Mercurial > repos > iuc > unicycler

changeset 6:0a3a602cd1e3 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/unicycler commit 7272906b45bad9ad1eb9bd01ee4e8936fc4c20a5
author iuc
date Sat, 09 Feb 2019 17:02:48 -0500
parents 23300b42ca18
children 88c240872a65
files unicycler.xml
diffstat 1 files changed, 33 insertions(+), 17 deletions(-) [+]
line wrap: on
line diff
--- a/unicycler.xml	Wed Sep 19 17:07:02 2018 -0400
+++ b/unicycler.xml	Sat Feb 09 17:02:48 2019 -0500
@@ -1,6 +1,6 @@
 <tool id="unicycler" name="Create assemblies with Unicycler" version="@VERSION@.0">
     <macros>
-        <token name="@VERSION@">0.4.6</token>
+        <token name="@VERSION@">0.4.7</token>
     </macros>
     <requirements>
         <requirement type="package" version="@VERSION@">unicycler</requirement>
@@ -125,14 +125,17 @@
                 <option value="none">None</option>
             </param>
             <when value="paired">
-                <param name="fastq_input1" argument="-1" type="data" format="fastqsanger,fastqsanger.gz" label="Select first set of reads" help="Specify dataset with forward reads"/>
-                <param name="fastq_input2" argument="-2" type="data" format="fastqsanger,fastqsanger.gz" label="Select second set of reads" help="Specify dataset with reverse reads"/>
+                <param name="fastq_input1" argument="-1" type="data" format="fastqsanger,fastqsanger.gz"
+                    label="Select first set of reads" help="Specify dataset with forward reads"/>
+                <param name="fastq_input2" argument="-2" type="data" format="fastqsanger,fastqsanger.gz"
+                    label="Select second set of reads" help="Specify dataset with reverse reads"/>
             </when>
             <when value="paired_collection">
                 <param name="fastq_input1" format="fastqsanger,fastqsanger.gz" type="data_collection" collection_type="paired" label="Select a paired collection" />
             </when>
             <when value="single">
-                <param name="fastq_input1" argument="-s" type="data" format="fastqsanger,fastqsanger.gz" label="Select unpaired reads" help="Specify dataset with unpaired reads"/>
+                <param name="fastq_input1" argument="-s" type="data" format="fastqsanger,fastqsanger.gz"
+                    label="Select unpaired reads" help="Specify dataset with unpaired reads"/>
             </when>
             <when value="none">
             </when>
@@ -146,34 +149,47 @@
         <param argument="--min_fasta_length" type="integer" value="100" label="Exclude contigs from the FASTA file which are shorter than this length (bp)"/>
         <param argument="--linear_seqs" type="integer" value="0" label="The expected number of linear (i.e. non-circular) sequences in the assembly"/>
         <param argument="--min_anchor_seg_len" type="integer" min="0" optional="true" label="Unicycler will not use segments shorter than this as scaffolding anchors"/>
-        <section name="spades" expanded="False" title="SPAdes options" help="Unicycler uses SPAdes to construct assembly graphs. You can modify some of the SPAdes settings here. Use this ONLY if you know what you are doing!">
-            <param argument="--no_correct" type="boolean" checked="false" truevalue="--no_correct" falsevalue="" label="Skip SPAdes error correction step" help="This option turns off SPAdes error correction. Generally it is highly recommended to use correction."/>
-            <param argument="--min_kmer_frac" type="float" min="0" max="1" value="0.2" label="Lowest k-mer size for SPAdes assembly, expressed as a fraction of the read length"/>
-            <param argument="--max_kmer_frac" type="float" min="0" max="1" value="0.95" label="Highest k-mer size for SPAdes assembly, expressed as a fraction of the read length"/>
+        <section name="spades" expanded="False" title="SPAdes options"
+            help="Unicycler uses SPAdes to construct assembly graphs. You can modify some of the SPAdes settings here. Use this ONLY if you know what you are doing!">
+            <param argument="--no_correct" type="boolean" checked="false" truevalue="--no_correct" falsevalue=""
+                label="Skip SPAdes error correction step" help="This option turns off SPAdes error correction. Generally it is highly recommended to use correction."/>
+            <param argument="--min_kmer_frac" type="float" min="0" max="1" value="0.2"
+                label="Lowest k-mer size for SPAdes assembly, expressed as a fraction of the read length"/>
+            <param argument="--max_kmer_frac" type="float" min="0" max="1" value="0.95"
+                label="Highest k-mer size for SPAdes assembly, expressed as a fraction of the read length"/>
             <param argument="--kmers" type="text" value="" optional="true" label="Exact k-mers to use for SPAdes assembly, comma-separated">
                 <validator type="regex" message="Kmers must be comma-separated odd integers (no repitition) without space in the range of 11 to 127 (inclusive)">^(\d*[13579],)*(\d*[13579])$</validator>
             </param>
             <param argument="--kmer_count" type="integer" min="0" value="10" label="Number of k-mer steps to use in SPAdes assembly"/>
-            <param argument="--depth_filter" type="float" min="0" max="1" value="0.25" label="Filter out contigs lower than this fraction of the chromosomal depth" help="It is done if does not result in graph dead ends"/>
+            <param argument="--depth_filter" type="float" min="0" max="1" value="0.25"
+                label="Filter out contigs lower than this fraction of the chromosomal depth" help="It is done if does not result in graph dead ends"/>
         </section>
-        <section name="rotation" expanded="false" title="Rotation options" help="These options control the rotation of completed circular sequence near the end of the Unicycler pipeline. Use this ONLY if you know what you are doing!">
-            <param argument="--no_rotate" type="boolean" checked="false" truevalue="--no_rotate" falsevalue="" label="Do not rotate completed replicons to start at a standard gene." help="Unicycler uses TBLASTN to search for dnaA or repA alleles in each completed replicon. If one is found, the sequence is rotated and/or flipped so that it begins with that gene encoded on the forward strand. This provides consistently oriented assemblies and reduces the risk that a gene will be split across the start and end of the sequence."/>
+        <section name="rotation" expanded="false" title="Rotation options"
+            help="These options control the rotation of completed circular sequence near the end of the Unicycler pipeline. Use this ONLY if you know what you are doing!">
+            <param argument="--no_rotate" type="boolean" checked="false" truevalue="--no_rotate" falsevalue=""
+                label="Do not rotate completed replicons to start at a standard gene." help="Unicycler uses TBLASTN to search for dnaA or repA alleles in each completed replicon. If one is found, the sequence is rotated and/or flipped so that it begins with that gene encoded on the forward strand. This provides consistently oriented assemblies and reduces the risk that a gene will be split across the start and end of the sequence."/>
             <param argument="--start_genes" optional="true" type="data" format="fasta" label="FASTA file of genes for start point of rotated replicons" />
             <param argument="--start_gene_id" type="float" min="0" max="100" value="90" label="The minimum required BLAST percent identity for a start gene search"/>
             <param argument="--start_gene_cov" type="float" min="0" max="100" value="95" label="The minimum required BLAST percent coverage for a start gene search"/>
         </section>
         <section name="pilon" title="Pilon options" expanded="false">
-            <param argument="--no_pilon" type="boolean" checked="false" truevalue="--no_pilon" falsevalue="" label="Do not use Pilon to polish the final assembly." help="Unicycler uses Pilon tool for polishing final assembly."/>
+            <param argument="--no_pilon" type="boolean" checked="false" truevalue="--no_pilon" falsevalue=""
+                label="Do not use Pilon to polish the final assembly." help="Unicycler uses Pilon tool for polishing final assembly."/>
             <param argument="--min_polish_size" type="integer" min="0" value="1000" label="Contigs shorter than this value (bp) will not be polished using Pilon"/>
         </section>
-        <section name="graph_clean" expanded="false" title="Graph cleaning options" help="These options control the removal of small leftover sequences after bridging is complete.">
-            <param argument="--min_component_size" type="integer" min="0" value="1000" label="Unbridged graph components smaller than this size will be removed from the final graph" />
-            <param argument="--min_dead_end_size" type="integer" min="0" value="1000" label="Graph dead ends smaller than this size will be removed from the final graph"/>
+        <section name="graph_clean" expanded="false" title="Graph cleaning options"
+            help="These options control the removal of small leftover sequences after bridging is complete.">
+            <param argument="--min_component_size" type="integer" min="0" value="1000"
+                label="Unbridged graph components smaller than this size will be removed from the final graph" />
+            <param argument="--min_dead_end_size" type="integer" min="0" value="1000"
+                label="Graph dead ends smaller than this size will be removed from the final graph"/>
         </section>
         <section name="lr_align" expanded="false" title="Long read alignment parameters" help="These options control the alignment of long reads to the assembly graph.">
-            <param argument="--contamination" optional="true" type="data" format="fasta" label="FASTA file of known contamination in long reads, e.g. lambda, phiXm or puc18 spike-ins." />
+            <param argument="--contamination" optional="true" type="data" format="fasta"
+                label="FASTA file of known contamination in long reads, e.g. lambda, phiXm or puc18 spike-ins." />
             <param argument="--scores" type="text" value="3,-6,-5,-2" label="Comma-delimited string of alignment scores: match, mismatch, gap open, gap extend"/>
-            <param argument="--low_score" optional="true" type="integer" value="" label="Score threshold - alignments below this are considered poor" help="default = set automatically"/>
+            <param argument="--low_score" optional="true" type="integer" value=""
+                label="Score threshold - alignments below this are considered poor" help="default = set automatically"/>
         </section>
     </inputs>
     <outputs>