 0.0435758      0.200753       0.0113556      0.0546868     
+# G 0.0182884      0.00630253     0.104618       0.00481749    
+# T 0.0244942      0.0451638      0.00883885     0.169677      
 
-# delExistCost: 578
-# insExistCost: 536
+# delExistCost: 577
+# insExistCost: 537
 # delExtendCost: 34
 # insExtendCost: 31
 
@@ -311,40 +311,40 @@
 # G    -71   -168    146   -180
 # T   -100    -45   -135     88
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.3765 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.3676 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
 # aligned letter pairs: 14897.3
-# deletes: 186.949
-# inserts: 354.265
-# delOpens: 56.756
-# insOpens: 101.214
+# deletes: 186.95
+# inserts: 354.262
+# delOpens: 56.767
+# insOpens: 100.893
 # alignments: 5
-# mean delete size: 3.29391
-# mean insert size: 3.50016
-# matchProb: 0.989113
-# delOpenProb: 0.00376834
-# insOpenProb: 0.00672015
-# delExtendProb: 0.696409
-# insExtendProb: 0.714299
+# mean delete size: 3.29329
+# mean insert size: 3.51126
+# matchProb: 0.989133
+# delOpenProb: 0.00376915
+# insOpenProb: 0.00669898
+# delExtendProb: 0.696352
+# insExtendProb: 0.715202
 
-# substitution percent identity: 68.1476
+# substitution percent identity: 68.1477
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
 # A 3081.6         633.831        445.71         424.07        
-# C 650.26         2986.2         169.38         816.09        
+# C 650.26         2986.2         169.38         816.08        
 # G 272.84         94.051         1561.4         71.9          
-# T 365.569        674.07         131.791        2532.4        
+# T 365.569        674.17         131.691        2532.4        
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206664       0.0425072      0.029891       0.0284398     
-# C 0.0436089      0.200266       0.0113593      0.0547301     
-# G 0.0182977      0.00630742     0.104714       0.00482189    
-# T 0.0245165      0.0452057      0.00883841     0.169833      
+# A 0.206664       0.0425072      0.0298911      0.0284398     
+# C 0.043609       0.200266       0.0113593      0.0547295     
+# G 0.0182977      0.00630743     0.104714       0.00482189    
+# T 0.0245165      0.0452125      0.00883171     0.169833      
 
-# delExistCost: 584
-# insExistCost: 539
+# delExistCost: 583
+# insExistCost: 540
 # delExtendCost: 33
 # insExtendCost: 30
 
@@ -355,39 +355,39 @@
 # G    -71   -168    146   -180
 # T   -100    -45   -135     88
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.5205 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.511 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
 # aligned letter pairs: 14897.4
-# deletes: 188.951
-# inserts: 357.176
-# delOpens: 56.362
-# insOpens: 100.973
+# deletes: 188.952
+# inserts: 357.073
+# delOpens: 56.483
+# insOpens: 100.742
 # alignments: 5
-# mean delete size: 3.35245
-# mean insert size: 3.53734
-# matchProb: 0.989155
-# delOpenProb: 0.00374231
-# insOpenProb: 0.00670439
-# delExtendProb: 0.701711
-# insExtendProb: 0.717302
+# mean delete size: 3.34529
+# mean insert size: 3.54443
+# matchProb: 0.989162
+# delOpenProb: 0.00375038
+# insOpenProb: 0.0066891
+# delExtendProb: 0.701072
+# insExtendProb: 0.717867
 
-# substitution percent identity: 68.1403
+# substitution percent identity: 68.1444
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3081.6         634.732        445.81         423.98        
+# A 3081.6         633.732        445.81         423.98        
 # C 650.18         2986.2         169.39         816.1         
 # G 273.05         94.134         1561.4         71.9          
-# T 365.574        674.07         131.782        2531.4        
+# T 365.574        674.17         131.782        2531.4        
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206662       0.0425672      0.0298975      0.0284335     
-# C 0.0436032      0.200264       0.0113598      0.0547303     
-# G 0.0183116      0.00631293     0.104713       0.00482185    
-# T 0.0245166      0.0452053      0.00883773     0.169764      
+# A 0.206675       0.0425027      0.0298993      0.0284352     
+# C 0.0436058      0.200276       0.0113605      0.0547336     
+# G 0.0183127      0.00631331     0.104719       0.00482214    
+# T 0.0245181      0.0452147      0.00883826     0.169774      
 
-# delExistCost: 587
+# delExistCost: 586
 # insExistCost: 541
 # delExtendCost: 32
 # insExtendCost: 30
@@ -399,37 +399,37 @@
 # G    -71   -168    146   -180
 # T   -100    -45   -135     88
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.4821 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.494 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
 # aligned letter pairs: 14897.4
-# deletes: 189.355
-# inserts: 357.265
-# delOpens: 56.08
-# insOpens: 100.658
+# deletes: 190.558
+# inserts: 358.367
+# delOpens: 56.231
+# insOpens: 100.769
 # alignments: 5
-# mean delete size: 3.37652
-# mean insert size: 3.5493
-# matchProb: 0.989194
-# delOpenProb: 0.00372374
-# insOpenProb: 0.00668374
-# delExtendProb: 0.703837
-# insExtendProb: 0.718254
+# mean delete size: 3.38884
+# mean insert size: 3.55632
+# matchProb: 0.989177
+# delOpenProb: 0.0037337
+# insOpenProb: 0.00669099
+# delExtendProb: 0.704914
+# insExtendProb: 0.718811
 
-# substitution percent identity: 68.1453
+# substitution percent identity: 68.1458
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3081.6         633.731        445.81         423.98        
+# A 3081.6         633.732        445.81         423.98        
 # C 650.08         2986.2         169.39         816.1         
-# G 273.05         94.136         1561.4         71.9          
-# T 365.674        674.07         131.672        2531.4        
+# G 273.05         94.125         1561.4         71.9          
+# T 365.574        674.07         131.672        2531.4        
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206677       0.0425032      0.0298997      0.0284356     
-# C 0.0435997      0.200279       0.0113607      0.0547344     
-# G 0.018313       0.00631353     0.10472        0.0048222     
-# T 0.0245251      0.0452087      0.00883101     0.169776      
+# A 0.206679       0.0425036      0.0298999      0.0284358     
+# C 0.0436         0.200281       0.0113608      0.0547348     
+# G 0.0183131      0.00631284     0.104721       0.00482224    
+# T 0.0245186      0.045209       0.00883107     0.169778      
 
 # delExistCost: 588
 # insExistCost: 541
@@ -443,7 +443,7 @@
 # G    -71   -168    146   -180
 # T   -100    -45   -135     88
 
-# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.4704 -p- /home/rdallet/galaxy/tools/last/test-data/humdb /tmp/tmpow095T/tmp/tmpmqx0kk84
+# lastal -j7 -D1000000 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.4704 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpc7ashbco
 
 # aligned letter pairs: 14897.4
 # deletes: 188.951
--- a/test-data/last_train_E.txt	Fri Nov 13 09:05:49 2020 +0000
+++ b/test-data/last_train_E.txt	Wed Mar 24 22:36:50 2021 +0000
@@ -1,9 +1,9 @@
-# lastal version: 1021
+# lastal version: 1205
 # maximum percent identity: 100
 # scale of score parameters: 4.5512
 # scale used while training: 91.024
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -r5 -q5 -a15 -b3 -A15 -B3 ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -r5 -q5 -a15 -b3 -A15 -B3 /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14901
 # deletes: 694
@@ -47,7 +47,7 @@
 # G    -88   -164    145   -167
 # T   -112    -66   -127     90
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.4322 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.4322 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14771
 # deletes: 379.3
@@ -63,21 +63,21 @@
 # delExtendProb: 0.511732
 # insExtendProb: 0.566038
 
-# substitution percent identity: 70.0105
+# substitution percent identity: 70.0152
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3158           583.4          429.9          391.6         
+# A 3158           582.4          429.9          391.6         
 # C 589            3065           156.8          774           
 # G 252.2          88.64          1571           68.46         
 # T 342.6          630.1          131.09         2566          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.21341        0.0394248      0.0290516      0.0264634     
-# C 0.0398032      0.207126       0.0105962      0.0523051     
-# G 0.0170431      0.00599008     0.106165       0.00462637    
-# T 0.0231521      0.0425807      0.00885876     0.173404      
+# A 0.213425       0.0393599      0.0290536      0.0264652     
+# C 0.0398059      0.20714        0.0105969      0.0523086     
+# G 0.0170442      0.00599049     0.106172       0.00462668    
+# T 0.0231537      0.0425836      0.00885935     0.173416      
 
 # delExistCost: 406
 # insExistCost: 400
@@ -86,218 +86,218 @@
 
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
-# A     75    -79    -48   -102
+# A     76    -79    -48   -102
 # C    -78     72   -140    -41
 # G    -79   -174    146   -185
 # T   -107    -52   -136     89
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t90.8564 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.6863 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14881
-# deletes: 258.8
-# inserts: 446.1
-# delOpens: 112.91
-# insOpens: 168
+# deletes: 264.3
+# inserts: 451.9
+# delOpens: 116.18
+# insOpens: 171.3
 # alignments: 2
-# mean delete size: 2.29209
-# mean insert size: 2.65536
-# matchProb: 0.981278
-# delOpenProb: 0.00744548
-# insOpenProb: 0.0110782
-# delExtendProb: 0.563717
-# insExtendProb: 0.623403
+# mean delete size: 2.27492
+# mean insert size: 2.63806
+# matchProb: 0.980854
+# delOpenProb: 0.00765779
+# insOpenProb: 0.0112909
+# delExtendProb: 0.560424
+# insExtendProb: 0.620934
 
-# substitution percent identity: 68.9366
+# substitution percent identity: 68.9602
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3126           618.3          445            406.6         
-# C 622.8          3041           159.4          805.4         
-# G 265.2          91.16          1560           68.22         
-# T 357.3          660            133.33         2554          
+# A 3126           617.2          445            406.6         
+# C 621.9          3041           159.4          804.4         
+# G 264.2          91.07          1560           68.32         
+# T 357.3          658.9          133.32         2554          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.209606       0.0414585      0.0298383      0.0272635     
-# C 0.0417602      0.203906       0.0106882      0.054004      
-# G 0.0177823      0.0061125      0.104602       0.00457431    
-# T 0.0239578      0.0442546      0.0089401      0.171252      
+# A 0.209677       0.0413989      0.0298485      0.0272728     
+# C 0.0417142      0.203976       0.0106918      0.0539554     
+# G 0.0177213      0.00610855     0.104638       0.00458259    
+# T 0.023966       0.0441959      0.00894248     0.17131       
 
-# delExistCost: 470
-# insExistCost: 457
+# delExistCost: 466
+# insExistCost: 453
 # delExtendCost: 52
-# insExtendCost: 42
+# insExtendCost: 43
 
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
-# A     75    -73    -44    -99
+# A     75    -74    -44    -99
 # C    -73     71   -138    -37
-# G    -73   -171    147   -185
+# G    -74   -171    147   -185
 # T   -103    -48   -134     88
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.1831 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.1379 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14981
-# deletes: 214.8
-# inserts: 402.9
-# delOpens: 82.32
-# insOpens: 131.98
+# deletes: 217
+# inserts: 405.1
+# delOpens: 83.75
+# insOpens: 134.12
 # alignments: 2
-# mean delete size: 2.60933
-# mean insert size: 3.05274
-# matchProb: 0.985702
-# delOpenProb: 0.0054164
-# insOpenProb: 0.00868387
-# delExtendProb: 0.61676
-# insExtendProb: 0.672425
+# mean delete size: 2.59104
+# mean insert size: 3.02043
+# matchProb: 0.985471
+# delOpenProb: 0.00550919
+# insOpenProb: 0.0088226
+# delExtendProb: 0.614055
+# insExtendProb: 0.668921
 
-# substitution percent identity: 68.4035
+# substitution percent identity: 68.4369
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3114           635.9          450            416.3         
-# C 640.1          3019           164.9          816.3         
-# G 270.6          93.43          1560           69.38         
-# T 365.7          670            135.53         2543          
+# A 3114           634.8          449.1          416.3         
+# C 639            3019           163.9          815.3         
+# G 269.6          93.35          1560           69.28         
+# T 364.7          670            135.51         2543          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.208097       0.0424949      0.0300719      0.0278198     
-# C 0.0427756      0.201749       0.0110197      0.0545504     
-# G 0.0180832      0.00624359     0.104249       0.00463642    
-# T 0.0244384      0.0447737      0.00905699     0.16994       
+# A 0.208199       0.0424421      0.0300264      0.0278334     
+# C 0.0427229      0.201847       0.0109582      0.0545102     
+# G 0.0180252      0.00624129     0.1043         0.00463199    
+# T 0.0243835      0.0447956      0.00906007     0.170023      
 
-# delExistCost: 518
-# insExistCost: 498
+# delExistCost: 516
+# insExistCost: 495
 # delExtendCost: 44
 # insExtendCost: 36
 
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
-# A     74    -71    -43    -97
+# A     75    -71    -43    -97
 # C    -70     71   -135    -36
-# G    -71   -169    146   -183
+# G    -72   -169    147   -183
 # T   -101    -46   -133     88
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t90.7211 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.7798 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14981
-# deletes: 197.5
-# inserts: 386.4
-# delOpens: 68.51
-# insOpens: 115.71
+# deletes: 204.2
+# inserts: 393.3
+# delOpens: 71.01
+# insOpens: 119.07
 # alignments: 2
-# mean delete size: 2.88279
-# mean insert size: 3.33938
-# matchProb: 0.987657
-# delOpenProb: 0.00451668
-# insOpenProb: 0.00762845
-# delExtendProb: 0.653114
-# insExtendProb: 0.700543
+# mean delete size: 2.87565
+# mean insert size: 3.3031
+# matchProb: 0.987276
+# delOpenProb: 0.00467969
+# insOpenProb: 0.00784693
+# delExtendProb: 0.652253
+# insExtendProb: 0.697254
 
-# substitution percent identity: 68.1501
+# substitution percent identity: 68.1926
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3103           643.6          451.1          422.5         
-# C 649.8          3008           167            819.5         
-# G 272.7          94.59          1560           70.43         
-# T 369.8          675.4          136.61         2542          
+# A 3104           642.4          451.1          421.4         
+# C 647.7          3009           167            819.4         
+# G 271.7          94.39          1560           70.33         
+# T 368.8          674.3          136.58         2543          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.20706        0.0429467      0.0301014      0.0281929     
-# C 0.0433604      0.20072        0.0111437      0.0546843     
-# G 0.0181969      0.00631188     0.104097       0.00469971    
-# T 0.0246763      0.0450686      0.00911582     0.169625      
+# A 0.207194       0.0428807      0.0301113      0.0281288     
+# C 0.0432345      0.200853       0.0111474      0.0546956     
+# G 0.0181362      0.00630061     0.104131       0.00469458    
+# T 0.0246177      0.04501        0.00911682     0.169747      
 
-# delExistCost: 549
-# insExistCost: 522
+# delExistCost: 545
+# insExistCost: 517
 # delExtendCost: 39
-# insExtendCost: 32
+# insExtendCost: 33
 
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
-# A     74    -69    -43    -95
-# C    -68     70   -134    -35
+# A     74    -70    -43    -96
+# C    -69     70   -134    -35
 # G    -71   -168    146   -182
-# T   -100    -45   -132     88
+# T   -100    -46   -132     88
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t90.7441 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t90.3541 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14981
-# deletes: 192.1
-# inserts: 380.9
-# delOpens: 62.34
-# insOpens: 108.21
+# deletes: 194.4
+# inserts: 382.1
+# delOpens: 63.11
+# insOpens: 109.31
 # alignments: 2
-# mean delete size: 3.08149
-# mean insert size: 3.52001
-# matchProb: 0.988548
-# delOpenProb: 0.00411362
-# insOpenProb: 0.00714043
-# delExtendProb: 0.675482
-# insExtendProb: 0.71591
+# mean delete size: 3.08034
+# mean insert size: 3.49556
+# matchProb: 0.988426
+# delOpenProb: 0.00416391
+# insOpenProb: 0.00721213
+# delExtendProb: 0.67536
+# insExtendProb: 0.713923
 
-# substitution percent identity: 67.9883
+# substitution percent identity: 68.0193
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3093           648            451.1          425.7         
-# C 654.2          3008           169            821.5         
-# G 272.8          95.26          1550           71.05         
-# T 371.8          677.5          136.66         2532          
+# A 3093           646.9          451.1          424.6         
+# C 652.9          3008           168            821.5         
+# G 272.7          95.16          1550           70.95         
+# T 370.8          676.5          136.64         2532          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206509       0.0432647      0.0301184      0.0284225     
-# C 0.0436786      0.200834       0.0112835      0.0548487     
-# G 0.0182139      0.00636018     0.103488       0.00474376    
-# T 0.0248238      0.0452343      0.00912431     0.169053      
+# A 0.206603       0.0432109      0.0301321      0.028362      
+# C 0.0436117      0.200925       0.0112219      0.0548737     
+# G 0.0182155      0.00635639     0.103535       0.00473924    
+# T 0.0247683      0.0451881      0.00912713     0.16913       
 
-# delExistCost: 566
-# insExistCost: 534
+# delExistCost: 565
+# insExistCost: 533
 # delExtendCost: 36
 # insExtendCost: 30
 
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
-# A     74    -69    -42    -94
+# A     74    -69    -42    -95
 # C    -68     70   -133    -35
 # G    -70   -167    147   -181
 # T    -99    -45   -131     88
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.2473 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.1953 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14981
-# deletes: 194.5
-# inserts: 383.3
-# delOpens: 60.42
-# insOpens: 106.36
+# deletes: 195.6
+# inserts: 384.5
+# delOpens: 60.75
+# insOpens: 106.8
 # alignments: 2
-# mean delete size: 3.21913
-# mean insert size: 3.6038
-# matchProb: 0.988794
-# delOpenProb: 0.00398791
-# insOpenProb: 0.0070201
-# delExtendProb: 0.689357
-# insExtendProb: 0.722515
+# mean delete size: 3.21975
+# mean insert size: 3.60019
+# matchProb: 0.988744
+# delOpenProb: 0.00400949
+# insOpenProb: 0.00704878
+# delExtendProb: 0.689417
+# insExtendProb: 0.722237
 
-# substitution percent identity: 67.9593
+# substitution percent identity: 67.9748
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 3093           648.9          451.2          426.7         
-# C 655.2          3008           169            821.4         
-# G 273.8          95.46          1550           71.26         
-# T 372.8          678.6          136.64         2532          
+# A 3093           648.9          451.2          425.6         
+# C 655.1          3008           169            821.4         
+# G 272.8          95.36          1550           71.16         
+# T 372.8          677.6          136.64         2532          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206421       0.0433063      0.0301122      0.0284771     
-# C 0.0437268      0.200748       0.0112787      0.0548186     
-# G 0.0182729      0.00637081     0.103444       0.00475575    
-# T 0.0248799      0.0452884      0.00911908     0.168981      
+# A 0.206468       0.0433161      0.030119       0.0284102     
+# C 0.04373        0.200794       0.0112813      0.0548311     
+# G 0.0182103      0.00636558     0.103467       0.00475016    
+# T 0.0248856      0.045232       0.00912115     0.169019      
 
 # delExistCost: 575
 # insExistCost: 539
@@ -307,41 +307,41 @@
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
 # A     74    -69    -42    -94
-# C    -68     70   -132    -35
-# G    -70   -167    147   -180
+# C    -68     70   -133    -35
+# G    -70   -167    147   -181
 # T    -99    -45   -131     88
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.2311 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.1816 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14981
 # deletes: 196.9
 # inserts: 385.7
-# delOpens: 59.47
-# insOpens: 105.68
+# delOpens: 59.46
+# insOpens: 105.67
 # alignments: 2
-# mean delete size: 3.31091
-# mean insert size: 3.6497
-# matchProb: 0.9889
-# delOpenProb: 0.00392563
-# insOpenProb: 0.00697597
-# delExtendProb: 0.697969
-# insExtendProb: 0.726005
+# mean delete size: 3.31147
+# mean insert size: 3.65004
+# matchProb: 0.988902
+# delOpenProb: 0.00392498
+# insOpenProb: 0.00697532
+# delExtendProb: 0.698019
+# insExtendProb: 0.726031
 
-# substitution percent identity: 67.9572
+# substitution percent identity: 67.9622
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
 # A 3093           648.8          451.2          426.6         
-# C 655.1          3008           169.9          821.4         
+# C 655            3008           168.9          821.4         
 # G 273.8          95.45          1550           71.26         
-# T 372.7          678.6          136.63         2532          
+# T 372.7          678.6          136.62         2532          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206414       0.0432982      0.0301112      0.0284695     
-# C 0.0437187      0.200742       0.0113384      0.0548169     
-# G 0.0182723      0.00636994     0.103441       0.0047556     
-# T 0.0248725      0.045287       0.00911813     0.168975      
+# A 0.206429       0.0433015      0.0301135      0.0284716     
+# C 0.0437152      0.200756       0.0112725      0.0548209     
+# G 0.0182736      0.00637041     0.103448       0.00475595    
+# T 0.0248743      0.0452903      0.00911813     0.168988      
 
 # delExistCost: 580
 # insExistCost: 541
@@ -351,11 +351,11 @@
 # score matrix (query letters = columns, reference letters = rows):
 #        A      C      G      T
 # A     74    -69    -42    -94
-# C    -68     70   -132    -35
+# C    -68     70   -133    -35
 # G    -70   -167    147   -180
 # T    -99    -45   -131     88
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.1656 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.131 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14981
 # deletes: 197
@@ -371,21 +371,21 @@
 # delExtendProb: 0.701929
 # insExtendProb: 0.727405
 
-# substitution percent identity: 67.9308
+# substitution percent identity: 67.9358
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
 # A 3093           648.8          451.2          426.6         
-# C 656            2998           169.9          821.4         
-# G 273.8          95.55          1550           71.36         
+# C 656            2998           168.9          821.4         
+# G 273.8          95.45          1550           71.36         
 # T 372.7          678.6          136.62         2532          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206537       0.043324       0.0301292      0.0284865     
-# C 0.0438048      0.200193       0.0113452      0.0548495     
-# G 0.0182832      0.00638041     0.103502       0.00476511    
-# T 0.0248873      0.0453139      0.00912288     0.169076      
+# A 0.206552       0.0433272      0.0301314      0.0284886     
+# C 0.043808       0.200208       0.0112792      0.0548535     
+# G 0.0182845      0.0063742      0.10351        0.00476546    
+# T 0.0248891      0.0453173      0.00912355     0.169088      
 
 # delExistCost: 584
 # insExistCost: 542
@@ -397,9 +397,9 @@
 # A     74    -69    -42    -94
 # C    -68     70   -132    -35
 # G    -70   -166    146   -180
-# T    -99    -45   -132     88
+# T    -99    -45   -131     88
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t90.815 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t90.8423 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14981
 # deletes: 197
@@ -415,21 +415,153 @@
 # delExtendProb: 0.705736
 # insExtendProb: 0.728334
 
-# substitution percent identity: 67.93
+# substitution percent identity: 67.9254
 
 # count matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
 # A 3093           648.8          451.2          427.6         
 # C 656            2998           169.9          821.4         
 # G 273.8          95.75          1550           71.36         
-# T 372.7          678.6          135.61         2532          
+# T 372.7          678.6          136.62         2532          
+
+# probability matrix (query letters = columns, reference letters = rows):
+#   A              C              G              T             
+# A 0.20652        0.0433205      0.0301267      0.028551      
+# C 0.0438013      0.200177       0.0113443      0.0548451     
+# G 0.0182817      0.00639325     0.103494       0.00476473    
+# T 0.0248853      0.0453103      0.00912215     0.169062      
+
+# delExistCost: 586
+# insExistCost: 543
+# delExtendCost: 32
+# insExtendCost: 29
+
+# score matrix (query letters = columns, reference letters = rows):
+#        A      C      G      T
+# A     74    -69    -43    -94
+# C    -68     70   -132    -35
+# G    -70   -166    146   -180
+# T    -99    -45   -132     88
+
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t90.682 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
+
+# aligned letter pairs: 14981
+# deletes: 194.8
+# inserts: 383.5
+# delOpens: 57.43
+# insOpens: 103.95
+# alignments: 2
+# mean delete size: 3.39196
+# mean insert size: 3.68927
+# matchProb: 0.989147
+# delOpenProb: 0.00379192
+# insOpenProb: 0.00686348
+# delExtendProb: 0.705185
+# insExtendProb: 0.728944
+
+# substitution percent identity: 67.9249
+
+# count matrix (query letters = columns, reference letters = rows):
+#   A              C              G              T             
+# A 3093           649.9          451.1          427.6         
+# C 656            2998           169.9          821.4         
+# G 273.8          95.75          1550           71.46         
+# T 372.7          678.6          135.62         2532          
 
 # probability matrix (query letters = columns, reference letters = rows):
 #   A              C              G              T             
-# A 0.206534       0.0433235      0.0301288      0.0285529     
-# C 0.0438042      0.200191       0.011345       0.0548488     
-# G 0.0182829      0.00639368     0.103501       0.00476505    
-# T 0.024887       0.0453133      0.00905532     0.169074      
+# A 0.206519       0.0433937      0.0301199      0.0285508     
+# C 0.043801       0.200176       0.0113442      0.0548447     
+# G 0.0182816      0.00639321     0.103493       0.00477137    
+# T 0.0248851      0.04531        0.00905532     0.169061      
+
+# delExistCost: 587
+# insExistCost: 543
+# delExtendCost: 32
+# insExtendCost: 29
+
+# score matrix (query letters = columns, reference letters = rows):
+#        A      C      G      T
+# A     74    -68    -42    -94
+# C    -68     70   -132    -35
+# G    -70   -166    147   -180
+# T    -99    -45   -132     88
+
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.2049 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
+
+# aligned letter pairs: 14981
+# deletes: 195.9
+# inserts: 384.7
+# delOpens: 57.66
+# insOpens: 104.5
+# alignments: 2
+# mean delete size: 3.3975
+# mean insert size: 3.68134
+# matchProb: 0.989096
+# delOpenProb: 0.00380691
+# insOpenProb: 0.00689944
+# delExtendProb: 0.705666
+# insExtendProb: 0.72836
+
+# substitution percent identity: 67.9195
+
+# count matrix (query letters = columns, reference letters = rows):
+#   A              C              G              T             
+# A 3093           649.9          451.2          427.6         
+# C 656.1          2998           169.9          821.4         
+# G 273.8          95.74          1550           71.46         
+# T 373.7          678.6          135.62         2532          
+
+# probability matrix (query letters = columns, reference letters = rows):
+#   A              C              G              T             
+# A 0.206503       0.0433902      0.0301241      0.0285485     
+# C 0.0438042      0.20016        0.0113433      0.0548404     
+# G 0.0182801      0.00639203     0.103485       0.00477099    
+# T 0.0249499      0.0453064      0.0090546      0.169048      
+
+# delExistCost: 586
+# insExistCost: 543
+# delExtendCost: 32
+# insExtendCost: 29
+
+# score matrix (query letters = columns, reference letters = rows):
+#        A      C      G      T
+# A     74    -68    -42    -94
+# C    -68     70   -132    -35
+# G    -70   -166    147   -180
+# T    -99    -45   -132     88
+
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.2167 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
+
+# aligned letter pairs: 14981
+# deletes: 196
+# inserts: 384.7
+# delOpens: 57.87
+# insOpens: 104.51
+# alignments: 2
+# mean delete size: 3.3869
+# mean insert size: 3.68099
+# matchProb: 0.989081
+# delOpenProb: 0.00382071
+# insOpenProb: 0.0069
+# delExtendProb: 0.704745
+# insExtendProb: 0.728334
+
+# substitution percent identity: 67.9245
+
+# count matrix (query letters = columns, reference letters = rows):
+#   A              C              G              T             
+# A 3093           649.9          451.2          427.6         
+# C 656.1          2998           169.9          821.4         
+# G 273.8          95.64          1550           71.46         
+# T 372.7          678.6          135.62         2532          
+
+# probability matrix (query letters = columns, reference letters = rows):
+#   A              C              G              T             
+# A 0.206518       0.0433934      0.0301264      0.0285506     
+# C 0.0438074      0.200175       0.0113441      0.0548444     
+# G 0.0182815      0.00638583     0.103493       0.00477134    
+# T 0.024885       0.0453097      0.00905527     0.16906       
 
 # delExistCost: 586
 # insExistCost: 543
@@ -443,7 +575,7 @@
 # G    -70   -166    147   -180
 # T    -99    -45   -132     88
 
-# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.0834 -p- ref_db /tmp/tmpikDSIL/tmp/tmpDOHHwB
+# lastal -j7 -D1000000 -E10 -s1 -S1 -T0 -m10 -k1 -P1 -X0 -Q0 -t91.0834 -p- /home/berntm/.planemo/planemo_tmp_y1t9frxe/test-data/humdb /tmp/tmpw04gddt3
 
 # aligned letter pairs: 14981
 # deletes: 196