Mercurial > repos > iuc > trinity_align_and_estimate_abundance
annotate align_and_estimate_abundance.xml @ 13:6d6b72d14170 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 3b8670ad0ce9dd90d6a24429bca131362e56d17d"
author | iuc |
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date | Fri, 01 Nov 2019 08:09:49 -0400 |
parents | aed859a021c0 |
children | 56162e446004 |
rev | line source |
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10
afd41a430a76
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 9dcdb5348f7439d0ce1e44ed23ad226beb4ae6d4
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1 <tool id="trinity_align_and_estimate_abundance" name="Align reads and estimate abundance" version="@WRAPPER_VERSION@"> |
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a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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2 <description>on a de novo assembly of RNA-Seq data</description> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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3 <macros> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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4 <import>macros.xml</import> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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5 </macros> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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6 <expand macro="requirements"> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 3b8670ad0ce9dd90d6a24429bca131362e56d17d"
iuc
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7 <requirement type="package" version="1.3.2">rsem</requirement> |
6d6b72d14170
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 3b8670ad0ce9dd90d6a24429bca131362e56d17d"
iuc
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8 <requirement type="package" version="0.46.0">kallisto</requirement> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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9 </expand> |
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10 <command detect_errors="aggressive"><![CDATA[ |
be3607a306af
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 7efdf3224552d113a01043ee5bf4517d770df933
iuc
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11 ln -f -s '$transcripts' input.fa && |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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12 |
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afd41a430a76
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 9dcdb5348f7439d0ce1e44ed23ad226beb4ae6d4
iuc
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13 #if $additional_params.gene_map.has_gene_map == "yes": |
afd41a430a76
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 9dcdb5348f7439d0ce1e44ed23ad226beb4ae6d4
iuc
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14 get_Trinity_gene_to_trans_map.pl input.fa > gene_to_trans.map && |
afd41a430a76
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 9dcdb5348f7439d0ce1e44ed23ad226beb4ae6d4
iuc
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15 #else: |
afd41a430a76
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 9dcdb5348f7439d0ce1e44ed23ad226beb4ae6d4
iuc
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16 ln -f -s '$additional_params.gene_map.gene_trans_map' gene_to_trans.map && |
afd41a430a76
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 9dcdb5348f7439d0ce1e44ed23ad226beb4ae6d4
iuc
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17 #end if |
afd41a430a76
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 9dcdb5348f7439d0ce1e44ed23ad226beb4ae6d4
iuc
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18 |
0
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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19 #if $inputs.paired_or_single == "paired": |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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20 #if $inputs.left_input.is_of_type('fasta'): |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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21 ln -s '$inputs.left_input' paired_left.fa && |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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22 ln -s '$inputs.right_input' paired_right.fa |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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23 #else: |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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24 ln -s '$inputs.left_input' paired_left.fq && |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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25 ln -s '$inputs.right_input' paired_right.fq |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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26 #end if |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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27 #else: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit b9a2194247d728ef4d5a3c5511aa6ea63e9b2bcb
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28 #if $inputs.input.is_of_type('fasta'): |
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a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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29 ln -s '$inputs.input' single.fa |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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30 #else: |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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31 ln -s '$inputs.input' single.fq |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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32 #end if |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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33 #end if |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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34 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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35 && |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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36 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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37 align_and_estimate_abundance.pl |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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38 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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39 --transcripts input.fa |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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40 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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41 --est_method $method.est_method |
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6d6b72d14170
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit 3b8670ad0ce9dd90d6a24429bca131362e56d17d"
iuc
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42 #if $method.est_method == "RSEM": |
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a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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43 --aln_method $method.aln_method |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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44 #else if $method.est_method == "salmon": |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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45 --salmon_idx_type $method.salmon_idx_type |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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46 #end if |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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47 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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48 #if $inputs.paired_or_single == "paired": |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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49 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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50 #if $inputs.left_input.is_of_type('fasta'): |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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51 --left paired_left.fa --right paired_right.fa --seqType fa |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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52 #else: |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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53 --left paired_left.fq --right paired_right.fq --seqType fq |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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54 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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55 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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56 #if $inputs.strand.is_strand_specific: |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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57 --SS_lib_type $inputs.strand.library_type |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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58 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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59 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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60 --max_ins_size $inputs.paired_fragment_length |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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61 |
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62 #else: |
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iuc
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63 #if $inputs.input.is_of_type('fasta'): |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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64 --single single.fa --seqType fa |
a21e229da9a1
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65 #else: |
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66 --single single.fq --seqType fq |
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67 #end if |
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68 |
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69 #if $inputs.strand.is_strand_specific: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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70 --SS_lib_type $inputs.strand.library_type |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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71 #end if |
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72 #end if |
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73 |
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74 ## Additional parameters. |
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75 --gene_trans_map gene_to_trans.map |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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76 |
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77 --prep_reference |
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78 |
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79 --output_dir output |
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80 |
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81 ## CPU |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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82 --thread_count \${GALAXY_SLOTS:-4} |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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83 ]]></command> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
84 <inputs> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
85 <param format="fasta" name="transcripts" argument="--transcripts" type="data" label="Transcripts" help="de novo assembly of RNA-Seq data"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
86 <conditional name="inputs"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
87 <param name="paired_or_single" type="select" label="Paired or Single-end data?"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
88 <option value="paired">Paired</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
89 <option value="single">Single</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
90 </param> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
91 <when value="paired"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
92 <param format="fasta,fastqsanger" name="left_input" argument="--left" type="data" label="Left/Forward strand reads" help=""/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
93 <param format="fasta,fastqsanger" name="right_input" argument="--right" type="data" label="Right/Reverse strand reads" help=""/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
94 <conditional name="strand"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
95 <param name="is_strand_specific" type="boolean" checked="false" label="Strand specific data"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
96 <when value="false"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
97 </when> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
98 <when value="true"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
99 <param name="library_type" argument="--SS_lib_type" type="select" label="Strand-specific Library Type"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
100 <option value="FR">Forward-Reverse</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
101 <option value="RF">Reverse-Forward</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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102 </param> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
103 </when> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
104 </conditional> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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105 <param name="paired_fragment_length" argument="--max_ins_size" type="integer" value="800" min="1" label="Maximum insert size" help="bowtie -X parameter"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
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106 </when> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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107 <when value="single"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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changeset
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108 <param format="fasta,fastqsanger" argument="--single" name="input" type="data" label="Single-end reads" help=""/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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109 <conditional name="strand"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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110 <param name="is_strand_specific" type="boolean" checked="false" label="Strand specific data"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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111 <when value="false"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
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112 </when> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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113 <when value="true"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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114 <param name="library_type" argument="--SS_lib_type" type="select" label="Strand-specific Library Type"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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115 <option value="F">F</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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changeset
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116 <option value="R">R</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
117 </param> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
118 </when> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
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119 </conditional> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
120 </when> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
121 </conditional> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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122 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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123 <conditional name="method"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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124 <param type="select" name="est_method" argument="--est_method" label="Abundance estimation method"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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125 <option value="RSEM">RSEM</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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126 <option value="salmon">Salmon</option> |
6
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
iuc
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5
diff
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127 <option value="kallisto">Kallisto</option> |
0
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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128 </param> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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129 <when value="RSEM"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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130 <param type="select" name="aln_method" argument="--aln_method" label="Alignment method"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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changeset
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131 <option value="bowtie">Bowtie</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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132 <option value="bowtie2">Bowtie2</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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133 </param> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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134 </when> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
135 <when value="salmon"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
136 <param type="select" name="salmon_idx_type" argument="--salmon_idx_type" label="Index type"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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137 <option value="quasi">Quasi</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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138 <option value="fmd">FMD</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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139 </param> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
140 </when> |
6
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
iuc
parents:
5
diff
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141 <when value="kallisto"> |
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
iuc
parents:
5
diff
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142 </when> |
0
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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143 </conditional> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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144 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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145 <section name="additional_params" title="Additional Options" expanded="False"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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changeset
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146 <conditional name="gene_map"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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changeset
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147 <param name="has_gene_map" type="select" label="Trinity assembly?" help="If the transcripts were not assembled by trinity, additional information is needed"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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148 <option value="yes">Yes</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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149 <option value="no">No</option> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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150 </param> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
diff
changeset
|
151 <when value="yes"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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152 </when> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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153 <when value="no"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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154 <param format="tabular" name="gene_trans_map" argument="--gene_trans_map" type="data" label="Gene to transcript correspondence ('gene(tab)transcript' lines)" /> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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155 </when> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
156 </conditional> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
157 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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158 </section> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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159 </inputs> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
160 <outputs> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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|
161 <data format="tabular" name="isoforms_counts_rsem" label="${tool.name} on ${on_string}: isoforms counts" from_work_dir="output/RSEM.isoforms.results"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
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162 <filter>method['est_method'] == "RSEM"</filter> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
parents:
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changeset
|
163 </data> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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164 <data format="tabular" name="genes_counts_rsem" label="${tool.name} on ${on_string}: genes counts" from_work_dir="output/RSEM.genes.results"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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changeset
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165 <filter>method['est_method'] == "RSEM"</filter> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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166 </data> |
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iuc
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167 |
a21e229da9a1
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iuc
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168 |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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169 <data format="tabular" name="isoforms_counts_salmon" label="${tool.name} on ${on_string}: isoforms counts" from_work_dir="output/quant.sf"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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170 <filter>method['est_method'] == "salmon"</filter> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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171 </data> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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172 <data format="tabular" name="genes_counts_salmon" label="${tool.name} on ${on_string}: genes counts" from_work_dir="output/quant.sf.genes"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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173 <filter>method['est_method'] == "salmon"</filter> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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174 </data> |
6
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
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175 |
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
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176 <data format="tabular" name="isoforms_counts_kallisto" label="${tool.name} on ${on_string}: isoforms counts" from_work_dir="output/abundance.tsv"> |
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
iuc
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177 <filter>method['est_method'] == "kallisto"</filter> |
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
iuc
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178 </data> |
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
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179 <data format="tabular" name="genes_counts_kallisto" label="${tool.name} on ${on_string}: genes counts" from_work_dir="output/abundance.tsv.genes"> |
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
iuc
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180 <filter>method['est_method'] == "kallisto"</filter> |
04bd98a9c751
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
iuc
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181 </data> |
0
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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182 </outputs> |
a21e229da9a1
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iuc
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183 <tests> |
a21e229da9a1
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iuc
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184 <test> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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185 <param name="paired_or_single" value="paired"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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186 <param name="left_input" value="reads.left.fq"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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187 <param name="right_input" value="reads.right.fq"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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188 <param name="transcripts" value="raw/Trinity.fasta"/> |
10
afd41a430a76
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iuc
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189 <param name="gene_to_trans" value="raw/Trinity.map" /> |
0
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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190 <param name="library_type" value="RF"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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191 <param name="est_method" value="RSEM"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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192 <param name="aln_method" value="bowtie"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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193 <param name="has_gene_map" value="yes"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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194 <output name="isoforms_counts_rsem"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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195 <assert_contents> |
a21e229da9a1
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iuc
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196 <has_line_matching expression="TRINITY_DN0_c0_g1_i1	.*" /> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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197 <has_n_columns n="8" /> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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198 </assert_contents> |
a21e229da9a1
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iuc
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199 </output> |
a21e229da9a1
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iuc
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200 <output name="genes_counts_rsem"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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201 <assert_contents> |
a21e229da9a1
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iuc
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202 <has_line_matching expression="TRINITY_DN0_c0_g1	.*" /> |
a21e229da9a1
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iuc
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203 <has_n_columns n="7" /> |
a21e229da9a1
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iuc
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204 </assert_contents> |
a21e229da9a1
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iuc
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205 </output> |
a21e229da9a1
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iuc
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206 </test> |
a21e229da9a1
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iuc
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207 <test> |
a21e229da9a1
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208 <param name="paired_or_single" value="paired"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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209 <param name="left_input" value="reads.left.fq"/> |
a21e229da9a1
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iuc
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210 <param name="right_input" value="reads.right.fq"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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211 <param name="transcripts" value="raw/Trinity.fasta"/> |
10
afd41a430a76
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iuc
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212 <param name="gene_to_trans" value="raw/Trinity.map" /> |
0
a21e229da9a1
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iuc
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213 <param name="library_type" value="RF"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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214 <param name="est_method" value="RSEM"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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215 <param name="aln_method" value="bowtie2"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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216 <param name="has_gene_map" value="yes"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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217 <output name="isoforms_counts_rsem"> |
a21e229da9a1
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218 <assert_contents> |
a21e229da9a1
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219 <has_line_matching expression="TRINITY_DN0_c0_g1_i1	.*" /> |
a21e229da9a1
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220 <has_n_columns n="8" /> |
a21e229da9a1
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iuc
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221 </assert_contents> |
a21e229da9a1
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iuc
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222 </output> |
a21e229da9a1
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iuc
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223 <output name="genes_counts_rsem"> |
a21e229da9a1
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iuc
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224 <assert_contents> |
a21e229da9a1
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iuc
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225 <has_line_matching expression="TRINITY_DN0_c0_g1	.*" /> |
a21e229da9a1
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226 <has_n_columns n="7" /> |
a21e229da9a1
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227 </assert_contents> |
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228 </output> |
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229 </test> |
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230 <test> |
a21e229da9a1
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231 <param name="paired_or_single" value="paired"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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232 <param name="left_input" value="reads.left.fq"/> |
a21e229da9a1
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iuc
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233 <param name="right_input" value="reads.right.fq"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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234 <param name="transcripts" value="raw/Trinity.fasta"/> |
10
afd41a430a76
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iuc
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235 <param name="gene_to_trans" value="raw/Trinity.map" /> |
0
a21e229da9a1
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iuc
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236 <param name="library_type" value="RF"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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237 <param name="est_method" value="salmon"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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238 <param name="aln_method" value="bowtie"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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239 <param name="has_gene_map" value="yes"/> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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240 <output name="isoforms_counts_salmon"> |
a21e229da9a1
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iuc
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241 <assert_contents> |
a21e229da9a1
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iuc
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242 <has_line_matching expression="TRINITY_DN2_c3_g1_i1	.*" /> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
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243 <has_n_columns n="5" /> |
a21e229da9a1
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iuc
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244 </assert_contents> |
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245 </output> |
a21e229da9a1
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iuc
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246 <output name="genes_counts_salmon"> |
a21e229da9a1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit e23a8ad798830209db722d5496d19ec7a5e06214
iuc
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247 <assert_contents> |
a21e229da9a1
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248 <has_line_matching expression="TRINITY_DN3_c0_g1.*" /> |
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249 <has_n_columns n="5" /> |
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250 </assert_contents> |
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251 </output> |
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252 </test> |
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253 <test> |
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254 <param name="paired_or_single" value="paired"/> |
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255 <param name="left_input" value="reads.left.fq"/> |
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256 <param name="right_input" value="reads.right.fq"/> |
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257 <param name="transcripts" value="raw/Trinity.fasta"/> |
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258 <param name="gene_to_trans" value="raw/Trinity.map" /> |
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259 <param name="library_type" value="RF"/> |
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260 <param name="est_method" value="kallisto"/> |
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261 <param name="has_gene_map" value="yes"/> |
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262 <output name="isoforms_counts_kallisto"> |
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263 <assert_contents> |
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264 <has_line_matching expression="TRINITY_DN1_c0_g1_i1	.*" /> |
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265 <has_n_columns n="5" /> |
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266 </assert_contents> |
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267 </output> |
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268 <output name="genes_counts_kallisto"> |
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269 <assert_contents> |
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270 <has_line_matching expression="TRINITY_DN1_c0_g1	.*" /> |
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271 <has_n_columns n="5" /> |
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272 </assert_contents> |
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273 </output> |
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274 </test> |
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275 </tests> |
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276 <help> |
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277 <![CDATA[ |
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278 Trinity_ assembles transcript sequences from Illumina RNA-Seq data. |
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279 This tool estimates the abundance of isoforms and genes of a transcriptome assembled with Trinity, using FastQ of a specific sample. |
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280 |
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281 **Inputs** |
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282 |
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283 It takes as input a transcriptome assembled with Trinity and the reads from a RNASeq sample. |
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284 You have to choose between several counting methods. |
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285 |
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286 If you dont align on a Trinity assembly, you need to provide a file of the following (tabular) format to map gene ids to transcript ids: |
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287 |
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288 =========== ================ |
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289 gene1 transcript1 |
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290 ----------- ---------------- |
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291 gene2 transcript2 |
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292 =========== ================ |
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293 |
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294 **Output** |
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295 |
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296 This tool will produce 2 tabular files, with counts for isoforms and genes respectively. More details on this page: |
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297 |
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298 .. _Trinity manual: https://github.com/trinityrnaseq/trinityrnaseq/wiki/Trinity-Transcript-Quantification |
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299 |
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300 |
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301 .. _Trinity: http://trinityrnaseq.github.io |
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302 ]]> |
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303 </help> |
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304 |
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305 <expand macro="citation" /> |
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306 </tool> |