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annotate drmutations.xml @ 4:8cdffc02d2e2 draft

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planemo upload for repository https://github.com/phac-nml/quasitools commit 19e0f266ee455b82432e2d7b987b35de8c7a14d5
author nml
date Wed, 25 Apr 2018 10:38:00 -0400
parents a7093d5933a8
children b69e898b8109
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71976cfc9022 planemo upload for repository https://github.com/phac-nml/quasitools commit 8a264400a75945e2e0fdd5a08c007a8b1b7a2f0f
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1 <tool id="drmutations" name="Drug Resistance Mutations" version="0.1.0">
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2 <description></description>
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3 <requirements>
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8cdffc02d2e2 planemo upload for repository https://github.com/phac-nml/quasitools commit 19e0f266ee455b82432e2d7b987b35de8c7a14d5
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4 <requirement type="package" version="0.3.1">quasitools</requirement>
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5 </requirements>
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6 <command detect_errors="exit_code"><![CDATA[
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8 ln -f -s ${input_bam.metadata.bam_index} ${input_bam}.bai &&
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9 quasitools drmutations $input_bam $ref_file $var_file $input_genes
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11 #if $mutation_db:
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12 $mutation_db
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13 #end if
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15 #if $min_freq:
71976cfc9022 planemo upload for repository https://github.com/phac-nml/quasitools commit 8a264400a75945e2e0fdd5a08c007a8b1b7a2f0f
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16 -f $min_freq
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17 #end if
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19 #if $reporting_thres:
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20 -t $reporting_thres
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21 #end if
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23 -o output.csv
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25 ]]></command>
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26 <inputs>
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27 <param name="input_bam" type="data" format="bam" optional="false" label="Bam file" />
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28 <param name="ref_file" type="data" format="fasta" optional="false" label="Reference file" />
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29 <param name="var_file" type="data" format="vcf" optional="false" label="Variants file" />
71976cfc9022 planemo upload for repository https://github.com/phac-nml/quasitools commit 8a264400a75945e2e0fdd5a08c007a8b1b7a2f0f
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30 <param name="input_genes" type="data" format="bed" optional="false" label="Gene file" />
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31 <param name="mutation_db" type="data" format="tsv" optional="false" label="Mutation DB" />
71976cfc9022 planemo upload for repository https://github.com/phac-nml/quasitools commit 8a264400a75945e2e0fdd5a08c007a8b1b7a2f0f
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32 <param name="min_freq" type="float" optional="true" min="0" max="1" label="Minimum frequency" value="0.01" help="The minimum required frequency. Defaults to 0.01." />
71976cfc9022 planemo upload for repository https://github.com/phac-nml/quasitools commit 8a264400a75945e2e0fdd5a08c007a8b1b7a2f0f
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33 <param name="reporting_thres" type="integer" min="1" max="20" optional="true" label="Reporting threshold" value="1"
71976cfc9022 planemo upload for repository https://github.com/phac-nml/quasitools commit 8a264400a75945e2e0fdd5a08c007a8b1b7a2f0f
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34 help="The minimum percentage required for an entry in the drug resistant report. Defaults to 1."/>
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35 </inputs>
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36 <outputs>
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37 <data format="csv" name="output" from_work_dir="output.csv" />
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38 </outputs>
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39 <tests>
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40 <test>
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41 <param name="input_bam" value="align.bam" />
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42 <param name="ref_file" value="hxb2_pol.fas" />
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43 <param name="var_file" value="nt_variants.vcf" />
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44 <param name="input_genes" ftype="bed" value="hxb2_pol.bed" />
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45 <param name="mutation_db" value="mutation_db.tsv" />
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46 <output name="output" >
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47 <assert_contents>
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48 <has_text text="Chromosome,Gene,Category,Surveillance,Wildtype,Position,Mutation,Mutation Frequency,Coverage" />
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49 <has_text text="hxb2_pol,RT,NNRTI,Yes,K,103,N,7.79,154" />
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50 </assert_contents>
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51 </output>
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52 </test>
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53 </tests>
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54 <help><![CDATA[
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56 Drug Resistance Mutations
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57 =========================
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58
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59 Generates a report detailing the drug resistant mutations found, above the reporting threshold (default: 1%).
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61 ]]></help>
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62 <citations>
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63 </citations>
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64 </tool>