annotate structure.xml @ 1:64e681a1cad5 draft

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author iuc
date Wed, 06 Dec 2017 05:37:27 -0500
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1 <tool id="structure" name="Structure" version="2.3.4">
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2 <description>using multi-locus genotype data to investigate population structure</description>
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3 <requirements>
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4 <requirement type="package" version="2.3.4">structure</requirement>
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5 </requirements>
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6 <version_command><![CDATA[
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7 structure | grep -E -o 'Version.+'
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8 ]]></version_command>
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9 <command detect_errors="exit_code"><![CDATA[
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10 mv '$mainparams' '$out_mainparams' &&
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11 mv '$extraparams' '$out_extraparams' &&
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12
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13 mkdir out log
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15 #for $run in range(1, int($nb_run) + 1):
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16 && structure -i '$infile' -o outfile -m '$out_mainparams' -e '$out_extraparams' > 'log/run${run}_K_${main.MAXPOPS}.log'
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17 && mv 'outfile_f' 'out/run${run}_K_${main.MAXPOPS}.out'
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18 #end for
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20 ]]></command>
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21 <configfiles>
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22 <configfile name="mainparams"><![CDATA[
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23 KEY PARAMETERS FOR THE PROGRAM structure. YOU WILL NEED TO SET THESE
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24 IN ORDER TO RUN THE PROGRAM. VARIOUS OPTIONS CAN BE ADJUSTED IN THE
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25 FILE extraparams.
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26
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28 "(int)" means that this takes an integer value.
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29 "(B)" means that this variable is Boolean
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30 (ie insert 1 for True, and 0 for False)
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31 "(str)" means that this is a string (but not enclosed in quotes!)
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32
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33
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34 Basic Program Parameters
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35
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36 #define MAXPOPS $main.MAXPOPS // default:2 // (int) number of populations assumed
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37 #define BURNIN $main.BURNIN // default:10000 // (int) length of burnin period
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38 #define NUMREPS $main.NUMREPS // default:20000 // (int) number of MCMC reps after burnin
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39
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40 Input/Output files
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41
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42 #define INFILE $infile // (str) name of input data file
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43 #define OUTFILE outfile //(str) name of output data file
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44
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45 Data file format
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46
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47 #define NUMINDS $main.NUMINDS // default:100 // (int) number of diploid individuals in data file
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48 #define NUMLOCI $main.NUMLOCI // default:100 // (int) number of loci in data file
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49 #define PLOIDY $main.PLOIDY // default:2 // (int) ploidy of data
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50 #define MISSING $main.MISSING // default:-9 // (int) value given to missing genotype data
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51 #define ONEROWPERIND $main.ONEROWPERIND // default:0 // (B) store data for individuals in a single line
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52
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53
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54 #define LABEL $main.LABEL // default:1 // (B) Input file contains individual labels
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55 #define POPDATA $main.POPDATA // default:1 // (B) Input file contains a population identifier
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56 #define POPFLAG ${extra.usepopinfo_cond.POPFLAG} // default:0 // (B) Input file contains a flag which says
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57 whether to use popinfo when USEPOPINFO==1
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58 #define LOCDATA $main.LOCDATA // default:0 // (B) Input file contains a location identifier
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59
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60 #define PHENOTYPE $main.PHENOTYPE // default:0 // (B) Input file contains phenotype information
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61 #define EXTRACOLS $main.EXTRACOLS // default:0 // (int) Number of additional columns of data
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62 before the genotype data start.
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63
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64 #define MARKERNAMES $main.MARKERNAMES // default:1 // (B) data file contains row of marker names
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65 #define RECESSIVEALLELES $main.recessivealleles_cond.RECESSIVEALLELES // default:0 // (B) data file contains dominant markers (eg AFLPs)
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66 // and a row to indicate which alleles are recessive
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67 #define MAPDISTANCES $main.MAPDISTANCES // default:0 // (B) data file contains row of map distances
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68 // between loci
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69
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70
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71 Advanced data file options
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72
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73 #define PHASED $main.PHASED // default:0 // (B) Data are in correct phase (relevant for linkage model only)
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74 #define PHASEINFO $main.PHASEINFO // default:0 // (B) the data for each individual contains a line
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75 indicating phase (linkage model)
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76 #define MARKOVPHASE $main.MARKOVPHASE // default:0 // (B) the phase info follows a Markov model.
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77 #define NOTAMBIGUOUS $main.recessivealleles_cond.NOTAMBIGUOUS // default:-999 // (int) for use in some analyses of polyploid data
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78
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79
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80
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81 Command line options:
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82
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83 -m mainparams
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84 -e extraparams
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85 -s stratparams
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86 -K MAXPOPS
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87 -L NUMLOCI
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88 -N NUMINDS
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89 -i input file
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90 -o output file
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91 -D SEED
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92
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93 ]]></configfile>
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94 <configfile name="extraparams"><![CDATA[
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95 EXTRA PARAMS FOR THE PROGRAM structure. THESE PARAMETERS CONTROL HOW THE
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96 PROGRAM RUNS. ATTRIBUTES OF THE DATAFILE AS WELL AS K AND RUNLENGTH ARE
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97 SPECIFIED IN mainparams.
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98
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99 "(int)" means that this takes an integer value.
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100 "(d)" means that this is a double (ie, a Real number such as 3.14).
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101 "(B)" means that this variable is Boolean
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102 (ie insert 1 for True, and 0 for False).
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103
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104 PROGRAM OPTIONS
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105
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106 #define NOADMIX $extra.NOADMIX // default:0 // (B) Use no admixture model (0=admixture model, 1=no-admix)
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107 #define LINKAGE $extra.LINKAGE // default:0 // (B) Use the linkage model model
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108 #define USEPOPINFO $extra.usepopinfo_cond.USEPOPINFO // default:0 // (B) Use prior population information to pre-assign individuals
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109 to clusters
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110 #define LOCPRIOR $extra.LOCPRIOR // default:0 //(B) Use location information to improve weak data
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111
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112 #define FREQSCORR $extra.FREQSCORR // default:1 // (B) allele frequencies are correlated among pops
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113 #define ONEFST $extra.ONEFST // default:0 // (B) assume same value of Fst for all subpopulations.
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114
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115 #define INFERALPHA $extra.inferalpha_cond.INFERALPHA // default:1 // (B) Infer ALPHA (the admixture parameter)
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116 #define POPALPHAS $extra.POPALPHAS // default:0 // (B) Individual alpha for each population
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117 #define ALPHA $extra.inferalpha_cond.ALPHA // default:1.0 // (d) Dirichlet parameter for degree of admixture
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118 (this is the initial value if INFERALPHA==1).
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119
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120 #define INFERLAMBDA $extra.inferlambda_cond.INFERLAMBDA // default:0 // (B) Infer LAMBDA (the allele frequencies parameter)
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121 #define POPSPECIFICLAMBDA $extra.inferlambda_cond.POPSPECIFICLAMBDA // default:0 //(B) infer a separate lambda for each pop
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122 (only if INFERLAMBDA=1).
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123 #define LAMBDA $extra.LAMBDA // default:1.0 // (d) Dirichlet parameter for allele frequencies
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124
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125
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126
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127
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128 PRIORS
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129
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130 #define FPRIORMEAN $extra.FPRIORMEAN // default:0.01 // (d) Prior mean and SD of Fst for pops.
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131 #define FPRIORSD $extra.FPRIORSD // default:0.05 // (d) The prior is a Gamma distribution with these parameters
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132
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133 #define UNIFPRIORALPHA $extra.unifprioralpha_cond.UNIFPRIORALPHA // default:1 // (B) use a uniform prior for alpha;
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134 otherwise gamma prior
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135 #define ALPHAMAX $extra.ALPHAMAX // default:10.0 // (d) max value of alpha if uniform prior
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136 #define ALPHAPRIORA $extra.unifprioralpha_cond.ALPHAPRIORA // default:1.0 // (only if UNIFPRIORALPHA==0): alpha has a gamma
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137 prior with mean A*B, and
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138 #define ALPHAPRIORB $extra.unifprioralpha_cond.ALPHAPRIORB // default:2.0 // variance A*B^2.
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139
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140
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141 #define LOG10RMIN $extra.LOG10RMIN // default:-4.0 //(d) Log10 of minimum allowed value of r under linkage model
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142 #define LOG10RMAX $extra.LOG10RMAX // default:1.0 //(d) Log10 of maximum allowed value of r
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143 #define LOG10RPROPSD $extra.LOG10RPROPSD // default:0.1 //(d) standard deviation of log r in update
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144 #define LOG10RSTART $extra.LOG10RSTART // default:-2.0 //(d) initial value of log10 r
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145
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146
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147 USING PRIOR POPULATION INFO (USEPOPINFO)
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148
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149 #define GENSBACK $extra.GENSBACK // default:2 //(int) For use when inferring whether an indiv-
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150 idual is an immigrant, or has an immigrant an-
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151 cestor in the past GENSBACK generations. eg, if
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152 GENSBACK==2, it tests for immigrant ancestry
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153 back to grandparents.
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154 #define MIGRPRIOR $extra.usepopinfo_cond.MIGRPRIOR // default:0.01 //(d) prior prob that an individual is a migrant
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155 (used only when USEPOPINFO==1). This should
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156 be small, eg 0.01 or 0.1.
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157 #define PFROMPOPFLAGONLY $extra.PFROMPOPFLAGONLY // default:0 // (B) only use individuals with POPFLAG=1 to update P.
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158 This is to enable use of a reference set of
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159 individuals for clustering additional "test"
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160 individuals.
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161
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162 LOCPRIOR MODEL FOR USING LOCATION INFORMATION
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163
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164 #define LOCISPOP $extra.LOCISPOP // default:1 //(B) use POPDATA for location information
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165 #define LOCPRIORINIT $extra.LOCPRIORINIT // default:1.0 //(d) initial value for r, the location prior
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166 #define MAXLOCPRIOR $extra.MAXLOCPRIOR // default:20.0 //(d) max allowed value for r
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167
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168
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169
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170
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171 OUTPUT OPTIONS
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172
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173 #define PRINTNET $extra.PRINTNET // default:1 // (B) Print the "net nucleotide distance" to screen during the run
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174 #define PRINTLAMBDA $extra.PRINTLAMBDA // default:1 // (B) Print current value(s) of lambda to screen
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175 #define PRINTQSUM $extra.PRINTQSUM // default:1 // (B) Print summary of current population membership to screen
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176
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177 #define SITEBYSITE $extra.SITEBYSITE // default:0 // (B) whether or not to print site by site results.
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178 (Linkage model only) This is a large file!
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179 #define PRINTQHAT $extra.PRINTQHAT // default:0 // (B) Q-hat printed to a separate file. Turn this
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180 on before using STRAT.
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181 #define UPDATEFREQ $extra.UPDATEFREQ // default:100 // (int) frequency of printing update on the screen.
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182 Set automatically if this is 0.
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183 #define PRINTLIKES $extra.PRINTLIKES // default:0 // (B) print current likelihood to screen every rep
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184 #define INTERMEDSAVE $extra.INTERMEDSAVE // default:0 // (int) number of saves to file during run
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185
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186 #define ECHODATA $extra.ECHODATA // default:1 // (B) Print some of data file to screen to check
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187 that the data entry is correct.
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188 (NEXT 3 ARE FOR COLLECTING DISTRIBUTION OF Q:)
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189 #define ANCESTDIST $extra.ANCESTDIST // default:0 // (B) collect data about the distribution of an-
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190 cestry coefficients (Q) for each individual
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191 #define NUMBOXES $extra.NUMBOXES // default:1000 // (int) the distribution of Q values is stored as
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192 a histogram with this number of boxes.
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193 #define ANCESTPINT $extra.ANCESTPINT // default:0.90 // (d) the size of the displayed probability
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194 interval on Q (values between 0.0--1.0)
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195
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196
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197
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198 MISCELLANEOUS
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199
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200 #define COMPUTEPROB $extra.COMPUTEPROB // default:1 // (B) Estimate the probability of the Data under
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201 the model. This is used when choosing the
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202 best number of subpopulations.
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203 #define ADMBURNIN $extra.ADMBURNIN // default:500 // (int) [only relevant for linkage model]:
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204 Initial period of burnin with admixture model (see Readme)
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205 #define ALPHAPROPSD $extra.ALPHAPROPSD // default:0.025 // (d) SD of proposal for updating alpha
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206 #define STARTATPOPINFO $extra.STARTATPOPINFO // default:0 // Use given populations as the initial condition
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207 for population origins. (Need POPDATA==1). It
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208 is assumed that the PopData in the input file
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209 are between 1 and k where k<=MAXPOPS.
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210 #define RANDOMIZE $extra.randomize_cond.RANDOMIZE // default:1 // (B) use new random seed for each run
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211 #define SEED $extra.randomize_cond.SEED // default:2245 // (int) seed value for random number generator
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212 (must set RANDOMIZE=0)
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213 #define METROFREQ $extra.METROFREQ // default:10 // (int) Frequency of using Metropolis step to update
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214 Q under admixture model (ie use the metr. move every
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215 i steps). If this is set to 0, it is never used.
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216 (Proposal for each q^(i) sampled from prior. The
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217 goal is to improve mixing for small alpha.)
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218 #define REPORTHITRATE $extra.REPORTHITRATE // default:0 // (B) report hit rate if using METROFREQ
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219
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220 ]]></configfile>
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221 </configfiles>
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222 <inputs>
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223 <param name="infile" type="data" label="Genotype data" format="tabular" />
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224 <param name="nb_run" value="1" type="integer" label="Number of runs" min="1" max="10" help="Note that the runs are sequential. Please launch separate runs if it's too long" />
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225 <section name="main" title="mainparams" expanded="True">
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226 <!--Basic Program Parameters-->
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227 <param argument="MAXPOPS" value="" type="integer" label="Number of populations assumed" help="or [K]"/>
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228 <param argument="BURNIN" value="10000" type="integer" label="Length of burnin period" />
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229 <param argument="NUMREPS" value="20000" type="integer" label="Number of MCMC reps after burnin" />
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230
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231 <!--Data file format-->
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232 <param argument="NUMINDS" value="" type="integer" label="Number of diploid individuals in data file" help="or [N]"/>
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233 <param argument="NUMLOCI" value="" type="integer" label="Number of loci in data file" help="or [L]"/>
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234 <param argument="PLOIDY" value="2" type="integer" label="Ploidy of data" />
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235 <param argument="MISSING" value="-9" type="integer" label="Value given to missing genotype data" />
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236 <param argument="ONEROWPERIND" checked="False" type="boolean" label="Store data for individuals in a single line" truevalue="1" falsevalue="0" help=" E.g., for diploid data, this would mean that the two alleles for each locus are in consecutive order in the same row, rather than being arranged in the same column, in two consecutive rows "/>
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237
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238
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239 <param argument="LABEL" checked="true" type="boolean" label="Input file contains individual labels" truevalue="1" falsevalue="0" />
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240 <param argument="POPDATA" checked="true" type="boolean" label="Input file contains a user-defined population-of-origin for each individual" truevalue="1" falsevalue="0" />
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241 <param argument="LOCDATA" checked="false" type="boolean" label="Input file contains a location identifier" truevalue="1" falsevalue="0" />
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242
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243 <param argument="PHENOTYPE" checked="false" type="boolean" label="Input file contains phenotype information" truevalue="1" falsevalue="0" />
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244 <param argument="EXTRACOLS" value="0" type="integer" label="Number of additional columns of data before the genotype data start." />
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245
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246 <param argument="MARKERNAMES" checked="true" type="boolean" label="Data file contains row of marker names" truevalue="1" falsevalue="0" />
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247 <conditional name="recessivealleles_cond">
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248 <param argument="RECESSIVEALLELES" type="select" label="Data file contains dominant markers (eg AFLPs) and a row to indicate which alleles are recessive" >
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249 <option value="0" selected="True">No</option>
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250 <option value="1">Yes</option>
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251 </param>
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252 <when value="0">
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253 <param argument="NOTAMBIGUOUS" value="-999" type="hidden" label="Defines the code indicating that genotype data at a marker are unambiguous." help="For use with polyploids when RECESSIVEALLELES=1/True. Must not match MISSING or any allele value in the data." />
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254 </when>
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255 <when value="1">
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256 <param argument="NOTAMBIGUOUS" value="-999" type="integer" label="Defines the code indicating that genotype data at a marker are unambiguous." help="For use with polyploids when RECESSIVEALLELES=1/True. Must not match MISSING or any allele value in the data." />
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257 </when>
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258 </conditional>
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259 <param argument="MAPDISTANCES" checked="false" type="boolean" label="Data file contains row of map distances between loci" truevalue="1" falsevalue="0" />
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260
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261
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262 <!--Advanced data file options-->
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263
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264 <param argument="PHASED" checked="false" type="boolean" label="Data are in correct phase (relevant for linkage model only)" truevalue="1" falsevalue="0" />
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265 <param argument="PHASEINFO" checked="false" type="boolean" label="The data for each individual contains a line indicating phase (linkage model)" truevalue="1" falsevalue="0" />
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266 <param argument="MARKOVPHASE" checked="false" type="boolean" label="The phase info follows a Markov model." truevalue="1" falsevalue="0" />
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267 </section>
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268 <section name="extra" title="extraparams" expanded="False">
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269
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270 <param argument="NOADMIX" checked="false" type="boolean" label="Use no admixture model" help="(0/False=admixture model, 1/True=no-admix)" truevalue="1" falsevalue="0" />
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271 <param argument="LINKAGE" checked="false" type="boolean" label="Use the linkage model model" truevalue="1" falsevalue="0" />
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272 <conditional name="usepopinfo_cond">
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273 <param argument="USEPOPINFO" type="select" label="Use prior population information to pre-assign individuals to clusters">
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274 <option value="0" selected="True">No</option>
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275 <option value="1">Yes</option>
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276 </param>
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277 <when value="0">
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278 <param argument="POPFLAG" value="0" type="hidden" label="Input file contains a flag which says whether to use popinfo" help="[mainparams] when USEPOPINFO is 1/True" />
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279 <param argument="MIGRPRIOR" value="0.01" type="hidden" label="Prior prob that an individual is a migrant" help="(used only when USEPOPINFO==1/True). This should be small, eg 0.01 or 0.1." />
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280 </when>
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281 <when value="1">
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282 <param argument="POPFLAG" checked="false" type="boolean" label="Input file contains a flag which says whether to use popinfo" help="[mainparams] when USEPOPINFO is 1/True" truevalue="1" falsevalue="0" />
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283 <param argument="MIGRPRIOR" value="0.01" type="float" label="Prior prob that an individual is a migrant" help="(used only when USEPOPINFO==1/True). This should be small, eg 0.01 or 0.1." />
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284 </when>
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285 </conditional>
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286 <param argument="LOCPRIOR" checked="false" type="boolean" label="Use location information to improve weak data" truevalue="1" falsevalue="0" />
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287
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288 <param argument="FREQSCORR" checked="true" type="boolean" label="Allele frequencies are correlated among pops" truevalue="1" falsevalue="0" />
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289 <param argument="ONEFST" checked="false" type="boolean" label="Assume same value of Fst for all subpopulations" truevalue="1" falsevalue="0" />
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290
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291 <conditional name="inferalpha_cond">
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292 <param argument="INFERALPHA" type="select" label="Infer ALPHA (the admixture parameter)">
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293 <option value="1" selected="True">Yes</option>
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294 <option value="0">No</option>
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295 </param>
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296 <when value="1">
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297 <param argument="ALPHA" value="1.0" type="float" label="Dirichlet parameter for degree of admixture" help="this is the initial value if INFERALPHA is 1/True." />
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298 </when>
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299 <when value="0">
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300 <param argument="ALPHA" value="1.0" type="hidden" label="Dirichlet parameter for degree of admixture" help="this is the initial value if INFERALPHA is 1/True." />
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301 </when>
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302 </conditional>
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303 <param argument="POPALPHAS" checked="false" type="boolean" label="Individual alpha for each population" truevalue="1" falsevalue="0" />
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304
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305 <conditional name="inferlambda_cond">
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306 <param argument="INFERLAMBDA" type="select" label="Infer LAMBDA (the allele frequencies parameter)">
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307 <option value="0" selected="True">No</option>
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308 <option value="1">Yes</option>
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309 </param>
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310 <when value="0">
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311 <param argument="POPSPECIFICLAMBDA" value="0" type="hidden" label="Infer a separate lambda for each pop" help="(only if INFERLAMBDA=1/True)." />
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312 </when>
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313 <when value="1">
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314 <param argument="POPSPECIFICLAMBDA" checked="false" type="boolean" label="Infer a separate lambda for each pop" help="(only if INFERLAMBDA=1/True)." truevalue="1" falsevalue="0" />
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315 </when>
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316 </conditional>
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317 <param argument="LAMBDA" value="1.0" type="float" label="Dirichlet parameter for allele frequencies" />
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318
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319
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320 <!-- PRIORS -->
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321
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322 <param argument="FPRIORMEAN" value="0.01" type="float" label="The Prior (Gamma distribution) mean of Fst for pops." />
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323 <param argument="FPRIORSD" value="0.05" type="float" label="The Prior (Gamma distribution) Standard Deviation of Fst for pops." />
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324
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325 <conditional name="unifprioralpha_cond">
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326 <param argument="UNIFPRIORALPHA" type="select" label="Use a uniform prior for alpha; otherwise gamma prior">
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327 <option value="1" selected="True">Yes</option>
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328 <option value="0">No</option>
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329 </param>
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330 <when value="1">
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331 <param argument="ALPHAPRIORA" value="1.0" type="hidden" label="Alpha has a gamma prior with mean A*B, and variance A*B^2." help="(only if UNIFPRIORALPHA==0/False)" />
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332 <param argument="ALPHAPRIORB" value="2.0" type="hidden" label="Alpha has a gamma prior with mean A*B, and variance A*B^2." help="(only if UNIFPRIORALPHA==0/False)" />
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333 </when>
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334 <when value="0">
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335 <param argument="ALPHAPRIORA" value="1.0" type="float" label="Alpha has a gamma prior with mean A*B, and variance A*B^2." help="(only if UNIFPRIORALPHA==0/False)"/>
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336 <param argument="ALPHAPRIORB" value="2.0" type="float" label="Alpha has a gamma prior with mean A*B, and variance A*B^2." help="(only if UNIFPRIORALPHA==0/False)"/>
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337 </when>
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338 </conditional>
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339 <param argument="ALPHAMAX" value="10.0" type="float" label="Max value of alpha if uniform prior" />
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340
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341
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342 <param argument="LOG10RMIN" value="-4.0" type="float" label="Log10 of minimum allowed value of r under linkage model" />
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343 <param argument="LOG10RMAX" value="1.0" type="float" label="Log10 of maximum allowed value of r" />
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344 <param argument="LOG10RPROPSD" value="0.1" type="float" label="Standard deviation of log r in update" />
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345 <param argument="LOG10RSTART" value="-2.0" type="float" label="Initial value of log10 r" />
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346
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347
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348 <!-- USING PRIOR POPULATION INFO (USEPOPINFO) -->
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349
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350 <param argument="GENSBACK" value="2" type="integer" label="For use when inferring whether an individual is an immigrant, or has an immigrant an cestor in the past GENSBACK generations." help="eg, if GENSBACK==2, it tests for immigrant ancestry back to grandparents." />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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351 <param argument="PFROMPOPFLAGONLY" checked="false" type="boolean" label="Only use individuals with POPFLAG=1 to update P." help="This is to enable use of a reference set of individuals for clustering additional 'test' individuals." truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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352
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353 <!-- LOCPRIOR MODEL FOR USING LOCATION INFORMATION -->
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354
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355 <param argument="LOCISPOP" checked="true" type="boolean" label="Use POPDATA for location information" truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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356 <param argument="LOCPRIORINIT" value="1.0" type="float" label="Initial value for r, the location prior" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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357 <param argument="MAXLOCPRIOR" value="20.0" type="float" label="Max allowed value for r" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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358
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359 <!-- OUTPUT OPTIONS -->
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360
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361 <param argument="PRINTNET" checked="true" type="boolean" label="Print the 'net nucleotide distance' to screen during the run" truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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362 <param argument="PRINTLAMBDA" checked="true" type="boolean" label="Print current value(s) of lambda to screen" truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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363 <param argument="PRINTQSUM" checked="true" type="boolean" label="Print summary of current population membership to screen" truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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364
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365 <param argument="SITEBYSITE" checked="false" type="boolean" label="whether or not to print site by site results." help="(Linkage model only) This is a large file!" truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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366 <param argument="PRINTQHAT" checked="false" type="boolean" label="Q-hat printed to a separate file." help="Turn this on before using STRAT." truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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367 <param argument="UPDATEFREQ" value="100" type="integer" label="Frequency of printing update on the screen." help="Set automatically if this is 0/False." />
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368 <param argument="PRINTLIKES" checked="false" type="boolean" label="Print current likelihood to screen every rep" truevalue="1" falsevalue="0" />
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369 <param argument="INTERMEDSAVE" value="0" type="integer" label="Number of saves to file during run" />
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370
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371 <param argument="ECHODATA" checked="false" type="boolean" label="Print some of data file to screen to check that the data entry is correct." help="(NEXT 3 ARE FOR COLLECTING DISTRIBUTION OF Q:)" truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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372 <param argument="ANCESTDIST" checked="false" type="boolean" label="Collect data about the distribution of ancestry coefficients (Q) for each individual" truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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373 <param argument="NUMBOXES" value="1000" type="integer" label="The distribution of Q values is stored as a histogram with this number of boxes." />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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374 <param argument="ANCESTPINT" value="0.90" type="float" label="The size of the displayed probability interval on Q (values between 0.0--1.0)" />
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375
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376
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377
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378 <!-- MISCELLANEOUS -->
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379
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380 <param argument="COMPUTEPROB" checked="true" type="boolean" label="Estimate the probability of the Data under the model." help="This is used when choosing the best number of subpopulations." truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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381 <param argument="ADMBURNIN" value="500" type="integer" label="Initial period of burnin with admixture model" help="[only relevant for linkage model] see Documentation" />
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382 <param argument="ALPHAPROPSD" value="0.025" type="float" label="SD of proposal for updating alpha" />
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383 <param argument="STARTATPOPINFO" checked="false" type="boolean" label="Use given populations as the initial condition for population origins." help="(Need POPDATA==1). It is assumed that the PopData in the input file are between 1 and k where k is less or equal MAXPOPS." truevalue="1" falsevalue="0" />
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384 <conditional name="randomize_cond">
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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385 <param argument="RANDOMIZE" type="select" label="=use new random seed for each run">
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386 <option value="1" selected="True">Yes</option>
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387 <option value="0">No</option>
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388 </param>
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389 <when value="1">
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390 <param argument="SEED" value="2245" type="hidden" label="Seed value for random number generator" help="(must set RANDOMIZE=0)" />
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391 </when>
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392 <when value="0">
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393 <param argument="SEED" value="2245" type="integer" label="seed value for random number generator" help="(must set RANDOMIZE=0)" />
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394 </when>
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395 </conditional>
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396 <param argument="METROFREQ" value="10" type="integer" label="Frequency of using Metropolis step to update Q under admixture model" help="(ie use the metr. move every i steps). If this is set to 0, it is never used. (Proposal for each q^(i) sampled from prior. The goal is to improve mixing for small alpha.)" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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397 <param argument="REPORTHITRATE" checked="false" type="boolean" label="Report hit rate if using METROFREQ" truevalue="1" falsevalue="0" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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398 </section>
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399 </inputs>
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400 <outputs>
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401 <data name="out_mainparams" format="txt" label="run_K_${main.MAXPOPS}.mainparams" />
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402 <data name="out_extraparams" format="txt" label="run_K_${main.MAXPOPS}.extraparams" />
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403 <collection name="out" type="list" label="run_K_${main.MAXPOPS}.out">
1
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404 <discover_datasets pattern="__name__" format="txt" directory="out" />
0
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405 </collection>
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406 <collection name="log" type="list" label="run_K_${main.MAXPOPS}.log">
1
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407 <discover_datasets pattern="__name__" format="txt" directory="log" />
0
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408 </collection>
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409 </outputs>
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410 <tests>
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411 <test>
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412 <!-- https://web.stanford.edu/group/pritchardlab/structure_software/release_versions/v2.3.4/html/structure-data.html -->
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413 <param name="infile" value="testdata1" />
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414 <param name="nb_run" value="2" />
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415 <section name="main">
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416 <param name="NUMINDS" value="200" />
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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417 <param name="MAXPOPS" value="2" />
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418 <param name="LABEL" value="1" />
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419 <param name="POPDATA" value="1" />
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420 <param name="NUMLOCI" value="5" />
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421 <param name="LOCDATA" value="1" />
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422 <param name="PLOIDY" value="2" />
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423 <param name="MISSING" value="-999" />
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424 <param name="ONEROWPERIND" value="0" />
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425 <param name="MARKERNAMES" value="0" />
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426 </section>
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427 <section name="extra">
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428 <conditional name="randomize_cond">
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429 <param name="RANDOMIZE" value="0" />
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iuc
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diff changeset
430 </conditional>
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iuc
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diff changeset
431 </section>
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iuc
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diff changeset
432 <output_collection name="out" type="list">
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iuc
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diff changeset
433 <element name="run1_K_2.out" value="testdata1_f" lines_diff="6" />
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iuc
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diff changeset
434 <element name="run2_K_2.out" value="testdata1_f" lines_diff="6" />
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parents:
diff changeset
435 </output_collection>
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iuc
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diff changeset
436 <output_collection name="log" type="list">
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iuc
parents:
diff changeset
437 <element name="run1_K_2.log">
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iuc
parents:
diff changeset
438 <assert_contents>
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iuc
parents:
diff changeset
439 <has_line line="Final results printed to file outfile_f" />
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diff changeset
440 </assert_contents>
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diff changeset
441 </element>
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iuc
parents:
diff changeset
442 <element name="run2_K_2.log">
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iuc
parents:
diff changeset
443 <assert_contents>
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iuc
parents:
diff changeset
444 <has_line line="Final results printed to file outfile_f" />
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diff changeset
445 </assert_contents>
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parents:
diff changeset
446 </element>
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447 </output_collection>
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parents:
diff changeset
448 </test>
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449 </tests>
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parents:
diff changeset
450 <help><![CDATA[
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
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451 **Introduction**
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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parents:
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452
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diff changeset
453 The program structure_ implements a model-based clustering method for inferring population structure
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
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454 using genotype data consisting of unlinked markers. The method was introduced in a paper
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
455 by Pritchard, Stephens and Donnelly (2000a) and extended in sequels by Falush, Stephens and
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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456 Pritchard (2003a, 2007). Applications of our method include demonstrating the presence of population
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
457 structure, identifying distinct genetic populations, assigning individuals to populations, and
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
458 identifying migrants and admixed individuals.
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
459
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
460 Briefly, we assume a model in which there are K populations (where K may be unknown),
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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diff changeset
461 each of which is characterized by a set of allele frequencies at each locus. Individuals in the
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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diff changeset
462 sample are assigned (probabilistically) to populations, or jointly to two or more populations if their
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
463 genotypes indicate that they are admixed. It is assumed that within populations, the loci are at
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
464 Hardy-Weinberg equilibrium, and linkage equilibrium. Loosely speaking, individuals are assigned
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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diff changeset
465 to populations in such a way as to achieve this.
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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parents:
diff changeset
466
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
467 Our model does not assume a particular mutation process, and it can be applied to most of the
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
468 commonly used genetic markers including microsatellites, SNPs and RFLPs. The model assumes
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
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469 that markers are not in linkage disequilibrium (LD) within subpopulations, so we can’t handle
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
470 markers that are extremely close together. Starting with version 2.0, we can now deal with weakly
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
471 linked markers.
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
472
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
473 While the computational approaches implemented here are fairly powerful, some care is needed
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
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474 in running the program in order to ensure sensible answers. For example, it is not possible to
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
475 determine suitable run-lengths theoretically, and this requires some experimentation on the part of
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
476 the user. This document describes the use and interpretation of the software and supplements the
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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parents:
diff changeset
477 published papers, which provide more formal descriptions and evaluations of the methods.
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iuc
parents:
diff changeset
478
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iuc
parents:
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479 .. _structure: https://web.stanford.edu/group/pritchardlab/structure.html
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
480
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
481 **Documentation**
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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parents:
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482
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iuc
parents:
diff changeset
483 Please see the full Sructure documentation_
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iuc
parents:
diff changeset
484
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
485 .. _documentation: https://web.stanford.edu/group/pritchardlab/structure_software/release_versions/v2.3.4/structure_doc.pdf
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iuc
parents:
diff changeset
486
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iuc
parents:
diff changeset
487 **Upstream**
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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parents:
diff changeset
488
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iuc
parents:
diff changeset
489 Inputs can be produced from:
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490
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iuc
parents:
diff changeset
491 - Microsatellite analysis
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iuc
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492 - RADSeq analysis (eg: using populations_ from Stacks suite)
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parents:
diff changeset
493
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494 .. _populations: http://catchenlab.life.illinois.edu/stacks/manual/#export
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parents:
diff changeset
495
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parents:
diff changeset
496 **Input**
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parents:
diff changeset
497
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iuc
parents:
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498 ======= === ===== ===== ===== ===== =====
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iuc
parents:
diff changeset
499 loc_a loc_b loc_c loc_d loc_e
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iuc
parents:
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500 ======= === ===== ===== ===== ===== =====
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iuc
parents:
diff changeset
501 George 1 -9 145 66 0 92
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
502 George 1 -9 -9 64 0 94
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iuc
parents:
diff changeset
503 Paula 1 106 142 68 1 92
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iuc
parents:
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504 Paula 1 106 148 64 0 94
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iuc
parents:
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505 Matthew 2 110 145 -9 0 92
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
506 Matthew 2 110 148 66 1 -9
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
507 Bob 2 108 142 64 1 94
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iuc
parents:
diff changeset
508 Bob 2 -9 142 -9 0 94
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
509 Anja 1 112 142 -9 1 -9
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iuc
parents:
diff changeset
510 Anja 1 114 142 66 1 94
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iuc
parents:
diff changeset
511 Peter 1 -9 145 66 0 -9
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
512 Peter 1 110 145 -9 1 -9
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
513 Carsten 2 108 145 62 0 -9
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
514 Carsten 2 110 145 64 1 92
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
515 ======= === ===== ===== ===== ===== =====
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iuc
parents:
diff changeset
516
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iuc
parents:
diff changeset
517 You will find other sample data sets: here_
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
diff changeset
518
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iuc
parents:
diff changeset
519 .. _here: https://web.stanford.edu/group/pritchardlab/structure_software/release_versions/v2.3.4/html/structure-data.html
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iuc
parents:
diff changeset
520
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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521 **Downstream**
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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522
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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523 - Clumpp_
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
parents:
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524 - Distruct_
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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525 - Structure-harvester_
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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526
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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527 .. _Clumpp: https://rosenberglab.stanford.edu/clumpp.html
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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528 .. _Distruct: https://rosenberglab.stanford.edu/distruct.html
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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529 .. _Structure-harvester: http://taylor0.biology.ucla.edu/structureHarvester/
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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530
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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531 ]]></help>
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
iuc
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532 <citations>
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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533 <citation type="doi">10.1111/j.1471-8286.2007.01758.x</citation>
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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534 <citation type="doi">10.1111/j.1755-0998.2009.02591.x</citation>
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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535 </citations>
a1574aada200 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/structure commit b4d0a8f3dfee920840c77befdf626c52a5d617cb
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536 </tool>