Mercurial > repos > iuc > stacks2_refmap

annotate stacks_refmap.xml @ 6:33f0793edf93 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 2f4c9bfc48d63075ae18a1687e8d01ffea509084
author iuc
date Wed, 11 May 2022 06:43:52 +0000
parents 887e0991eb24
children
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887e0991eb24 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 16654f3b3faaba994cea5fda8d4907ea4f059605"
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1 <tool id="stacks2_refmap" name="Stacks2: reference map" profile="@PROFILE@" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
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2 <description>the Stacks pipeline with a reference genome (ref_map.pl)</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements"/>
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7 <expand macro="version_cmd"/>
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8 <command detect_errors="aggressive"><![CDATA[
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9 @FASTQ_INPUT_FUNCTIONS@
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10 mkdir bam_inputs stacks_outputs &&
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12 #if $output_log
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13 ln -s '$output_log' stacks_outputs/ref_map.log &&
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14 #end if
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15
0
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16 @BAM_INPUT@
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18 ref_map.pl
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19 --samples bam_inputs
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20 #if str($popmap) != 'None':
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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21 --popmap '$popmap'
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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22 #end if
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23 $paired_select
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24 -o stacks_outputs
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25 -T \${GALAXY_SLOTS:-1}
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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26 --var-alpha $model_options.var_alpha
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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27 --gt-alpha $model_options.gt_alpha
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28
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29 @EXTRACT_VCF@
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30 ]]></command>
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31
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32 <inputs>
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33 <expand macro="bam_input_macro"/>
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34 <param argument="--popmap" type="data" optional="true" format="tabular,txt" label="Population map"/>
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45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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35 <param name="paired_select" type="select" label="Paired end options" help="select single/paired for single end data or to select advanced paired end options, --unpaired: treat reverse reads as if they were forward reads; --ignore-pe-reads: ignore paired-end reads even if present in the input">
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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36 <option value="" selected="true">single/paired</option>
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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37 <option value="--unpaired" selected="true">ignore read pairing (--unpaired)</option>
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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38 <option value="--ignore-pe-reads" selected="true">ignore paired-end reads (--ignore-pe-reads)</option>
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39 </param>
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40 <section name="model_options" title="Variant calling options (for gstacks)" expanded="true">
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41 <expand macro="variant_calling_options_vg" varalpha_default="0.01"/>
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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42 </section>
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43 <expand macro="in_log"/>
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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44 </inputs>
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45 <outputs>
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46 <expand macro="out_log"/> <!-- pipeline also writes other logs? .. could be a collection -->
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47 <expand macro="gstacks_outputs_macro" tooladd="(gstacks)"/>
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48 <expand macro="populations_output_light" tooladd="(populations)"/>
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49 </outputs>
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50
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51 <tests>
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52 <!-- bams as list -->
2
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53 <test expect_num_outputs="7">
0
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54 <param name="input_bam" ftype="bam" value="refmap/PopA_01.bam,refmap/PopA_02.bam"/>
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55 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv"/>
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56 <param name="add_log" value="yes"/>
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57 <output ftype="txt" name="output_log"><assert_contents><has_text text="ref_map.pl is done."/></assert_contents></output>
0
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58 <output_collection name="gstacks_out" type="list" count="2">
2
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59 <element name="catalog.calls.vcf" file="refmap/catalog.calls.vcf" ftype="vcf" lines_diff="4"/>
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60 <element name="catalog.fa.gz" file="refmap/catalog.fa.gz" ftype="fasta.gz" compare="diff"/>
0
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61 </output_collection>
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62 <output ftype="tabular" name="out_haplotypes" value="refmap/populations.haplotypes.tsv"/>
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63 <output ftype="tabular" name="out_hapstats" value="refmap/populations.hapstats.tsv"/>
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64 <output ftype="txt" name="out_populations_log_distribs" value="refmap/populations.log.distribs"/>
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65 <output ftype="tabular" name="out_sumstats_sum" value="refmap/populations.sumstats_summary.tsv"/>
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66 <output ftype="tabular" name="out_sumstats" value="refmap/populations.sumstats.tsv"/>
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67 </test>
2
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68 <test expect_num_outputs="7">
0
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69 <param name="input_bam" ftype="bam" value="refmap/PopA_01.bam,refmap/PopA_02.bam"/>
2
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70 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv"/>
0
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71 <param name="paired_select" value="--unpaired"/>
2
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72 <param name="model_options|var_alpha" value="0.1"/>
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73 <param name="model_options|gt_alpha" value="0.1"/>
0
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74 <assert_command>
2
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75 <has_text text="--unpaired"/>
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76 <has_text text="--var-alpha 0.1"/>
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77 <has_text text="--gt-alpha 0.1"/>
0
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78 </assert_command>
2
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79 <param name="add_log" value="yes"/>
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80 <output ftype="txt" name="output_log"><assert_contents><has_text text="ref_map.pl is done."/></assert_contents></output>
0
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81 <output_collection name="gstacks_out" type="list" count="2"/>
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82 <output ftype="tabular" name="out_haplotypes"><assert_contents><has_text text="#"/></assert_contents></output>
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83 <output ftype="tabular" name="out_hapstats"><assert_contents><has_text text="#"/></assert_contents></output>
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84 <output ftype="txt" name="out_populations_log_distribs"><assert_contents><has_text text="#"/></assert_contents></output>
45385ba40a75 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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85 <output ftype="tabular" name="out_sumstats_sum"><assert_contents><has_text text="#"/></assert_contents></output>
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86 <output ftype="tabular" name="out_sumstats"><assert_contents><has_text text="#"/></assert_contents></output>
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87
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88 </test>
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89 </tests>
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90
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91 <help>
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92 <![CDATA[
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93 .. class:: infomark
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94
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95 **What it does**
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96
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97 This program expects data that have been aligned to a reference genome, and can accept data directly from Bowtie, or from any aligner that can produce SAM format. To avoid datasets names problems, we recommand the use of the *Map with BWA for STACKS tool*. This program will execute each of the Stacks components: first, running pstacks on each of the samples specified, building loci (based on the reference alignment) and calling SNPs in each. Second, cstacks will be run to create a catalog of all loci specified as 'parents' or 'samples' on the command line, again using alignment to match loci in the catalog. Finally, sstacks will be executed to match each sample against the catalog. The ref_map.pl program will also load the results of each stage of the analysis: individual loci, the catalog, and matches against the catalog into the database (although this can be disabled). After matching the program will build a database index to speed up access (index_radtags.pl) and enable web-based filtering.
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98
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99 --------
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100
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101 **Input files**
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102
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103 - SAM, BAM
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104
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105 - Population map::
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106
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107 indv_01 1
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108 indv_02 1
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109 indv_03 1
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110 indv_04 2
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111 indv_05 2
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112 indv_06 2
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113
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114 **Output files**
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115
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116 - XXX.tags.tsv file:
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117
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118 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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119
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120 Notes: For the tags file, each stack will start in the file with a consensus sequence for the entire stack followed by the flags for that stack. Then, each individual read that was merged into that stack will follow. The next stack will start with another consensus sequence.
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121
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122
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123 - XXX.snps.tsv file:
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124
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125 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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126
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127 Notes: If a stack has two SNPs called within it, then there will be two lines in this file listing each one.
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128
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129
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130 - XXX.alleles.tsv file:
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131
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132 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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133
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134
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135 - XXX.matches.tsv file:
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136
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137 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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138
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139 Notes: Each line in this file records a match between a catalog locus and a locus in an individual, for a particular haplotype. The Batch ID plus the Catalog ID together represent a unique locus in the entire population, while the Sample ID and the Stack ID together represent a unique locus in an individual sample.
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140
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141
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142 - other files:
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143
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144 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_
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145
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146 @STACKS_INFOS@
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147 ]]>
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148 </help>
2
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149 <expand macro="citation"/>
0
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150 </tool>