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1 <tool id="gd_specify_restriction_enzymes" name="Specify" version="1.0.0">
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2 <description>a set of restriction enzymes</description>
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3
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4 <command interpreter="python">
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5 specify_restriction_enzymes.py "--input=$input" "--output=$output" "--primers_loc=${GALAXY_DATA_INDEX_DIR}/gd.primers.loc"
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6 #if $override_metadata.choice == "0":
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7 "--scaffold_col=${input.metadata.scaffold}" "--pos_col=${input.metadata.pos}" "--species=${input.metadata.species}"
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8 #else
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9 "--scaffold_col=$scaf_col" "--pos_col=$pos_col" "--species=$species"
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10 #end if
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11 "--enzyme_list=$enzymes"
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12 </command>
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13
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14 <inputs>
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15 <param format="tabular" name="input" type="data" label="Selected SNPS dataset"/>
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16 <conditional name="override_metadata">
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17 <param name="choice" type="select" format="integer" label="choose columns">
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18 <option value="0" selected="true">No, get columns from metadata</option>
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19 <option value="1" >Yes, choose columns</option>
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20 </param>
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21 <when value="0" />
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22 <when value="1">
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23 <param name="scaf_col" type="data_column" data_ref="input" numerical="false" label="Column with scaffold"/>
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24 <param name="pos_col" type="data_column" data_ref="input" numerical="true" label="Column with position"/>
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25 <param name="species" type="select" label="Choose species">
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26 <options from_file="gd.species.txt">
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27 <column name="name" index="1"/>
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28 <column name="value" index="0"/>
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29 </options>
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30 </param>
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31 </when>
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32 </conditional>
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33
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34 <param name="enzymes" type="select" display="checkboxes" multiple="true" label="Choose enzymes">
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35 <options from_file="gd.restriction_enzymes.txt">
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36 <column name="name" index="0"/>
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37 <column name="value" index="1"/>
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38 </options>
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39 </param>
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40 </inputs>
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41
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42 <outputs>
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43 <data format="wsf" name="output" metadata_source="input"/>
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44 </outputs>
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45
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46 <tests>
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47 <test>
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48 <param name="input" value="test_out/select_snps/select_snps.wsf" ftype="wsf" />
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49 <param name="choice" value="0" />
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50 <param name="enzymes" value="Bsp1286I,HaeII,RsaI" />
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51 <output name="output" file="test_out/specify_restriction_enzymes/specify_restriction_enzymes.wsf" />
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52 </test>
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53 </tests>
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54
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55 <help>
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56 **What it does**
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57
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58 It selects the SNPs that are differentially cut by at least one of the
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59 specified restriction enzymes. The enzymes are required to cut the amplified
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60 segment (for the specified PCR primers) only at the SNP.
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61
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62 -----
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63
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64 **Example**
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65
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66 - input file::
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67
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68 chr2_75111355_75112576 314 A C L F chr2 75111676 C F 15 4 53 2 9 48 Y 96 0.369 0.355 0.396 0
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69 chr8_93901796_93905612 2471 A C A A chr8 93904264 A A 8 0 51 10 2 14 Y 961 0.016 0.534 0.114 2
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70 chr10_7434473_7435447 524 T C S S chr10 7435005 T S 11 5 90 14 0 69 Y 626 0.066 0.406 0.727 0
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71 chr14_80021455_80022064 138 G A H H chr14 80021593 G H 14 0 69 9 6 124 Y 377 0.118 0.997 0.195 1
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72 chr15_64470252_64471048 89 G A Y Y chr15 64470341 G Y 5 6 109 14 0 69 Y 312 0.247 0.998 0.393 0
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73 chr18_48070585_48071386 514 C T E K chr18 48071100 T K 7 7 46 14 0 69 Y 2 0.200 0.032 0.163 0
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74 chr18_50154905_50155664 304 A G Y C chr18 50155208 A Y 4 2 17 5 1 22 Y 8 0.022 0.996 0.128 0
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75 chr18_57379354_57380496 315 C T V V chr18 57379669 G V 11 0 60 9 6 62 Y 726 0.118 0.048 0.014 1
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76 chr19_14240610_14242055 232 C T A V chr19 14240840 C A 18 8 56 15 5 42 Y 73 0.003 0.153 0.835 0
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77 chr19_39866997_39874915 3117 C T P P chr19 39870110 C P 3 7 65 14 2 32 Y 6 0.321 0.911 0.462 4
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78 etc.
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79
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80 - output file::
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81
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82 chr8_93901796_93905612 2471 A C A A chr8 93904264 A A 8 0 51 10 2 14 Y 961 0.016 0.534 0.114 2
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83 chr14_80021455_80022064 138 G A H H chr14 80021593 G H 14 0 69 9 6 124 Y 377 0.118 0.997 0.195 1
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84 chr18_57379354_57380496 315 C T V V chr18 57379669 G V 11 0 60 9 6 62 Y 726 0.118 0.048 0.014 1
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85 chr19_39866997_39874915 3117 C T P P chr19 39870110 C P 3 7 65 14 2 32 Y 6 0.321 0.911 0.462 4
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86 etc.
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87 </help>
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88 </tool>
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