Mercurial > repos > iuc > vsnp_get_snps

annotate vsnp_get_snps.xml @ 0:ec6e02f4eab7 draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
author iuc
date Tue, 16 Nov 2021 08:26:58 +0000
parents
children 9ac0b1d5560d
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ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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1 <tool id="vsnp_get_snps" name="vSNP: get SNPs" version="@WRAPPER_VERSION@.0" profile="@PROFILE@">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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2 <description></description>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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6 <requirements>
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7 <requirement type="package" version="3.0.9">openpyxl</requirement>
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8 <requirement type="package" version="1.3.4">pandas</requirement>
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9 <requirement type="package" version="0.6.8">pyvcf</requirement>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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10 <requirement type="package" version="2.0.1">xlrd</requirement>
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11 </requirements>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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12 <command detect_errors="exit_code"><![CDATA[
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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13 #import re
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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14
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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15 #set input_vcf_dir = 'input_vcf_dir'
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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16 #set output_json_avg_mq_dir = 'output_json_avg_mq_dir'
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17 #set output_json_snps_dir = 'output_json_snps_dir'
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18 #set output_snps_dir = 'output_snps_dir'
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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19
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20 mkdir -p $input_vcf_dir &&
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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21 mkdir -p $output_json_avg_mq_dir &&
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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22 mkdir -p $output_json_snps_dir &&
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23 mkdir -p $output_snps_dir &&
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24
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25 #set dbkey = '?'
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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26 #for $i in $input_vcf_collection:
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27 #if str($dbkey) == '?':
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28 #set dbkey = $i.metadata.dbkey
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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29 #else if str($dbkey) != $i.metadata.dbkey:
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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30 >&2 echo "The dbkeys associated with the zero coverage VCF files with SNPs found in closely related isolate groups are not unique" &&
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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31 exit 1
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32 #end if
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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33 #set vcf_identifier = re.sub('[^\s\w\-]', '_', str($i.element_identifier))
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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34 ln -s '${i}' '$input_vcf_dir/${vcf_identifier}' &&
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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35 #end for
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36 #if str($dbkey) == '?':
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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37 >&2 echo "The dbkey must be set for the zero coverage VCF files with SNPs found in closely related isolate groups" && exit 1
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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38 #end if
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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39 #if str($input_zc_vcf_type_cond.input_zc_vcf_type) == "single":
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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40 #set zc_vcf_identifier = re.sub('[^\s\w\-]', '_', str($input_zc_vcf.element_identifier))
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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41 ln -s '${input_zc_vcf}' '$input_vcf_dir/${zc_vcf_identifier}' &&
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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42 #else
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43 #for $i in $input_zc_vcf_type_cond.input_zc_vcf_collection:
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44 #set zc_vcf_identifier = re.sub('[^\s\w\-]', '_', str($i.element_identifier))
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45 ln -s '${i}' '$input_vcf_dir/${zc_vcf_identifier}' &&
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46 #end for
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47 #end if
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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48 #if str($input_excel_cond.input_excel_param) == 'yes':
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49 #if str($input_excel_cond.excel_source_cond.excel_source) == 'cached':
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50 #set excel_file = 'No genome specified for input VCF (database) file(s)'
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51 #set excel_fields = $__app__.tool_data_tables['vsnp_excel'].get_fields()
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52 ## The value of excel_fields is a nested list that looks like this.
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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53 ## [['AF2122', 'Mbovis_define_filter.xlsx', '~/tool-data/vsnp/AF2122/excel/Mbovis_define_filter.xlsx', 'Excel file for AF2122'],...]
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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54 #for $i in $excel_fields:
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55 #if str($i[0]) == $dbkey:
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56 #set excel_file = $i[2]
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57 #break
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58 #end if
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59 #end for
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60 #else:
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61 #set excel_file = $input_excel_cond.excel_source_cond.input_excel
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62 #end if
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63 #end if
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64 python '$__tool_directory__/vsnp_get_snps.py'
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65 --ac $ac
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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66 #if str($input_excel_cond.input_excel_param) == 'yes':
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67 --input_excel '$excel_file'
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68 #end if
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69 $all_isolates
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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70 --input_vcf_dir '$input_vcf_dir'
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71 --min_mq $min_mq
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72 --min_quality_score $min_quality_score
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73 --output_json_avg_mq_dir '$output_json_avg_mq_dir'
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74 --output_json_snps_dir '$output_json_snps_dir'
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75 --output_snps_dir '$output_snps_dir'
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76 --output_summary '$output_summary'
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77 --processes \${GALAXY_SLOTS:-8}
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78 --quality_score_n_threshold $quality_score_n_threshold
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79 --dbkey '$dbkey'
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80 ]]></command>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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81 <inputs>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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82 <conditional name="input_zc_vcf_type_cond">
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83 <param name="input_zc_vcf_type" type="select" label="Choose the category of the files to be analyzed">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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84 <option value="collection" selected="true">A collection of zero coverage VCF files</option>
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85 <option value="single">A single zero coverage VCF file</option>
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86 </param>
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87 <when value="single">
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88 <param name="input_zc_vcf" type="data" format="vcf" label="Zero coverage VCF file"/>
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89 </when>
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90 <when value="collection">
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91 <param name="input_zc_vcf_collection" format="vcf" type="data_collection" collection_type="list" label="Collection of zero coverage VCF files"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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92 </when>
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93 </conditional>
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94 <param name="input_vcf_collection" format="vcf" type="data_collection" collection_type="list" label="Collection of zero coverage VCF files with SNPs found in closely related isolate groups"/>
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95 <param name="ac" type="integer" min="0" value="2" label="Allele count threshold" help="At least 1 position must have this value for a SNP to be added to a group"/>
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96 <param name="min_mq" type="integer" min="0" value="56" label="Map quality threshold" help="At least 1 position must have a higher MQ value for a SNP to be added to a group"/>
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97 <param name="min_quality_score" type="integer" min="0" value="150" label="Quality score threshold" help="At least 1 position must have a higher quality score for a SNP to be added to a group"/>
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98 <param name="quality_score_n_threshold" type="integer" min="0" value="150" label="Minimum quality score N value for alleles" help="Alleles are marked as N for quality scores between this value and the minimum quality score value above"/>
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99 <conditional name="input_excel_cond">
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100 <param name="input_excel_param" type="select" label="Use Excel file for grouping and filtering?">
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101 <option value="yes" selected="true">Yes</option>
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102 <option value="no">No</option>
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103 </param>
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104 <when value="yes">
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105 <conditional name="excel_source_cond">
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106 <param name="excel_source" type="select" label="Choose the source for the Excel file">
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107 <option value="cached">locally cached</option>
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108 <option value="history">from history</option>
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109 </param>
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110 <when value="cached">
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111 <param name="input_excel" type="select" label="Excel file">
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112 <options from_data_table="vsnp_excel">
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113 <filter type="data_meta" column="0" key="dbkey" ref="input_vcf_collection"/>
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114 <validator type="no_options" message="No built-in Excel grouping and filtering datasets are available"/>
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115 </options>
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116 </param>
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117 </when>
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118 <when value="history">
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119 <param name="input_excel" type="data" format="xlsx" label="Excel file"/>
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120 </when>
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121 </conditional>
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122 </when>
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123 <when value="no"/>
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124 </conditional>
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125 <param name="all_isolates" type="boolean" truevalue="--all_isolates" falsevalue="" checked="false" label="Create a group containing all isolates?"/>
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126 </inputs>
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127 <outputs>
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128 <collection name="snps" type="list" label="${tool.name} on ${on_string} (SNPs)">
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129 <discover_datasets pattern="__name_and_ext__" directory="output_snps_dir"/>
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130 </collection>
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131 <collection name="json_avg_mq" type="list" label="${tool.name} on ${on_string} (average mq)">
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132 <discover_datasets pattern="__name_and_ext__" directory="output_json_avg_mq_dir"/>
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133 </collection>
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134 <collection name="json_snps" type="list" label="${tool.name} on ${on_string} (SNPs as json)">
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135 <discover_datasets pattern="__name_and_ext__" directory="output_json_snps_dir"/>
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136 </collection>
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137 <data name="output_summary" format="html" label="${tool.name} on ${on_string} (summary)"/>
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138 </outputs>
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139 <tests>
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140 <!--
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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141 Unfortunately the test files cannot be gzipped since Galaxy changes the file names
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142 to be something like 00-0121_WI_Cervid_99-A_vcf_gz, and the VCF Reader requires
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143 gzipped files to have a .gz extension. The exception is
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144 UnicodeDecodeError: 'utf-8' codec can't decode byte 0x8b in position 1: invalid start byte
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145 -->
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146 <!-- A single vcf input, no excel file, all_isolates is False -->
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147 <test expect_num_outputs="4">
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148 <param name="input_zc_vcf_type" value="single"/>
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149 <param name="input_zc_vcf" value="input_zc_vcf.vcf" ftype="vcf" dbkey="89"/>
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150 <param name="input_vcf_collection">
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151 <collection type="list">
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152 <element name="SRR8073662_zc.vcf" value="SRR8073662_zc.vcf" dbkey="89"/>
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153 <element name="SRR1792272_zc.vcf" value="SRR1792272_zc.vcf" dbkey="89"/>
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154 </collection>
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155 </param>
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156 <param name="input_excel_param" value="no"/>
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157 <output_collection name="snps" type="list" count="1">
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158 <element name="all_vcf" ftype="fasta">
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159 <assert_contents>
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160 <has_size value="150"/>
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161 </assert_contents>
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162 </element>
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163 </output_collection>
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164 <output_collection name="json_avg_mq" type="list" count="1">
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165 <element name="all_vcf" ftype="json">
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166 <assert_contents>
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167 <has_size value="551"/>
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168 </assert_contents>
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diff changeset
169 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
170 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
171 <output_collection name="json_snps" type="list" count="1">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
172 <element name="all_vcf" ftype="json">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
173 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
174 <has_size value="876"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
175 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
176 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
177 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
178 <output name="output_summary" ftype="html">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
179 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
180 <has_size value="303"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
181 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
182 </output>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
183 </test>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
184 <!-- An input collection, no excel file, all_isolates is False -->
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
185 <test expect_num_outputs="4">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
186 <param name="input_zc_vcf_type" value="collection"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
187 <param name="input_zc_vcf_collection">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
188 <collection type="list">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
189 <element name="BCG_Pasteur_Unknown_FR_SRR8886989.vcf" value="BCG_Pasteur_Unknown_FR_SRR8886989.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
190 <element name="BCG_Tokyo_Unknown_JP_DRR029468.vcf" value="BCG_Tokyo_Unknown_JP_DRR029468.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
191 </collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
192 </param>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
193 <param name="input_vcf_collection">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
194 <collection type="list">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
195 <element name="01_1787_FL_Zoo_Jaguar.vcf" value="01_1787_FL_Zoo_Jaguar.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
196 <element name="02_5877_MEX_TX_Fed.vcf" value="02_5877_MEX_TX_Fed.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
197 <element name="02_0585_COA_TX_Fed.vcf" value="02_0585_COA_TX_Fed.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
198 </collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
199 </param>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
200 <param name="input_excel_param" value="no"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
201 <output_collection name="snps" type="list" count="1">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
202 <element name="all_vcf" ftype="fasta">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
203 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
204 <has_size value="5226"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
205 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
206 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
207 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
208 <output_collection name="json_avg_mq" type="list" count="1">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
209 <element name="all_vcf" ftype="json">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
210 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
211 <has_size value="24332"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
212 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
213 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
214 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
215 <output_collection name="json_snps" type="list" count="1">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
216 <element name="all_vcf" ftype="json">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
217 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
218 <has_size value="38798"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
219 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
220 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
221 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
222 <output name="output_summary" ftype="html">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
223 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
224 <has_size value="303"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
225 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
226 </output>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
227 </test>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
228 <!-- An input collection, an excel file, all_isolates is False -->
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
229 <test expect_num_outputs="4">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
230 <param name="input_zc_vcf_type" value="collection"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
231 <param name="input_zc_vcf_collection">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
232 <collection type="list">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
233 <element name="BCG_Pasteur_Unknown_FR_SRR8886989.vcf" value="BCG_Pasteur_Unknown_FR_SRR8886989.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
234 <element name="BCG_Tokyo_Unknown_JP_DRR029468.vcf" value="BCG_Tokyo_Unknown_JP_DRR029468.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
235 </collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
236 </param>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
237 <param name="input_vcf_collection">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
238 <collection type="list">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
239 <element name="01_1787_FL_Zoo_Jaguar.vcf" value="01_1787_FL_Zoo_Jaguar.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
240 <element name="02_5877_MEX_TX_Fed.vcf" value="02_5877_MEX_TX_Fed.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
241 <element name="02_0585_COA_TX_Fed.vcf" value="02_0585_COA_TX_Fed.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
242 </collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
243 </param>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
244 <param name="input_excel_param" value="yes"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
245 <param name="input_excel" value="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
246 <output_collection name="snps" type="list" count="1">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
247 <element name="Mbovis-17" ftype="fasta">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
248 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
249 <has_size value="749"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
250 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
251 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
252 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
253 <output_collection name="json_avg_mq" type="list" count="1">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
254 <element name="Mbovis-17" ftype="json">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
255 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
256 <has_size value="10884"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
257 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
258 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
259 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
260 <output_collection name="json_snps" type="list" count="1">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
261 <element name="Mbovis-17" ftype="json">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
262 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
263 <has_size value="6396"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
264 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
265 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
266 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
267 <output name="output_summary" ftype="html">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
268 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
269 <has_size value="1057"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
270 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
271 </output>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
272 </test>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
273 <!-- An input collection, an excel file, all_isolates is True -->
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
274 <test expect_num_outputs="4">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
275 <param name="input_zc_vcf_type" value="collection"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
276 <param name="input_zc_vcf_collection">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
277 <collection type="list">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
278 <element name="BCG_Pasteur_Unknown_FR_SRR8886989.vcf" value="BCG_Pasteur_Unknown_FR_SRR8886989.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
279 <element name="BCG_Tokyo_Unknown_JP_DRR029468.vcf" value="BCG_Tokyo_Unknown_JP_DRR029468.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
280 </collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
281 </param>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
282 <param name="input_vcf_collection">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
283 <collection type="list">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
284 <element name="01_1787_FL_Zoo_Jaguar.vcf" value="01_1787_FL_Zoo_Jaguar.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
285 <element name="02_5877_MEX_TX_Fed.vcf" value="02_5877_MEX_TX_Fed.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
286 <element name="02_0585_COA_TX_Fed.vcf" value="02_0585_COA_TX_Fed.vcf" dbkey="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
287 </collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
288 </param>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
289 <param name="input_excel_param" value="yes"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
290 <param name="input_excel" value="89"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
291 <param name="all_isolates" value="--all_isolates"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
292 <output_collection name="snps" type="list" count="2">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
293 <element name="Mbovis-17" ftype="fasta">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
294 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
295 <has_size value="749"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
296 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
297 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
298 <element name="all_vcf" ftype="fasta">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
299 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
300 <has_size value="4920"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
301 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
302 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
303 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
304 <output_collection name="json_avg_mq" type="list" count="2">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
305 <element name="Mbovis-17" ftype="json">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
306 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
307 <has_size value="10884"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
308 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
309 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
310 <element name="all_vcf" ftype="json">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
311 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
312 <has_size value="24332"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
313 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
314 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
315 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
316 <output_collection name="json_snps" type="list" count="2">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
317 <element name="Mbovis-17" ftype="json">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
318 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
319 <has_size value="6396"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
320 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
321 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
322 <element name="all_vcf" ftype="json">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
323 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
324 <has_size value="36466"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
325 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
326 </element>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
327 </output_collection>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
328 <output name="output_summary" ftype="html">
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
329 <assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
330 <has_size value="1056"/>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
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diff changeset
331 </assert_contents>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
332 </output>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
333 </test>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
334 </tests>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
335 <help>
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
336 **What it does**
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
337
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
338 Accepts a zero coverage VCF file produced by the **vSNP: add zero coverage** tool (or a collection of them) along with a collection
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
339 of zero coverage VCF files that have been aligned with the same reference and contain SNPs called between closely related isolate groups.
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
340 The tool produces fasta files containing SNP alignments, json files containing the SNP positions and additional json files containing
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
341 the average map quality values.
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
342
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
343 The SNP alignments produced by this tool are used to create phylogenetic trees, so larger input collections result in more populated
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
diff changeset
344 phylogenetic trees. Both of the json outputs are used by the **vSNP: build tables** tool to produce annotated SNP tables in the form
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
iuc
parents:
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345 of Excel spreadsheets.
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346
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347 An Excel spreadsheet containing specified SNPs can optiomally be used to filter desired SNP positions by group. Users can choose a
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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348 locally cached Excel spreadsheet or one from their current history.
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349
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350 A SNP is added to a group if it has at least one position with a specified allele count value, a quality score greater than a specified
ec6e02f4eab7 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 95b221f68d19702681babd765c67caeeb24e7f1d"
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351 value, and a map quality greater than a specified value.
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352
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353 If the allele count equals the specified value (2) and the quality score for a SNP position is greater than the minimum quality score
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354 value (150), the alternate allele is called.
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355
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356 However, if the allele count is 1, the position is called ambiguous. Deletions are called when the alternate allele is a gap. If the
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357 quality score is less than or equal to the minimum quality score N value for alleles (150), the allele is marked "N".
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358
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359 **Required Options**
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360
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361 * **Zero coverage VCF file(s)** - Select a single or collection of zero coverage VCF files, typically produced by the **vSNP: add zero coverage** tool, from the current history.
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362 * **Collection of zero coverage VCF files with SNPs found in closely related isolate groups** - Select a dataset collection of zero coverage vcf files from the current history.
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363
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364 **Additional Options**
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365
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366 * **Allele count threshold** - At least 1 position must have an allele count greater than this value for a SNP to be added to a group (2 is optimal).
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367 * **Map quality threshold** - At least 1 position must have a higher MQ value for a SNP to be added to a group (56 is optimal).
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368 * **Quality score threshold** -At least 1 position must have a higher quality score for a SNP to be added to a group (150 is optimal).
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369 * **Minimum quality score N value for alleles** - If none of the avove 3 requirements is met and the quality score is less than or equal to the minimum quality score N value for alleles, the allele is marked "N" (150 is optimal).
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370 * **Use Excel file for grouping and filtering?** - select Yes to filter desired SNP positions by group. A cached Excel spreadsheet provides the most widely used SNP positions for grouping, but a custom spreadhseet can be selected from the current history.
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371 * **Create a group containing all isolates?** - select Yes to output an additional group containing of all isolates.
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372 </help>
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373 <expand macro="citations"/>
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374 </tool>
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375