annotate selection.xml @ 0:c369fea6eab5 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
author iuc
date Sun, 25 Aug 2024 13:35:59 +0000
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c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
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1 <tool id="spapros" name="Selection" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@profile@">
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2 <description>of marker genes with spapros</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements">
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7 </expand>
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8 <expand macro="version_command"/>
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9 <command detect_errors="exit_code"><![CDATA[
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10 @CMD@
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11 ]]></command>
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12 <configfiles>
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13 <configfile name="script_file"><![CDATA[
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14 @CMD_imports@
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15 @CMD_read_inputs@
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16
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17 random.seed($seed)
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18
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19 mpl.rcParams['figure.dpi'] = $general_figure_options.dpi
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20 plt.rcParams["font.size"] = $general_figure_options.fontsize
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21
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22 header_markerset='infer'
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23 #if $cond_markerset.select_markerset == 'True' and $cond_markerset.header_markerset == 'not_included':
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24 header_markerset=None
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25 #end if
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26
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27 selector = sp.se.ProbesetSelector(
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28 adata,
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29 celltype_key='$celltype_key',
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30 #if $genes_key != '':
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31 genes_key='$genes_key',
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32 #else:
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33 genes_key=None,
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34 #end if
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35 #if $cond_n.select_n == 'True':
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36 n=$cond_n.n,
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37 #else:
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38 n=None,
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39 #end if
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40 #if $preselected_genes != ''
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41 preselected_genes = '$preselected_genes'.split(','),
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42 #end if
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43 #if $prior_genes != ''
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44 prior_genes = '$prior_genes'.split(','),
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45 #end if
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46 #if $cond_n_pca_genes.select_n_pca_genes == 'True':
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47 n_pca_genes=$cond_n_pca_genes.n_pca_genes,
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48 #end if
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49 #if $min_mean_difference != 0.0:
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50 min_mean_difference=$min_mean_difference,
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51 #end if
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52 n_min_markers=$n_min_markers,
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53 #if $celltypes != 'all':
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54 celltypes='$celltypes'.split(','),
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55 #else
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56 celltypes='$celltypes',
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57 #end if
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58 #if $cond_markerset.select_markerset == 'True':
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59 marker_list = {key: [v for v in list(value.values()) if pd.notna(v)] for key, value in pd.read_csv('$cond_markerset.markerset', sep='\t', index_col=0, header=header_markerset).to_dict(orient='index').items()},
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60 #end if
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61 n_list_markers=$n_list_markers,
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62 marker_corr_th=$marker_corr_th,
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63 #if $pca_penalties != ''
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64 pca_penalties = '$pca_penalties'.split(','),
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65 #end if
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66 #if $DE_penalties != ''
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67 DE_penalties = '$DE_penalties'.split(','),
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68 #end if
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69 #if $m_penalties_adata_celltypes != ''
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70 m_penalties_adata_celltypes = '$m_penalties_adata_celltypes'.split(','),
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71 #end if
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72 #if $m_penalties_list_celltypes != ''
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73 m_penalties_list_celltypes = '$m_penalties_list_celltypes'.split(','),
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74 #end if
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75 #if $advanced_options.cond_DE_selection_hparams.select_DE_selection_hparams == 'True':
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76 DE_selection_hparams={"n": $advanced_options.cond_DE_selection_hparams.n_DE_selection_hparams, "per_group": $advanced_options.cond_DE_selection_hparams.per_group}
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77 #end if
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78 #if $advanced_options.cond_forest_hparams.select_forest_hparams == 'True':
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79 forest_hparams={"n_trees": $advanced_options.cond_forest_hparams.n_trees, "subsample": $advanced_options.cond_forest_hparams.subsample, "test_subsample": $advanced_options.cond_forest_hparams.test_subsample},
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80 #end if
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81 #if $advanced_options.cond_forest_DE_baseline_hparams.select_forest_DE_baseline_hparams == 'True':
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82 forest_DE_baseline_hparams={
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83 "n_DE": $advanced_options.cond_forest_DE_baseline_hparams.n_DE,
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84 "min_score": $advanced_options.cond_forest_DE_baseline_hparams.min_score,
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85 "n_stds": $advanced_options.cond_forest_DE_baseline_hparams.n_stds,
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86 "max_step": $advanced_options.cond_forest_DE_baseline_hparams.max_step,
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87 "min_outlier_dif": $advanced_options.cond_forest_DE_baseline_hparams.min_outlier_dif,
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88 "n_terminal_repeats": $advanced_options.cond_forest_DE_baseline_hparams.n_terminal_repeats,
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89 },
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90 #end if
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91 #if $advanced_options.cond_add_forest_genes_hparams.select_add_forest_genes_hparams == 'True':
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92 add_forest_genes_hparams={"n_max_per_it": $advanced_options.cond_add_forest_genes_hparams.n_max_per_it, "performance_th": $advanced_options.cond_add_forest_genes_hparams.performance_th, "importance_th": $advanced_options.cond_add_forest_genes_hparams.importance_th},
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93 #end if
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94 #if $advanced_options.cond_marker_selection_hparams.select_marker_selection_hparams == 'True':
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95 marker_selection_hparams={"penalty_threshold": $advanced_options.cond_marker_selection_hparams.penalty_threshold},
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96 #end if
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97 verbosity=0,
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98 seed=$seed,
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99 n_jobs=int(os.environ.get('GALAXY_SLOTS', '4'))
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100 )
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101
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102 selector.select_probeset()
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103
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104 sp.pl.masked_dotplot(
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105 adata,
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106 selector,
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107 ct_key='$figure_options_masked_dotplot.ct_key',
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108 imp_threshold=$figure_options_masked_dotplot.imp_threshold,
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109 #if $figure_options_masked_dotplot.celltypes != '':
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110 celltypes='$figure_options_masked_dotplot.celltypes',
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111 #end if
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112 #if $figure_options_masked_dotplot.n_genes != 0:
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113 n_genes=$figure_options_masked_dotplot.n_genes,
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114 #end if
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115 #if $figure_options_masked_dotplot.comb_markers_only:
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116 comb_markers_only=True,
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117 #end if
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118 #if $figure_options_masked_dotplot.markers_only:
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119 markers_only=True,
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120 #end if
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121 cmap='$figure_options_masked_dotplot.cmap',
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122 comb_marker_color='$figure_options_masked_dotplot.comb_marker_color',
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123 marker_color='$figure_options_masked_dotplot.marker_color',
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124 non_adata_celltypes_color='$figure_options_masked_dotplot.non_adata_celltypes_color',
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125 use_raw=$figure_options_masked_dotplot.use_raw,
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126 save='masked_dotplot.$format',
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127 )
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128
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129 selector.plot_gene_overlap(
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130 style='$figure_options_plot_gene_overlap.style',
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131 save='gene_overlap.$format',
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132 show=False
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133 )
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134
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135 probe_genes = selector.probeset.index[selector.probeset.selection]
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136 celltypes_DE_1vsall = list(selector.probeset[selector.probeset.selection]['celltypes_DE_1vsall'])
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137 celltypes_DE_specific = list(selector.probeset[selector.probeset.selection]['celltypes_DE_specific'])
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138 celltypes_DE = list(selector.probeset[selector.probeset.selection]['celltypes_DE'])
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139 celltypes_marker = list(selector.probeset[selector.probeset.selection]['celltypes_marker'])
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140
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141 marker_dict = dict()
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142
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143 for i,g in enumerate(probe_genes):
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144 recognized_celltypes = list(set(celltypes_DE_1vsall[i].split(',') + celltypes_DE_specific[i].split(',') + \
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145 celltypes_DE[i].split(',') + celltypes_marker[i].split(',')))
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146
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147 if (len(recognized_celltypes) > 1 and '' in recognized_celltypes):
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148 recognized_celltypes.remove('')
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149
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150 for c in recognized_celltypes:
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151 if c == '':
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152 c = 'Unkown'
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153 if c not in marker_dict:
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154 marker_dict[c] = [g]
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155 else:
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156 marker_dict[c] = marker_dict[c] + [g]
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157
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158 # Find the maximum length of lists
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159 max_len = max(len(lst) for lst in marker_dict.values())
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160 sorted_marker_dict_by_keys = {key: marker_dict[key] for key in sorted(marker_dict.keys())}
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161 # Fill smaller lists with empty values
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162 for key, value in sorted_marker_dict_by_keys.items():
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163 sorted_marker_dict_by_keys[key] = value + [''] * (max_len - len(value))
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164 df = pd.DataFrame(sorted_marker_dict_by_keys).T
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165 df.to_csv('marker.tsv', sep='\t', index=True)
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166
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167 ]]></configfile>
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168 </configfiles>
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169 <inputs>
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170 <expand macro="inputs_anndata"/>
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171 <expand macro="param_plot_format"/>
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172 <param name="celltype_key" type="text" value="celltype" optional="false" label="Key in adata.obs with celltype annotations"/>
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173 <param name="genes_key" type="text" optional="true" label="Key in adata.var for preselected genes" help="This is typically highly_variable_genes. Leave empty to not subset genes."/>
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174 <conditional name="cond_n">
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175 <param name="select_n" type="select" label="Do you want to set the number of finally selected genes?" help="Note that when `No` we automatically infer n as the minimal number of recommended genes.">
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176 <option value="False">No</option>
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177 <option value="True">Yes</option>
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178 </param>
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179 <when value="True">
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180 <param argument="n" type="integer" value="20" min="1" optional="false" label="Number of finally selected genes" help="Setting n might change the gene ranking since the final added list_markers are added based on the theoretically added genes without list_markers."/>
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181 </when>
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182 <when value="False"/>
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183 </conditional>
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184 <param name="preselected_genes" type="text" optional="true" label="Pre selected genes (comma separated)" help="These will also have the highest ranking in the final list."/>
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185 <param name="prior_genes" type="text" optional="true" label="Prioritized genes (comma separated)"/>
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186 <conditional name="cond_n_pca_genes">
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187 <param name="select_n_pca_genes" type="select" label="Do you want to set the number of preselected pca genes?" help="If not, then this step will be skipped.">
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188 <option value="True">Yes</option>
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189 <option value="False">No</option>
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190 </param>
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191 <when value="True">
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192 <param argument="n_pca_genes" type="integer" value="100" min="1" optional="false" label="Number of preselected pca genes"/>
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193 </when>
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194 <when value="False"/>
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195 </conditional>
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196 <param argument="min_mean_difference" type="float" value="0.0" optional="false" label="Minimal difference of mean expression between at least one celltype and the background" help="This minimal difference is applied as an additional binary penalty in pca_penalties, DE_penalties and m_penalties_adata_celltypes."/>
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197 <param argument="n_min_markers" type="integer" value="2" min="1" optional="false" label="The minimal number of identified and added markers"/>
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198 <param name="celltypes" type="text" value="all" optional="false" label="Cell types for which trees are trained" help="If not `all` then seperate the cell type with a comma (e.g., Glia,Neuron)"/>
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199 <conditional name="cond_markerset">
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200 <param name="select_markerset" type="select" label="Do you want to provide a set of marker genes?">
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201 <option value="False">No</option>
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202 <option value="True">Yes</option>
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203 </param>
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204 <when value="True">
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205 <param name="markerset" type="data" format="tabular" label="Markerset tabular file with rows=conditions (e.g., celltypes) and column=features (e.g., genes)"/>
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206 <param name="header_markerset" type="select" optional="false" label="Header in the list of markers?">
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207 <option value="included">Header included</option>
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208 <option value="not_included">Header not included</option>
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209 </param>
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210 </when>
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211 <when value="False"/>
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212 </conditional>
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213 <param argument="n_list_markers" type="integer" value="2" min="1" optional="false" label="Minimal number of markers per celltype that are at least selected" help="Selected means either selecting genes from the marker list or having correlated genes in the already selected panel."/>
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214 <param argument="marker_corr_th" type="float" value="0.5" optional="false" label="Minimal correlation to consider a gene as captured"/>
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215 <param name="pca_penalties" type="text" optional="true" label="List of keys for columns in adata.var containing penalty factors that are multiplied with the scores for PCA based gene selection" help="(comma separted)"/>
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216 <param name="DE_penalties" type="text" optional="true" label="List of keys for columns in adata.var containing penalty factors that are multiplied with the scores for DE based gene selection (comma separted)" help="(comma separted)"/>
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217 <param name="m_penalties_adata_celltypes" type="text" optional="true" label="List of keys for columns in adata.var containing penalty factors to filter out marker genes if a gene's penalty &lt; threshold for celltypes in adata" help="(comma separted)"/>
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218 <param name="m_penalties_list_celltypes" type="text" optional="true" label="List of keys for columns in adata.var containing penalty factors to filter out marker genes if a gene's penalty &lt; threshold for celltypes **not** in adata" help="(comma separted)"/>
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219 <param argument="seed" type="integer" value="123" min="0" optional="false" label="Random number seed"/>
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220
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221 <section name="advanced_options" title="Advanced Options" expanded="false">
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222 <conditional name="cond_DE_selection_hparams">
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223 <param name="select_DE_selection_hparams" type="select" label="Do you want to tune hyperparameters for the DE based gene selection?">
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224 <option value="False">No</option>
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225 <option value="True">Yes</option>
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226 </param>
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227 <when value="True">
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228 <param argument="n_DE_selection_hparams" type="integer" value="3" optional="false" label="n"/>
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229 <param name="per_group" type="select" label="per_group">
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230 <option value="False">No</option>
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231 <option value="True">Yes</option>
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232 </param>
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233 </when>
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234 <when value="False"/>
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235 </conditional>
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236 <conditional name="cond_forest_hparams">
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237 <param name="select_forest_hparams" type="select" label="Do you want to tune hyperparameters for the forest based gene selection?">
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238 <option value="False">No</option>
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239 <option value="True">Yes</option>
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240 </param>
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241 <when value="True">
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242 <param argument="n_trees" type="integer" value="50" optional="false" label="n_trees"/>
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243 <param argument="subsample" type="integer" value="1000" optional="false" label="subsample"/>
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244 <param argument="test_subsample" type="integer" value="3000" optional="false" label="test_subsample"/>
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245 </when>
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246 <when value="False"/>
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247 </conditional>
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248 <conditional name="cond_forest_DE_baseline_hparams">
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249 <param name="select_forest_DE_baseline_hparams" type="select" label="Do you want to tune hyperparameters for the DE based gene selection?">
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250 <option value="False">No</option>
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251 <option value="True">Yes</option>
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252 </param>
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253 <when value="True">
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254 <param argument="n_DE" type="integer" value="1" optional="false" label="n_DE"/>
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255 <param argument="min_score" type="float" value="0.9" optional="false" label="min_score"/>
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256 <param argument="n_stds" type="float" value="1.0" optional="false" label="n_stds"/>
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257 <param argument="max_step" type="integer" value="3" optional="false" label="max_step"/>
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258 <param argument="min_outlier_dif" type="float" value="0.02" optional="false" label="min_outlier_dif"/>
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259 <param argument="n_terminal_repeats" type="integer" value="3" optional="false" label="n_terminal_repeats"/>
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260 </when>
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261 <when value="False"/>
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262 </conditional>
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263 <conditional name="cond_add_forest_genes_hparams">
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264 <param name="select_add_forest_genes_hparams" type="select" label="Do you want to tune hyperparameters for adding marker genes to decision trees?">
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265 <option value="False">No</option>
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266 <option value="True">Yes</option>
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267 </param>
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268 <when value="True">
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269 <param argument="n_max_per_it" type="integer" value="5" optional="false" label="n_max_per_it"/>
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270 <param argument="performance_th" type="float" value="0.02" optional="false" label="performance_th"/>
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271 <param argument="importance_th" type="integer" value="0" optional="false" label="importance_th"/>
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272 </when>
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273 <when value="False"/>
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274 </conditional>
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275 <conditional name="cond_marker_selection_hparams">
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276 <param name="select_marker_selection_hparams" type="select" label="Do you want to tune marker selection hyperparameters?">
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277 <option value="False">No</option>
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278 <option value="True">Yes</option>
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279 </param>
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280 <when value="True">
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281 <param argument="penalty_threshold" type="integer" value="1" optional="false" label="penalty_threshold"/>
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282 </when>
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283 <when value="False"/>
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284 </conditional>
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285 </section>
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286
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287 <section name="general_figure_options" title="General Figure Output Options" expanded="false">
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288 <param argument="dpi" type="integer" value="300" min="1" label="Dpi of figures"/>
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289 <param argument="fontsize" type="integer" value="100" min="1" label="Font size of figures"/>
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290 </section>
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291
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292 <section name="figure_options_masked_dotplot" title="Figure Output Options for masked_dotplot" expanded="false">
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293 <param name="ct_key" type="text" value="celltype" optional="false" label="Key in adata.var for preselected genes" help="Column of adata.obs with cell type annotation"/>
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294 <param argument="imp_threshold" type="float" value="0.05" min="0.0" optional="false" label="Annotate genes as Spapros marker only for those genes with importance > imp_threshold"/>
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295 <param name="celltypes" type="text" optional="true" label="Subset of celltypes (rows of dotplot)"/>
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296 <param argument="n_genes" type="integer" value="0" min="0" label="Plot top n_genes genes." help="If 0 then all."/>
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297 <param name="comb_markers_only" type="boolean" value="false" label="Do you want to plot only genes that are Spapros markers for the plotted cell types?"/>
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298 <param name="markers_only" type="boolean" value="false" label="Do you want to plot only genes that are markers for the plotted cell types?"/>
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299 <param name="cmap" type="text" value="Reds" optional="false" label="Colormap of mean expressions"/>
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300 <param name="comb_marker_color" type="text" value="darkblue" optional="false" label="Color for Spapros markers"/>
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301 <param name="marker_color" type="text" value="blue" optional="false" label="Color for marker genes"/>
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302 <param name="non_adata_celltypes_color" type="text" value="grey" optional="false" label="Color for celltypes that don't occur in the data set."/>
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303 <param name="use_raw" type="select" label="Do you want to use adata.raw for plotting?">
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304 <option value="False">No</option>
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305 <option value="True">Yes</option>
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306 </param>
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307 </section>
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308
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309 <section name="figure_options_plot_gene_overlap" title="Figure Output Options for plot_gene_overlap" expanded="false">
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310 <param name="style" type="select" label="Plot type">
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311 <option value="upset">Upset plot</option>
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312 <option value="venn">Venn diagram</option>
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313 </param>
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314 </section>
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315
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316 <expand macro="inputs_common_advanced"/>
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317 </inputs>
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318 <outputs>
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319 <data name="out_masked_dotplot_png" format="png" from_work_dir="*masked_dotplot.png" label="PNG masked_dotplot from ${tool.name} on ${on_string}">
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320 <filter>format == 'png'</filter>
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321 </data>
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322 <data name="out_masked_dotplot_pdf" format="pdf" from_work_dir="*masked_dotplot.pdf" label="PDF masked_dotplot from ${tool.name} on ${on_string}">
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323 <filter>format == 'pdf'</filter>
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324 </data>
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325 <data name="out_masked_dotplot_svg" format="svg" from_work_dir="*masked_dotplot.svg" label="SVG masked_dotplot from ${tool.name} on ${on_string}">
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326 <filter>format == 'svg'</filter>
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327 </data>
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328 <data name="out_gene_overlap_png" format="png" from_work_dir="*gene_overlap.png" label="PNG gene_overlap from ${tool.name} on ${on_string}">
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329 <filter>format == 'png'</filter>
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330 </data>
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331 <data name="out_gene_overlap_pdf" format="pdf" from_work_dir="*gene_overlap.pdf" label="PDF gene_overlap from ${tool.name} on ${on_string}">
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332 <filter>format == 'pdf'</filter>
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333 </data>
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334 <data name="out_gene_overlap_svg" format="svg" from_work_dir="*gene_overlap.svg" label="SVG gene_overlap from ${tool.name} on ${on_string}">
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335 <filter>format == 'svg'</filter>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
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diff changeset
336 </data>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
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diff changeset
337 <data name="marker_out" format="tabular" from_work_dir="marker.tsv" label="${tool.name} on ${on_string}: Markers"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
338 <expand macro="hidden_outputs"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
339 </outputs>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
340 <tests>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
341 <test expect_num_outputs="4">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
342 <param name="adata" value="tl.rank_genes_groups.newton-cg.pbmc68k_reduced_240cells.h5ad"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
343 <param name="format" value="png"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
344 <param name="genes_key" value="highly_variable"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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345 <param name="show_log" value="true" />
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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346 <section name="general_figure_options">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
347 <param name="dpi" value="100"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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348 </section>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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349 <output name="hidden_output">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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350 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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351 <has_text_matching expression="celltype_key='celltype',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
352 <has_text_matching expression="genes_key='highly_variable',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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353 <has_text_matching expression="n_pca_genes=100,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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354 <has_text_matching expression="seed=123,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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355 <has_text_matching expression="cmap='Reds',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
356 <has_text_matching expression="save='masked_dotplot.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
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357 <has_text_matching expression="style='upset',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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358 <has_text_matching expression="save='gene_overlap.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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359 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
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360 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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361 <output name="out_masked_dotplot_png">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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362 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
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363 <has_image_width width="4055" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
364 <has_image_height height="1108" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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365 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
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diff changeset
366 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
367 <output name="out_gene_overlap_png">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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368 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
369 <has_image_width width="1189" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
370 <has_image_height height="600" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
371 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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372 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
373 <output name="marker_out" file="marker_out_test1.tsv" ftype="tabular"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
374 </test>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
375 <test expect_num_outputs="4">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
376 <param name="adata" value="tl.rank_genes_groups.newton-cg.pbmc68k_reduced_240cells.h5ad"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
377 <param name="format" value="png"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
378 <param name="genes_key" value="highly_variable"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
379 <param name="select_n" value="True"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
380 <param name="n" value="10"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
381 <section name="general_figure_options">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
382 <param name="dpi" value="100"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
383 </section>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
384 <param name="show_log" value="true" />
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
385 <output name="hidden_output">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
386 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
387 <has_text_matching expression="celltype_key='celltype',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
388 <has_text_matching expression="genes_key='highly_variable',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
389 <has_text_matching expression="n_pca_genes=100,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
390 <has_text_matching expression="n=10,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
391 <has_text_matching expression="seed=123,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
392 <has_text_matching expression="cmap='Reds',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
393 <has_text_matching expression="save='masked_dotplot.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
394 <has_text_matching expression="style='upset',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
395 <has_text_matching expression="save='gene_overlap.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
396 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
397 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
398 <output name="out_masked_dotplot_png">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
399 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
400 <has_image_width width="2914" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
401 <has_image_height height="882" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
402 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
403 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
404 <output name="out_gene_overlap_png">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
405 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
406 <has_image_width width="1032" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
407 <has_image_height height="600" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
408 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
409 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
410 <output name="marker_out" file="marker_out_test2.tsv" ftype="tabular"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
411 </test>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
412 <test expect_num_outputs="4">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
413 <param name="adata" value="tl.rank_genes_groups.newton-cg.pbmc68k_reduced_240cells.h5ad"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
414 <param name="format" value="png"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
415 <param name="genes_key" value="highly_variable"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
416 <param name="celltypes" value="CD34+,CD56+ NK"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
417 <section name="general_figure_options">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
418 <param name="dpi" value="100"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
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diff changeset
419 </section>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
420 <param name="show_log" value="true" />
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
421 <output name="hidden_output">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
422 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
423 <has_text_matching expression="celltype_key='celltype',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
424 <has_text_matching expression="genes_key='highly_variable',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
425 <has_text_matching expression="n_pca_genes=100,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
426 <has_text_matching expression="seed=123,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
427 <has_text_matching expression="cmap='Reds',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
428 <has_text_matching expression="save='masked_dotplot.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
429 <has_text_matching expression="style='upset',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
430 <has_text_matching expression="save='gene_overlap.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
431 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
432 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
433 <output name="out_masked_dotplot_png">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
434 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
435 <has_image_width width="2776" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
436 <has_image_height height="882" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
437 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
438 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
439 <output name="out_gene_overlap_png">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
440 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
441 <has_image_width width="929" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
442 <has_image_height height="565" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
443 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
444 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
445 <output name="marker_out" file="marker_out_test3.tsv" ftype="tabular"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
446 </test>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
447 <test expect_num_outputs="4">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
448 <param name="adata" value="tl.rank_genes_groups.newton-cg.pbmc68k_reduced_240cells.h5ad"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
449 <param name="format" value="png"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
450 <param name="genes_key" value="highly_variable"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
451 <param name="select_markerset" value="True"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
452 <param name="markerset" value="marker.tsv"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
453 <param name="header_markerset" value="not_included"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
454 <section name="general_figure_options">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
455 <param name="dpi" value="100"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
456 </section>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
457 <param name="show_log" value="true"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
458 <output name="hidden_output">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
459 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
460 <has_text_matching expression="celltype_key='celltype',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
461 <has_text_matching expression="genes_key='highly_variable',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
462 <has_text_matching expression="n_pca_genes=100,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
463 <has_text_matching expression="seed=123,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
464 <has_text_matching expression="cmap='Reds',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
465 <has_text_matching expression="save='masked_dotplot.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
466 <has_text_matching expression="style='upset',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
467 <has_text_matching expression="save='gene_overlap.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
468 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
469 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
470 <output name="out_masked_dotplot_png">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
471 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
472 <has_image_width width="4055" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
473 <has_image_height height="1108" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
474 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
475 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
476 <output name="out_gene_overlap_png">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
477 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
478 <has_image_width width="1154" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
479 <has_image_height height="600" delta="2"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
480 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
481 </output>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
482 <output name="marker_out" file="marker_out_test4.tsv" ftype="tabular"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
483 </test>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
484 <test expect_num_outputs="4">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
485 <param name="adata" value="tl.rank_genes_groups.newton-cg.pbmc68k_reduced_240cells.h5ad"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
486 <param name="format" value="png"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
487 <param name="genes_key" value="highly_variable"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
488 <section name="advanced_options">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
489 <param name="select_DE_selection_hparams" value="True"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
490 <param name="select_forest_hparams" value="True"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
491 <param name="select_forest_DE_baseline_hparams" value="True"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
492 <param name="select_add_forest_genes_hparams" value="True"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
493 <param name="select_marker_selection_hparams" value="True"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
494 </section>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
495 <section name="general_figure_options">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
496 <param name="dpi" value="100"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
497 </section>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
498 <param name="show_log" value="true"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
499 <output name="hidden_output">
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
500 <assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
501 <has_text_matching expression="celltype_key='celltype',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
502 <has_text_matching expression="genes_key='highly_variable',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
503 <has_text_matching expression="n_pca_genes=100,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
504 <has_text_matching expression="seed=123,"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
505 <has_text_matching expression="cmap='Reds',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
506 <has_text_matching expression="save='masked_dotplot.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
507 <has_text_matching expression="style='upset',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
508 <has_text_matching expression="save='gene_overlap.png',"/>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
diff changeset
509 </assert_contents>
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
iuc
parents:
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510 </output>
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511 <output name="out_masked_dotplot_png">
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512 <assert_contents>
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513 <has_image_width width="4055" delta="2"/>
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514 <has_image_height height="1108" delta="2"/>
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515 </assert_contents>
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516 </output>
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517 <output name="out_gene_overlap_png">
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518 <assert_contents>
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519 <has_image_width width="1189" delta="2"/>
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520 <has_image_height height="600" delta="2"/>
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521 </assert_contents>
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522 </output>
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523 <output name="marker_out" file="marker_out_test5.tsv" ftype="tabular"/>
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524 </test>
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525 </tests>
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526 <help><![CDATA[
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
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527 Probe set selection for single-cell sequencing data using spapros.
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528 ============================================================================================================
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529
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
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530 Spapros is a python package that provides a pipeline for probe set selection and evaluation for targeted spatial transcriptomics data.
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531
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
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532 Key Features:
c369fea6eab5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spapros/ commit a773446d7e104d987bfd01ccdcc1d80066c504f0
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533 Select probe sets for spatial transcriptomics which identify cell types of interest, capture general transcriptomic variation, and incorporate prior knowledge
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534
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535 Evaluate probe sets with an extensive pipeline
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536
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537 Further documentation can be found here: https://spapros.readthedocs.io/en/latest/index.html.
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538
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539 ]]></help>
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540 <expand macro="citations"/>
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541 </tool>