Mercurial > repos > mingchen0919 > aurora_deseq2_site
annotate 03_count_matrix_heatmap.Rmd @ 2:ab8d558ea6e6 draft default tip
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
author | mingchen0919 |
---|---|
date | Sun, 30 Dec 2018 13:57:07 -0500 |
parents | 32210899a3dd |
children |
rev | line source |
---|---|
1
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
1 --- |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
2 title: 'Heatmap of count matrix' |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
3 output: |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
4 html_document: |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
5 highlight: pygments |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
6 --- |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
7 |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
8 ```{r setup, include=FALSE, warning=FALSE, message=FALSE} |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
9 knitr::opts_chunk$set(error = TRUE, echo = FALSE) |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
10 ``` |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
11 |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
12 |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
13 ```{r} |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
14 ntd <- normTransform(dds) |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
15 select <- order(rowMeans(counts(dds,normalized=TRUE)), |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
16 decreasing=TRUE)[1:20] |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
17 df <- as.data.frame(colData(dds)[, -ncol(colData(dds))]) |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
18 pheatmap(assay(ntd)[select,], annotation_col=df) |
32210899a3dd
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
mingchen0919
parents:
diff
changeset
|
19 ``` |