Mercurial > repos > joachim-jacob > qualimap_suite
view bamqc_wrapper.pl @ 5:f6ffb9aa409e draft default tip
Adding some more info
author | Joachim Jacob <joachim.jacob@gmail.com> |
---|---|
date | Wed, 20 Mar 2013 09:41:43 +0100 |
parents | 3d690162d629 |
children |
line wrap: on
line source
#!/usr/bin/perl # bamqc_wrapper.pl # Joachim Jacob - joachim.jacob@gmail.com - 2013 use strict; use File::Temp 'tempdir'; use File::Basename; use Log::Log4perl qw(:easy); # ---------------------- Prepping Logging -----------------------------# ######################################################################## # Log levels: $DEBUG $INFO $WARN $ERROR $FATAL # ConversionPattern: %d %-5p %F{1} [%M] (line %L): %m%n%n my $log_conf = q/ log4perl.category = ERROR, Screen log4perl.appender.Screen.stderr=1 log4perl.appender.Screen.layout=Log::Log4perl::Layout::PatternLayout log4perl.appender.Screen.layout.ConversionPattern = %d %-5p %m%n log4perl.appender.Screen = Log::Log4perl::Appender::Screen /; Log::Log4perl::init( \$log_conf ); my $logger = get_logger(); # ----------------- Getting parameters file ---------------------------# ######################################################################## my ($configfile) = @ARGV; my (%para); open(CONFIG,"<$configfile"); while (<CONFIG>) { if (/(\S+)==(.+)$/){ $para{ $1 } = $2 ; } } close(CONFIG); =Excerpt Config parameters ## first we pass some galaxy environment variables galtemp==${__new_file_path__} bam==$bam c==$c hm==$hm nr==$nr #if $customgtf.upload=="yes" gff==$customgtf.gff os==$customgtf.os p==$customgtf.p #end if =cut for my $para (keys %para){ INFO "$para\tset to\t$para{$para}"; } # ---------------------- Prepping temp dir ----------------------------# ######################################################################## # within the temporary directory of Galaxy, we create a temporary dir my $galtemp = $para{'galtemp'}; DEBUG "\nReceived Galaxy temporary directory:\n$galtemp"; my $tempdir = File::Temp->tempdir('tmpXXXXX', DIR => $galtemp, CLEANUP => 1); mkdir "$tempdir", 077 unless -d "$tempdir"; INFO "\nTemporary directory:\n$tempdir"; # -------------------- Assembling command ----------------------------# ######################################################################## my $outdir="bamqc_output"; my $qualimap_path=""; # for tool_dependency later on my $command = $qualimap_path."qualimap bamqc --outdir $outdir"; # this will ultimately be executed # based on parameters, which are called by $para{'parametername'} # the command is assembled $command .= " -bam $para{'bam'} -hm $para{'hm'} -nr $para{'nr'}"; if($para{'c'}){$command .= " $para{'c'} "; } if ( $para{'gff'} ){ $command .= " -gff $para{'gff'} -p $para{'p'} "; if($para{'os'}) { $command .= $para{'os'}; } } $command .= " -nt 4 -outformat HTML"; # --------------------- Executing command ----------------------------# ######################################################################## run_process($command, "qualimap bamqc", "."); # ----------------- Postprocessing command ---------------------------# ######################################################################## # Hackish !! my $librarydir = dirname($para{'bamqc_result'}); my $jobworkdir = dirname($para{'outputdir'}); my $files_path_name = basename($para{'outputdir'}); INFO "\n Librarydir = $librarydir \n jobworkdir = $jobworkdir \n files_path_name = $files_path_name \n"; system("mv $jobworkdir/bamqc_output $librarydir/$files_path_name") == 0 or die "Could not move output data to $librarydir\n"; system("rm -rf $para{'bamqc_result'}") == 0 or die "Could not post-process the results...\n"; system("ln -s $librarydir/$files_path_name/qualimapReport.html $para{'bamqc_result'}") == 0 or die "Could not move output data to $librarydir\n"; # --------------------------- Exiting --------------------------------# ######################################################################## exit 0; ### Subroutines ### ######################################################################## sub run_process { my ($command, $name, $tempdir)= @_; my $logger = get_logger(); INFO "\nProcess to launch:\n $command\nIn directory\n$tempdir\n"; system("cd $tempdir; $command 2>/dev/null") == 0 or die "$name failed\nExit status $?\nCommand: $command"; if ($? == -1) { print "failed to execute: $!\n"; } elsif ($? & 127) { printf "child died with signal %d, %s coredump\n", ($? & 127), ($? & 128) ? 'with' : 'without'; } else { printf "$name executed successfully\n", $? >> 8; } }