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annotate callcodonvar.xml @ 9:18aad692772a draft default tip

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planemo upload for repository https://github.com/phac-nml/quasitools commit 5a9e4c9a582828654893166caf20576f5e0c418e
author nml
date Mon, 20 Jun 2022 20:06:36 +0000
parents 9def47f3c1e4
children
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18aad692772a planemo upload for repository https://github.com/phac-nml/quasitools commit 5a9e4c9a582828654893166caf20576f5e0c418e
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1 <tool id="callcodonvar" name="Codon Variants" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
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2 <description>Identifies codon variants and non-synonymous/synonymous mutations</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements"/>
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7 <command detect_errors="exit_code"><![CDATA[
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9 cat $input_genes &&
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11 ln -s $input_bam '${input_bam.name}' &&
18aad692772a planemo upload for repository https://github.com/phac-nml/quasitools commit 5a9e4c9a582828654893166caf20576f5e0c418e
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12 ln -s $input_bam.metadata.bam_index '${input_bam.name}.bai' &&
18aad692772a planemo upload for repository https://github.com/phac-nml/quasitools commit 5a9e4c9a582828654893166caf20576f5e0c418e
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18aad692772a planemo upload for repository https://github.com/phac-nml/quasitools commit 5a9e4c9a582828654893166caf20576f5e0c418e
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14 quasitools call codonvar '${input_bam.name}' $ref_file $offset $input_genes
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16 #if $var_file:
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17 $var_file
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18 #end if
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19
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20 #if $error_rate:
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21 -e $error_rate
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22 #end if
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24 -o output.csv
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26 ]]></command>
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27 <inputs>
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28 <param name="input_bam" type="data" format="bam" optional="false" label="Bam file" />
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29 <param name="ref_file" type="data" format="fasta" optional="false" label="Reference file" />
71976cfc9022 planemo upload for repository https://github.com/phac-nml/quasitools commit 8a264400a75945e2e0fdd5a08c007a8b1b7a2f0f
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30 <param name="offset" type="integer" optional="false" label="Offset" min="0" value="0"/>
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31 <param name="input_genes" type="data" format="bed" optional="false" label="Gene file" />
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32 <param name="error_rate" type="float" optional="true" min="0" max="1" label="Error rate" value="0.0021" help="Estimated sequencing error rate. Defaults to 0.0021." />
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33 <param name="var_file" type="data" format="vcf" optional="true" label="Variants file" help="Not required. If not supplied, a variants file will be generated using the input bam file." />
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34 </inputs>
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35 <outputs>
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36 <data format="csv" name="output" from_work_dir="output.csv" />
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37 </outputs>
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38 <tests>
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39 <test>
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40 <param name="input_bam" value="align.bam" />
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41 <param name="ref_file" value="hxb2_pol.fas" />
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42 <param name="offset" value="1269"/>
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43 <param name="input_genes" ftype="bed" value="hxb2_pol.bed" />
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44 <output name="output" >
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45 <assert_contents>
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46 <has_text text="#gene,nt position (gene),nt start position,nt end position,ref codon,mutant codon,ref AA,mutant AA,coverage,mutant frequency,mutant type,NS count,S count" />
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47 <has_text text="RT,1566-2885,1872,1874,aaa,aaC,K,N,154,7.79,NS,1.0000,0.0000" />
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48 </assert_contents>
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49 </output>
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50 </test>
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51 </tests>
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52 <help><![CDATA[
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54 Codon Variants
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55 ==============
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56
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57 Call codon variants for a given BAM. A report is generated that details nucleotide variants within a
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58 codon and the resulting AA variants. The report indicates whether the nucleotide variants correspond to
71976cfc9022 planemo upload for repository https://github.com/phac-nml/quasitools commit 8a264400a75945e2e0fdd5a08c007a8b1b7a2f0f
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59 a synonymous or non-synonymous mutation.
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61 ]]></help>
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62 <expand macro="citations" />
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63 </tool>