40
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1 #echo "1: " $1" 2: " $2" 3: " $3" 4: "$4" 5: "$5 " 6: "$6 "7: "$7" 8: "$8 " 9: "$9" 10: "${10}" 11: "${11} "12: "${12}
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2 #echo " 13: "${13}" 14: "${14}" 15: "${15}" 16: "${16} "17: "${17}" 18: "${18}" 19: "${19}" 20: "${20}" 21: "${21} " 22: "${22}" 23:" ${23}
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3
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4 #Count total number of parameters and classification parameters
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5 parmSize=0
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6 classSize=0
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7 matrixSize=0
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8 for i in "$@"; do
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9 currParm=$(cut -d'|' -f1 <<< $i)
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10 parmSize=$((parmSize+1))
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11 if [ $currParm = "classification" ]
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12 then
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13 classSize=$((classSize+1))
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14 fi
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15 done
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16
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17 #Get tool data and tool install directories
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18 tooldir=$1
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19 tooldata=$2
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20 #create temp directory for row and col order and dendro files.
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21 tdir=$tooldata/$(date +%y%m%d%M%S)
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22 mkdir $tdir
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23 #echo "tdir: "$tdir
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24
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25 #Extract parameters for row and column order and dendro files
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26 rowOrderFile=$tdir/ROfile.txt
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27 rowDendroFile=$tdir/RDfile.txt
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28 colOrderFile=$tdir/COfile.txt
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29 colDendroFile=$tdir/CDfile.txt
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30 rowOrderJson='"order_file": "'$rowOrderFile'",'
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31 rowDendroJson='"dendro_file": "'$rowDendroFile'",'
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32 colOrderJson='"order_file": "'$colOrderFile'",'
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33 colDendroJson='"dendro_file": "'$colDendroFile'",'
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34
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35 #BEGIN: Construct JSON for all non-repeating parameters
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36 parmJson='{'
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37 rowConfigJson='"row_configuration": {'
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38 colConfigJson='"col_configuration": {'
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39
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40 ctr=0
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41 for i in "$@"; do
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42 if [ $ctr -gt 1 ]
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43 then
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44 currParm=$(cut -d'|' -f1 <<< $i)
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45 if [ $currParm != "matrix_files" ] && [ $currParm != "row_configuration" ] && [ $currParm != "col_configuration" ] && [ $currParm != "classification" ]
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46 then
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47 #Parse pipe-delimited parameter parameter
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48 parmJson=$parmJson' "'$(cut -d'|' -f1 <<< $i)'":"'$(cut -d'|' -f2 <<< $i)'",'
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49 fi
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50 if [ $currParm = "row_configuration" ]
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51 then
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52 rowOrder=$(cut -d'|' -f3 <<< $i)
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53 rowDistance=$(cut -d'|' -f5 <<< $i)
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54 rowAgglomeration=$(cut -d'|' -f7 <<< $i)
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55 rowCuts=$(cut -d'|' -f9 <<< $i)
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56 rowLabels=$(cut -d'|' -f11 <<< $i)
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57 dataTypeJson='"'$(cut -d'|' -f10 <<< $i)'":["'$rowLabels'"]'
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58 if [ $rowOrder = 'Hierarchical' ]
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59 then
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60 rowConfigJson=$rowConfigJson$rowOrderJson$rowDendroJson
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61 fi
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62 rowConfigJson=$rowConfigJson'"'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'","'$(cut -d'|' -f6 <<< $i)'":"'$(cut -d'|' -f7 <<< $i)'",'$dataTypeJson'},'
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63 fi
|
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64 if [ $currParm = "col_configuration" ]
|
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65 then
|
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66 colOrder=$(cut -d'|' -f3 <<< $i)
|
|
67 colDistance=$(cut -d'|' -f5 <<< $i)
|
|
68 colAgglomeration=$(cut -d'|' -f7 <<< $i)
|
|
69 colCuts=$(cut -d'|' -f9 <<< $i)
|
|
70 colLabels=$(cut -d'|' -f11 <<< $i)
|
|
71 dataTypeJson='"'$(cut -d'|' -f10 <<< $i)'":["'$colLabels'"]'
|
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72 if [ $colOrder = 'Hierarchical' ]
|
|
73 then
|
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74 colConfigJson=$colConfigJson$colOrderJson$colDendroJson
|
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75 fi
|
|
76 colConfigJson=$colConfigJson'"'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'","'$(cut -d'|' -f6 <<< $i)'":"'$(cut -d'|' -f7 <<< $i)'",'$dataTypeJson'},'
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|
77 fi
|
|
78 fi
|
|
79 ctr=$((ctr+1))
|
|
80 done
|
|
81 #END: Construct JSON for all non-repeating parameters
|
|
82 #echo "rowCuts: "$rowCuts
|
|
83 #echo "colCuts: "$colCuts
|
|
84 #echo "ROW CONFIG JSON: "$rowConfigJson
|
|
85 #echo "COL CONFIG JSON: "$colConfigJson
|
|
86
|
|
87 #BEGIN: Construct JSON for data layers
|
|
88 matrixJson='"matrix_files": [ '
|
|
89 inputMatrix=''
|
|
90 for i in "$@"; do
|
|
91 currParm=$(cut -d'|' -f1 <<< $i)
|
|
92 if [ $currParm = "matrix_files" ]
|
|
93 then
|
|
94 #Parse pipe-delimited parameter parameter
|
|
95 matrixJson=$matrixJson' {"'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'","'$(cut -d'|' -f6 <<< $i)'":"'$(cut -d'|' -f7 <<< $i)'"}'
|
|
96 inputMatrix=$(cut -d'|' -f3 <<< $i)
|
|
97 fi
|
|
98 done
|
|
99 matrixJson=$matrixJson"],"
|
|
100 #END: Construct JSON for data layers
|
|
101
|
|
102 #BEGIN: Construct JSON for classification files
|
|
103 classJson='"classification_files": [ '
|
|
104 classIter=0
|
|
105 for i in "$@"; do
|
|
106 currParm=$(cut -d'|' -f1 <<< $i)
|
|
107 if [ $currParm = "classification" ]
|
|
108 then
|
|
109 classIter=$((classIter+1))
|
|
110 #Parse pipe-delimited 3-part classification bar parameter
|
|
111 classJson=$classJson' {"'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'"'
|
|
112 classCat=$(cut -d'|' -f7 <<< $i)
|
|
113 classColorType=$(cut -d'_' -f2 <<< $classCat)
|
|
114 classJson=$classJson','
|
|
115 classJson=$classJson' "position":"'$(cut -d'_' -f1 <<< $classCat)'","color_map": {"type":"'$classColorType'"}}'
|
|
116 if [ $classIter -lt $classSize ]
|
|
117 then
|
|
118 classJson=$classJson','
|
|
119 fi
|
|
120 fi
|
|
121 done
|
|
122 classJson=$classJson']'
|
|
123 #END: Construct JSON for classification files
|
|
124
|
|
125 parmJson=$parmJson$matrixJson$rowConfigJson$colConfigJson$classJson
|
|
126 parmJson=$parmJson'}'
|
|
127 #echo "HEATMAP PARAMETERS JSON: "$parmJson
|
|
128
|
|
129 #run R to cluster matrix
|
|
130 output="$(R --slave --vanilla --file=$tooldir/CHM.R --args $inputMatrix $rowOrder $rowDistance $rowAgglomeration $colOrder $colDistance $colAgglomeration $rowOrderFile $colOrderFile $rowDendroFile $colDendroFile $rowCuts $colCuts $rowLabels $colLabels 2>&1)"
|
|
131 rc=$?;
|
|
132 if [ $rc != 0 ]
|
|
133 then
|
|
134 echo $output;
|
|
135 if [ `echo "$output" | grep -c "Inf in foreign function call"` -gt 0 ]
|
|
136 then
|
|
137 echo "";
|
|
138 echo "Note: This error can occur when there is no variation in a row or column. Try a different distance measure or remove rows/columns without variation.";
|
|
139 echo "This error may also be caused when a covariate file has inadvertently been selected as an Input Matrix. Check your Input Matrix entry.";
|
|
140 fi
|
|
141 exit $rc;
|
|
142 fi
|
|
143
|
|
144 #call java program to generate NGCHM viewer files.
|
|
145 java -jar $tooldir/GalaxyMapGen.jar "$parmJson"
|
|
146 #clean up tempdir
|
|
147 rm -rf $tdir
|