Mercurial > repos > jvolkening > centrifuge
annotate centrifuge.xml @ 0:be7bba8229c6 draft default tip
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
author | jvolkening |
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date | Fri, 01 Nov 2019 13:53:15 -0400 |
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be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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1 <tool id="centrifuge" name="Centrifuge" version="1.0.4_beta"> |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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2 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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3 <description>Read-based metagenome characterization</description> |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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4 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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5 <!-- ***************************************************************** --> |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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6 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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7 <requirements> |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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8 <requirement type="package" version="1.0.4_beta">centrifuge</requirement> |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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9 </requirements> |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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10 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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11 <!-- ***************************************************************** --> |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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12 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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13 <version_command>centrifuge --version | perl -wnE'print "$1\n" for /centrifuge\S+ version (\S+)/g'</version_command> |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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14 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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15 <!-- ***************************************************************** --> |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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16 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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17 <command detect_errors="aggressive"> |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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18 <![CDATA[ |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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19 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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20 centrifuge |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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21 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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22 ##--Output Options------------------------------ |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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23 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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24 --out-fmt $outputs.out_fmt |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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25 --tab-fmt-cols $outputs.tab_fmt_cols |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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26 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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27 ##--General Options----------------------------- |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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28 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
jvolkening
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29 --threads \${GALAXY_SLOTS:-1} |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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30 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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31 #if $general.skip != "": |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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32 --skip $general.skip |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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33 #end if |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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34 #if $general.upto != "": |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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35 --upto $general.upto |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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36 #end if |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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37 #if $general.trim5 != "": |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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38 --trim5 $general.trim5 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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39 #end if |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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40 #if $general.trim3 != "": |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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41 --trim3 $general.trim3 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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42 #end if |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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43 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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44 $general.ignore_quals |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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45 $general.nofw |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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46 $general.norc |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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47 $general.non_deterministic |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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48 |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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49 #if $general.seed != "": |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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50 --seed $general.seed |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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51 #end if |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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52 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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53 ##--Classification------------------------------ |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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54 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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55 --min-hitlen $classification.min_hitlen |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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56 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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57 #if $classification.min_totallen != "": |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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58 --min-totallen $classification.min_totallen |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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59 #end if |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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60 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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61 #if $classification.host_taxids != "": |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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62 --host-taxids $classification.host_taxids |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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63 #end if |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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64 |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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65 #if $classification.exclude_taxids != "": |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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66 --exclude-taxids $classification.exclude_taxids |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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67 #end if |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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68 |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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69 ##--Inputs-------------------------------------- |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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70 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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71 -x '${inputs.db.fields.path}' |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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72 |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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73 #for $s in $inputs.unpaired |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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74 -U '${s.u_reads}' |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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75 #end for |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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76 |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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77 #for $s in $inputs.paired |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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78 -1 '${s.p_reads.forward}' |
be7bba8229c6
planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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79 -2 '${s.p_reads.reverse}' |
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80 #end for |
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81 |
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82 #if $inputs.sra: |
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83 --sra-acc $inputs.sra |
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84 #end if |
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85 |
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86 #if $outputs.out_fmt == "tab": |
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87 -S $out_tab |
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88 #elif $outputs.out_fmt == "sam": |
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89 -S $out_sam |
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90 #end if |
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91 |
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92 --report-file $report |
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93 |
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94 ]]> |
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95 </command> |
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96 |
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97 <!-- ***************************************************************** --> |
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98 |
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99 <inputs> |
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100 |
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101 <section name="inputs" title="Inputs" expanded="True"> |
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102 |
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103 <repeat name="unpaired" title="Unpaired reads" min="0" default="0"> |
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104 <param name="u_reads" type="data" format="fastq" label="Unpaired reads"/> |
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105 </repeat> |
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106 |
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107 <repeat name="paired" title="Paired reads" min="0" default="0"> |
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108 <param name="p_reads" type="data_collection" collection_type="paired" format="fastq" label="Paired read collection"/> |
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109 </repeat> |
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110 |
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111 <param name="sra" type="text" label="SRA accession"> |
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112 <sanitizer invalid_char=""> |
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113 <valid initial="string.letters,string.digits"> |
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114 <add value="," /> |
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115 </valid> |
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116 </sanitizer> |
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117 </param> |
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118 |
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119 <param name="db" type="select" label="Select a reference database"> |
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120 <options from_data_table="centrifuge_indices"> |
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121 <filter type="sort_by" column="2"/> |
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122 <validator type="no_options" message="No indexes are available for the selected input dataset"/> |
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123 </options> |
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124 </param> |
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125 |
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126 </section> |
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127 |
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128 <section name="outputs" title="Outputs" expanded="False"> |
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129 |
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130 <param argument="--out-fmt" name="out_fmt" type="select" label="Output format"> |
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131 <option value="tab" selected="true">tabular</option> |
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132 <option value="sam">SAM</option> |
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133 </param> |
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134 |
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135 <param argument="--tab-fmt-cols" name="tab_fmt_cols" type="text" label="Output columns" value="readID,seqID,taxID,score,2ndBestScore,hitLength,queryLength,numMatches"> |
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136 <sanitizer invalid_char=""> |
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137 <valid initial="string.letters,string.digits"> |
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138 <add value="," /> |
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139 </valid> |
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140 </sanitizer> |
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141 </param> |
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142 |
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143 </section> |
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144 |
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145 <section name="general" title="General options" expanded="False"> |
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146 |
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147 <param argument="--skip" type="integer" value="" optional="true" label="Initial reads to skip" /> |
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148 <param argument="--upto" type="integer" value="" optional="true" label="Stop after reads" /> |
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149 <param argument="--trim5" type="integer" value="" optional="true" label="Trim 5' bases" /> |
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150 <param argument="--trim3" type="integer" value="" optional="true" label="Trim 3' bases" /> |
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151 |
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152 <param argument="--ignore-quals" name="ignore_quals" type="boolean" truevalue="--ignore-quals" falsevalue="" checked="no" label="Ignore qualities" /> |
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153 |
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154 <param argument="--nofw" type="boolean" truevalue="--nofw" falsevalue="" checked="no" label="Don't map forward strand" /> |
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155 <param argument="--norc" type="boolean" truevalue="--norc" falsevalue="" checked="no" label="Don't map rev-com strand" /> |
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156 |
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157 <param argument="--seed" type="integer" value="" min="0" optional="true" label="Starting seed" /> |
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158 <param argument="--non-deterministic" name="non_deterministic" type="boolean" truevalue="--non-deterministic" falsevalue="" checked="no" label="Use non-deterministic seeding" /> |
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159 |
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160 </section> |
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161 |
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162 <section name="classification" title="Classification" expanded="True"> |
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163 |
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164 <param argument="--min-hitlen" name="min_hitlen" type="integer" value="22" min="16" label="Minimum hit length" /> |
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165 <param argument="--min-totallen" name="min_totallen" type="integer" optional="true" min="0" label="Minimum summed length" /> |
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166 |
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167 <param argument="--host-taxids" name="host_taxids" type="text" label="Host taxonomic IDs"> |
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168 <sanitizer invalid_char=""> |
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169 <valid initial="string.digits"> |
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170 <add value="," /> |
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171 </valid> |
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172 </sanitizer> |
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173 </param> |
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174 <param argument="--exclude-taxids" name="exclude_taxids" type="text" label="Excluded taxonomic IDs"> |
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175 <sanitizer invalid_char=""> |
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176 <valid initial="string.digits"> |
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177 <add value="," /> |
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178 </valid> |
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179 </sanitizer> |
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180 </param> |
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181 |
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182 </section> |
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183 |
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184 </inputs> |
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185 |
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186 <!-- ***************************************************************** --> |
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187 |
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188 <outputs> |
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189 |
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190 <data name="out_tab" format="tabular" label="Centrifuge on ${on_string}: Output"> |
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191 <filter>(outputs['out_fmt'] == "tab")</filter> |
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192 </data> |
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193 <data name="out_sam" format="sam" label="Centrifuge on ${on_string}: Output"> |
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194 <filter>(outputs['out_fmt'] == "sam")</filter> |
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195 </data> |
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196 <data name="report" format="tabular" label="Centrifuge on ${on_string}: Report" /> |
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197 |
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198 </outputs> |
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199 |
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200 <!-- ***************************************************************** --> |
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201 |
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202 <tests> |
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203 <!-- default unpaired --> |
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204 <test> |
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205 <param name="db" value="test_db" /> |
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206 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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207 <output name="out_tab" file="defaults.tsv" sort="true" /> |
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208 <output name="report" file="defaults.report" /> |
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209 </test> |
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210 <!-- default paired --> |
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211 <test> |
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212 <param name="db" value="test_db" /> |
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213 <param name="p_reads"> |
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214 <collection type="paired"> |
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215 <element name="forward" value="input_f.fq" /> |
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216 <element name="reverse" value="input_r.fq" /> |
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217 </collection> |
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218 </param> |
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219 <output name="out_tab" file="paired.tsv" sort="true" /> |
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220 <output name="report" file="paired.report" /> |
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221 </test> |
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222 <!-- default combined --> |
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223 <test> |
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224 <param name="db" value="test_db" /> |
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225 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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226 <param name="p_reads"> |
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227 <collection type="paired"> |
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228 <element name="forward" value="input_f.fq" /> |
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229 <element name="reverse" value="input_r.fq" /> |
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230 </collection> |
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231 </param> |
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232 <output name="out_tab" file="both.tsv" sort="true" /> |
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233 <output name="report" file="both.report" /> |
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234 </test> |
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235 <!-- exclude IDs --> |
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236 <test> |
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237 <param name="db" value="test_db" /> |
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238 <param name="exclude_taxids" value="9913" /> |
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239 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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240 <output name="out_tab" file="exclude.tsv" sort="true" /> |
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241 <output name="report" file="exclude.report" /> |
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242 </test> |
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243 <!-- specify host --> |
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244 <test> |
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245 <param name="db" value="test_db" /> |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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246 <param name="host_taxids" value="9913" /> |
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247 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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248 <output name="out_tab" file="host.tsv" sort="true" /> |
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249 <output name="report" file="host.report" /> |
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250 </test> |
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251 <!-- minimum length --> |
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252 <test> |
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253 <param name="db" value="test_db" /> |
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254 <param name="min_hitlen" value="83" /> |
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255 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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256 <output name="out_tab" file="minlen83.tsv" sort="true" /> |
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257 <output name="report" file="minlen83.report" /> |
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258 </test> |
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259 <!-- norc --> |
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260 <test> |
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261 <param name="db" value="test_db" /> |
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262 <param name="norc" value="true" /> |
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263 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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264 <output name="out_tab" file="norc.tsv" sort="true" /> |
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265 <output name="report" file="norc.report" /> |
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266 </test> |
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267 <!-- nofw --> |
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268 <test> |
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269 <param name="db" value="test_db" /> |
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270 <param name="nofw" value="true" /> |
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271 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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272 <output name="out_tab" file="nofw.tsv" sort="true" /> |
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273 <output name="report" file="nofw.report" /> |
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274 </test> |
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275 <!-- set seed --> |
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276 <test> |
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277 <param name="db" value="test_db" /> |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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278 <param name="seed" value="123" /> |
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279 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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280 <output name="out_tab" file="seed123.tsv" sort="true" /> |
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281 <output name="report" file="seed123.report" /> |
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282 </test> |
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283 <!-- 5' trim --> |
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284 <test> |
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285 <param name="db" value="test_db" /> |
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planemo upload for repository https://github.com/jvolkening/galaxy-tools/tree/master/tools/centrifuge commit 5d9e0a6e2ad278547892c86f0acded93814a1356-dirty
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286 <param name="trim5" value="10" /> |
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287 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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288 <output name="out_tab" file="trim5_10.tsv" sort="true" /> |
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289 <output name="report" file="trim5_10.report" /> |
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290 </test> |
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291 <!-- 3' trim --> |
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292 <test> |
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293 <param name="db" value="test_db" /> |
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294 <param name="trim3" value="5" /> |
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295 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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296 <output name="out_tab" file="trim3_5.tsv" sort="true" /> |
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297 <output name="report" file="trim3_5.report" /> |
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298 </test> |
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299 <!-- skip start --> |
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300 <test> |
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301 <param name="db" value="test_db" /> |
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302 <param name="skip" value="3" /> |
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303 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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304 <output name="out_tab" file="skip3.tsv" sort="true" /> |
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305 <output name="report" file="skip3.report" /> |
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306 </test> |
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307 <!-- skip end --> |
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308 <test> |
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309 <param name="db" value="test_db" /> |
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310 <param name="upto" value="6" /> |
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311 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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312 <output name="out_tab" file="upto6.tsv" sort="true" /> |
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313 <output name="report" file="upto6.report" /> |
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314 </test> |
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315 <!-- invalid parameter value --> |
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316 <test expect_failure="true"> |
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317 <param name="db" value="test_db" /> |
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318 <param name="tab_fmt_cols" value="FooBar" /> |
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319 <param name="u_reads" ftype="fastq" value="input_u.fq" /> |
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320 </test> |
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321 |
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322 </tests> |
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323 |
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324 <!-- ***************************************************************** --> |
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325 |
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326 <help> |
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327 <![CDATA[ |
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328 |
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329 Overview |
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330 -------- |
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331 |
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332 **Centrifuge** is a very rapid and memory-efficient system for the |
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333 classification of DNA sequences from microbial samples, with better |
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334 sensitivity than and comparable accuracy to other leading systems. The system |
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335 uses a novel indexing scheme based on the Burrows-Wheeler transform (BWT) and |
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336 the Ferragina-Manzini (FM) index, optimized specifically for the metagenomic |
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337 classification problem. Centrifuge requires a relatively small index (e.g., |
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338 4.3 GB for ~4,100 bacterial genomes) yet provides very fast classification |
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339 speed, allowing it to process a typical DNA sequencing run within an hour. |
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340 Together these advances enable timely and accurate analysis of large |
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341 metagenomics data sets on conventional desktop computers. |
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342 |
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343 Usage |
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344 ----- |
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345 |
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346 Following is the manpage for `centrifuge`, which can be linked with the |
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347 options above using the help text. Note that not all options are available in |
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348 the Galaxy wrapper. |
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349 |
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350 :: |
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351 |
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352 centrifuge [options]* -x <cf-idx> {-1 <m1> -2 <m2> | -U <r> | --sra-acc <SRA accession number>} [-S <filename>] [--report-file <report>] |
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353 |
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354 <cf-idx> Index filename prefix (minus trailing .X.cf). |
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355 <m1> Files with #1 mates, paired with files in <m2>. |
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356 Could be gzip'ed (extension: .gz) or bzip2'ed (extension: .bz2). |
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357 <m2> Files with #2 mates, paired with files in <m1>. |
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358 Could be gzip'ed (extension: .gz) or bzip2'ed (extension: .bz2). |
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359 <r> Files with unpaired reads. |
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360 Could be gzip'ed (extension: .gz) or bzip2'ed (extension: .bz2). |
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361 <SRA accession number> Comma-separated list of SRA accession numbers, e.g. --sra-acc SRR353653,SRR353654. |
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362 <filename> File for classification output (default: stdout) |
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363 <report> File for tabular report output (default: centrifuge_report.tsv) |
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364 |
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365 <m1>, <m2>, <r> can be comma-separated lists (no whitespace) and can be |
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366 specified many times. E.g. '-U file1.fq,file2.fq -U file3.fq'. |
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367 |
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368 Options (defaults in parentheses): |
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369 |
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370 Input: |
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371 -q query input files are FASTQ .fq/.fastq (default) |
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372 --qseq query input files are in Illumina's qseq format |
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373 -f query input files are (multi-)FASTA .fa/.mfa |
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374 -r query input files are raw one-sequence-per-line |
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375 -c <m1>, <m2>, <r> are sequences themselves, not files |
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376 -s/--skip <int> skip the first <int> reads/pairs in the input (none) |
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377 -u/--upto <int> stop after first <int> reads/pairs (no limit) |
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378 -5/--trim5 <int> trim <int> bases from 5'/left end of reads (0) |
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379 -3/--trim3 <int> trim <int> bases from 3'/right end of reads (0) |
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380 --phred33 qualities are Phred+33 (default) |
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381 --phred64 qualities are Phred+64 |
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382 --int-quals qualities encoded as space-delimited integers |
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383 --ignore-quals treat all quality values as 30 on Phred scale (off) |
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384 --nofw do not align forward (original) version of read (off) |
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385 --norc do not align reverse-complement version of read (off) |
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386 --sra-acc SRA accession ID |
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387 |
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388 Classification: |
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389 --min-hitlen <int> minimum length of partial hits (default 22, must be greater than 15) |
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390 --min-totallen <int> minimum summed length of partial hits per read (default 0) |
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391 --host-taxids <taxids> comma-separated list of taxonomic IDs that will be preferred in classification |
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392 --exclude-taxids <taxids> comma-separated list of taxonomic IDs that will be excluded in classification |
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393 |
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394 Output: |
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395 --out-fmt <str> define output format, either 'tab' or 'sam' (tab) |
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396 --tab-fmt-cols <str> columns in tabular format, comma separated |
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397 default: readID,seqID,taxID,score,2ndBestScore,hitLength,queryLength,numMatches |
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398 -t/--time print wall-clock time taken by search phases |
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399 --un <path> write unpaired reads that didn't align to <path> |
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400 --al <path> write unpaired reads that aligned at least once to <path> |
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401 --un-conc <path> write pairs that didn't align concordantly to <path> |
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402 --al-conc <path> write pairs that aligned concordantly at least once to <path> |
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403 (Note: for --un, --al, --un-conc, or --al-conc, add '-gz' to the option name, e.g. |
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404 --un-gz <path>, to gzip compress output, or add '-bz2' to bzip2 compress output.) |
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405 --quiet print nothing to stderr except serious errors |
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406 --met-file <path> send metrics to file at <path> (off) |
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407 --met-stderr send metrics to stderr (off) |
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408 --met <int> report internal counters & metrics every <int> secs (1) |
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409 |
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410 Performance: |
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411 -o/--offrate <int> override offrate of index; must be >= index's offrate |
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412 -p/--threads <int> number of alignment threads to launch (1) |
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413 --mm use memory-mapped I/O for index; many 'bowtie's can share |
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414 |
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415 Other: |
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416 --qc-filter filter out reads that are bad according to QSEQ filter |
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417 --seed <int> seed for random number generator (0) |
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418 --non-deterministic seed rand. gen. arbitrarily instead of using read attributes |
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419 --version print version information and quit |
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420 -h/--help print this usage message |
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421 |
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422 ]]> |
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423 </help> |
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424 |
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425 <!-- ***************************************************************** --> |
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426 |
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427 <citations> |
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428 <citation type="doi">10.1101/gr.210641.116</citation> |
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429 </citations> |
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430 |
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431 </tool> |