Mercurial > repos > mvdbeek > sra_tools
comparison fastq_dump.xml @ 0:9f74a22d2060 draft default tip
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/sra-tools commit 70fadb7e8972b1db550d0e067584930ce1ec8673-dirty
author | mvdbeek |
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date | Wed, 04 Nov 2015 06:57:32 -0500 |
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-1:000000000000 | 0:9f74a22d2060 |
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1 <tool id="fastq_dump" name="Extract reads" version="1.2.4"> | |
2 <description>in FASTQ/A format from NCBI SRA.</description> | |
3 <macros> | |
4 <import>sra_macros.xml</import> | |
5 </macros> | |
6 <expand macro="requirements"/> | |
7 <command> | |
8 <![CDATA[ | |
9 ## Need to set the home directory to the current working directory, | |
10 ## else the tool tries to write to home/.ncbi and fails when used | |
11 ## with a cluster manager. | |
12 export HOME=\$PWD; | |
13 vdb-config --restore-defaults; | |
14 #if $input.input_select == "file": | |
15 fastq-dump --log-level fatal --accession '${input.file.name}' | |
16 #else: | |
17 vdb-config -s "/repository/user/main/public/root=\$PWD"; | |
18 ## Do not use prefetch if region is specified, to avoid downloading | |
19 ## the complete sra file. | |
20 #if ( str( $adv.region ) == "" ) and ( str( $adv.minID ) == "" ) and ( str( $adv.maxID ) == "" ): | |
21 ASCP_PATH=`which ascp`; | |
22 ASCP_KEY=`dirname \$ASCP_PATH`/asperaweb_id_dsa.openssh; | |
23 prefetch --ascp-path "\$ASCP_PATH|\$ASCP_KEY" $input.accession; | |
24 #end if | |
25 ## Duplicate vdb-config, in case settings changed between prefetch and | |
26 ## dump command. | |
27 vdb-config -s "/repository/user/main/public/root=\$PWD"; | |
28 fastq-dump --accession "$input.accession" | |
29 #end if | |
30 --defline-seq '@\$sn[_\$rn]/\$ri' | |
31 --stdout | |
32 #if str( $adv.split ) == "yes": | |
33 --split-spot | |
34 #end if | |
35 #if str( $adv.alignments ) == "aligned": | |
36 --aligned | |
37 #end if | |
38 #if str( $adv.alignments ) == "unaligned": | |
39 --unaligned | |
40 #end if | |
41 #if str( $adv.minID ) != "": | |
42 --minSpotId "$adv.minID" | |
43 #end if | |
44 #if str( $adv.maxID ) != "": | |
45 --maxSpotId "$adv.maxID" | |
46 #end if | |
47 #if str( $adv.minlen ) != "": | |
48 --minReadLen "$adv.minlen" | |
49 #end if | |
50 #if str( $adv.readfilter ) != "": | |
51 --read-filter "$adv.readfilter" | |
52 #end if | |
53 #if str( $adv.region ) != "": | |
54 --aligned-region "$adv.region" | |
55 #end if | |
56 #if str( $adv.spotgroups ) != "": | |
57 --spot-groups "$adv.spotgroups" | |
58 #end if | |
59 #if str( $adv.matepairDist ) != "": | |
60 --matepair-distance "$adv.matepairDist" | |
61 #end if | |
62 #if $adv.clip == "yes": | |
63 --clip | |
64 #end if | |
65 #if str( $outputformat ) == "fasta": | |
66 --fasta | |
67 #end if | |
68 #if $input.input_select=="file": | |
69 "$input.file" > "$output_file" | |
70 #else: | |
71 "$input.accession" > "$output_accession" | |
72 #end if | |
73 ]]> | |
74 </command> | |
75 <version_string>fastq-dump --version</version_string> | |
76 <inputs> | |
77 <expand macro="input_conditional"/> | |
78 <param name="outputformat" type="select" label="select output format"> | |
79 <option value="fastqsanger">fastq</option> | |
80 <option value="fasta">fasta</option> | |
81 </param> | |
82 <section name="adv" title="Advanced Options" expanded="False"> | |
83 <param name="minID" type="integer" label="minimum spot ID" optional="true"/> | |
84 <param name="maxID" type="integer" label="maximum spot ID" optional="true"/> | |
85 <param name="minlen" type="integer" label="minimum read length" optional="true"/> | |
86 <param name="split" type="select" value="yes"> | |
87 <label>split spot by read pairs</label> | |
88 <option value="yes">Yes</option> | |
89 <option value="no">No</option> | |
90 </param> | |
91 <expand macro="alignments"/> | |
92 <expand macro="region"/> | |
93 <expand macro="matepairDist"/> | |
94 <param name="readfilter" type="select" value=""> | |
95 <label>filter by value</label> | |
96 <option value="">None</option> | |
97 <option value="pass">pass</option> | |
98 <option value="reject">reject</option> | |
99 <option value="criteria">criteria</option> | |
100 <option value="redacted">redacted</option> | |
101 </param> | |
102 <param name="spotgroups" type="text" label="filter by spot-groups" optional="true"/> | |
103 <param name="clip" type="select" value="no"> | |
104 <label>apply left and right clips</label> | |
105 <option value="no">No</option> | |
106 <option value="yes">Yes</option> | |
107 </param> | |
108 </section> | |
109 </inputs> | |
110 <outputs> | |
111 <data format="fastq" name="output_accession" label="${input.accession}.${outputformat}"> | |
112 <filter>input['input_select'] == "accession_number"</filter> | |
113 <change_format> | |
114 <when input="outputformat" value="fasta" format="fasta"/> | |
115 </change_format> | |
116 </data> | |
117 <data format="fastq" name="output_file" label="${input.file.name}.${outputformat}"> | |
118 <filter>input['input_select'] == "file"</filter> | |
119 <change_format> | |
120 <when input="outputformat" value="fasta" format="fasta"/> | |
121 </change_format> | |
122 </data> | |
123 </outputs> | |
124 <stdio> | |
125 <exit_code range="127" level="fatal" description="Could not locate fastq-dump binary"/> | |
126 </stdio> | |
127 <tests> | |
128 <test> | |
129 <param name="input_select" value="accession_number"/> | |
130 <param name="outputformat" value="fastqsanger"/> | |
131 <param name="accession" value="SRR925743"/> | |
132 <param name="maxID" value="5"/> | |
133 <output name="output_accession" file="fastq_dump_result.fastq" ftype="fastq"/> | |
134 </test> | |
135 </tests> | |
136 <help> | |
137 This tool extracts reads from SRA archives using fastq-dump. | |
138 The fastq-dump program is developed at NCBI, and is available at | |
139 http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=software. | |
140 @SRATOOLS_ATTRRIBUTION@ | |
141 </help> | |
142 </tool> |