Mercurial > repos > mingchen0919 > aurora_fastqc_site
view 10_adapter_content.Rmd @ 2:c64267b9f754 draft default tip
planemo upload commit 841d8b22bf9f1aaed6bfe8344b60617f45b275b2-dirty
author | mingchen0919 |
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date | Sun, 30 Dec 2018 12:48:14 -0500 |
parents | b7c115edd970 |
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--- output: html_document --- ```{r setup, include=FALSE, warning=FALSE, message=FALSE} knitr::opts_knit$set(progress = FALSE) knitr::opts_chunk$set(error = TRUE, echo = FALSE) ``` ### Adapter Content ```{r 'Adapter Content', fig.width=10} ## reads 1 ac_1 = extract_data_module(paste0(opt$X_d, '/read_1_fastqc/fastqc_data.txt'), 'Adapter Content') ac_1$id = 1:length(ac_1$X.Position) melt_ac_1 = melt(ac_1, id=c('X.Position', 'id')) melt_ac_1$trim = 'before' ## reads 2 ac_2 = extract_data_module(paste0(opt$X_d, '/read_2_fastqc/fastqc_data.txt'), 'Adapter Content') ac_2$id = 1:length(ac_2$X.Position) melt_ac_2 = melt(ac_2, id=c('X.Position', 'id')) melt_ac_2$trim = 'after' comb_ac = rbind(melt_ac_1, melt_ac_2) comb_ac$trim = factor(levels = c('before', 'after'), comb_ac$trim) p = ggplot(data = comb_ac, aes(x = id, y = value, color = variable)) + geom_line() + facet_grid(. ~ trim) + xlim(min(comb_ac$id), max(comb_ac$id)) + ylim(0, 1) + xlab('Position in read (bp)') + ylab('') + scale_color_discrete(name = '') + theme(axis.text.x = element_text(size = 5), panel.background = element_rect(fill = NA), axis.line = element_line()) ggplotly(p) ```