Mercurial > repos > iuc > obi_illumina_pairend
changeset 3:83fbdf93d51e draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/obitools commit 5d3c7a7b3f7d687bb03ef7993ddf1a6507d655bd"
| author | iuc |
|---|---|
| date | Mon, 10 May 2021 19:36:09 +0000 |
| parents | 94f412098ac8 |
| children | 8cb6bd511879 |
| files | illuminapairedend.xml macros.xml test-data/illuminapairedend.output.fastq.gz test-data/output_ngsfilter_error_3.fastq test-data/output_ngsfilter_error_3.fastq.gz test-data/output_ngsfilter_unidentified.fastq test-data/output_obiannotate.fasta.gz test-data/output_obiclean_advanced.fasta.gz test-data/output_obigrep_predicat.fasta.gz test-data/output_obisort.fastq test-data/output_obiuniq.fasta.gz test-data/output_obiuniq_family.fasta.gz test-data/wolf_small.F.fastq.gz test-data/wolf_small.R.fastq.gz |
| diffstat | 14 files changed, 437 insertions(+), 249 deletions(-) [+] |
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--- a/illuminapairedend.xml Thu Nov 28 15:53:01 2019 -0500 +++ b/illuminapairedend.xml Mon May 10 19:36:09 2021 +0000 @@ -1,70 +1,95 @@ -<tool id="obi_illumina_pairend" name="Illuminapairedend - Assembling pair-end reads" version="@TOOL_VERSION@"> - <description>Construct consensus reads from Illumina pair-end reads</description> - <macros> - <import>macros.xml</import> - </macros> - <expand macro="requirements"/> - <expand macro="stdio"/> - <command> - - <![CDATA[ - illuminapairedend - - --score-min='$score' - -r '$inputfastq3p' - '$inputfastq5p' > '$output' - - ]]> - - </command> - - <inputs> - <param name="inputfastq3p" type="data" format="fastq" label="Read from file" help="file of 3p (1:) Illumina pair-end reads to assemble in sanger fastq nucleic format (standard fastq)" /> - <param name="inputfastq5p" type="data" format="fastq" label="Read from file" help="file of 5p (2:) Illumina pair-end reads to assemble in sanger fastq nucleic format (standard fastq)" /> - <param name="score" type="float" value="40.0" label="minimum score for keeping aligment"/> - </inputs> - <outputs> - <data format="fastq" name="output" label="${tool.name} on ${on_string}: assembly results" /> - </outputs> - - <tests> - <test> - <param name="inputfastq3p" value="wolf_small.F.fastq" /> - <param name="inputfastq5p" value="wolf_small.R.fastq" /> - <param name="score" value="40.0" /> - <output name="output" file="illuminapairedend.output.fastq" ftype="fastq"/> - </test> - </tests> - - <help><![CDATA[ - -.. class:: warning - -**Warning:** -Sequence records corresponding to the same read pair must be in the same order in the two files - --------- - -.. class:: infomark - -**What it does** - -illuminapairedend aims at aligning the two reads of a pair-end library sequenced using an Illumina platform : - -\* If the two reads overlap, it returns the consensus sequence together with its quality -\* Otherwise, it concatenates sequence merging the forward read and the reversed-complemented reverse read. - -The program uses as input one or two fastq sequences reads files. - -\* If two files are used one of them must be specified using the -r option. Sequence records corresponding to the same read pair must be in the same order in the two files. -\* If just one file is provided, sequence records are supposed to be all of the same length. The first half of th e sequence is used as forward read, the second half is used as the reverse read. - -illuminapairedend align the forward sequence record with the reverse complement of the reverse sequence record. The alignment algorithm takes into account the base qualities. - -@OBITOOLS_LINK@ - -]]> - </help> - <expand macro="citation" /> - -</tool> +<tool id="obi_illumina_pairend" name="Illuminapairedend" version="@TOOL_VERSION@" profile="@PROFILE@"> + <description>Construct consensus reads from Illumina pair-end reads</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <expand macro="stdio"/> + <command> + <![CDATA[ + #if $inputfastq3p.ext.endswith(".gz") + gunzip -c '$inputfastq3p' > fastq3p.fastq && + gunzip -c '$inputfastq5p' > fastq5p.fastq && + #else + ln -s '$inputfastq3p' fastq3p.fastq && + ln -s '$inputfastq5p' fastq5p.fastq && + #end if + + illuminapairedend + ##--index-file= + #if $inputfastq3p.ext.startswith("fastqsolexa") + ##input file is in fastq nucleic format produced by solexa sequencer + --solexa + #else if $inputfastq3p.ext.startswith("fastqillumina") + ##input file is in fastq nucleic format produced by solexa sequencer + --illumina + #else + ## input file is in sanger fastq nucleic format (standard fastq) + --sanger + #end if + --without-progress-bar + --score-min='$score' + -r fastq3p.fastq + fastq5p.fastq + #if $inputfastq3p.ext.endswith(".gz") + | gzip -c + #end if + > '$output' + ]]> + </command> + <inputs> + <param name="inputfastq3p" type="data" format="fastq,fastq.gz" label="Read from file" help="file of 3p (1:) Illumina pair-end reads to assemble in sanger fastq nucleic format (standard fastq)" /> + <param name="inputfastq5p" type="data" format="fastq,fastq.gz" label="Read from file" help="file of 5p (2:) Illumina pair-end reads to assemble in sanger fastq nucleic format (standard fastq)" /> + <param name="score" type="float" value="40.0" label="minimum score for keeping aligment"/> + </inputs> + <outputs> + <data name="output" format_source="inputfastq3p" label="${tool.name} on ${on_string}: assembly results" /> + </outputs> + + <tests> + <test> + <param name="inputfastq3p" value="wolf_small.F.fastq" ftype="fastqsanger" /> + <param name="inputfastq5p" value="wolf_small.R.fastq" ftype="fastqsanger" /> + <param name="score" value="40.0" /> + <output name="output" file="illuminapairedend.output.fastq" ftype="fastqsanger" /> + </test> + <test> + <param name="inputfastq3p" value="wolf_small.F.fastq.gz" ftype="fastqsanger.gz" /> + <param name="inputfastq5p" value="wolf_small.R.fastq.gz" ftype="fastqsanger.gz" /> + <param name="score" value="40.0" /> + <output name="output" file="illuminapairedend.output.fastq.gz" ftype="fastqsanger.gz" decompress="true"/> + </test> + </tests> + + <help><![CDATA[ + +.. class:: warning + +**Warning:** +Sequence records corresponding to the same read pair must be in the same order in the two files + +-------- + +.. class:: infomark + +**What it does** + +illuminapairedend aims at aligning the two reads of a pair-end library sequenced using an Illumina platform : + +\* If the two reads overlap, it returns the consensus sequence together with its quality +\* Otherwise, it concatenates sequence merging the forward read and the reversed-complemented reverse read. + +The program uses as input one or two fastq sequences reads files. + +\* If two files are used one of them must be specified using the -r option. Sequence records corresponding to the same read pair must be in the same order in the two files. +\* If just one file is provided, sequence records are supposed to be all of the same length. The first half of th e sequence is used as forward read, the second half is used as the reverse read. + +illuminapairedend align the forward sequence record with the reverse complement of the reverse sequence record. The alignment algorithm takes into account the base qualities. + +@OBITOOLS_LINK@ + +]]> + </help> + <expand macro="citation" /> + +</tool>
--- a/macros.xml Thu Nov 28 15:53:01 2019 -0500 +++ b/macros.xml Mon May 10 19:36:09 2021 +0000 @@ -6,7 +6,8 @@ </requirements> </xml> - <token name="@TOOL_VERSION@">1.2.11</token> + <token name="@TOOL_VERSION@">1.2.13</token> + <token name="@PROFILE@">21.01</token> <xml name="stdio"> <stdio> @@ -14,6 +15,80 @@ </stdio> </xml> + <token name="@INPUT_FORMATS@">fastqsanger,fastqsanger.gz,fastqsolexa,fastqsolexa.gz,fasta,fasta.gz</token> + <token name="@GUNZIP_INPUT@"><![CDATA[ + #if $input.ext.endswith(".gz") + gunzip -c '$input' > input && + #else + ln -s '$input' input && + #end if + ]]></token> + <token name="@GZIP_OUTPUT@"><![CDATA[ + #if $input.ext.endswith(".gz") + | gzip -c + #end if + ]]></token> + + <!-- generate galaxy.json to determine output format + - by default same as input + - if out_format can be specified + - fasta/fastq depending on $out_format (if != "") + - or fasta if the tool does not support choosing the output format, e.g. obiuniq which always outputs fasta + - gz if $input is zipped + + A list of outputs to include in the json can be given by + setting a variable $outputs = [...] listing pairs of output names and the actual output + (defaults to [("output", $output)]) + --> + <token name="@GENERATE_GALAXY_JSON@"><![CDATA[ + #import json + + #try: + #silent $outputs[0] + #except + #set outputs = [("output", $output)] + #end try + + #if $input.ext.startswith("fastq") + #set ext = "fastqsanger" + #else if $input.ext.startswith("fasta") + #set ext = "fasta" + #end if + + #try + #if $out_format == "fasta" + #set ext = "fasta" + #else if $out_format == "fastq" + #set ext = "fastqsanger" + #end if + #except + #set ext = "fasta" + #end try + + #if $input.ext.endswith(".gz") + #set ext = ext + ".gz" + #end if + + #set gxy_json = {} + #for oname, o in $outputs + #silent gxy_json[oname] = {"ext": ext} + #end for + && echo '${json.dumps(gxy_json)}' >> galaxy.json + ]]></token> + + <token name="@OUT_FORMAT@"><![CDATA[ + #if $out_format + --${out_format}-output + #end if + ]]></token> + + <xml name="out_format_macro"> + <param name="out_format" type="select" optional="true" label="Output data type" help="For FASTA/Q the the default output type is the same as the input type"> + <option value="fasta">fasta</option> + <option value="fastq">fastq</option> + </param> + </xml> + <token name="@OBITOOLS_LINK@"> <![CDATA[ -------- @@ -105,15 +180,43 @@ <option value="taxid">taxid</option> </xml> - <xml name="inputtype"> - <option value="--genbank">genbank</option> - <option value="--embl">embl</option> - <option value="--sanger">sanger</option> - <option value="--solexa">solexa</option> - <option value="--ecopcr">ecopcr</option> - <option value="--ecopcrdb">ecopcrdb</option> - <option value="--fasta" selected="true">fasta</option> + <xml name="input_format_options_macro"> + <section name="input_format_options" title="Input format options" expanded="false"> + <param name="options_inputtype" type="select" optional="true" label="Specify the input datatype" help="default: determine automatically (should only be necessay for non FASTA/FASTQ datasets)"> + <option value="--genbank">genbank</option> + <option value="--embl">embl</option> + <option value="--sanger">sanger</option> + <option value="--solexa">solexa</option> + <option value="--ecopcr">ecopcr</option> + <option value="--ecopcrdb">ecopcrdb</option> + <option value="--fasta">fasta (including obitools fasta extentions)</option> + <option value="--raw-fasta">raw fasta (more tolerant format variant)</option> + </param> + <param name="options_seqtype" type="select" optional="true" label="Specify the sequence datatype" > + <option value="--nuc">nucleic</option> + <option value="--prot">protein</option> + </param> + </section> </xml> + + <token name="@INPUT_FORMAT@"><![CDATA[ + #if $input_format_options.options_inputtype + $input_format_options.options_inputtype + #else + #if $input.ext.startswith("fasta") + --fasta + #else if $input.ext.startswith("fastqsolexa") + ## input file is in fastq nucleic format produced by solexa sequencer + --solexa + #else + ## input file is in sanger fastq nucleic format (standard fastq) + --sanger + #end if + #end if + #if $input_format_options.options_seqtype + $input_format_options.options_seqtype + #end if + ]]></token> <xml name="sanitizer"> <sanitizer invalid_char="test">
--- a/test-data/output_obisort.fastq Thu Nov 28 15:53:01 2019 -0500 +++ b/test-data/output_obisort.fastq Mon May 10 19:36:09 2021 +0000 @@ -1,172 +1,232 @@ ->HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP count=64; merged_sample={'26a_F040644': 64}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=99; obiclean_headcount=1; seq_rank=5; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'h'}; obiclean_head=True; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca -gcctgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB count=61; merged_sample={'29a_F260619': 30, '15a_F730814': 31}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=2; seq_rank=4; obiclean_samplecount=2; obiclean_status={'29a_F260619': 'h', '15a_F730814': 'h'}; obiclean_head=True; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB count=46; merged_sample={'13a_F730603': 46}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=1; seq_rank=8; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'h'}; obiclean_head=True; -ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB count=7; merged_sample={'29a_F260619': 7}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=4; obiclean_headcount=0; seq_rank=16; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; +@HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP count=64; merged_sample={'26a_F040644': 64}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=99; obiclean_headcount=1; seq_rank=5; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'h'}; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB count=61; merged_sample={'29a_F260619': 30, '15a_F730814': 31}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=2; seq_rank=4; obiclean_samplecount=2; obiclean_status={'29a_F260619': 'h', '15a_F730814': 'h'}; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB count=46; merged_sample={'13a_F730603': 46}; obiclean_singletoncount=0; obiclean_internalcount=0; seq_length=100; obiclean_headcount=1; seq_rank=8; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'h'}; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB count=7; merged_sample={'29a_F260619': 7}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=4; obiclean_headcount=0; seq_rank=16; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; tttt ->HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP count=3; merged_sample={'29a_F260619': 3}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=6; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; -ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaac -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB count=2; merged_sample={'29a_F260619': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=7; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; -ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP count=2; merged_sample={'29a_F260619': 1, '15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=2; seq_length=100; obiclean_headcount=0; seq_rank=13; obiclean_samplecount=2; obiclean_status={'29a_F260619': 'i', '15a_F730814': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB count=2; merged_sample={'26a_F040644': 2}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=15; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaaca -gcctgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB count=2; merged_sample={'15a_F730814': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=20; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccttt ->HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=1; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; -ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaat -agctcaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=2; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=3; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaag -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=9; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttatcccctt ->HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=10; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; -ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=11; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; -ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaata -gcttaaaactcaaaggacttggcggtgctttatatccct ->HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=12; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; -ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata -gcttaaaactcaaagaactgggcggtgctttatatcccg ->HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=14; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; -ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaaca -ggctgaaactcaaaggacttggcggtggtttacatccct ->HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=17; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca -gcctgaaactcacaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=18; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat -agcataaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=19; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaat -agcttaaaactcaaaggacttggcggtgctttatacgctt ->HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=21; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; -ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctg ->HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=22; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=23; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaaca -gcctgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=24; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=25; obiclean_samplecount=1; obiclean_status={'13a_F730603': 's'}; obiclean_head=True; -ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaat -agctcaaaactcaaagaacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=26; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=27; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=28; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; -ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=29; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; -ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=30; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaaca -gcctgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=31; obiclean_samplecount=1; obiclean_status={'13a_F730603': 's'}; obiclean_head=True; -ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcact -agcttacaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=32; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca -gcctgaaactcaaagggcttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=33; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca -gcctggaacgcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=34; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca -gcctgaaactcaaagcactcggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=35; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=36; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca -gcctgaaactcaaaggacttggcggtgctttatatccct ->HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=37; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=5; obiclean_headcount=0; seq_rank=38; obiclean_samplecount=1; obiclean_status={'13a_F730603': 's'}; obiclean_head=True; ++ +IIII +@HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP count=3; merged_sample={'29a_F260619': 3}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=6; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; +ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB count=2; merged_sample={'29a_F260619': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=7; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP count=2; merged_sample={'29a_F260619': 1, '15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=2; seq_length=100; obiclean_headcount=0; seq_rank=13; obiclean_samplecount=2; obiclean_status={'29a_F260619': 'i', '15a_F730814': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB count=2; merged_sample={'26a_F040644': 2}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=15; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB count=2; merged_sample={'15a_F730814': 2}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=20; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccttt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=1; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaatagctcaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=2; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=3; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaagagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=9; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatcccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=10; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=11; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=12; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaagaactgggcggtgctttatatcccg ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=14; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; +ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaacaggctgaaactcaaaggacttggcggtggtttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=17; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcacaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=18; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcataaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=19; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaatagcttaaaactcaaaggacttggcggtgctttatacgctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=21; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; +ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctg ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=22; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=23; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=24; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=25; obiclean_samplecount=1; obiclean_status={'13a_F730603': 's'}; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagctcaaaactcaaagaacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=26; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=27; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=28; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; +ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=29; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; +ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=30; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=31; obiclean_samplecount=1; obiclean_status={'13a_F730603': 's'}; obiclean_head=True; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcactagcttacaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=32; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaagggcttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=33; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctggaacgcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=34; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaagcactcggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=35; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=36; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttatatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=37; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=5; obiclean_headcount=0; seq_rank=38; obiclean_samplecount=1; obiclean_status={'13a_F730603': 's'}; obiclean_head=True; caata ->HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=39; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; -ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctc ->HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=40; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; -ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=41; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaaca -gcctgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=42; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; -ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=43; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; -ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaac -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=44; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; -ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=45; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=46; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaata -gcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=47; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaaca -gcctgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=48; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca -gcctgaaactcaaaggacttggcggtgctttacacccct ->HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=49; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; -ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaaca -gcatgaaactcaaagaactgggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=98; obiclean_headcount=0; seq_rank=50; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaaca -gccgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=51; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=52; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaaca -gcctgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=53; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; -ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaata -gcctgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=54; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; -ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=55; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaaca -gcctgaaactcaaaggacttggcggtgctttacatccct ->HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=56; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; -ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=57; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; -ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaat -agcttaaaactcaaaggacttggcggtgctttataccctt ->HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=58; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; -ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaaca -gcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIII +@HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=39; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctc ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=40; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=41; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=42; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; +ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=43; obiclean_samplecount=1; obiclean_status={'29a_F260619': 's'}; obiclean_head=True; +ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=44; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; +ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=45; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=46; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=47; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=48; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacacccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=49; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; +ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaacagcatgaaactcaaagaactgggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=98; obiclean_headcount=0; seq_rank=50; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagccgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'29a_F260619': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=51; obiclean_samplecount=1; obiclean_status={'29a_F260619': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=52; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=99; obiclean_headcount=0; seq_rank=53; obiclean_samplecount=1; obiclean_status={'26a_F040644': 's'}; obiclean_head=True; +ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=54; obiclean_samplecount=1; obiclean_status={'15a_F730814': 'i'}; obiclean_head=False; +ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=55; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB count=1; merged_sample={'15a_F730814': 1}; obiclean_singletoncount=1; obiclean_internalcount=0; seq_length=100; obiclean_headcount=0; seq_rank=56; obiclean_samplecount=1; obiclean_status={'15a_F730814': 's'}; obiclean_head=True; +ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB count=1; merged_sample={'13a_F730603': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=100; obiclean_headcount=0; seq_rank=57; obiclean_samplecount=1; obiclean_status={'13a_F730603': 'i'}; obiclean_head=False; +ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP count=1; merged_sample={'26a_F040644': 1}; obiclean_singletoncount=0; obiclean_internalcount=1; seq_length=99; obiclean_headcount=0; seq_rank=58; obiclean_samplecount=1; obiclean_status={'26a_F040644': 'i'}; obiclean_head=False; +ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII