Mercurial > repos > kuyt002 > mummer_toolsuite
diff MUMmer/mummer_tool.xml @ 0:61f30d177448 default tip
initial commit on Mummer toolsuite on toolshed
author | eric |
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date | Tue, 31 Mar 2015 14:19:49 +0200 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/MUMmer/mummer_tool.xml Tue Mar 31 14:19:49 2015 +0200 @@ -0,0 +1,114 @@ +<tool id="mummer_tool" name="MUMmer compare and plot" version="0.4.alx" force_history_refresh="True"> + <description>: Compare and plot genomes (Nucmer or Promer)</description> + <command interpreter="bash"> + mummer_tool.sh + $input_ref $input_query + $out_delta $out_coords $out_png $out_log + $algorithm + $keep_delta $make_coords $keep_log $make_image + $cmd_extra + </command> + <inputs> + <param name="algorithm" type="select" format="text" value="nucmer" label="Algorithm" help="Nucmer dna or Promer protein (FASTA: protein. Dna is six frame translated)"> + <option value="nucmer">Nucmer DNA</option> + <option value="promer">Promer</option> + </param> + <param name="input_ref" type="data" format="fasta" label="Reference sequence" /> + <param name="input_query" type="data" format="fasta" label="Sequence query file"/> + <param name="make_image" type="select" format="text" value="yes" label="Generate MUMmerplot" help="MUMmerplot will be run with default settings and --large --png as fixed image."> + <option value="yes">Yes</option> + <option value="no">No</option> + </param> + <param name="keep_delta" type="select" format="text" value="no" label="Keep delta file" help="i.e. for further processing"> + <option value="no">No</option> + <option value="yes">Yes</option> + </param> + <param name="make_coords" type="select" format="text" value="yes" label="Make coords file" help="Uses the -r argument to sort lines by reference."> + <option value="no">No</option> + <option value="yes">Yes</option> + </param> + <param name="keep_log" type="select" format="text" value="no" label="Keep console log file" help="i.e. for debugging"> + <option value="no">No</option> + <option value="yes">Yes</option> + </param> + <param name="cmd_extra" type="text" size="40" value="" label="Extra cmd line options" help="the --coords is run by default" /> + </inputs> + <outputs> + <data name="out_coords" format="tabular" label="${algorithm.value_label} coords"> + <filter>make_coords=="yes"</filter> + </data> + <data name="out_delta" format="tabular" label="${algorithm.value_label} delta"> + <filter>keep_delta=="yes"</filter> + </data> + <data name="out_png" format="png" label="${algorithm.value_label} mummerplot"> + <filter>make_image=="yes"</filter> + </data> + <data name="out_log" format="tabular" label="Console log file"> + <filter>keep_log=="yes"</filter> + </data> + </outputs> + <requirements> + <requirement type="package" version="4.6">gnuplot</requirement> + <requirement type="package" version="3.23">mummer</requirement> + </requirements> + <tests> + <test> + <param name="algorithm" value="nucmer" /> + <param name="input_ref" value="test.seq1.fasta"/> + <param name="input_query" value="test.seq2.fasta"/> + <param name="make_image" value="yes"/> + <param name="keep_delta" value="no" /> + <param name="make_coords" value="no" /> + <param name="keep_log" value="yes" /> + + <output name="out_log" file="test.MUMmerplot.result.Nucmer_galaxy.log" /> + <output name="out_png" file="test.MUMmerplot.result.Nucmer_galaxy.png" /> + </test> + </tests> + <help> +| + + +**Reference** +------------- + +- **Nucmer Galaxy tool wrapper: Alex Bossers, CVI of Wageningen UR, The Netherlands.** + +- **Nucmer or Promer of MUMmer suite:** v3.22 http://mummer.sourceforge.net/manual/ + +- **MUMmer tutorials:** http://mummer.sourceforge.net/examples/ + + +If you found these tools/wrappers useful in your research, please acknowledge our work. If you improve +or modify the wrappers please add instead of substitute yourself into the acknowlegement section :) + + +**Command line arguments** +-------------------------- + +--mum Use anchor matches that are unique in both the reference and query +--mumreference Use anchor matches that are unique in the reference but not necessarily unique in the query (default behavior) +--maxmatch Use all anchor matches regardless of their uniqueness +--breaklen Distance an alignment extension will attempt to extend poor scoring regions before giving up (default 200) +--mincluster Minimum cluster length (default 65) +--delta Toggle the creation of the delta file. Setting --nodelta prevents the alignment extension step and only outputs the match clusters (default --delta) +--depend Print the dependency information and exit +--diagfactor Maximum diagonal difference factor for clustering, i.e. diagonal difference / match separation (default 0.12) +--extend Toggle the outward extension of alignments from their anchoring clusters. Setting --noextend will prevent alignment extensions but still align the DNA between clustered matches and create the .delta file (default --extend) +--forward Align only the forward strands of each sequence +--maxgap Maximum gap between two adjacent matches in a cluster (default 90) +--help Print the help information and exit +--minmatch Minimum length of an maximal exact match (default 20) +--optimize Toggle alignment score optimization. Setting --nooptimize will prevent alignment score optimization and result in sometimes longer, but lower scoring alignments (default --optimize) +--reverse Align only the reverse strand of the query sequence to the forward strand of the reference +--simplify Simplify alignments by removing shadowed clusters. Turn this option off (--nosimplify) if aligning a sequence to itself to look for repeats (default --simplify) +--version Print the version information and exit +--coords **Automatically ON in galaxy wrapper!** It generates the .coords file using the 'show-coords' program with the -r option. +--prefix **Do NOT use in Galaxy wrapper!** Set the output file prefix (default out) + +| +| + + </help> +</tool> +