changeset 21:b575af79e250 draft

Uploaded
author martasampaio
date Sat, 20 Apr 2019 11:06:06 -0400
parents b680802b13cc
children 5acc4fa8b62d
files README.rst
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+===============
+PhagePromoter
+===============
+
+Get promoter of phage genomes
+
+PhagePromoter is a python script that predicts promoter sequences in phage genomes, using a machine learning SVM model. This model was built from a train dataset with 19 features and 3200 examples (800 positives and 2400 negatives), each representing a 65 bp sequence of a phage genome. The positive cases represent the phage sequences that are already identified as promoters. 
+
+**Inputs:**
+
+* genome format: fasta vs genbank; 
+* genome file: acepts both genbank and fasta formats;
+* both strands (yes or no): allows the search in both DNA strands;
+* threshold: represents the probability of the test sequence be a promoter (float between 0 and 1)"
+* family: The family of the testing phage - Podoviridae, Siphoviridae or Myoviridae;
+* Bacteria: The host of the phage. The train dataset include the following hosts: Bacillus, EColi, Salmonella, Pseudomonas, Yersinia, Klebsiella, Pectobacterium, Morganella, Cronobacter, Staphylococcus, Streptococcus, Streptomyces, Lactococcus. If the testing phage has a different host, select the option 'other', and it is recommended the use of a higher threshold value for more accurate results.
+* phage type: The type of the phage, according to its lifecycle: virulent or temperate;
+
+**Outputs:**
+This tool outputs two files: a FASTA file and a table in HTML, with the locations, sequence, score and type (recognized by host or phage RNAP) of the predicted promoters.
+
+**Requirements:**
+
+* Biopython
+* Sklearn 
+* Numpy
+* Pandas 
+
+