Mercurial > repos > iuc > vsearch
annotate search.xml @ 2:f29e21388219 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
author | iuc |
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date | Thu, 17 Dec 2015 12:53:39 -0500 |
parents | fae6527990af |
children | 4258854759ba |
rev | line source |
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2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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1 <tool id="vsearch_search" name="VSearch search" version="@VERSION@.1"> |
0 | 2 <description></description> |
3 <macros> | |
4 <import>vsearch_macros.xml</import> | |
5 </macros> | |
6 <expand macro="requirements" /> | |
7 <expand macro="stdio" /> | |
8 <expand macro="version_command" /> | |
9 <command> | |
10 <![CDATA[ | |
11 vsearch | |
12 | |
13 @GENERAL@ | |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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14 --db "$dbfile" |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
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changeset
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15 #if $dbmask: |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
0
diff
changeset
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16 --dbmask "$dbmask" |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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17 #end if |
0 | 18 |
19 ##--fulldp full dynamic programming alignment (always on) | |
20 ##--gapext STRING penalties for gap extension (2I/1E) | |
21 ##--gapopen STRING penalties for gap opening (20I/2E) | |
22 $hardmask | |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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23 --id "$id" |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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24 --iddef "$iddef" |
0 | 25 |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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26 #if str( $qmask ) != 'no': |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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27 --qmask "$qmask" |
0 | 28 #end if |
29 | |
30 ## --rowlen INT width of alignment lines in alnout output (64) | |
31 | |
32 $self_param | |
33 $selfid_param | |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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34 $sizeout |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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35 --strand "$strand" |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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diff
changeset
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36 --usearch_global "$queryfile" |
0 | 37 |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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38 #if '--alnout' in str($outputs).split( "," ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
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diff
changeset
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39 --alnout "$alnout" |
0 | 40 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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diff
changeset
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41 #if '--blast6out' in str($outputs).split( "," ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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diff
changeset
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42 --blast6out "$blast6out" |
0 | 43 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
0
diff
changeset
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44 #if '--dbmatched' in str($outputs).split( "," ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
0
diff
changeset
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45 --dbmatched "$dbmatched" |
0 | 46 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
0
diff
changeset
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47 #if '--dbnotmatched' in str($outputs).split( "," ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
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diff
changeset
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48 --dbnotmatched "$dbnotmatched" |
0 | 49 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
0
diff
changeset
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50 #if '--fastapairs' in str($outputs).split( "," ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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51 --fastapairs "$fastapairs" |
0 | 52 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
0
diff
changeset
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53 #if '--notmatched' in str($outputs).split( "," ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
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diff
changeset
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54 --notmatched "$notmatched" |
0 | 55 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
parents:
0
diff
changeset
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56 #if '--matched' in str($outputs).split( "," ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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57 --matched "$matched" |
0 | 58 #end if |
59 | |
60 #if $adv_opts.adv_opts_selector == "advanced": | |
61 $adv_opts.top_hits_only | |
62 $adv_opts.rightjust | |
63 $adv_opts.leftjust | |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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64 --target_cov "$adv_opts.target_cov" |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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65 --query_cov "$adv_opts.query_cov" |
0 | 66 |
67 | |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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68 #if str( $adv_opts.maxid ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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69 --maxid "$adv_opts.maxid" |
0 | 70 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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changeset
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71 #if str( $adv_opts.maxqt ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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72 --maxqt "$adv_opts.maxqsize" |
0 | 73 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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74 #if str( $adv_opts.maxsizeratio ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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75 --maxsizeratio "$adv_opts.maxsizeratio" |
0 | 76 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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77 #if str( $adv_opts.maxsl ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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78 --maxsl "$adv_opts.maxsl" |
0 | 79 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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80 #if str( $adv_opts.mid ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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81 --mid "$adv_opts.mid" |
0 | 82 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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83 #if str( $adv_opts.minqt ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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84 --minqt "$adv_opts.minqt" |
0 | 85 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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86 #if str( $adv_opts.minsizeratio ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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87 --minsizeratio "$adv_opts.minseqlength" |
0 | 88 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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89 #if str( $adv_opts.minsl ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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90 --minsl "$adv_opts.minsl" |
0 | 91 #end if |
92 | |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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93 #if str( $adv_opts.mintsize ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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94 --mintsize "$adv_opts.mintsize" |
0 | 95 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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96 #if str( $adv_opts.mismatch ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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97 --mismatch "$adv_opts.mismatch" |
0 | 98 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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99 #if str( $adv_opts.maxqsize ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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100 --maxqsize "$adv_opts.maxqsize" |
0 | 101 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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102 #if str( $adv_opts.mincols ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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103 --mincols "$adv_opts.mincols" |
0 | 104 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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105 #if str( $adv_opts.maxsubs ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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106 --maxsubs "$adv_opts.maxsubs" |
0 | 107 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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108 #if str( $adv_opts.maxrejects ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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109 --maxrejects "$adv_opts.maxrejects" |
0 | 110 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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111 #if str( $adv_opts.maxaccepts ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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112 --maxaccepts "$adv_opts.maxaccepts" |
0 | 113 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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114 #if str( $adv_opts.maxdiffs ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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115 --maxdiffs "$adv_opts.maxdiffs" |
0 | 116 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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117 #if str( $adv_opts.maxgaps ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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118 --maxgaps "$adv_opts.maxgaps" |
0 | 119 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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120 #if str( $adv_opts.maxhits ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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121 --maxhits "$adv_opts.maxhits" |
0 | 122 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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123 #if str( $adv_opts.match ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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124 --match "$adv_opts.match" |
0 | 125 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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126 #if str( $adv_opts.idprefix ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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127 --idprefix "$adv_opts.idprefix" |
0 | 128 #end if |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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129 #if str( $adv_opts.idsuffix ): |
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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130 --idsuffix str( $adv_opts.idsuffix ) |
0 | 131 #end if |
132 | |
133 #if $adv_opts.uclust_output.uclust_output_select == 'yes': | |
2
f29e21388219
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsearch commit 64c1c4c664c6a131d897f574dc849f5668fa97d2
iuc
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134 --uc "$uc" |
0 | 135 $adv_opts.uclust_output.uc_allhits |
136 #end if | |
137 | |
138 #if $adv_opts.userfields_output.userfields_output_select == 'yes': | |
139 --userfields '#echo '+'.join( str($adv_opts.userfields_output.userfields).split(',') )#' | |
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140 --userout "$userout" |
0 | 141 #end if |
142 | |
143 ##--weak_id REAL include aligned hits with >= id; continue search | |
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144 --wordlength "$adv_opts.wordlength" |
0 | 145 |
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146 $adv_opts.output_no_hits |
0 | 147 |
148 #end if | |
149 | |
150 | |
151 ]]> | |
152 </command> | |
153 <inputs> | |
154 <param name="queryfile" type="data" format="fasta" label="Select your query FASTA file" help="(--usearch_global)" /> | |
155 <param name="dbfile" type="data" format="fasta" label="Select your database FASTA file" help="(--db)" /> | |
156 | |
157 <expand macro="qmask" /> | |
158 <expand macro="sizeout" /> | |
159 <expand macro="strand" /> | |
160 <expand macro="self_and_selfid" /> | |
161 <expand macro="id_and_iddef" /> | |
162 <expand macro="hardmask" /> | |
163 | |
164 <param name="dbmask" type="select" optional="True" label="Mask database sequences" help="(--dbmask)"> | |
165 <option value="none" selected="True">No masking</option> | |
166 <option value="dust" selected="True">dust</option> | |
167 <option value="soft">soft</option> | |
168 </param> | |
169 | |
170 <expand macro="general_output"> | |
171 <option value="--dbmatched">FASTA file for matching database sequences</option> | |
172 <option value="--dbnotmatched">FASTA file for non-matching database sequences</option> | |
173 <option value="--notmatched">Write non-matching query sequences to separate file</option> | |
174 <option value="--matched">Write matching query sequences to separate file</option> | |
175 </expand> | |
176 | |
177 <conditional name="adv_opts"> | |
178 <param name="adv_opts_selector" type="select" label="Advanced Options"> | |
179 <option value="basic" selected="True">Hide Advanced Options</option> | |
180 <option value="advanced">Show Advanced Options</option> | |
181 </param> | |
182 <when value="basic" /> | |
183 <when value="advanced"> | |
184 | |
185 <param name="top_hits_only" type="boolean" truevalue="--top_hits_only" falsevalue="" checked="False" | |
186 label="Output only hits with identity equal to the best" help="(--top_hits_only)"/> | |
187 <param name="rightjust" type="boolean" truevalue="--rightjust" falsevalue="" checked="False" | |
188 label="Reject if terminal gaps at alignment right end" help="(--rightjust)"/> | |
189 <param name="leftjust" type="boolean" truevalue="--leftjust" falsevalue="" checked="False" | |
190 label="Reject if terminal gaps at alignment left end" help="(--leftjust)"/> | |
191 | |
192 <param name="query_cov" type="float" value="" optional="True" label="Reject if fraction of query seq. aligned lower than this value" | |
193 help="(--query_cov)"/> | |
194 <param name="target_cov" type="float" value="" optional="True" label="Reject if fraction of target seq. aligned lower than this value" | |
195 help="(--target_cov)"/> | |
196 <param name="maxid" type="float" value="" optional="True" label="Reject if identity higher than this value" | |
197 help="(--maxid)"/> | |
198 <param name="maxqt" type="float" value="" optional="True" label="Reject if query/target length ratio higher than this value" | |
199 help="(--maxqt)"/> | |
200 <param name="maxsizeratio" type="float" value="" optional="True" label="Reject if query/target abundance ratio higher than this value" | |
201 help="(--maxsizeratio)"/> | |
202 <param name="maxsl" type="float" value="" optional="True" label="Reject if shorter/longer length ratio higher than this value" | |
203 help="(--maxsl)"/> | |
204 <param name="mid" type="float" value="" optional="True" label="Reject if percent identity lower than this value, ignoring gaps" | |
205 help="(--mid)"/> | |
206 <param name="minqt" type="float" value="" optional="True" label="Reject if query/target length ratio lower than this value" | |
207 help="(--minqt)"/> | |
208 <param name="minsl" type="float" value="" optional="True" label="Reject if shorter/longer length ratio lower than this value" | |
209 help="(--minsl)"/> | |
210 <param name="minsizeratio" type="float" value="" optional="True" label="Reject if query/target abundance ratio lower than this value" | |
211 help="(--minsizeratio)"/> | |
212 | |
213 <param name="maxqsize" type="integer" value="" optional="True" label="Reject if query abundance larger than this value" | |
214 help="(--maxqsize)"/> | |
215 <param name="mincols" type="integer" value="" optional="True" label="Reject if alignment length shorter than this value" | |
216 help="(--mincols)"/> | |
217 <param name="maxsubs" type="integer" value="" optional="True" label="Reject if more substitutions than this value" | |
218 help="(--maxsubs)"/> | |
219 <expand macro="maxrejects" /> | |
220 <expand macro="maxaccepts" /> | |
221 <param name="maxdiffs" type="integer" value="" optional="True" label="Reject if more substitutions or indels than this value" | |
222 help="(--maxdiffs)"/> | |
223 <param name="maxgaps" type="integer" value="" optional="True" label="Reject if more indels than this value" | |
224 help="(--maxgaps)"/> | |
225 <param name="maxhits" type="integer" value="" optional="True" label="Maximum number of hits to show" | |
226 help="(--maxhits)"/> | |
227 <param name="match" type="integer" value="2" label="Score for match" | |
228 help="(--match)"/> | |
229 <param name="idprefix" type="integer" value="" optional="True" label="Reject if first n nucleotides do not match" | |
230 help="(--idprefix)"/> | |
231 <param name="idsuffix" type="integer" value="" optional="True" label="Reject if last n nucleotides do not match" | |
232 help="(--idsuffix)"/> | |
233 <param name="mintsize" type="integer" value="" optional="True" label="Reject if target abundance lower" | |
234 help="(--mintsize)"/> | |
235 <param name="mismatch" type="integer" value="-4" label="Score for mismatch" | |
236 help="(--mismatch)"/> | |
237 | |
238 <conditional name="uclust_output"> | |
239 <param name="uclust_output_select" type="select" label="UCLUST-like output" help="(--uc)"> | |
240 <option value="yes">Yes</option> | |
241 <option value="no" selected="True">No</option> | |
242 </param> | |
243 <when value="yes"> | |
244 <param name="uc_allhits" type="boolean" truevalue="--uc_allhits" falsevalue="" checked="False" | |
245 label="Show all, not just top hits" help="(--uc_allhits)"/> | |
246 </when> | |
247 <when value="no" /> | |
248 </conditional> | |
249 | |
250 <param name="wordlength" type="integer" value="8" min="3" max="15" label="Length of words for database index 3-15" | |
251 help="Allowed values: 3-15 (--wordlength)"/> | |
252 <param name="output_no_hits" type="boolean" truevalue="--output_no_hits" falsevalue="" checked="False" | |
253 label="Write non-matching queries to the output file" help="(--output_no_hits)"/> | |
254 | |
255 <expand macro="userfields" /> | |
256 </when> | |
257 </conditional> | |
258 | |
259 | |
260 </inputs> | |
261 <outputs> | |
262 <data name="uc" format="fasta" label="${tool.name} on ${on_string}: UCLUST like output"> | |
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263 <filter>adv_opts['adv_opts_selector'] == "advanced" and adv_opts['uclust_output']['uclust_output_select'] == 'yes'</filter> |
0 | 264 </data> |
265 <data name="dbnotmatched" format="fasta" label="${tool.name} on ${on_string}: Non-matched database sequences"> | |
266 <filter>'--dbnotmatchedt' in outputs</filter> | |
267 </data> | |
268 <data name="dbmatched" format="fasta" label="${tool.name} on ${on_string}: Matched database sequences"> | |
269 <filter>'--dbmatched' in outputs</filter> | |
270 </data> | |
271 <data name="alnout" format="fasta" label="${tool.name} on ${on_string}: Alignment"> | |
272 <filter>'--alnout' in outputs</filter> | |
273 </data> | |
274 <data name="blast6out" format="tabular" label="${tool.name} on ${on_string}: BLAST like tabular"> | |
275 <filter>'--blast6out' in outputs</filter> | |
276 </data> | |
277 <data name="notmatched" format="fasta" label="${tool.name} on ${on_string}: Non-matched queries"> | |
278 <filter>'--notmatched' in outputs</filter> | |
279 </data> | |
280 <data name="matched" format="fasta" label="${tool.name} on ${on_string}: Matching query sequences"> | |
281 <filter>'--matched' in outputs</filter> | |
282 </data> | |
283 <data name="fastapairs" format="fasta" label="${tool.name} on ${on_string}: Query/Target sequences"> | |
284 <filter>'--fastapairs' in outputs</filter> | |
285 </data> | |
286 <data name="userout" format="tabular" label="${tool.name} on ${on_string}: tabular output"> | |
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287 <filter>adv_opts['adv_opts_selector'] == "advanced" and adv_opts['userfields_output']['userfields_output_select'] == 'yes'</filter> |
0 | 288 </data> |
289 </outputs> | |
290 <tests> | |
291 <test> | |
292 <param name="dbfile" value="three_human_mRNA.fasta" ftype="fasta" /> | |
293 <param name="queryfile" value="query.fasta" ftype="fasta" /> | |
294 <param name="outputs" value="--blast6out,--dbmatched" /> | |
295 <param name="dbmask" value="none" /> | |
296 <param name="adv_opts_selector" value="advanced" /> | |
297 <param name="top_hits_only" value="True" /> | |
298 <output name="dbmatched" file="search_dbmatched_result1.fasta" ftype="fasta" /> | |
299 <output name="blast6out" file="search_blast6out_result1.tabular" ftype="tabular" /> | |
300 </test> | |
301 <test> | |
302 <param name="dbfile" value="three_human_mRNA.fasta" ftype="fasta" /> | |
303 <param name="queryfile" value="query.fasta" ftype="fasta" /> | |
304 <param name="outputs" value="--fastapairs,--matched" /> | |
305 <param name="dbmask" value="none" /> | |
306 <param name="adv_opts_selector" value="advanced" /> | |
307 <param name="userfields_output_select" value="yes" /> | |
308 <param name="userfields" value="query,target,evalue" /> | |
309 <param name="top_hits_only" value="True" /> | |
310 <param name="output_no_hits" value="True" /> | |
311 <output name="matched" file="search_matched_result2.fasta" ftype="fasta" /> | |
312 <output name="fastapairs" file="search_fastapairs_result2.fasta" ftype="fasta" /> | |
313 <output name="userout" file="search_userfields_result2.tabular" ftype="tabular" /> | |
314 </test> | |
315 </tests> | |
316 <help> | |
317 <![CDATA[ | |
318 **What it does** | |
319 | |
320 Sequence search based on vsearch. | |
321 | |
322 @USERFIELDS_HELP@ | |
323 | |
324 Searching options | |
325 --alnout FILENAME filename for human-readable alignment output | |
326 --blast6out FILENAME filename for blast-like tab-separated output | |
327 --db FILENAME filename for FASTA formatted database for search | |
328 --dbmask mask db with "dust", "soft" or "none" method (dust) | |
329 --dbmatched FILENAME FASTA file for matching database sequences | |
330 --dbnotmatched FILENAME FASTA file for non-matching database sequences | |
331 --fastapairs FILENAME FASTA file with pairs of query and target | |
332 --fulldp full dynamic programming alignment (always on) | |
333 --gapext STRING penalties for gap extension (2I/1E) | |
334 --gapopen STRING penalties for gap opening (20I/2E) | |
335 --hardmask mask by replacing with N instead of lower case | |
336 --id REAL reject if identity lower | |
337 --iddef INT id definition, 0-4=CD-HIT,all,int,MBL,BLAST (2) | |
338 --idprefix INT reject if first n nucleotides do not match | |
339 --idsuffix INT reject if last n nucleotides do not match | |
340 --leftjust reject if terminal gaps at alignment left end | |
341 --match INT score for match (2) | |
342 --matched FILENAME FASTA file for matching query sequences | |
343 --maxaccepts INT number of hits to accept and show per strand (1) | |
344 --maxdiffs INT reject if more substitutions or indels | |
345 --maxgaps INT reject if more indels | |
346 --maxhits INT maximum number of hits to show (unlimited) | |
347 --maxid REAL reject if identity higher | |
348 --maxqsize INT reject if query abundance larger | |
349 --maxqt REAL reject if query/target length ratio higher | |
350 --maxrejects INT number of non-matching hits to consider (32) | |
351 --maxsizeratio REAL reject if query/target abundance ratio higher | |
352 --maxsl REAL reject if shorter/longer length ratio higher | |
353 --maxsubs INT reject if more substitutions | |
354 --mid REAL reject if percent identity lower, ignoring gaps | |
355 --mincols INT reject if alignment length shorter | |
356 --minqt REAL reject if query/target length ratio lower | |
357 --minsizeratio REAL reject if query/target abundance ratio lower | |
358 --minsl REAL reject if shorter/longer length ratio lower | |
359 --mintsize INT reject if target abundance lower | |
360 --mismatch INT score for mismatch (-4) | |
361 --notmatched FILENAME FASTA file for non-matching query sequences | |
362 --output_no_hits output non-matching queries to output files | |
363 --qmask mask query with "dust", "soft" or "none" method (dust) | |
364 --query_cov REAL reject if fraction of query seq. aligned lower | |
365 --rightjust reject if terminal gaps at alignment right end | |
366 --rowlen INT width of alignment lines in alnout output (64) | |
367 --self reject if labels identical | |
368 --selfid reject if sequences identical | |
369 --sizeout write abundance annotation to output | |
370 --strand search "plus" or "both" strands (plus) | |
371 --target_cov REAL reject if fraction of target seq. aligned lower | |
372 --top_hits_only output only hits with identity equal to the best | |
373 --uc FILENAME filename for UCLUST-like output | |
374 --uc_allhits show all, not just top hit with uc output | |
375 --usearch_global FILENAME filename of queries for global alignment search | |
376 --userfields STRING fields to output in userout file | |
377 --userout FILENAME filename for user-defined tab-separated output | |
378 --weak_id REAL include aligned hits with >= id; continue search | |
379 --wordlength INT length of words for database index 3-15 (8) | |
380 | |
381 @EXTERNAL_DOCUMENTATION@ | |
382 | |
383 | |
384 ]]> | |
385 </help> | |
386 <expand macro="citations" /> | |
387 </tool> |