Mercurial > repos > iuc > meme_fimo

annotate fimo_wrapper.py @ 9:40b8472e91ca draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit bcfc8451fa622a6bf3aa31fa1e308c394feefb38
author iuc
date Mon, 14 Nov 2016 13:17:22 -0500
parents cdcc4bb60bc3
children c470b36b592d
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fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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1 #!/usr/bin/env python
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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2 import argparse
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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3 import os
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4 import shutil
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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5 import string
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6 import subprocess
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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7 import sys
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8 import tempfile
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9
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10 BUFFSIZE = 1048576
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11 # Translation table for reverse Complement, with ambiguity codes.
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12 DNA_COMPLEMENT = string.maketrans("ACGTRYKMBDHVacgtrykmbdhv", "TGCAYRMKVHDBtgcayrmkvhdb")
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14
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15 def get_stderr(tmp_stderr):
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16 tmp_stderr.seek(0)
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17 stderr = ''
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18 try:
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19 while True:
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20 stderr += tmp_stderr.read(BUFFSIZE)
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21 if not stderr or len(stderr) % BUFFSIZE != 0:
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22 break
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23 except OverflowError:
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24 pass
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25 return stderr
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26
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28 def reverse(sequence):
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29 # Reverse sequence string.
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30 return sequence[::-1]
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33 def dna_complement(sequence):
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34 # Complement DNA sequence string.
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35 return sequence.translate(DNA_COMPLEMENT)
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38 def dna_reverse_complement(sequence):
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39 # Returns the reverse complement of the sequence.
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40 sequence = reverse(sequence)
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41 return dna_complement(sequence)
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44 def stop_err(msg):
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45 sys.stderr.write(msg)
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46 sys.exit(1)
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47
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48
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49 parser = argparse.ArgumentParser()
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50 parser.add_argument('--input_motifs', dest='input_motifs', help='MEME output formatted files for input to fimo')
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51 parser.add_argument('--input_fasta', dest='input_fasta', help='Fassta sequence file')
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52 parser.add_argument('--options_type', dest='options_type', help='Basic or Advance options')
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53 parser.add_argument('--input_psp', dest='input_psp', default=None, help='File containing position specific priors')
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54 parser.add_argument('--input_prior_dist', dest='input_prior_dist', default=None, help='File containing binned distribution of priors')
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55 parser.add_argument('--alpha', dest='alpha', type=float, default=1.0, help='The alpha parameter for calculating position specific priors')
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56 parser.add_argument('--bgfile', dest='bgfile', default=None, help='Background file type, used only if not "default"')
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57 parser.add_argument('--max_strand', action='store_true', help='If matches on both strands at a given position satisfy the output threshold, only report the match for the strand with the higher score')
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58 parser.add_argument('--max_stored_scores', dest='max_stored_scores', type=int, help='Maximum score count to store')
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59 parser.add_argument('--motif', dest='motifs', action='append', default=[], help='Specify motif by id')
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60 parser.add_argument('--output_separate_motifs', dest='output_separate_motifs', default='no', help='Output one dataset per motif')
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61 parser.add_argument('--motif_pseudo', dest='motif_pseudo', type=float, default=0.1, help='Pseudocount to add to counts in motif matrix')
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62 parser.add_argument('--no_qvalue', action='store_true', help='Do not compute a q-value for each p-value')
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63 parser.add_argument('--norc', action='store_true', help='Do not score the reverse complement DNA strand')
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64 parser.add_argument('--output_path', dest='output_path', help='Output files directory')
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65 parser.add_argument('--parse_genomic_coord', dest='parse_genomic_coord', default='no', help='Check each sequence header for UCSC style genomic coordinates')
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66 parser.add_argument('--remove_duplicate_coords', dest='remove_duplicate_coords', default='no', help='Remove duplicate entries in unique GFF coordinates')
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67 parser.add_argument('--qv_thresh', action='store_true', help='Use q-values for the output threshold')
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68 parser.add_argument('--thresh', dest='thresh', type=float, help='p-value threshold')
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69 parser.add_argument('--gff_output', dest='gff_output', help='Gff output file')
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70 parser.add_argument('--html_output', dest='html_output', help='HTML output file')
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71 parser.add_argument('--interval_output', dest='interval_output', help='Interval output file')
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72 parser.add_argument('--txt_output', dest='txt_output', help='Text output file')
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73 parser.add_argument('--xml_output', dest='xml_output', help='XML output file')
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74 args = parser.parse_args()
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75
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76 fimo_cmd_list = ['fimo']
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77 if args.options_type == 'advanced':
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78 fimo_cmd_list.append('--alpha %4f' % args.alpha)
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79 if args.bgfile is not None:
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80 fimo_cmd_list.append('--bgfile "%s"' % args.bgfile)
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81 if args.max_strand:
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82 fimo_cmd_list.append('--max-strand')
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83 fimo_cmd_list.append('--max-stored-scores %d' % args.max_stored_scores)
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84 if len(args.motifs) > 0:
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85 for motif in args.motifs:
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86 fimo_cmd_list.append('--motif "%s"' % motif)
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87 fimo_cmd_list.append('--motif-pseudo %4f' % args.motif_pseudo)
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88 if args.no_qvalue:
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89 fimo_cmd_list.append('--no-qvalue')
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90 if args.norc:
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91 fimo_cmd_list.append('--norc')
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92 if args.parse_genomic_coord == 'yes':
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93 fimo_cmd_list.append('--parse-genomic-coord')
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94 if args.qv_thresh:
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95 fimo_cmd_list.append('--qv-thresh')
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96 fimo_cmd_list.append('--thresh %4f' % args.thresh)
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97 if args.input_psp is not None:
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98 fimo_cmd_list.append('--psp "%s"' % args.input_psp)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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99 if args.input_prior_dist is not None:
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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100 fimo_cmd_list.append('--prior-dist "%s"' % args.input_prior_dist)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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101 fimo_cmd_list.append('--o "%s"' % (args.output_path))
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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102 fimo_cmd_list.append('--verbosity 1')
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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103 fimo_cmd_list.append(args.input_motifs)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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104 fimo_cmd_list.append(args.input_fasta)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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105
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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106 fimo_cmd = ' '.join(fimo_cmd_list)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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107
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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108 try:
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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109 tmp_stderr = tempfile.NamedTemporaryFile()
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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110 proc = subprocess.Popen(args=fimo_cmd, shell=True, stderr=tmp_stderr)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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111 returncode = proc.wait()
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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112 if returncode != 0:
5
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113 stderr = get_stderr(tmp_stderr)
0
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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114 stop_err(stderr)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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115 except Exception, e:
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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116 stop_err('Error running FIMO:\n%s' % str(e))
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117
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118 shutil.move(os.path.join(args.output_path, 'fimo.txt'), args.txt_output)
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119
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120 gff_file = os.path.join(args.output_path, 'fimo.gff')
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121 if args.remove_duplicate_coords == 'yes':
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122 tmp_stderr = tempfile.NamedTemporaryFile()
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123 # Identify and eliminating identical motif occurrences. These
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124 # are identical if the combination of chrom, start, end and
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125 # motif id are identical.
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126 cmd = 'sort -k1,1 -k4,4n -k5,5n -k9.1,9.6 -u -o %s %s' % (gff_file, gff_file)
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127 proc = subprocess.Popen(args=cmd, stderr=tmp_stderr, shell=True)
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128 returncode = proc.wait()
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129 if returncode != 0:
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130 stderr = get_stderr(tmp_stderr)
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131 stop_err(stderr)
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132 # Sort GFF output by a combination of chrom, score, start.
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133 cmd = 'sort -k1,1 -k4,4n -k6,6n -o %s %s' % (gff_file, gff_file)
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134 proc = subprocess.Popen(args=cmd, stderr=tmp_stderr, shell=True)
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135 returncode = proc.wait()
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136 if returncode != 0:
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137 stderr = get_stderr(tmp_stderr)
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138 stop_err(stderr)
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139 if args.output_separate_motifs == 'yes':
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140 # Create the collection output directory.
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141 collection_path = (os.path.join(os.getcwd(), 'output'))
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142 # Keep track of motif occurrences.
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143 header_line = None
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144 motif_ids = []
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145 file_handles = []
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146 for line in open(gff_file, 'r'):
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147 if line.startswith('#'):
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148 if header_line is None:
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149 header_line = line
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150 continue
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151 items = line.split('\t')
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152 attribute = items[8]
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153 attributes = attribute.split(';')
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154 name = attributes[0]
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155 motif_id = name.split('=')[1]
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156 file_name = os.path.join(collection_path, 'MOTIF%s.gff' % motif_id)
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157 if motif_id in motif_ids:
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158 i = motif_ids.index(motif_id)
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159 fh = file_handles[i]
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160 fh.write(line)
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161 else:
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162 fh = open(file_name, 'wb')
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163 if header_line is not None:
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164 fh.write(header_line)
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165 fh.write(line)
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166 motif_ids.append(motif_id)
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167 file_handles.append(fh)
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168 for file_handle in file_handles:
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169 file_handle.close()
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170 else:
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171 shutil.move(gff_file, args.gff_output)
0
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172 shutil.move(os.path.join(args.output_path, 'fimo.xml'), args.xml_output)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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173 shutil.move(os.path.join(args.output_path, 'fimo.html'), args.html_output)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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174
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175 out_file = open(args.interval_output, 'wb')
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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176 out_file.write("#%s\n" % "\t".join(("chr", "start", "end", "pattern name", "score", "strand", "matched sequence", "p-value", "q-value")))
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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177 for line in open(args.txt_output):
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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178 if line.startswith('#'):
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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179 continue
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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180 fields = line.rstrip("\n\r").split("\t")
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181 start, end = int(fields[2]), int(fields[3])
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182 sequence = fields[7]
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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183 if start > end:
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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184 # Flip start and end and set strand.
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185 start, end = end, start
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186 strand = "-"
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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187 # We want sequences relative to strand; FIMO always provides + stranded sequence.
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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188 sequence = dna_reverse_complement(sequence)
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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189 else:
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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190 strand = "+"
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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191 # Make 0-based start position.
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
iuc
parents:
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192 start -= 1
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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193 out_file.write("%s\n" % "\t".join([fields[1], str(start), str(end), fields[0], fields[4], strand, sequence, fields[5], fields[6]]))
fd522a964017 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
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194 out_file.close()