changeset 10:9adf2362f302 draft

Uploaded
author ning
date Fri, 15 Mar 2013 18:22:45 -0400
parents c20070b11b55
children 696a1f7e23fd
files EBGeneTwoCondTest.xml
diffstat 1 files changed, 39 insertions(+), 0 deletions(-) [+]
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/EBGeneTwoCondTest.xml	Fri Mar 15 18:22:45 2013 -0400
@@ -0,0 +1,39 @@
+<tool id="EBGeneTwoCondTest" name="Gene level DE test across two conditions">
+  <description>Runs EBSeq to find DE genes across two conditions</description>
+  <command>R --quiet --slave --file=$GALAXY_ROOT_DIR/tools/EBSeq/EBGeneTwoCondTest.R --args $Gene_Expression $First_Row_Sample_Names $Conditions $Target_FDR $Output $Sorted_Output $Sorted_Output_with_target_FDR $Sizes</command>
+  <inputs>
+		<param name="Gene_Expression" type="data" format="tabular" label="Gene Expression (tab eliminated, please use the unnormalized values)"/>
+		<param name="First_Row_Sample_Names" type="select" format="text">
+			<label>The First Row is Sample Names?</label>
+			<option value="y">Yes</option>
+			<option value="n">No</option>
+			</param>
+		<param name="Conditions" type="text" size="60" value="C1,C2" label="Enter which condition each
+			sample belongs to (seperated by comma, no space please)." 
+			help=""/>
+		<param name="Target_FDR" type="text" format="tabular" value="0.05" label="Target FDR"
+			/>
+  </inputs>
+  <outputs>
+		<data format="tabular" name="Output" />
+		<data format="tabular" name="Sorted_Output" />
+		<data format="tabular" name="Sorted_Output_with_target_FDR" />
+                <data format="tabular" name="Sizes" />
+  </outputs>
+
+  <help>
+The input Conditions should have exactly two levels. The length of the Condition vector should be exactly the same as the number of 
+columns in the data file (except the gene names column).
+
+Four output files will be generated. Each file contains Posterior probability of being 
+DE (PPDE), Fold Change (RealFC), Posterior Fold Change (PostFC) and normalized gene expressions.
+The Four files are:
+Genes with the same order as in input file;
+Genes sorted by PPDE; DE Genes under target FDR (PPDE>=TargetFDR) 
+and sorted by PPDE.
+Library size factor for each sample.
+
+</help>
+
+
+</tool>