annotate seq2hla.xml @ 2:86d0ce0560a1 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
author iuc
date Tue, 07 Mar 2023 13:10:18 +0000
parents 5a7cd6c39085
children
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86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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1 <tool id="seq2hla" name="seq2HLA" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
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2 <description>HLA genotype and expression from RNA-seq</description>
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86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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3 <macros>
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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4 <token name="@TOOL_VERSION@">2.3</token>
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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5 <token name="@VERSION_SUFFIX@">0</token>
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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6 </macros>
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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7 <xrefs>
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8 <xref type="bio.tools">seq2hla</xref>
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9 </xrefs>
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10 <requirements>
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86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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11 <requirement type="package" version="@TOOL_VERSION@">seq2hla</requirement>
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12 </requirements>
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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13 <command detect_errors="exit_code"><![CDATA[
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86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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14 seq2HLA --runName='$run_name'
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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15 #if str( $fastq_input.fastq_input_selector ) == "paired":
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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16 -1 '${fastq_input.fastq_input1}'
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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17 -2 '${fastq_input.fastq_input2}'
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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18 #elif str( $fastq_input.fastq_input_selector ) == "paired_collection":
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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19 -1 '${fastq_input.fastq_input1.forward}'
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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20 -2 '${fastq_input.fastq_input1.reverse}'
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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21 #end if
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22 #if str($trim) != '':
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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23 --trim3=$trim
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24 #end if
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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25 -p \${GALAXY_SLOTS:-1} >> '$seq2hla_log'
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86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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26 && cp -p '${run_name}-ClassI-class.HLAgenotype2digits' '${c1_genotype2digits}'
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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27 && cp -p '${run_name}-ClassI-class.HLAgenotype4digits' '${c1_genotype4digits}'
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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28 && echo '#Locus_RPKM' | cat - '${run_name}-ClassI-class.expression' | sed 's/^\(.*\): \([0-9.]*\).*$/\1_\2/' | tr '_' '\t' > '${c1_expression}'
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29 && cp -p '${run_name}-ClassII.HLAgenotype4digits' '${c2_genotype4digits}'
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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30 && echo '#Locus_RPKM' | cat - '${run_name}-ClassII.expression' | sed 's/^\(.*\): \([0-9.]*\).*$/\1_\2/' | tr '_' '\t' > '${c2_expression}'
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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31 && cp -p '${run_name}.ambiguity' '${ambiguity}'
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32 ]]></command>
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33 <inputs>
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34 <param name="run_name" type="text" value="sample1" label="Name prefix for this analysis">
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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35 <validator type="regex" message="Use letters,digits,_.-">^[A-Za-z0-9_\-+.]+$</validator>
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36 </param>
2
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37 <conditional name="fastq_input">
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38 <param name="fastq_input_selector" type="select" label="Paired-end reads" help="Select between paired and paired-collection data">
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39 <option value="paired">Paired</option>
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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40 <option value="paired_collection">Paired Collection</option>
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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41 </param>
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42 <when value="paired">
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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43 <param name="fastq_input1" type="data" format="fastqsanger,fastqsanger.gz" label="Select first set of reads" help="Specify dataset with forward reads"/>
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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44 <param name="fastq_input2" type="data" format="fastqsanger,fastqsanger.gz" label="Select second set of reads" help="Specify dataset with reverse reads"/>
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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45 </when>
86d0ce0560a1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 3a6a342d9b5e0dd2774629079e315c8f33be7d29
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46 <when value="paired_collection">
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47 <param name="fastq_input1" format="fastqsanger,fastqsanger.gz" type="data_collection" collection_type="paired" label="Select a collection of paired-end reads"/>
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48 </when>
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49 </conditional>
0
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50 <param name="trim" type="integer" value="" min="0" optional="true" label="Trim bases from 3 prime"
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51 help="trim this many bases from the low-quality end of each read" />
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52 </inputs>
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53 <outputs>
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54 <data format="txt" name="seq2hla_log" label="${tool.name} on ${on_string}: ${run_name} logfile"/>
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55 <data format="tabular" name="c1_genotype2digits" label="${tool.name} on ${on_string}: ${run_name}-ClassI.HLAgenotype2digits"/>
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56 <data format="tabular" name="c1_genotype4digits" label="${tool.name} on ${on_string}: ${run_name}-ClassI.HLAgenotype4digits"/>
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57 <data format="tabular" name="c2_genotype4digits" label="${tool.name} on ${on_string}: ${run_name}-ClassII.HLAgenotype4digits"/>
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58 <data format="tabular" name="c1_expression" label="${tool.name} on ${on_string}: ${run_name}-ClassI.expression"/>
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59 <data format="tabular" name="c2_expression" label="${tool.name} on ${on_string}: ${run_name}-ClassII.expression"/>
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60 <data format="txt" name="ambiguity" label="${tool.name} on ${on_string}: ${run_name}.ambiguity"/>
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61 </outputs>
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62 <tests>
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63 <test>
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64 <param name="run_name" value="test"/>
2
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65 <conditional name="fastq_input">
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66 <param name="fastq_input_selector" value="paired"/>
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67 <param name="fastq_input1" ftype="fastqsanger" value="reads1.fastq"/>
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68 <param name="fastq_input2" ftype="fastqsanger" value="reads2.fastq"/>
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69 </conditional>
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70 <output name="c1_genotype4digits">
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71 <assert_contents>
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72 <has_text text="A*24:02" />
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73 </assert_contents>
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74 </output>
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75 </test>
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76 <test>
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77 <param name="run_name" value="test_collection"/>
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78 <conditional name="fastq_input">
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79 <param name="fastq_input_selector" value="paired_collection"/>
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80 <param name="fastq_input1">
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81 <collection type="paired">
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82 <element name="forward" ftype="fastqsanger" value="reads1.fastq" />
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83 <element name="reverse" ftype="fastqsanger" value="reads2.fastq" />
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84 </collection>
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85 </param>
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86 </conditional>
0
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87 <output name="c1_genotype4digits">
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88 <assert_contents>
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89 <has_text text="A*24:02" />
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90 </assert_contents>
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91 </output>
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92 </test>
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93 </tests>
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94 <help><![CDATA[
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95
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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96 **seq2HLA** *HLA typing from RNA-Seq sequence reads*
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97
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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98 Release: 2.2
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99
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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100 seq2HLA_ is an in-silico method, written in python and R, which takes standard RNA-Seq sequence reads in fastq format
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101 as input, uses a bowtie index comprising all HLA alleles and outputs the most likely HLA class I and class II genotypes (in 4 digit resolution),
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102 a p-value for each call, and the expression of each class
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103
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104 **Inputs**
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105
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106 Paired read fastq files with illumina style IDs.
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107
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108
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109 **Outputs**
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110
52bba1cd3823 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/seq2hla commit 96f219ed86ce674def72c73393d3c29c87cb56e5"
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111 1. <prefix>-ClassI.HLAgenotype2digits => 2 digit result of Class I
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112 2. <prefix>-ClassII.HLAgenotype2digits => 2 digit result of Class II
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113 3. <prefix>-ClassI.HLAgenotype4digits => 4 digit result of Class I
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114 4. <prefix>-ClassII.HLAgenotype4digits => 4 digit result of Class II
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115 5. <prefix>.ambiguity => reports typing ambuigities (more than one solution for an allele possible)
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116 6. <prefix>-ClassI.expression => expression of Class I alleles
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117 7. <prefix>-ClassII.expression => expression of Class II alleles
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118
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119
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120 ClassI.HLAgenotype4digits
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121
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122 ======= ======== =========== ======== ============
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123 #Locus Allele 1 Confidence Allele 2 Confidence
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124 ======= ======== =========== ======== ============
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125 A A*03:01 0.000510333 A*02:01' 0.0005975604
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126 B B*50:01 0.001271273 B*58:02 3.52561e-05
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127 C C*04:01 0.06362723 C*06:02 0.04725865
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128 ======= ======== =========== ======== ============
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129
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130
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131 ClassI.expression
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132
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133 ======= ======
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134 #Locus RPKM
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135 ======= ======
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136 A 89.59
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137 B 139.66
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138 C 184.42
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139 ======= ======
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140
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141
2
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142 .. _seq2HLA: https://github.com/TRON-Bioinformatics/seq2HLA
0
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143
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144 ]]></help>
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145 <citations>
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146 <citation type="doi">10.1186/gm403</citation>
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147 </citations>
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148 </tool>