Mercurial > repos > ning > ebseq
changeset 35:8b85a39cb625 draft
Uploaded
author | ning |
---|---|
date | Mon, 16 Jun 2014 16:22:56 -0400 |
parents | 360c058ed13a |
children | 7be90b1366d5 |
files | EBGeneTwoCondTest.xml |
diffstat | 1 files changed, 39 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/EBGeneTwoCondTest.xml Mon Jun 16 16:22:56 2014 -0400 @@ -0,0 +1,39 @@ +<tool id="EBGeneTwoCondTest" name="Gene level DE test across two conditions"> + <description>Runs EBSeq to find DE genes across two conditions</description> + <command>R --quiet --slave --file=$GALAXY_ROOT_DIR/tools/EBSeq/EBGeneTwoCondTest.R --args $Gene_Expression $First_Row_Sample_Names $Conditions $Target_FDR $Output $Sorted_Output $Sorted_Output_with_target_FDR $Sizes 1>NUL 2>NUL</command> + <inputs> + <param name="Gene_Expression" type="data" format="tabular" label="Gene Expression (tab delimited, please use the unnormalized values, e.g. expected counts from RSEM)"/> + <param name="First_Row_Sample_Names" type="select" format="text"> + <label>The First Row is Sample Names?</label> + <option value="y">Yes</option> + <option value="n">No</option> + </param> + <param name="Conditions" type="text" size="60" value="C1,C2" label="Enter which condition each + sample belongs to (separated by comma, no space please)." + help="Sample with condition that comes first alphabetically will be in numerator (C1 relative to C2)."/> + <param name="Target_FDR" type="text" format="tabular" value="0.05" label="Target FDR" + /> + </inputs> + <outputs> + <data format="tabular" name="Output" label="Output"/> + <data format="tabular" name="Sorted_Output" label="Output sorted by PPDE"/> + <data format="tabular" name="Sorted_Output_with_target_FDR" label="Sorted output with FDR cutoff"/> + <data format="tabular" name="Sizes" label="Normalization factors"/> + </outputs> + + <help> +The input Conditions should have exactly two levels. The length of the Condition vector should be exactly the same as the number of +columns in the data file (except the gene names column). + +Four output files will be generated. Each of the first 3 files contains Posterior probability of being +DE (PPDE), Fold Change (RealFC), Posterior Fold Change (PostFC) and normalized gene expressions. +The Four files are: +Genes with the same order as in input file; +Genes sorted by PPDE; DE Genes under target FDR (PPDE>=TargetFDR) +and sorted by PPDE; +Library size factor for each sample. + +</help> + + +</tool>