---
output: html_document
---

```{r setup, include=FALSE, warning=FALSE, message=FALSE}
knitr::opts_knit$set(progress = FALSE)
knitr::opts_chunk$set(error = TRUE, echo = FALSE)
```

### Per base N content

```{r 'Per base N content', fig.width=10}
## reads 1
pbNc_1 = extract_data_module(paste0(opt$X_d, '/read_1_fastqc/fastqc_data.txt'), 'Per base N content')
pbNc_1$id = 1:length(pbNc_1$X.Base)
pbNc_1$trim = 'before'

## reads 2
pbNc_2 = extract_data_module(paste0(opt$X_d, '/read_2_fastqc/fastqc_data.txt'), 'Per base N content')
pbNc_2$id = 1:length(pbNc_2$X.Base)
pbNc_2$trim = 'after'

comb_pbNc = rbind(pbNc_1, pbNc_2)
comb_pbNc$trim = factor(levels = c('before', 'after'), comb_pbNc$trim)

p = ggplot(data = comb_pbNc, aes(x = id, y = N.Count)) +
  geom_line(color = 'red') +
  scale_x_continuous(breaks = pbNc_2$id, labels = pbNc_2$X.Base) + 
  facet_grid(. ~ trim) +
  ylim(0, 1) + 
  xlab('N-Count') +
  ylab('') + 
  theme(axis.text.x = element_text(size = 5),
        axis.line = element_line(),
        panel.background = element_rect(fill = NA))
ggplotly(p)
```