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12
<table align=center border=0 cellspacing=0 cellpadding=0>
<tr><td valign=top>
<A HREF="/" ONMOUSEOVER="self.status='Go to the EMBOSS home page';return true"><img border=0 src="/gembassy/emboss_explorer/manual/emboss_icon.jpg" alt="" width=150 height=48></a>
</td>
<td align=left valign=middle>
<b><font size="+6">
genret
</font></b>
</td></tr>
</table>
<br>
<p>
<!--END OF HEADER-->
<H2> Function </H2>
Retrieves various gene related information from genome flatfile
<!--
DON'T WRITE ANYTHING HERE.
IT IS DONE FOR YOU.
-->
<H2>Description</H2>
<p>
genret reads in one or more genome flatfiles and retrieves various data from<br />
the input file. It is a wrapper program to the G-language REST service,<br />
where a method is specified by giving a string to the "method" qualifier. By<br />
default, genret will parse the input file to retrieve the accession ID<br />
(or name) of the genome to query G-language REST service. By setting the<br />
"accid" qualifier to false (or 0), genret will instead parse the sequence<br />
and features of the genome to create a GenBank formatted flatfile and upload<br />
the file to the G-language web server. Using the file uploaded, genret will<br />
execute the method provided.<br />
<br />
genret is able to perform a variety of tasks, incluing the retrieval of<br />
sequence upstream, downstream, or around the start or stop codon,<br />
translated gene sequences search of gene data by keyword.<br />
<br />
Details on G-language REST service is available from the wiki page<br />
<br />
http://www.g-language.org/wiki/rest<br />
<br />
Documentation on G-language Genome Analysis Environment methods are<br />
provided at the Document Center<br />
<br />
http://ws.g-language.org/gdoc/<br />
<br />
</p>
<H2>Usage</H2>
Here is a sample session with genret<br><br>
Retrieving sequences upstream, downstream, or around the start/stop codons.
The following example shows the retrieval of sequence around the start
codons of all genes.<br><br>
Genes to access are specified by regular expression. '*' stands for every
gene.<br><br>
Available methods are:<br>
after_startcodon<br>
after_stopcodon<br>
around_startcodon<br>
around_stopcodon<br>
before_startcodon<br>
before_stopcodon<br><br>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% genret
Retrieves various gene related information from genome flatfile
Input nucleotide sequence(s): refseqn:NC_000913
Gene name(s) to lookup [*]:
Feature to access: around_startcodon
Full text output file [nc_000913.around_startcodon]:
</pre></td></tr></table>
Go to the <a href="#input">input files</a> for this example<br>
Go to the <a href="#output">output files</a> for this example<br><br>
Example 2<br><br>
Using flat text as target genes. The names can be split with with a space, comma, or vertical bar.<br><br>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% genret
Retrieves various gene related information from genome flatfile
Input nucleotide sequence(s): refseqn:NC_000913
List of gene name(s) to report [*]: recA,recB
Name of gene feature to access: translation
Sequence output file [nc_000913.translation.genret]: stdout
>recA
MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDIALGAGGLPMGR
IVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDT
GEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEIGDSHMGLAARMMSQAMRKLAGNL
KQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVGSETR
VKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKAN
ATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSVDDSEGVAETNEDF
>recB
MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGLGGSAAFPRPLTVEELLV
VTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLEEIDDKAQAAQWLLLAERQMD
EAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLRYQACADFWRRHCYPLPREIAQVV
FETWKGPQALLRDINRYLQGEAPVIKAPPPDDETLASRHAQIVARIDTVKQQWRDAVGELD
ALIESSGIDRRKFNRSNQAKWIDKISAWAEEETNSYQLPESLEKFSQRFLEDRTKAGGETP
RHPLFEAIDQLLAEPLSIRDLVITRALAEIRETVAREKRRRGELGFDDMLSRLDSALRSES
GEVLAAAIRTRFPVAMIDEFQDTDPQQYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADI
FTYMKARSEVHAHYTLDTNWRSAPGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALR
FVFKGETQPAMKMWLMEGESCGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPV
RASDISVLVRSRQEAAQVRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPEREN
TLRSALATSMMGLNALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIA
ENLLATAGGERRLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESD
KHLVQIVTIHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAE
RLAEDLRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLR
TCIEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGHG
IAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNWVREK
LELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEPLIASQLD
TLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGEDSSAYTQQAM
AAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYEHHFGGVIYLFLRGVDKEHPQQGIYTTR
PNAGLIALMDEMFAGMTLEEA
</pre></td></tr></table>
Go to the <a href="#input">input files</a> for this example<br>
Go to the <a href="#output">output files</a> for this example<br><br>
Example 3<br><br>
Using a file with a list of gene names.
The following example will retrieve the strand direction for each gene
listed in the "gene_list.txt" file. String prefixed with an "@" or "list::"
will be interpreted as file names.<br><br>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% genret
Retrieves various gene features from genome flatfile
Input nucleotide sequence(s): refseqn:NC_000913
List of gene name(s) to report [*]: @gene_list.txt
Name of gene feature to access: direction
Full text output file [nc_000913.direction]: stdout
gene,direction
thrA,direct
thrB,direct
thrC,direct
</pre></td></tr></table>
Example 4<br><br>
Retrieving translations of coding sequences.<br>
The following example will retrieve the translated protein sequence of
the "recA" gene.<br><br>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% genret
Retrieves various gene related information from genome flatfile
Input nucleotide sequence(s): refseqn:NC_000913
Gene name(s) to lookup [*]: recA
Feature to access: translation
Full text output file [nc_000913.translation]: stdout
>recA
MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDIALGAGGLPMGR
IVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDT
GEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEIGDSHMGLAARMMSQAMRKLAGNL
KQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVGSETR
VKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKAN
ATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSVDDSEGVAETNEDF
</pre></td></tr></table>
Go to the <a href="#input">input files</a> for this example<br>
Go to the <a href="#output">output files</a> for this example<br><br>
Example 5<br><br>
Retrieving feature information of the genes.<br>
The following example will retrieve the start positions for each gene.
The values for the keys in GenBank format is available for retrieval.
(ex. start end direction GO* etc.)<br>
Positions will be returned with a 1 start value.<br><br>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% genret
Retrieves various gene related information from genome flatfile
Input nucleotide sequence(s): refseqn:NC_000913
Gene name(s) to lookup [*]:
Feature to access: start
Full text output file [nc_000913.start]:
</pre></td></tr></table>
Go to the <a href="#input">input files</a> for this example<br>
Go to the <a href="#output">output files</a> for this example<br><br>
Example 6<br><br>
Passing extra arguments to the methods.<br>
The following example shows the retrieval of 30 base pairs around the
start codon of the "recA" gene. By default, the "around_startcodon" method
returns 200 base pairs around the start codon. Using the "-argument"
qualifier allows the user to change this value.<br><br>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% genret refseqn:NC_000913 recA around_startcodon -argument 30,30 stdout
Retrieves various gene features from genome flatfile
>recA
ccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgt
tg
</pre></td></tr></table>
Go to the <a href="#input">input files</a> for this example<br>
Go to the <a href="#output">output files</a> for this example<br><br>
Example 7<br><br>
Re-annotating a flatfile.
genret supports re-annotation of a genome flatfile via Restauro-G
service developed by our team.
The original software is available at [<a href="http://restauro-g.iab.keio.ac.jp/">http://restauro-g.iab.keio.ac.jp</a>].<br><br>
<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>
% genret refseqn:NC_000913 '*' annotate nc_000913-annotate.gbk
Retrieves various gene features from genome flatfile
</pre></td></tr></table>
Go to the <a href="#input">input files</a> for this example<br>
Go to the <a href="#output">output files</a> for this example<br><br>
<h2>Command line arguments</h2>
<table border cellspacing=0 cellpadding=3 bgcolor="#ccccff">
<tr bgcolor="#FFFFCC">
<th align="left">Qualifier</th>
<th align="left">Type</th>
<th align="left">Description</th>
<th align="left">Allowed values</th>
<th align="left">Default</th>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Standard (Mandatory) qualifiers</th>
</tr>
<tr bgcolor="#FFFFCC">
<td>[-sequence]<br>(Parameter 1)</td>
<td>seqall</td>
<td>Nucleotide sequence(s) filename and optional format, or reference (input USA)</td>
<td>Readable sequence(s)</td>
<td><b>Required</b></td>
</tr>
<tr bgcolor="#FFFFCC">
<td>[-gene]<br>(Parameter 2)</td>
<td>string</td>
<td>List of gene name(s) to report</td>
<td>Any string</td>
<td>*</td>
</tr>
<tr bgcolor="#FFFFCC">
<td>[-access]<br>(Parameter 3)</td>
<td>string</td>
<td>Name of gene feature to access</td>
<td>Any word</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td>[-outfile]<br>(Parameter 4)</td>
<td>outfile</td>
<td>Sequence output file</td>
<td>Output file</td>
<td><i><*></i>.genret</td>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Additional (Optional) qualifiers</th>
</tr>
<tr>
<td colspan=5>(none)</td>
</tr>
<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Advanced (Unprompted) qualifiers</th>
</tr>
<tr bgcolor="#FFFFCC">
<td>-argument</td>
<td>string</td>
<td>Extra arguments to pass to method</td>
<td>Any string</td>
<td> </td>
</tr>
<tr bgcolor="#FFFFCC">
<td>-[no]accid</td>
<td>boolean</td>
<td>Include to use sequence accession ID as query</td>
<td>Boolean value Yes/No</td>
<td>Yes</td>
</tr>
</table>
<h2 id="input">Input file format</h2>
<p>
Database definitions for the examples are included in the embossrc_template<br />
file of the Keio Bioinformatcs Web Service (KBWS) package.<br />
<br />
Input files for usage example 4<br />
<br />
File: gene_list.txt<br />
<br />
thrA<br />
thrB<br />
thrC<br />
<br />
</p>
<h2 id="output">Output file format</h2>
<p>
Output files for usage example 1<br />
<br />
File: nc_000913.around_startcodon<br />
<br />
<table width="90%"><tr><td bgcolor="#CCFFCC">
>thrL<br />
cgtgagtaaattaaaattttattgacttaggtcactaaatactttaaccaatataggcata<br />
gcgcacagacagataaaaattacagagtacacaacatccatgaaacgcattagcaccacca<br />
ttaccaccaccatcaccattaccacaggtaacggtgcgggctgacgcgtacaggaaacaca<br />
gaaaaaagcccgcacctgac<br />
>thrA<br />
aggtaacggtgcgggctgacgcgtacaggaaacacagaaaaaagcccgcacctgacagtgc<br />
gggctttttttttcgaccaaaggtaacgaggtaacaaccatgcgagtgttgaagttcggcg<br />
gtacatcagtggcaaatgcagaacgttttctgcgtgttgccgatattctggaaagcaatgc<br />
caggcaggggcaggtggcca<br />
<br />
<font color=red>[Part of this file has been deleted for brevity]</font><br />
<br />
>yjjY<br />
tgcatgtttgctacctaaattgccaactaaatcgaaacaggaagtacaaaagtccctgacc<br />
tgcctgatgcatgctgcaaattaacatgatcggcgtaacatgactaaagtacgtaattgcg<br />
ttcttgatgcactttccatcaacgtcaacaacatcattagcttggtcgtgggtactttccc<br />
tcaggacccgacagtgtcaa<br />
>yjtD<br />
tttttctgcgacttacgttaagaatttgtaaattcgcaccgcgtaataagttgacagtgat<br />
cacccggttcgcggttatttgatcaagaagagtggcaatatgcgtataacgattattctgg<br />
tcgcacccgccagagcagaaaatattggggcagcggcgcgggcaatgaaaacgatggggtt<br />
tagcgatctgcggattgtcg<br />
</td></tr></table>
<br />
Output files for usage example 3<br />
<br />
File: nc_000913.start<br />
<br />
<table width="90%"><tr><td bgcolor="#CCFFCC">
gene,start<br />
thrL,190<br />
thrA,337<br />
thrB,2801<br />
thrC,3734<br />
yaaX,5234<br />
yaaA,5683<br />
yaaJ,6529<br />
talB,8238<br />
mog,9306<br />
<br />
<font color=red>[Part of this file has been deleted for brevity]</font><br />
<br />
yjjX,4631256<br />
ytjC,4631820<br />
rob,4632464<br />
creA,4633544<br />
creB,4634030<br />
creC,4634719<br />
creD,4636201<br />
arcA,4637613<br />
yjjY,4638425<br />
yjtD,4638965<br />
</td></tr></table><br />
Output files for usage example 7<br />
<br />
File: ecoli-annotate.gbk<br />
<br />
<table width="90%"><tr><td bgcolor="#CCFFCC">
LOCUS NC_000913 4639675 bp DNA circular BCT 25-OCT-2010<br />
DEFINITION Escherichia coli str. K-12 substr. MG1655 chromosome, complete<br />
genome.<br />
ACCESSION NC_000913<br />
VERSION NC_000913.2 GI:49175990<br />
DBLINK Project: 57779<br />
KEYWORDS .<br />
SOURCE Escherichia coli str. K-12 substr. MG1655<br />
ORGANISM Escherichia coli str. K-12 substr. MG1655<br />
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;<br />
<br />
<font color="red">[Part of this file has been deleted for brevity]</font><br />
<br />
CDS 2801..3733<br />
/EC_number="2.7.1.39"<br />
/codon_start="1"<br />
/db_xref="GI:16127997"<br />
/db_xref="ASAP:ABE-0000010"<br />
/db_xref="UniProtKB/Swiss-Prot:P00547"<br />
/db_xref="ECOCYC:EG10999"<br />
/db_xref="EcoGene:EG10999"<br />
/db_xref="GeneID:947498"<br />
/function="enzyme; Amino acid biosynthesis: Threonine"<br />
/function="1.5.1.8 metabolism; building block<br />
biosynthesis; amino acids; threonine"<br />
/function="7.1 location of gene products; cytoplasm"<br />
/gene="thrB"<br />
/gene_synonym="ECK0003; JW0002"<br />
/locus_tag="b0003"<br />
/note="GO_component: GO:0005737 - cytoplasm; GO_process:<br />
GO:0009088 - threonine biosynthetic process"<br />
/product="homoserine kinase"<br />
/protein_id="NP_414544.1"<br />
/rs_com="FUNCTION: Catalyzes the ATP-dependent<br />
phosphorylation of L- homoserine to L-homoserine<br />
phosphate (By similarity)."<br />
/rs_com="CATALYTIC ACTIVITY: ATP + L-homoserine = ADP +<br />
O-phospho-L- homoserine."<br />
/rs_com="PATHWAY: Amino-acid biosynthesis; L-threonine<br />
biosynthesis; L- threonine from L-aspartate: step 4/5."<br />
/rs_com="SUBCELLULAR LOCATION: Cytoplasm (Potential)."<br />
/rs_com="SIMILARITY: Belongs to the GHMP kinase family.<br />
Homoserine kinase subfamily."<br />
/rs_des="RecName: Full=Homoserine kinase; Short=HK;<br />
Short=HSK; EC=2.7.1.39;"<br />
/rs_protein="Level 1: similar to KHSE_ECODH 1.7e-180"<br />
/rs_xr="EMBL; CP000948; ACB01208.1; -; Genomic_DNA."<br />
/rs_xr="RefSeq; YP_001728986.1; -."<br />
/rs_xr="ProteinModelPortal; B1XBC8; -."<br />
/rs_xr="SMR; B1XBC8; 2-308."<br />
/rs_xr="EnsemblBacteria; EBESCT00000012034;<br />
EBESCP00000011562; EBESCG00000011096."<br />
/rs_xr="GeneID; 6058639; -."<br />
/rs_xr="GenomeReviews; CP000948_GR; ECDH10B_0003."<br />
/rs_xr="KEGG; ecd:ECDH10B_0003; -."<br />
/rs_xr="HOGENOM; HBG646290; -."<br />
/rs_xr="OMA; GSAHADN; -."<br />
/rs_xr="ProtClustDB; PRK01212; -."<br />
/rs_xr="BioCyc; ECOL316385:ECDH10B_0003-MONOMER; -."<br />
/rs_xr="GO; GO:0005737; C:cytoplasm;<br />
IEA:UniProtKB-SubCell."<br />
/rs_xr="GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW."<br />
/rs_xr="GO; GO:0004413; F:homoserine kinase activity;<br />
IEA:EC."<br />
/rs_xr="GO; GO:0009088; P:threonine biosynthetic process;<br />
IEA:UniProtKB-KW."<br />
/rs_xr="HAMAP; MF_00384; Homoser_kinase; 1; -."<br />
/rs_xr="InterPro; IPR006204; GHMP_kinase."<br />
/rs_xr="InterPro; IPR013750; GHMP_kinase_C."<br />
/rs_xr="InterPro; IPR006203; GHMP_knse_ATP-bd_CS."<br />
/rs_xr="InterPro; IPR000870; Homoserine_kin."<br />
/rs_xr="InterPro; IPR020568; Ribosomal_S5_D2-typ_fold."<br />
/rs_xr="InterPro; IPR014721;<br />
Ribosomal_S5_D2-typ_fold_subgr."<br />
/rs_xr="Gene3D; G3DSA:3.30.230.10;<br />
Ribosomal_S5_D2-type_fold; 1."<br />
/rs_xr="Pfam; PF08544; GHMP_kinases_C; 1."<br />
/rs_xr="Pfam; PF00288; GHMP_kinases_N; 1."<br />
/rs_xr="PIRSF; PIRSF000676; Homoser_kin; 1."<br />
/rs_xr="PRINTS; PR00958; HOMSERKINASE."<br />
/rs_xr="SUPFAM; SSF54211; Ribosomal_S5_D2-typ_fold; 1."<br />
/rs_xr="TIGRFAMs; TIGR00191; thrB; 1."<br />
/rs_xr="PROSITE; PS00627; GHMP_KINASES_ATP; 1."<br />
/transl_table="11"<br />
/translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF<br />
SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS<br />
VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI<br />
ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ<br />
PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPETA<br />
QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"<br />
<br />
<font color="red">[Part of this file has been deleted for brevity]</font><br />
<br />
4639201 gcgcagtcgg gcgaaatatc attactacgc cacgccagtt gaactggtgc cgctgttaga<br />
4639261 ggaaaaatct tcatggatga gccatgccgc gctggtgttt ggtcgcgaag attccgggtt<br />
4639321 gactaacgaa gagttagcgt tggctgacgt tcttactggt gtgccgatgg tggcggatta<br />
4639381 tccttcgctc aatctggggc aggcggtgat ggtctattgc tatcaattag caacattaat<br />
4639441 acaacaaccg gcgaaaagtg atgcaacggc agaccaacat caactgcaag ctttacgcga<br />
4639501 acgagccatg acattgctga cgactctggc agtggcagat gacataaaac tggtcgactg<br />
4639561 gttacaacaa cgcctggggc ttttagagca acgagacacg gcaatgttgc accgtttgct<br />
4639621 gcatgatatt gaaaaaaata tcaccaaata aaaaacgcct tagtaagtat ttttc<br />
//<br />
</td></tr></table>
</p>
<h2>Data files</h2>
<p>
None.
</p>
<h2>Notes</h2>
<p>
None.
</p>
<h2>References</h2>
<pre>
Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and
Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench
for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306.
Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for
large-scale analysis of high-throughput omics data, J. Pest Sci.,
31, 7.
Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome
Analysis Environment with REST and SOAP Web Service Interfaces,
Nucleic Acids Res., 38, W700-W705.
</pre>
<h2>Warnings</h2>
<p>
None.
</p>
<h2>Diagnostic Error Messages</h2>
<p>
None.
</p>
<h2>Exit status</h2>
<p>
It always exits with a status of 0.
</p>
<h2>Known bugs</h2>
<p>
None.
</p>
<h2>See also</h2>
<table border cellpadding=4 bgcolor="#FFFFF0"><tr><th>Program name</th>
<th>Description</th></tr>
<tr>
<td><a href="entret">entret</a></td>
<td>Retrieve sequence entries from flatfile databases and files</td>
</tr><tr>
<td><a href="seqret">seqret</a></td>
<td>Read and write (return) sequences</td>
</tr>
</table>
<h2>Author(s)</h2>
<pre>
Hidetoshi Itaya (celery@g-language.org)
Institute for Advanced Biosciences, Keio University
252-0882 Japan
Kazuharu Arakawa (gaou@sfc.keio.ac.jp)
Institute for Advanced Biosciences, Keio University
252-0882 Japan</pre>
<h2>History</h2>
2012 - Written by Hidetoshi Itaya
<h2>Target users</h2>
This program is intended to be used by everyone and everything, from
naive users to embedded scrips.
<h2>Comments</h2>
None.
</HTML>
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