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"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
author | jjohnson |
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date | Sun, 21 Feb 2021 23:40:09 +0000 |
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children | 4635a93ebd91 |
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<tool id="fgbio_fastq_to_bam" name="fgbio FastqToBam" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" python_template_version="3.5"> <description>Generates an unmapped BAM file from fastq files</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements" /> <version_command>fgbio --version</version_command> <command detect_errors="exit_code"><![CDATA[ fgbio FastqToBam --input #for $input in $inputs '$input' #end for --sample='$sample' --library='$library' #if $read_structures: --read-structures $read_structures #end if --sort='$sort' --output '$output' ## optional bam header content #if $bam_header.umi_tag --umi-tag='$bam_header.umi_tag' #end if #if $bam_header.predicted_insert_size --predicted-insert-size='$bam_header.predicted_insert_size' #end if #if $bam_header.read_group --read-group='$bam_header.read_group' #end if #if $bam_header.description --description='$bam_header.description' #end if #if $bam_header.platform --platform='$bam_header.platform' #end if #if $bam_header.platform_model --platform-model='$bam_header.platform_model' #end if #if $bam_header.platform_model --platform-model='$bam_header.platform_model' #end if #if $bam_header.platform_unit --platform-unit='$bam_header.platform_unit' #end if #if $bam_header.sequencing_center --sequencing-center='$bam_header.sequencing_center' #end if #if $bam_header.comment --comment='$bam_header.comment' #end if ]]></command> <inputs> <param name="inputs" type="data" format="fastq" multiple="true" label="Fastq files corresponding to each sequencing read"/> <param argument="--sample" type="text" value="" label="The name of the sequenced sample"> </param> <param argument="--library" type="text" value="" label="The name/ID of the sequenced library"> </param> <param argument="--read-structures" type="text" value="" optional="true" label="Read structures, one for each of the FASTQ"> <expand macro="read_structures_validator" /> </param> <param argument="--sort" type="boolean" truevalue="true" falsevalue="false" checked="false" label="Sort bam by queryname" help="If true, queryname sort the BAM file, otherwise preserve input order."/> <section name="bam_header" title="BAM Header" expanded="false"> <param argument="--umi-tag" type="text" value="" optional="true" label="Tag in which to store molecular barcodes/UMIs" help="Default: RX"> <expand macro="sam_tag_validator" /> </param> <param argument="--predicted-insert-size" type="integer" value="" optional="true" label="Predicted median insert size, to insert into the read group header"/> <param argument="--read-group" type="text" value="" optional="true" label="Read group ID to use in the file header" help="Default: A"/> <param argument="--description" type="text" value="" optional="true" label="Description of the read group"/> <param argument="--platform" type="text" value="" optional="true" label="Sequencing Platform" help="Default: illumina"/> <param argument="--platform-model" type="text" value="" optional="true" label="Platform model to insert into the group header (ex. miseq, hiseq2500, hiseqX)"/> <param argument="--platform-unit" type="text" value="" optional="true" label="Platform unit (e.g. 'flowcell-barcode.lane.sample-barcode')"/> <param argument="--sequencing-center" type="text" value="" optional="true" label="The sequencing center from which the data originated"/> <param argument="--comment" type="text" value="" optional="true" label="Comment to include in the output header"/> </section> </inputs> <outputs> <data name="output" format="unsorted.bam" /> </outputs> <help><![CDATA[ **fgbio FastqToBam** Generates an unmapped BAM (or SAM or CRAM) file from fastq files. Takes in one or more fastq files (optionally gzipped), each representing a different sequencing read (e.g. R1, R2, I1 or I2) and can use a set of read structures to allocate bases in those reads to template reads, sample indices, unique molecular indices, or to designate bases to be skipped over. @READ_STRUCTURES_HELP@ http://fulcrumgenomics.github.io/fgbio/tools/latest/FastqToBam.html ]]></help> <expand macro="citations" /> </tool>