Mercurial > repos > ldelisle > revertr2orientationinbam
view revertR2orientationInBam.sh @ 1:2c86ad672f52 draft default tip
planemo upload commit 89aa8abdc88aa922cab8d9c5f17078ba1e388339-dirty
author | ldelisle |
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date | Wed, 19 Oct 2022 08:34:43 +0000 |
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#!/bin/bash inputBam=$1 outputBam=$2 samtools view -h "$inputBam" | awk -v OFS="\t" '{ # Process only non header lines if(!($1~/^@/)){ # SAM flag is field 2 n=$2 # Change only the second in pair flag is 128 d=128 q=(n-n%d)/d+(n<0) if(q%2==1){ # Evaluate the strand reverse strand flag is 16 d=16 q=(n-n%d)/d+(n<0) if(q%2==1){ # It is reverse it is now forward $2-=16 }else{ # It is forward it is now reverse $2+=16 } } } print }' | samtools view -b - > "$outputBam"