annotate stringtie.xml @ 21:914cfcba0ec8 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
author iuc
date Mon, 31 Oct 2022 20:08:02 +0000
parents cef79931dda5
children ae618321f34a
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
19
333a6e13b622 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a586aaa6ef561527cda9e922a2260aa591f43938"
iuc
parents: 18
diff changeset
1 <tool id="stringtie" name="StringTie" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
2
520e0988ec1c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b03fd81ed11744f119608d4884c24fa182eb605a
iuc
parents: 1
diff changeset
2 <description>transcript assembly and quantification</description>
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
3 <xrefs>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
4 <xref type="bio.tools">stringtie</xref>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
5 </xrefs>
6
d0bb240d7525 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit acdc3dfe5fd6317ae57899aa0e83130dff7460e7
iuc
parents: 5
diff changeset
6 <macros>
d0bb240d7525 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit acdc3dfe5fd6317ae57899aa0e83130dff7460e7
iuc
parents: 5
diff changeset
7 <import>macros.xml</import>
d0bb240d7525 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit acdc3dfe5fd6317ae57899aa0e83130dff7460e7
iuc
parents: 5
diff changeset
8 </macros>
d0bb240d7525 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit acdc3dfe5fd6317ae57899aa0e83130dff7460e7
iuc
parents: 5
diff changeset
9 <expand macro="requirements" />
d0bb240d7525 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit acdc3dfe5fd6317ae57899aa0e83130dff7460e7
iuc
parents: 5
diff changeset
10 <expand macro="stdio" />
d0bb240d7525 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit acdc3dfe5fd6317ae57899aa0e83130dff7460e7
iuc
parents: 5
diff changeset
11 <expand macro="version_command" />
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
12 <command><![CDATA[
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
13 #import re
21
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
14 #set $compressed = ("bam","cram")
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
15 mkdir -p ./special_de_output/sample1/ &&
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
16
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
17 ## Get Guide GTF/GFF if selected
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
18
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
19 #if str($guide.use_guide) == 'yes':
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
20 #if $guide.guide_source.guide_gff_select == "history":
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
21 ln -s '$guide.guide_source.ref_hist' guide.gff &&
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
22 #elif $guide.guide_source.guide_gff_select == "cached":
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
23 ln -s '$guide.guide_source.ref_builtin.fields.path' guide.gff &&
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
24 #end if
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
25 #end if
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
26
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
27 #if $input_options.input_mode in ['short_reads','long_reads']:
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
28 #if $input_options.input_bam.metadata.ftype == 'sam':
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
29 samtools sort -@ \${GALAXY_SLOTS:-1} '$input_options.input_bam' -T "\${TMPDIR:-.}" | stringtie
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
30 #else
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
31 stringtie '$input_options.input_bam'
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
32 #end if
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
33 #if $input_options.input_mode == 'long_reads'
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
34 -L
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
35 -E $input_options.error_splice
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
36 #end if
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
37 #else
21
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
38 #if $input_options.input_bam_short.file_ext in $compressed and $input_options.input_bam_long.file_ext in $compressed:
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
39 stringtie '$input_options.input_bam_short' '$input_options.input_bam_long'
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
40 #else if $input_options.input_bam_short.metadata.ftype == 'sam' and $input_options.input_bam_long.metadata.ftype in $compressed:
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
41 samtools sort -@ \${GALAXY_SLOTS:-1} '$input_options.input_bam_short' -T "\${TMPDIR:-.}" -o short_sorted.sam
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
42 && stringtie short_sorted.sam '$input_options.input_bam_long'
21
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
43 #else if $input_options.input_bam_short.metadata.ftype in $compressed and $input_options.input_bam_long.metadata.ftype == 'sam':
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
44 samtools sort -@ \${GALAXY_SLOTS:-1} '$input_options.input_bam_long' -T "\${TMPDIR:-.}" -o long_sorted.sam
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
45 && stringtie'$input_options.input_bam_short' long_sorted.sam
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
46 #else
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
47 samtools sort -@ \${GALAXY_SLOTS:-1} '$input_options.input_bam_short' -T "\${TMPDIR:-.}" -o short_sorted.sam
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
48 && samtools sort -@ \${GALAXY_SLOTS:-1} '$input_options.input_bam_long' -T "\${TMPDIR:-.}" -o long_sorted.sam
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
49 && stringtie short_sorted.sam long_sorted.sam
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
50 #end if
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
51 -E $input_options.error_splice
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
52 #end if
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
53
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
54 -o '$output_gtf'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
55 -p "\${GALAXY_SLOTS:-1}"
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
56
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
57 $rna_strandness
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
58
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
59 #if str($guide.use_guide) == 'yes':
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
60 -G guide.gff
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
61 #if $guide.coverage_file:
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
62 -C '$coverage'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
63 #end if
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
64 $guide.input_estimation
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
65 #if $guide.special_outputs != 'no':
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
66 -b ./special_de_output/sample1/
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
67 #end if
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
68 #end if
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
69
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
70 #if $adv.name_prefix:
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
71 -l '$adv.name_prefix'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
72 #end if
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
73 -f '$adv.fraction'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
74 -m '$adv.min_tlen'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
75 -a '$adv.min_anchor_len'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
76 -j '$adv.min_anchor_cov'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
77 -c '$adv.min_bundle_cov'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
78 -g '$adv.bdist'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
79 -M '$adv.bundle_fraction'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
80 $adv.disable_trimming
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
81 $adv.multi_mapping
19
333a6e13b622 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a586aaa6ef561527cda9e922a2260aa591f43938"
iuc
parents: 18
diff changeset
82 #if $adv.point_features
333a6e13b622 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a586aaa6ef561527cda9e922a2260aa591f43938"
iuc
parents: 18
diff changeset
83 --ptf $adv.point_features
333a6e13b622 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a586aaa6ef561527cda9e922a2260aa591f43938"
iuc
parents: 18
diff changeset
84 #end if
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
85 #if $adv.abundance_estimation:
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
86 -A '$gene_abundance_estimation'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
87 #end if
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
88 #if str($adv.omit_sequences).strip() != "":
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
89 -x '$adv.omit_sequences'
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
90 #end if
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
91
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
92 #if str($guide.use_guide) == 'yes':
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
93 #if $guide.special_outputs.special_outputs_select == 'deseq2':
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
94 #set escaped_element_identifier = re.sub('[^\w\-]', '_', str($input_bam.element_identifier))
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
95 &&
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
96 ln -s '$output_gtf' ./special_de_output/sample1/output.gtf
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
97 &&
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
98 TAB=\$(printf '\t')
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
99 &&
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
100 CR=\$(printf '\r')
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
101 &&
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
102 prepDE.py
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
103 -i ./special_de_output/
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
104 -g gene_counts.csv
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
105 -t transcript_counts.csv
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
106 -l $guide.special_outputs.read_length
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
107 #if $guide.special_outputs.string:
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
108 -s '$guide.special_outputs.string'
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
109 #end if
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
110 #if $guide.special_outputs.clustering:
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
111 -c
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
112 #if $guide.special_outputs.key:
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
113 -k '$guide.special_outputs.key'
6
d0bb240d7525 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit acdc3dfe5fd6317ae57899aa0e83130dff7460e7
iuc
parents: 5
diff changeset
114 #end if
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
115 --legend '$legend'
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
116 > /dev/null
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
117 &&
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
118 sed -i.bak -e "s/,/\${TAB}/g" -e "s/\${CR}//g" '$legend'
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
119 #else
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
120 > /dev/null
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
121 #end if
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
122
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
123 ## Replace commas with tabs
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
124 &&
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
125 sed -i.bak -e "s/,/\${TAB}/g" -e "s/\${CR}//g" gene_counts.csv transcript_counts.csv
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
126 ## Output header
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
127 &&
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
128 head -n 1 gene_counts.csv | sed -e 's/sample1/$escaped_element_identifier/' > '$gene_counts'
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
129 &&
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
130 head -n 1 transcript_counts.csv | sed -e 's/sample1/$escaped_element_identifier/' > '$transcript_counts'
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
131 ## Sort count files on the first column
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
132 &&
14
eafd5dc95228 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 57441e46de7ca04f25df6e43152dc210d4b32712
iuc
parents: 13
diff changeset
133 tail -n +2 gene_counts.csv | sort -t"\${TAB}" -k1,1 >> '$gene_counts'
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
134 &&
14
eafd5dc95228 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 57441e46de7ca04f25df6e43152dc210d4b32712
iuc
parents: 13
diff changeset
135 tail -n +2 transcript_counts.csv | sort -t"\${TAB}" -k1,1 >> '$transcript_counts'
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
136 #end if
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
137 #end if
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
138 ]]></command>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
139 <inputs>
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
140 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
141 <param name="input_mode" type="select" label="Input options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
142 <option value="short_reads">Short reads</option>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
143 <option value="long_reads">Long reads</option>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
144 <option value="mixed_reads">Mixed mode: short and long reads</option>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
145 </param>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
146 <when value="short_reads">
21
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
147 <param name="input_bam" type="data" format="sam,bam,cram" label="Input short mapped reads" help="Input BAM/SAM/CRAM file containing the short reads you want to assemble into transcripts"/>
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
148 </when>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
149 <when value="long_reads">
21
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
150 <param name="input_bam" type="data" format="sam,bam,cram" label="Input long mapped reads" help="Input BAM/SAM/CRAM file containing the long reads you want to assemble into transcripts"/>
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
151 <param name="error_splice" argument="-E" type="integer" min="0" max="50" value="25" label="Window around possibly erroneous splice sites" help="This option allows to define window around possibly erroneous splice sites from long reads to look out for correct splice sites. Default: 25" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
152 </when>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
153 <when value="mixed_reads">
21
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
154 <param name="input_bam_short" type="data" format="sam,bam,cram" label="Input short mapped reads" help="Input BAM/SAM/CRAM file containing the short reads you want to assemble into transcripts"/>
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
155 <param name="input_bam_long" type="data" format="sam,bam,cram" label="Input long mapped reads" help="Input BAM/SAM/CRAM file containing the long reads you want to assemble into transcripts"/>
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
156 <param name="error_splice" argument="-E" type="integer" min="0" max="50" value="25" label="Window around possibly erroneous splice sites" help="This option allows to define window around possibly erroneous splice sites from long reads to look out for correct splice sites. Default: 25" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
157 </when>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
158 </conditional>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
159 <param name="rna_strandness" type="select" label="Specify strand information"
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
160 help="Select 'Forward (FR)' if your reads are from a forward-stranded library, 'Reverse (RF)' if your reads are from a reverse-stranded library, or 'Unstranded' if your reads are not from a stranded library. See Help section below for more information. Default: Unstranded">
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
161 <option value="" selected="true">Unstranded</option>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
162 <option value="--fr">Forward (FR)</option>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
163 <option value="--rf">Reverse (RF)</option>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
164 </param>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
165 <conditional name="guide">
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
166 <param name="use_guide" argument="-G" type="select" label="Use a reference file to guide assembly?" help="Use the reference annotation file (in GTF or GFF3 format) to guide the assembly process. The output will include expressed reference transcripts as well as any novel transcripts that are assembled. This option is required by option -e (Use Reference transcripts only), see below.">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
167 <option value="yes">Use reference GTF/GFF3</option>
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
168 <option value="no" selected="true" >Do not use reference GTF/GFF3</option>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
169 </param>
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
170 <when value="no" />
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
171 <when value="yes">
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
172 <conditional name="guide_source">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
173 <param name="guide_gff_select" type="select" label="Reference file">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
174 <option value="cached" selected="true">Use a built-in file</option>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
175 <option value="history">Use a file from history</option>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
176 </param>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
177 <when value="cached">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
178 <param name="ref_builtin" type="select" label="Use a built-in GTF" help="If the GTF file for your transcriptome of interest is not listed, contact your Galaxy administrator">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
179 <options from_data_table="gene_sets">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
180 <filter type="sort_by" column="2" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
181 <validator type="no_options" message="No GTF file is available." />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
182 </options>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
183 </param>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
184 </when>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
185 <when value="history">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
186 <param name="ref_hist" type="data" format="gtf,gff3" label="GTF/GFF3 dataset to guide assembly" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
187 </when>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
188 </conditional>
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
189 <param name="input_estimation" argument="-e" type="boolean" truevalue="-e" falsevalue="" checked="false" label="Use Reference transcripts only?" help="Limit the processing of read alignments to only estimate and output the assembled transcripts matching the reference transcripts given with the -G option. With this option, read bundles with no reference transcripts (novel transcripts) will be entirely skipped, which may provide a considerable speed boost when the given set of reference transcripts is limited to a set of target genes, for example. Default: No"/>
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
190 <conditional name="special_outputs">
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
191 <param name="special_outputs_select" type="select" label="Output files for differential expression?" help="Select to output additional files that can be used with Ballgown or DESeq2/edgeR. See Help section below for more information">
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
192 <option value="ballgown">Ballgown</option>
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
193 <option value="deseq2">DESeq2/edgeR/limma-voom</option>
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
194 <option value="no" selected="true">No additional output</option>
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
195 </param>
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
196 <when value="ballgown" />
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
197 <when value="deseq2">
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
198 <param name="read_length" argument="--length" type="integer" min="0" value="75" label="Specify the average read length" help="Default: 75" />
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
199 <param name="clustering" argument="--cluster" type="boolean" truevalue="--cluster" falsevalue="" checked="false" label="Cluster overlapping genes" help="Choose whether to cluster genes with different gene IDs that overlap. Transcripts containing the geneID prefix will be ignored. Default: No" />
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
200 <param argument="--string" type="text" label="Prefix used for transcripts" help="If a different prefix was used for geneIDs assigned by StringTie than the default, specify it here. Only letters and numbers will be retained in this field. Default: MSTRG" >
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
201 <sanitizer>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
202 <valid initial="string.letters,string.digits"></valid>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
203 </sanitizer>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
204 </param>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
205 <param argument="--key" type="text" label="Prefix for clustering" help="If clustering, what prefix to use for geneIDs assigned by this script. Only letters and numbers will be retained in this field. Default: prepG">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
206 <sanitizer>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
207 <valid initial="string.letters,string.digits"></valid>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
208 </sanitizer>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
209 </param>
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
210 </when>
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
211 <when value="no" />
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
212 </conditional>
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
213 <param name="coverage_file" argument="-C" type="boolean" truevalue="-C" falsevalue="" checked="false" label="Output coverage file?" help="If StringTie is run with this option (requires -G), it returns a file with all the transcripts in the reference annotation that are fully covered, end to end, by reads. The output format is a GTF file as described below. Each line of the GTF corresponds to a gene or transcript in the reference annotation."/>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
214 </when>
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
215 </conditional>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
216 <section name="adv" title="Advanced Options">
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
217 <param name="abundance_estimation" argument="-A" type="boolean" truevalue="-A" falsevalue="" checked="false" label="Output gene abundance estimation file?" help="If selected, gene abundances will be reported in a tab-delimited file, see below for more information"/>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
218 <param name="omit_sequences" argument="-x" type="text" value="" label="Do not assemble any transcripts on these reference sequence(s)" help="Ignore all read alignments (and thus do not attempt to perform transcript assembly) on the specified reference sequences. This parameter can be a single reference sequence name (e.g. chrM) or a comma-delimited list of sequence names (e.g. chrM,chrX,chrY). This can speed up StringTie especially in the case of excluding the mitochondrial genome, whose genes may have very high coverage in some cases, even though they may be of no interest for a particular RNA-Seq analysis. The reference sequence names are case sensitive, they must match identically the names of chromosomes/contigs of the target genome against which the RNA-Seq reads were aligned in the first place." />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
219 <param name="name_prefix" argument="-l" type="text" label="Name prefix for output transcripts" help="This prefix will be added to the name of the transcripts that are output. Only letters and numbers will be retained in this field. Default: STRG">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
220 <sanitizer>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
221 <valid initial="string.letters,string.digits"></valid>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
222 </sanitizer>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
223 </param>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
224 <param name="fraction" argument="-f" type="float" min="0.0" max="1.0" value="0.15" label="Minimum isoform fraction" help="Sets the minimum isoform abundance of the predicted transcripts as a fraction of the most abundant transcript assembled at a given locus. Lower abundance transcripts are often artifacts of incompletely spliced precursors of processed transcripts. Default: 0.15"/>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
225 <param name="min_tlen" argument="-m" type="integer" min="0" value="200" label="Minimum assembled transcript length" help="Sets the minimum length allowed for the predicted transcripts. Default: 200"/>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
226 <param name="min_anchor_len" argument="-a" type="integer" min="0" value="10" label="Minimum anchor length for junctions" help="Junctions that don't have spliced reads that align across them with at least this amount of bases on both sides are filtered out. Default: 10" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
227 <param name="min_anchor_cov" argument="-j" type="integer" min="0" value="1" label="Minimum junction coverage" help="There should be at least this many spliced reads that align across a junction (i.e. junction coverage). This number can be fractional, since some reads align in more than one place. A read that aligns in n places will contribute 1/n to the junction coverage. Default: 1" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
228 <param name="min_bundle_cov" argument="-c" type="integer" min="0" value="2" label="Minimum bundle reads per bp coverage to consider for assembly" help="Sets the minimum read coverage allowed for the predicted transcripts. A transcript with a lower coverage than this value is not shown in the output. Default: 2"/>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
229 <param name="bdist" argument="-g" type="integer" min="0" value="50" label="Gap between read mappings triggering a new bundle" help="Minimum locus gap separation value. Reads that are mapped closer than this distance are merged together in the same processing bundle. Default: 50 (bp)"/>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
230 <param name="bundle_fraction" argument="-M" type="float" min="0.0" max="1.0" value="0.95" label="Fraction of bundle allowed to be covered by multi-hit reads" help="Sets the maximum fraction of muliple-location-mapped reads that are allowed to be present at a given locus. Default: 0.95"/>
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
231 <param name="disable_trimming" argument="-t" type="boolean" truevalue="-t" falsevalue="" checked="false" label="Disable trimming of predicted transcripts based on coverage" help="This parameter disables trimming at the ends of the assembled transcripts. By default StringTie adjusts the predicted transcript's start and/or stop coordinates based on sudden drops in coverage of the assembled transcript. Default: No" />
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
232 <param name="multi_mapping" argument="-u" type="boolean" truevalue="-u" falsevalue="" checked="false" label="Disable multi-mapping correction" help="Default: No"/>
19
333a6e13b622 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a586aaa6ef561527cda9e922a2260aa591f43938"
iuc
parents: 18
diff changeset
233 <param name="point_features" type="data" format="tabular" optional="true" label="Input point-features dataset" help="Loads a list of point-features from a text feature file to guide the transcriptome assembly. Accepted point features are transcription start sites (TSS) and polyadenylation sites (CPAS). There are four tab-delimited columns in the feature file. The first three define the location of the point feature on the cromosome (sequence name, coordinate and strand), and the last is the type of the feature (TSS or CPAS)."/>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
234 </section>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
235 </inputs>
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
236 <outputs>
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
237 <data name="output_gtf" format="gtf" label="${tool.name} on ${on_string}: Assembled transcripts" />
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
238 <data name="gene_abundance_estimation" format="tabular" label="${tool.name} on ${on_string}: Gene abundance estimates">
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
239 <filter>adv['abundance_estimation']</filter>
5
f504b3b7e49d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 65c9ce2fdc2b4ce892df8e456f18b859a85c410a
iuc
parents: 4
diff changeset
240 </data>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
241 <data name="coverage" format="gtf" label="${tool.name} on ${on_string}: Coverage">
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
242 <filter>guide['use_guide'] == 'yes' and guide['coverage_file'] is True </filter>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
243 </data>
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
244 <data name="exon_expression" format="tabular" from_work_dir="special_de_output/sample1/e_data.ctab"
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
245 label="${tool.name} on ${on_string}: exon-level expression measurements">
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
246 <filter>guide['use_guide'] == 'yes' and guide['special_outputs']['special_outputs_select'] == 'ballgown'</filter>
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
247 </data>
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
248 <data name="intron_expression" format="tabular" from_work_dir="special_de_output/sample1/i_data.ctab"
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
249 label="${tool.name} on ${on_string}: intron-level expression measurements">
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
250 <filter>guide['use_guide'] == 'yes' and guide['special_outputs']['special_outputs_select'] == 'ballgown'</filter>
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
251 </data>
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
252 <data name="transcript_expression" format="tabular" from_work_dir="special_de_output/sample1/t_data.ctab"
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
253 label="${tool.name} on ${on_string}: transcript-level expression measurements">
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
254 <filter>guide['use_guide'] == 'yes' and guide['special_outputs']['special_outputs_select'] == 'ballgown'</filter>
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
255 </data>
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
256 <data name="exon_transcript_mapping" format="tabular" from_work_dir="special_de_output/sample1/e2t.ctab"
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
257 label="${tool.name} on ${on_string}: exon to transcript mapping">
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
258 <filter>guide['use_guide'] == 'yes' and guide['special_outputs']['special_outputs_select'] == 'ballgown'</filter>
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
259 </data>
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
260 <data name="intron_transcript_mapping" format="tabular" from_work_dir="special_de_output/sample1/i2t.ctab"
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
261 label="${tool.name} on ${on_string}: intron to transcript mapping">
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
262 <filter>guide['use_guide'] == 'yes' and guide['special_outputs']['special_outputs_select'] == 'ballgown'</filter>
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
263 </data>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
264 <data name="gene_counts" format="tabular" from_work_dir="special_de_output/sample1/gene_counts.tsv" label="${tool.name} on ${on_string}: Gene counts">
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
265 <filter>guide['use_guide'] == 'yes' and guide['special_outputs']['special_outputs_select'] == 'deseq2'</filter>
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
266 </data>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
267 <data name="transcript_counts" format="tabular" from_work_dir="special_de_output/sample1/transcript_counts.tsv" label="${tool.name} on ${on_string}: Transcript counts">
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
268 <filter>guide['use_guide'] == 'yes' and guide['special_outputs']['special_outputs_select'] == 'deseq2'</filter>
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
269 </data>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
270 <data name="legend" format="tabular" from_work_dir="special_de_output/sample1/legend.tsv" label="${tool.name} on ${on_string}: legend">
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
271 <filter>guide['use_guide'] == 'yes' and guide['special_outputs']['special_outputs_select'] == 'deseq2' and guide['special_outputs']['clustering'] is True</filter>
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
272 </data>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
273 </outputs>
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
274 <tests>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
275 <!--Ensure default GTF output works -->
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
276 <test expect_num_outputs="1">
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
277 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
278 <param name="input_mode" value="short_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
279 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
280 </conditional>
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
281 <output name="output_gtf" file="stringtie_out1_re.gtf" ftype="gtf" compare="re_match" sort="true"/>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
282 </test>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
283 <!--Ensure fraction option works -->
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
284 <test expect_num_outputs="1">
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
285 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
286 <param name="input_mode" value="short_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
287 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
288 </conditional>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
289 <param name="fraction" value="0.17" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
290 <output name="output_gtf" file="stringtie_out2_re.gtf" ftype="gtf" compare="re_match" sort="true"/>
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
291 </test>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
292 <!--Ensure guide option works -->
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
293 <test expect_num_outputs="1">
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
294 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
295 <param name="input_mode" value="short_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
296 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
297 </conditional>
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
298 <param name="use_guide" value="yes" />
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
299 <param name="guide_gff_select" value="history" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
300 <param name="ref_hist" ftype="gtf" value="stringtie_in.gtf" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
301 <output name="output_gtf" file="stringtie_out3_re.gtf" ftype="gtf" compare="re_match" sort="true"/>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
302 </test>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
303 <!--Ensure guide with fraction works -->
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
304 <test expect_num_outputs="1">
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
305 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
306 <param name="input_mode" value="short_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
307 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
308 </conditional>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
309 <param name="use_guide" value="yes" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
310 <param name="guide_gff_select" value="history" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
311 <param name="ref_hist" ftype="gtf" value="stringtie_in.gtf" />
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
312 <param name="fraction" value="0.17" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
313 <output name="output_gtf" file="stringtie_out4_re.gtf" ftype="gtf" compare="re_match" sort="true"/>
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
314 </test>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
315 <!--Ensure coverage and output for Ballgown works -->
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
316 <test expect_num_outputs="7">
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
317 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
318 <param name="input_mode" value="short_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
319 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
320 </conditional>
1
9f80c71f1779 Uploaded updated tool wrapper for stringtie 1.0.1
iuc
parents: 0
diff changeset
321 <param name="use_guide" value="yes" />
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
322 <param name="guide_gff_select" value="history" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
323 <param name="ref_hist" ftype="gtf" value="stringtie_in.gtf" />
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
324 <param name="special_outputs_select" value="ballgown" />
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
325 <param name="coverage_file" value="true" />
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
326 <output name="exon_expression" file="./ballgown/e_data.ctab" ftype="tabular" />
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
327 <output name="intron_expression" file="./ballgown/i_data.ctab" ftype="tabular" />
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
328 <output name="transcript_expression" file="./ballgown/t_data.ctab" ftype="tabular" />
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
329 <output name="exon_transcript_mapping" file="./ballgown/e2t.ctab" ftype="tabular" />
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
330 <output name="intron_transcript_mapping" file="./ballgown/i2t.ctab" ftype="tabular" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
331 <output name="output_gtf" file="stringtie_out5.gtf" ftype="gtf" compare="re_match" sort="true"/>
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
332 <output name="coverage" file="stringtie_out_coverage.gtf" ftype="gtf" compare="re_match" sort="true"/>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
333 </test>
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
334 <!--Ensure output for edgeR works -->
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
335 <test expect_num_outputs="5">
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
336 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
337 <param name="input_mode" value="short_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
338 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
339 </conditional>
5
f504b3b7e49d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 65c9ce2fdc2b4ce892df8e456f18b859a85c410a
iuc
parents: 4
diff changeset
340 <param name="use_guide" value="yes" />
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
341 <param name="special_outputs_select" value="deseq2" />
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
342 <param name="input_estimation" value="true" />
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
343 <param name="guide_gff_select" value="history" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
344 <param name="ref_hist" ftype="gtf" value="stringtie_in.gtf" />
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
345 <param name="coverage_file" value="true" />
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
346 <param name="clustering" value="true" />
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
347 <output name="gene_counts" file="gene_counts_edger.tsv" ftype="tabular" />
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
348 <output name="transcript_counts" file="transcript_counts_edger.tsv" ftype="tabular" />
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
349 <output name="legend" file="legend.tsv" ftype="tabular" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
350 <output name="output_gtf" file="stringtie_out6.gtf" ftype="gtf" compare="re_match" sort="true"/>
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
351 <output name="coverage" file="stringtie_out_coverage.gtf" ftype="gtf" compare="re_match" sort="true"/>
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
352 </test>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
353 <!--Ensure gene abundances output works -->
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
354 <test expect_num_outputs="2">
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
355 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
356 <param name="input_mode" value="short_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
357 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
358 </conditional>
10
c84d44519b2e planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b78c073ab258852730fc9af1cd4862d571459103
iuc
parents: 7
diff changeset
359 <param name="use_guide" value="yes" />
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
360 <param name="guide_gff_select" value="history" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
361 <param name="ref_hist" ftype="gtf" value="stringtie_in.gtf" />
5
f504b3b7e49d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 65c9ce2fdc2b4ce892df8e456f18b859a85c410a
iuc
parents: 4
diff changeset
362 <param name="fraction" value="0.17" />
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
363 <param name="abundance_estimation" value="true" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
364 <output name="output_gtf" file="stringtie_out4_re.gtf" ftype="gtf" compare="re_match" sort="true"/>
16
eba36e001f45 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7dbb0fbe5446140f40fed36e45f42ec8d34bbc78
iuc
parents: 15
diff changeset
365 <output name="gene_abundance_estimation" file="stringtie_out7.tsv" ftype="tabular" lines_diff="2" />
5
f504b3b7e49d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 65c9ce2fdc2b4ce892df8e456f18b859a85c410a
iuc
parents: 4
diff changeset
366 </test>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
367 <!--Ensure another fraction value works -->
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
368 <test expect_num_outputs="1">
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
369 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
370 <param name="input_mode" value="short_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
371 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
372 </conditional>
5
f504b3b7e49d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 65c9ce2fdc2b4ce892df8e456f18b859a85c410a
iuc
parents: 4
diff changeset
373 <param name="use_guide" value="yes" />
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
374 <param name="guide_gff_select" value="history" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
375 <param name="ref_hist" ftype="gtf" value="stringtie_in.gtf" />
5
f504b3b7e49d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 65c9ce2fdc2b4ce892df8e456f18b859a85c410a
iuc
parents: 4
diff changeset
376 <param name="fraction" value="0.15" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
377 <output name="output_gtf" file="stringtie_out8.gtf" ftype="gtf" compare="re_match" sort="true"/>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
378 </test>
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
379 <!--Ensure built-in GTFs work -->
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
380 <test expect_num_outputs="1">
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
381 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
382 <param name="input_mode" value="short_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
383 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
384 </conditional>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
385 <param name="use_guide" value="yes" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
386 <param name="guide_gff_select" value="cached" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
387 <param name="fraction" value="0.15" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
388 <output name="output_gtf" file="stringtie_out9.gtf" ftype="gtf" compare="re_match" sort="true"/>
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
389 </test>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
390 <!-- Test long reads input -->
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
391 <test expect_num_outputs="1">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
392 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
393 <param name="input_mode" value="long_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
394 <param name="input_bam" ftype="bam" value="stringtie_in1.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
395 </conditional>
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
396 <param name="use_guide" value="yes" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
397 <param name="guide_gff_select" value="cached" />
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
398 <param name="fraction" value="0.15" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
399 <output name="output_gtf" file="stringtie_out10.gtf" ftype="gtf" compare="re_match" sort="true"/>
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
400 </test>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
401 <!-- Test error splice option -->
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
402 <test expect_num_outputs="1">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
403 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
404 <param name="input_mode" value="long_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
405 <param name="input_bam" ftype="bam" value="long_reads.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
406 <param name="error_splice" value="30" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
407 </conditional>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
408 <param name="use_guide" value="yes" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
409 <param name="guide_gff_select" value="cached" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
410 <param name="fraction" value="0.15" />
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
411 <output name="output_gtf" file="stringtie_out11.gtf" ftype="gtf" compare="re_match" sort="true"/>
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
412 </test>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
413 <!-- Test mixed reads input -->
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
414 <test expect_num_outputs="1">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
415 <conditional name="input_options">
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
416 <param name="input_mode" value="mixed_reads"/>
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
417 <param name="input_bam_short" ftype="bam" value="short_reads.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
418 <param name="input_bam_long" ftype="bam" value="long_reads.bam" />
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
419 </conditional>
20
cef79931dda5 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 7f649c10c5d874f466122af0d24803a4c6affabb
iuc
parents: 19
diff changeset
420 <output name="output_gtf" file="stringtie_out12.gtf" ftype="gtf" compare="re_match" sort="true"/>
5
f504b3b7e49d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 65c9ce2fdc2b4ce892df8e456f18b859a85c410a
iuc
parents: 4
diff changeset
421 </test>
21
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
422 <!-- Test cram input -->
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
423 <test expect_num_outputs="1">
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
424 <conditional name="input_options">
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
425 <param name="input_mode" value="mixed_reads"/>
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
426 <param name="input_bam_short" ftype="cram" value="stringtie_in.cram" />
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
427 <param name="input_bam_long" ftype="cram" value="stringtie_in.cram" />
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
428 </conditional>
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
429 <output name="output_gtf" ftype="gtf">
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
430 <assert_contents>
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
431 <has_text text="gi|251831106|ref|NC_012920.1|"/>
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
432 <has_text text="StringTie version 2.2.1"/>
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
433 </assert_contents>
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
434 </output>
914cfcba0ec8 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 8923485156dc1150213ad1f324aa16aaef4ae643
iuc
parents: 20
diff changeset
435 </test>
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
436 </tests>
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
437 <help><![CDATA[
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
438
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
439 .. class:: infomark
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
440
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
441 **What it does**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
442
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
443 StringTie_ is a fast and highly efficient assembler of RNA-Seq alignments into potential transcripts. It uses a novel network flow algorithm as well as an optional *de novo* assembly step to assemble and quantitate full-length transcripts representing multiple splice variants for each gene locus. Its input can include not only the alignments of raw reads used by other transcript assemblers, but also alignments of longer sequences that have been assembled from those reads. In order to identify differentially expressed genes between experiments, StringTie's output can be processed by specialized software like Ballgown_, Cuffdiff_ or other programs (DESeq2_, edgeR_, limma_ etc.).
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
444
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
445 -----
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
446
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
447 **Inputs**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
448
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
449 StringTie takes as input a BAM (or SAM) file of paired-end RNA-seq reads, which must be sorted by genomic location (coordinate position). This file contains spliced read alignments and can be produced directly by programs such as HISAT2_. We recommend using HISAT2 as it is a fast and accurate alignment program. Every spliced read alignment (i.e. an alignment across at least one junction) in the input BAM file must contain the tag XS to indicate the genomic strand that produced the RNA from which the read was sequenced. Alignments produced by HISAT2 (when run with --dta option) already include this tag, but if you use a different read mapper you should check that this XS tag is included for spliced alignments.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
450
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
451 *NOTE: be sure to run HISAT2 with the --dta option for alignment (under Spliced alignment options), or your results will suffer.*
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
452
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
453 Also note that if your reads are from a stranded library, you need to choose the appropriate setting under **Specify strand information** above. As, if Forward (FR) is selected, StringTie will assume the reads are from a --fr library, while if Reverse (RF) is selected, StringTie will assume the reads are from a --rf library, otherwise it is assumed that the reads are from an unstranded library (The widely-used, although now deprecated, TopHat had a similar --library-type option, where fr-firststrand corresponded to RF; fr-secondstrand corresponded to FR). If you don't know whether your reads are from are a stranded library or not, you could use the tool **RSeQC Infer Experiment** to try to determine.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
454
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
455 As an option, a reference annotation file in `GTF/GFF3`_ format can be provided to StringTie. In this case, StringTie will prefer to use these "known" genes from the annotation file, and for the ones that are expressed it will compute coverage, TPM and FPKM values. It will also produce additional transcripts to account for RNA-seq data that aren't covered by (or explained by) the annotation. Note that if option -e is not used the reference transcripts need to be fully covered by reads in order to be included in StringTie's output. In that case, other transcripts assembled from the data by StringTie and not present in the reference file will be printed as well.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
456
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
457 *NOTE: we highly recommend that you provide annotation if you are analyzing a genome that is well-annotated, such as human, mouse, or other model organisms.*
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
458
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
459 -----
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
460
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
461 **Outputs**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
462
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
463 StringTie's primary output is
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
464
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
465 * a GTF file containing the **Assembled transcripts**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
466
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
467 Optionally, it can output
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
468
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
469 * a TSV (tab-delimited) file of **Gene abundances**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
470
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
471 If a reference GTF/GFF3 file is used as a guide, StringTie can also output:
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
472
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
473 * a GTF file containing all **fully-covered reference transcripts** in the provided reference file that are covered end-to-end by reads
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
474 * Files (tables) for **Ballgown** and/or **DESeq2/edgeR/limma-voom**, which can use them to estimate differential expression
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
475
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
476
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
477 **StringTie's primary GTF output**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
478
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
479 The primary output of StringTie is a Gene Transfer Format (GTF) file that contains details of the transcripts that StringTie assembles from RNA-Seq data. GTF is an extension of GFF (Gene Finding Format, also called General Feature Format), and is very similar to GFF2 and GFF3. The field definitions for the 9 columns of GTF output can be found at the `Ensembl site here`_. The following is an example of a transcript assembled by StringTie as shown in a GTF file:
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
480
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
481 =========== ========== =========== ========= ======= ========= ========== ========= ===========================================================================================
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
482 **seqname** **source** **feature** **start** **end** **score** **strand** **frame** **attributes**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
483 ----------- ---------- ----------- --------- ------- --------- ---------- --------- -------------------------------------------------------------------------------------------
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
484 chrX StringTie transcript 281394 303355 1000 \+ . gene_id "ERR188044.1"; transcript_id "ERR188044.1.1"; reference_id "NM_018390"; ref_gene_id
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
485 "NM_018390"; ref_gene_name "PLCXD1"; cov "101.256691"; FPKM "530.078918"; TPM "705.667908";
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
486 chrX StringTie exon 281394 281684 1000 \+ . gene_id "ERR188044.1"; transcript_id "ERR188044.1.1"; exon_number "1"; reference_id
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
487 "NM_018390"; ref_gene_id "NM_018390"; ref_gene_name "PLCXD1"; cov "116.270836";
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
488 =========== ========== =========== ========= ======= ========= ========== ========= ===========================================================================================
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
489
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
490
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
491 * **seqname**: Denotes the chromosome, contig, or scaffold for this transcript. Here the assembled transcript is on chromosome X.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
492
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
493 * **source**: The source of the GTF file. Since this example was produced by StringTie, this column simply shows 'StringTie'.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
494
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
495 * **feature**: Feature type (e.g., exon, transcript, mRNA, 5'UTR).
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
496
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
497 * **start**: Start position of the feature (exon, transcript, etc), using a 1-based index.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
498
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
499 * **end**: End position of the feature, using a 1-based index.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
500
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
501 * **score**: A confidence score for the assembled transcript. Currently this field is not used, and StringTie reports a constant value of 1000 if the transcript has a connection to a read alignment bundle.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
502
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
503 * **strand**: If the transcript resides on the forward strand, '+'. If the transcript resides on the reverse strand, '-'.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
504
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
505 * **frame**: Frame or phase of CDS features. StringTie does not use this field and simply records a ".".
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
506
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
507 * **attributes**: A semicolon-separated list of tag-value pairs, providing additional information about each feature. Depending on whether an instance is a transcript or an exon and on whether the transcript matches the reference annotation file provided by the user, the content of the attributes field will differ. The following list describes the possible attributes shown in this column:
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
508
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
509 * *gene_id*: A unique identifier for a single gene and its child transcript and exons based on the alignments' file name.
2
520e0988ec1c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b03fd81ed11744f119608d4884c24fa182eb605a
iuc
parents: 1
diff changeset
510
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
511 * *transcript_id*: A unique identifier for a single transcript and its child exons based on the alignments' file name.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
512
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
513 * *exon_number*: A unique identifier for a single exon, starting from 1, within a given transcript.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
514
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
515 * *reference_id*: The transcript_id in the reference annotation (optional) that the instance matched.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
516
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
517 * *ref_gene_id*: The gene_id in the reference annotation (optional) that the instance matched.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
518
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
519 * *ref_gene_name*: The gene_name in the reference annotation (optional) that the instance matched.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
520
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
521 * *cov*: The average per-base coverage for the transcript or exon.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
522
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
523 * *FPKM*: Fragments per kilobase of transcript per million read pairs. This is the number of pairs of reads aligning to this feature, normalized by the total number of fragments sequenced (in millions) and the length of the transcript (in kilobases).
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
524
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
525 * *TPM*: Transcripts per million. This is the number of transcripts from this particular gene normalized first by gene length, and then by sequencing depth (in millions) in the sample. A detailed explanation and a comparison of TPM and FPKM can be found here_, and TPM was defined `by B. Li and C. Dewey here`_.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
526
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
527
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
528 **Gene abundances in tab-delimited format**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
529
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
530 If StringTie is run with the -A option, it returns a file containing gene abundances. The tab-delimited gene abundances output file has nine fields; below is an example of a gene abundance file produced by StringTie using reference annotation:
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
531
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
532 =========== ============= ============= ========== ========= ======= ============ ======== ========
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
533 **Gene ID** **Gene Name** **Reference** **Strand** **Start** **End** **Coverage** **FPKM** **TPM**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
534 ----------- ------------- ------------- ---------- --------- ------- ------------ -------- --------
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
535 NM_000451 SHOX chrX \+ 624344 646823 0.000000 0.000000 0.000000
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
536 NM_006883 SHOX chrX \+ 624344 659411 0.000000 0.000000 0.000000
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
537 =========== ============= ============= ========== ========= ======= ============ ======== ========
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
538
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
539 * **Gene ID**: The gene identifier comes from the reference annotation provided with the -G option. If no reference is provided this field is replaced with the name prefix for output transcripts (-l).
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
540 * **Gene Name**: This field contains the gene name in the reference annotation provided with the -G option. If no reference is provided this field is populated with '-'.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
541 * **Reference**: Name of the reference sequence that was used in the alignment of the reads. Equivalent to the 3rd column in the .SAM alignment.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
542 * **Strand**: '+' denotes that the gene is on the forward strand, '-' for the reverse strand.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
543 * **Start**: Start position of the gene (1-based index).
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
544 * **End**: End position of the gene (1-based index).
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
545 * **Coverage**: Per-base coverage of the gene.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
546 * **FPKM**: normalized expression level in FPKM units (see previous section).
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
547 * **TPM**: normalized expression level in RPM units (see previous section).
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
548
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
549 **Fully covered transcripts matching the reference annotation transcripts (in GTF format)**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
550
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
551 If StringTie is run with the use reference guide option (-G), it will also return a file with all the transcripts in the reference annotation that are fully covered, end to end, by reads. The output format is a GTF file as described above. Each line of the GTF is corresponds to a gene or transcript in the reference annotation.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
552
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
553 **Ballgown Input Table Files**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
554
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
555 An option to output files for Ballgown can be selected under **Output files for differential expression?** above. If selected, StringTie will return Ballgown input table files containing coverage data for the reference transcripts given with the -G option. These tables have these specific names: (1) e2t.ctab, (2) e_data.ctab, (3) i2t.ctab, (4) i_data.ctab, and (5) t_data.ctab. A detailed description of each of these five required inputs to Ballgown can be found at `this link`. With this option StringTie can be used as a direct replacement of the tablemaker program included with the Ballgown distribution.
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
556
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
557
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
558 **DESeq2/edgeR/limma-voom Input Table Files**
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
559
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
560 DESeq2_, edgeR_ and limma_ are three popular Bioconductor_ packages for analyzing differential expression, which take as input a matrix of read counts mapped to particular genomic features (e.g., genes). This read count information can be extracted directly from the files generated by StringTie (run with the -e parameter) by selecting DESeq2/edgeR/limma-voom under **Output files for differential expression?** above. This uses the StringTie helper script ``prepDE.py`` to convert the GTF output from StringTie into two tab-delimited (TSV) files, containing the count matrices for genes and transcripts, using the coverage values found in the output of StringTie -e.
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
561
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
562 -----
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
563
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
564 **More Information**
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
565
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
566 *Evaluating transcript assemblies:*
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
567 A simple way of getting more information about the transcripts assembled by StringTie (summary of gene and transcript counts, novel vs. known etc.), or even performing basic tracking of assembled isoforms across multiple RNA-Seq experiments, is to use the **gffcompare** program. Basic usage information for this program can be found on the `GFF utilities page`_.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
568
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
569 *Differential expression analysis:*
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
570
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
571 Together with HISAT and Ballgown (or DESeq2/edgeR/limma-voom), StringTie can be used for estimating differential expression across multiple RNA-Seq samples and generating plots and differential expression tables as described in our `protocol paper`_ and shown in a diagram in the `StringTie manual here`_.
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
572
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
573 Our recommended workflow includes the following steps:
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
574
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
575 1. For each RNA-Seq sample, map the reads to the genome with HISAT2 using the --dta option. It is highly recommended to use the reference annotation information when mapping the reads, which can be either embedded in the genome index (built with the --ss and --exon options, see HISAT2 manual), or provided separately at run time (using the --known-splicesite-infile option of HISAT2). The SAM output of each HISAT2 run must be sorted and converted to BAM using samtools as explained above.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
576
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
577 2. For each RNA-Seq sample, use this StringTie tool to assemble the read alignments obtained in the previous step; it is recommended to run StringTie with the -G option if the reference annotation is available.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
578
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
579 3. Run the separate **StringTie merge** tool in order to generate a non-redundant set of transcripts observed in all the RNA-Seq samples assembled previously. ``StringTie merge`` takes as input a list of all the assembled transcripts files (in GTF format) previously obtained for each sample, as well as a reference annotation file (-G option) if available.
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
580
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
581 4. For each RNA-Seq sample, run this StringTie tool selecting to output files for Ballgown (or DESeq2/edgeR/limma-voom), which will generate tables of transcript and gene estimated abundances (count files). The option -e (*Use Reference transcripts only*) is not required but is recommended for this run in order to produce more accurate abundance estimations of the input transcripts. Each StringTie run in this step will take as input the sorted read alignments (BAM file) obtained in step 1 for the corresponding sample and the -G option with the merged transcripts (GTF file) generated by ``stringtie merge`` in step 3. Please note that this is the only case where the -G option is not used with a reference annotation, but with the global, merged set of transcripts as observed across all samples. (This step is the equivalent of the *Tablemaker* step described in the original Ballgown pipeline.)
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
582
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
583 5. Ballgown (or DESeq2/edgeR/limma-voom) can now be used to load the coverage tables generated in the previous step and perform various statistical analyses for differential expression, generate plots etc.
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
584
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
585 An alternate, faster differential expression analysis workflow can be pursued if there is no interest in novel isoforms (i.e. assembled transcripts present in the samples but missing from the reference annotation), or if only a well known set of transcripts of interest are targeted by the analysis. This simplified protocol has only 3 steps (depicted in the `StringTie manual here`_) as it bypasses the individual assembly of each RNA-Seq sample and the "transcript merge" step. This simplified workflow attempts to directly estimate and analyze the expression of a known set of transcripts as given in the reference annotation file.
0
62d212192002 Imported from capsule None
iuc
parents:
diff changeset
586
2
520e0988ec1c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit b03fd81ed11744f119608d4884c24fa182eb605a
iuc
parents: 1
diff changeset
587 .. _StringTie: http://ccb.jhu.edu/software/stringtie/
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
588 .. _Ballgown: https://www.biorxiv.org/content/early/2014/09/05/003665
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
589 .. _Cuffdiff: http://cole-trapnell-lab.github.io/cufflinks/cuffdiff/
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
590 .. _DESeq2: https://bioconductor.org/packages/release/bioc/html/DESeq2.html
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
591 .. _edgeR: https://bioconductor.org/packages/release/bioc/html/edgeR.html
15
dd4df992d93d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit a834a41c94d184df80e45ffa2339723826a075b1
iuc
parents: 14
diff changeset
592 .. _limma: https://bioconductor.org/packages/release/bioc/html/limma.html
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
593 .. _Bioconductor: https://www.bioconductor.org/
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
594 .. _SAM: http://samtools.github.io/hts-specs/SAMv1.pdf
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
595 .. _HISAT2: http://ccb.jhu.edu/software/hisat2
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
596 .. _`GTF/GFF3`: http://ccb.jhu.edu/software/stringtie/gff.shtml
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
597 .. _`this link`: https://github.com/alyssafrazee/ballgown#ballgown-readable-expression-output
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
598 .. _`Ensembl site here`: http://useast.ensembl.org/info/website/upload/gff.html
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
599 .. _here: http://www.rna-seqblog.com/rpkm-fpkm-and-tpm-clearly-explained/
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
600 .. _`by B. Li and C. Dewey here`: http://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-12-323
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
601 .. _`GFF utilities page`: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffcompare
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
602 .. _`protocol paper`: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5032908/
18
258d696dbd7e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 999b45e8bcf810f871cda58cb66573f7d6ae37f3"
iuc
parents: 17
diff changeset
603 .. _`StringTie manual here`: http://ccb.jhu.edu/software/stringtie/index.shtml?t=manual
12
76d290331481 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit 11ee7ac206d41894c0b6a11f2439aaea490824f0
iuc
parents: 11
diff changeset
604
11
6e45b443ef1f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit d46e597732cda927a633e133c14dd4ece39f5edf
iuc
parents: 10
diff changeset
605 ]]></help>
6
d0bb240d7525 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit acdc3dfe5fd6317ae57899aa0e83130dff7460e7
iuc
parents: 5
diff changeset
606 <expand macro="citations" />
13
a305d75e13f2 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit e811a7887db870f4f94f620f52bce656c8d5ba23
iuc
parents: 12
diff changeset
607 </tool>