annotate README.rst @ 0:6ea5a05a260a draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
author iuc
date Mon, 04 Mar 2019 10:15:02 -0500
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6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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1 The different methods from Scanpy have been grouped by themes:
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3 1. Filter in `filter.xml`
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4 - Filter cell outliers based on counts and numbers of genes expressed, using `pp.filter_cells`
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5 - Filter genes based on number of cells or counts, using `pp.filter_genes`
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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6 - Extract highly variable genes, using `pp.filter_genes_dispersion`
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7 - `tl.highly_variable_genes` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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8 - Subsample to a fraction of the number of observations, using `pp.subsample`
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9 - `queries.gene_coordinates` (need to be added)
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10 - `queries.mitochondrial_genes` (need to be added)
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11
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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12 2. Normalize in `normalize.xml`
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13 - Normalize total counts per cell, using `pp.normalize_per_cell`
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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14 - Normalization and filtering as of Zheng et al. (2017), using `pp.recipe_zheng17`
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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15 - Normalization and filtering as of Weinreb et al (2017), using `pp.recipe_weinreb17`
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16 - Normalization and filtering as of Seurat et al (2015), using `pp.recipe_seurat`
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17 - Logarithmize the data matrix, using `pp.log1p`
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18 - Scale data to unit variance and zero mean, using `pp.scale`
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19 - Square root the data matrix, using `pp.sqrt`
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20 - Downsample counts, using `pp.downsample_counts`
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22 3. Remove confounder in `remove_confounders.xml`
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23 - Regress out unwanted sources of variation, using `pp.regress_out`
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24 - `pp.mnn_correct` (need to be added)
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25 - `pp.mnn_correct` (need to be added)
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26 - `pp.magic` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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27 - `tl.sim` (need to be added)
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28 - `pp.calculate_qc_metrics` (need to be added)
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29 - Score a set of genes, using `tl.score_genes`
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30 - Score cell cycle genes, using `tl.score_genes_cell_cycle`
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31 - `tl.cyclone` (need to be added)
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32 - `tl.andbag` (need to be added)
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33
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34 4. Cluster and reduce dimension in `cluster_reduce_dimension.xml`
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35 - `tl.leiden` (need to be added)
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36 - Cluster cells into subgroups, using `tl.louvain`
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37 - Computes PCA (principal component analysis) coordinates, loadings and variance decomposition, using `pp.pca`
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38 - Computes PCA (principal component analysis) coordinates, loadings and variance decomposition, using `tl.pca`
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39 - Diffusion Maps, using `tl.diffmap`
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40 - t-distributed stochastic neighborhood embedding (tSNE), using `tl.tsne`
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41 - Embed the neighborhood graph using UMAP, using `tl.umap`
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42 - `tl.phate` (need to be added)
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43 - Compute a neighborhood graph of observations, using `pp.neighbors`
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44 - Rank genes for characterizing groups, using `tl.rank_genes_groups`
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45
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46 4. Inspect
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47 - `tl.paga_compare_paths` (need to be added)
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48 - `tl.paga_degrees` (need to be added)
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49 - `tl.paga_expression_entropies` (need to be added)
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50 - Generate cellular maps of differentiation manifolds with complex topologies, using `tl.paga`
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51 - Infer progression of cells through geodesic distance along the graph, using `tl.dpt`
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52
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53 5. Plot
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54 1. Generic
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55 - Scatter plot along observations or variables axes, using `pl.scatter`
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56 - Heatmap of the expression values of set of genes, using `pl.heatmap`
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57 - Makes a dot plot of the expression values, using `pl.dotplot`
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58 - Violin plot, using `pl.violin`
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59 - `pl.stacked_violin` (need to be added)
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60 - Heatmap of the mean expression values per cluster, using `pl.matrixplot`
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61 - Hierarchically-clustered heatmap, using `pl.clustermap`
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62 - `pl.ranking`
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63
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64 2. Preprocessing
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65 - Plot the fraction of counts assigned to each gene over all cells, using `pl.highest_expr_genes`
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66 - Plot dispersions versus means for genes, using `pl.filter_genes_dispersion`
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67 - `pl.highly_variable_genes` (need to be added)
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68 - `pl.calculate_qc_metrics` (need to be added)
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69
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70 3. PCA
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71 - Scatter plot in PCA coordinates, using `pl.pca`
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72 - Rank genes according to contributions to PCs, using `pl.pca_loadings`
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73 - Scatter plot in PCA coordinates, using `pl.pca_variance_ratio`
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74 - Plot PCA results, using `pl.pca_overview`
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75
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76 4. Embeddings
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77 - Scatter plot in tSNE basis, using `pl.tsne`
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78 - Scatter plot in UMAP basis, using `pl.umap`
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79 - Scatter plot in Diffusion Map basis, using `pl.diffmap`
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80 - `pl.draw_graph` (need to be added)
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81
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82 5. Branching trajectories and pseudotime, clustering
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83 - Plot groups and pseudotime, using `pl.dpt_groups_pseudotime`
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84 - Heatmap of pseudotime series, using `pl.dpt_timeseries`
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85 - Plot the abstracted graph through thresholding low-connectivity edges, using `pl.paga`
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86 - `pl.paga_compare` (need to be added)
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87 - `pl.paga_path` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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88
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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89 6. Marker genes:
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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90 - Plot ranking of genes using dotplot plot, using `pl.rank_gene_groups`
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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91 - `pl.rank_genes_groups_dotplot` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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92 - `pl.rank_genes_groups_heatmap` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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93 - `pl.rank_genes_groups_matrixplot` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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94 - `pl.rank_genes_groups_stacked_violin` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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95 - `pl.rank_genes_groups_violin` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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96
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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97 7. Misc
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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98 - `pl.phate` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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99 - `pl.matrix` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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100 - `pl.paga_adjacency` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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101 - `pl.timeseries` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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102 - `pl.timeseries_as_heatmap` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
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103 - `pl.timeseries_subplot` (need to be added)
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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104
6ea5a05a260a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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105