Mercurial > repos > kyost > atac_primer_tool
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author | kyost |
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date | Sat, 28 Apr 2018 15:07:26 -0400 |
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<tool id="window_coverage" name="Calculate window coverage" version="0.1.0"> <description> Determines spanning and total read coverage for overlapping windows spanning peaks of interest. </description> <requirements> <requirement type="package" version="1.3">samtools</requirement> <requirement type="package" version="2.27.0.0">bedtools</requirement> </requirements> <stdio> <exit_code range="1:" /> </stdio> <command><![CDATA[ #set input_list = '","'.join( [str( $input.name ) for $input in $input1 ] ) $__tool_directory__/./window_coverage.bash "$input1" "$input_list" "$input2" "$input3" "$output1" "$output2" ]]></command> <inputs> <param type="data" name="input1" format="bam" multiple="true" label="Filtered Bam Files"/> <param type="data" name="input2" format="bed" label="qPCR Window Bed File"/> <param type="float" name="input3" value="0.9" label="Coverage Cutoff"/> </inputs> <outputs> <data name="output1" format="bed" label="Coverage Bed File"/> <data name="output2" format="bed" label="Spanning Fragment Coverage Bed File" /> </outputs> <tests> <test> <param name="input1" value="Ch1-1.pe.q10.sort.bam.rmdup.filter.bam,Ch13-1.pe.q10.sort.rmdup.filter.bam"/> <param name="input2" value="qPCR_windows.bed" /> <param name="input3" value="0.9" /> <output name="output1" file="combined.o.coverage"/> <output name="output2" file="combined.f9.coverage"/> </test> </tests> <help><![CDATA[ This tools takes a series of bam files and a bed file of overlapping windows spanning peaks of interest and determines the read coverage and spanning fragment coverage for each window. ]]></help> <citations> <citation type="doi">doi:10.1038/nmeth.4663</citation> </citations> </tool>