Mercurial > repos > mingchen0919 > aurora_deseq2_site
annotate 03_count_matrix_heatmap.Rmd @ 1:32210899a3dd draft
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author | mingchen0919 |
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date | Sun, 30 Dec 2018 12:45:56 -0500 |
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1 --- |
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2 title: 'Heatmap of count matrix' |
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3 output: |
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4 html_document: |
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5 highlight: pygments |
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6 --- |
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7 |
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8 ```{r setup, include=FALSE, warning=FALSE, message=FALSE} |
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9 knitr::opts_chunk$set(error = TRUE, echo = FALSE) |
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10 ``` |
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11 |
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12 |
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13 ```{r} |
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14 ntd <- normTransform(dds) |
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15 select <- order(rowMeans(counts(dds,normalized=TRUE)), |
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16 decreasing=TRUE)[1:20] |
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17 df <- as.data.frame(colData(dds)[, -ncol(colData(dds))]) |
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18 pheatmap(assay(ntd)[select,], annotation_col=df) |
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19 ``` |