annotate rseqc_macros.xml @ 51:09846d5169fa draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
author iuc
date Tue, 14 Mar 2017 10:23:21 -0400
parents 6b33e31bda10
children 34e4c586e3c0
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6b33e31bda10 Uploaded tar based on https://github.com/lparsons/galaxy_tools/tree/master/tools/rseqc 1a3c419bc0ded7c40cb2bc3e7c87bfb01ddfeba2
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1 <macros>
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09846d5169fa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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3 <token name="@WRAPPER_VERSION@">2.6.4</token>
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5 <xml name="requirements">
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6 <requirements>
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7 <requirement type="package" version="2.6.4">rseqc</requirement>
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8 </requirements>
09846d5169fa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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9 </xml>
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6b33e31bda10 Uploaded tar based on https://github.com/lparsons/galaxy_tools/tree/master/tools/rseqc 1a3c419bc0ded7c40cb2bc3e7c87bfb01ddfeba2
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6b33e31bda10 Uploaded tar based on https://github.com/lparsons/galaxy_tools/tree/master/tools/rseqc 1a3c419bc0ded7c40cb2bc3e7c87bfb01ddfeba2
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11 <xml name="stdio">
6b33e31bda10 Uploaded tar based on https://github.com/lparsons/galaxy_tools/tree/master/tools/rseqc 1a3c419bc0ded7c40cb2bc3e7c87bfb01ddfeba2
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12 <stdio>
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13 <exit_code range="1:" level="fatal" description="An error occured during execution, see stderr and stdout for more information" />
6b33e31bda10 Uploaded tar based on https://github.com/lparsons/galaxy_tools/tree/master/tools/rseqc 1a3c419bc0ded7c40cb2bc3e7c87bfb01ddfeba2
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14 <regex match="[Ee]rror" source="both" description="An error occured during execution, see stderr and stdout for more information" />
6b33e31bda10 Uploaded tar based on https://github.com/lparsons/galaxy_tools/tree/master/tools/rseqc 1a3c419bc0ded7c40cb2bc3e7c87bfb01ddfeba2
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15 </stdio>
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16 </xml>
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18 <!-- Params -->
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19 <xml name="bam_param">
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20 <param name="input" type="data" label="Input .bam file" format="bam" help="(--input-file)"/>
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21 </xml>
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23 <xml name="bam_sam_param">
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24 <param name="input" type="data" label="Input .bam/.sam file" format="bam,sam" help="(--input-file)"/>
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25 </xml>
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27 <xml name="refgene_param">
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28 <param name="refgene" type="data" format="bed" label="Reference gene model" help="(--refgene)"/>
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29 </xml>
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31 <xml name="mapq_param">
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32 <param name="mapq" type="integer" label="Minimum mapping quality" value="30" help="Minimum mapping quality for an alignment to be considered as &quot;uniquely mapped&quot; (--mapq)"/>
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33 </xml>
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35 <xml name="readlength_param">
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36 <param name="readlength" type="integer" value="" label="Alignment length" optional="false" help="Alignment length of read, usually set to the orignial read length (--read-align-length)"/>
09846d5169fa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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37 </xml>
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38
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39 <xml name="readnum_param">
09846d5169fa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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40 <param name="readnum" type="integer" label="Number of aligned reads" value="1000000" help="Number of aligned reads with mismatches used to calculate the mismatch profile (--read-num)"/>
09846d5169fa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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41 </xml>
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43 <xml name="sample_size_param">
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44 <param name="sample_size" type="integer" label="Number of reads sampled from SAM/BAM file (default = 200000)" value="200000" min="1" help="(--sample-size)"/>
09846d5169fa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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45 </xml>
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47 <xml name="min_intron_param">
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48 <param name="min_intron" type="integer" value="50" label="Minimum intron length (bp, default=50)" help="(--min-intron)" />
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49 </xml>
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50
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51 <xml name="layout_param">
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52 <param name="layout" type="select" label="Sequencing layout" help="(--sequencing)">
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53 <option value="SE" selected="true">Single-end</option>
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54 <option value="PE">Paired-end</option>
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55 </param>
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56 </xml>
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57
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58 <xml name="strand_type_param">
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59 <conditional name="strand_type">
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60 <param name="strand_specific" type="select" label="Strand-specific?">
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61 <option value="none" selected="true">None</option>
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62 <option value="pair">Pair-End RNA-seq</option>
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63 <option value="single">Single-End RNA-seq</option>
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64 </param>
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65 <when value="pair">
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66 <param name="pair_type" type="select" display="radio" label="Pair-End Read Type (format: mapped --> parent)" help="(--strand)">
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67 <option value="sd" selected="true"> read1 (positive --> positive; negative --> negative), read2 (positive --> negative; negative --> positive)</option>
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68 <option value="ds">read1 (positive --> negative; negative --> positive), read2 (positive --> positive; negative --> negative)</option>
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69 </param>
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70 </when>
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71 <when value="single">
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72 <param name="single_type" type="select" display="radio" label="Single-End Read Type (format: mapped --> parent)" help="(--strand)">
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73 <option value="s" selected="true">positive --> positive; negative --> negative</option>
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74 <option value="d">positive --> negative; negative --> positive</option>
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75 </param>
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76 </when>
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77 <when value="none"></when>
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78 </conditional>
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79 </xml>
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80
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81 <xml name="multihits_param">
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82 <conditional name="multihits_type">
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83 <param name="multihits_type_selector" type="select" label="Reads with multiple hits" help="(--skip-multi-hits)">
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84 <option value="use_multihits" selected="true">Count Mutliple Hit Reads</option>
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85 <option value="skip_multihits">Skip Multiple Hit Reads/Only Use Uniquely Mapped Reads</option>
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86 </param>
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87 <when value="skip_multihits">
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88 <expand macro="mapq_param" />
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89 </when>
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90 <when value="use_multihits" />
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91 </conditional>
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92 </xml>
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93
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94 <xml name="rscript_output_param">
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95 <param name="rscript_output" type="boolean" value="false" label="Output R-Script"
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96 help="Output the R-Script used to generate the plots" />
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97 </xml>
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98
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99
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100 <!-- Output -->
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101
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102 <xml name="pdf_output_data" token_filename="output.pdf">
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103 <data format="pdf" name="outputpdf" from_work_dir="@FILENAME@" label="${tool.name} on ${on_string} (pdf)" />
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104 </xml>
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105
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106 <xml name="xls_output_data" token_filename="output.xls">
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107 <data format="xls" name="outputxls" from_work_dir="@FILENAME@" label="${tool.name} on ${on_string} (xls)" />
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108 </xml>
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109
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110 <xml name="rscript_output_data" token_filename="output.r">
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111 <data format="txt" name="outputr" from_work_dir="@FILENAME@" label="${tool.name} on ${on_string} (rscript)">
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112 <filter>rscript_output</filter>
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113 </data>
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114 </xml>
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115
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116 <!-- Command -->
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117 <token name="@MULTIHITS@">
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118 <![CDATA[
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119 #if str($multihits_type.multihits_type_selector) == "skip_multihits"
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120 --skip-multi-hits
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121 --mapq=${multihits_type.mapq}
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122 #end if
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123 ]]>
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124 </token>
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125
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126 <token name="@ABOUT@">
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127
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128 -----
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129
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130 About RSeQC
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131 +++++++++++
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132
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133 The RSeQC_ package provides a number of useful modules that can comprehensively
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134 evaluate high throughput sequence data especially RNA-seq data. "Basic modules"
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135 quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC
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136 bias, while "RNA-seq specific modules" investigate sequencing saturation status
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137 of both splicing junction detection and expression estimation, mapped reads
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138 clipping profile, mapped reads distribution, coverage uniformity over gene
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139 body, reproducibility, strand specificity and splice junction annotation.
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140
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141 The RSeQC package is licensed under the GNU GPL v3 license.
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142
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143 .. image:: $PATH_TO_IMAGES/logo.png
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144
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145 .. _RSeQC: http://rseqc.sourceforge.net/
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146
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147
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148 </token>
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149
49
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150 <xml name="citations">
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151 <citations>
51
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152 <citation type="doi">10.1093/bioinformatics/bts356</citation>
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153 </citations>
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154 </xml>
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155 </macros>