Mercurial > repos > peterjc > effectivet3
annotate tools/effectiveT3/effectiveT3.py @ 9:512530020360 draft
v0.0.18 Internal changes to command line handling
author | peterjc |
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date | Tue, 16 May 2017 09:17:17 -0400 |
parents | 60a9b3f760cc |
children | a46d7861c32c |
rev | line source |
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1 #!/usr/bin/env python |
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2 """Wrapper for EffectiveT3 v1.0.1 for use in Galaxy. |
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3 |
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4 This script takes exactly five command line arguments: |
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5 * model name (e.g. TTSS_STD-1.0.1.jar) |
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6 * threshold (selective or sensitive) |
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7 * an input protein FASTA filename |
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8 * output tabular filename |
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9 |
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10 It then calls the standalone Effective T3 v1.0.1 program (not the |
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11 webservice), and reformats the semi-colon separated output into |
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12 tab separated output for use in Galaxy. |
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13 """ |
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14 import os |
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15 import subprocess |
9 | 16 import sys |
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17 |
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18 # The Galaxy auto-install via tool_dependencies.xml will set this environment variable |
8 | 19 effective_t3_dir = os.environ.get("EFFECTIVET3", "/opt/EffectiveT3/") |
20 effective_t3_jar = os.path.join(effective_t3_dir, "TTSS_GUI-1.0.1.jar") | |
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21 |
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22 if "-v" in sys.argv or "--version" in sys.argv: |
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23 # TODO - Get version of the JAR file dynamically? |
8 | 24 print("Wrapper v0.0.17, TTSS_GUI-1.0.1.jar") |
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25 sys.exit(0) |
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26 |
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27 if len(sys.argv) != 5: |
8 | 28 sys.exit("Require four arguments: model, threshold, input protein FASTA file & output tabular file") |
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29 |
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30 model, threshold, fasta_file, tabular_file = sys.argv[1:] |
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31 |
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32 if not os.path.isfile(fasta_file): |
8 | 33 sys.exit("Input FASTA file not found: %s" % fasta_file) |
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34 |
9 | 35 if threshold not in ["selective", "sensitive"] and not threshold.startswith("cutoff="): |
8 | 36 sys.exit("Threshold should be selective, sensitive, or cutoff=..., not %r" % threshold) |
37 | |
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38 |
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39 def clean_tabular(raw_handle, out_handle): |
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40 """Clean up Effective T3 output to make it tabular.""" |
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41 count = 0 |
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42 positive = 0 |
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43 errors = 0 |
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44 for line in raw_handle: |
9 | 45 if not line or line.startswith("#") or line.startswith("Id; Description; Score;"): |
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46 continue |
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47 assert line.count(";") >= 3, repr(line) |
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48 # Normally there will just be three semi-colons, however the |
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49 # original FASTA file's ID or description might have had |
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50 # semi-colons in it as well, hence the following hackery: |
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51 try: |
8 | 52 id_descr, score, effective = line.rstrip("\r\n").rsplit(";", 2) |
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53 # Cope when there was no FASTA description |
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54 if "; " not in id_descr and id_descr.endswith(";"): |
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55 id = id_descr[:-1] |
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56 descr = "" |
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57 else: |
8 | 58 id, descr = id_descr.split("; ", 1) |
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59 except ValueError: |
8 | 60 sys.exit("Problem parsing line:\n%s\n" % line) |
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61 parts = [s.strip() for s in [id, descr, score, effective]] |
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62 out_handle.write("\t".join(parts) + "\n") |
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63 count += 1 |
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64 if float(score) < 0: |
8 | 65 errors += 1 |
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66 if effective.lower() == "true": |
8 | 67 positive += 1 |
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68 return count, positive, errors |
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69 |
8 | 70 |
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71 def run(cmd): |
9 | 72 """Run the command line string via subprocess.""" |
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73 # Avoid using shell=True when we call subprocess to ensure if the Python |
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74 # script is killed, so too is the child process. |
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75 try: |
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76 child = subprocess.Popen(cmd, stdout=subprocess.PIPE, stderr=subprocess.PIPE) |
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77 except Exception, err: |
8 | 78 sys.exit("Error invoking command:\n%s\n\n%s\n" % (" ".join(cmd), err)) |
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79 # Use .communicate as can get deadlocks with .wait(), |
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80 stdout, stderr = child.communicate() |
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81 return_code = child.returncode |
7 | 82 if return_code or stderr.startswith("Exception in thread"): |
8 | 83 cmd_str = " ".join(cmd) # doesn't quote spaces etc |
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84 if stderr and stdout: |
8 | 85 sys.exit("Return code %i from command:\n%s\n\n%s\n\n%s" % (return_code, cmd_str, stdout, stderr)) |
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86 else: |
8 | 87 sys.exit("Return code %i from command:\n%s\n%s" % (return_code, cmd_str, stderr)) |
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88 |
7 | 89 |
8 | 90 if not os.path.isdir(effective_t3_dir): |
91 sys.exit("Effective T3 folder not found: %r" % effective_t3_dir) | |
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92 |
8 | 93 if not os.path.isfile(effective_t3_jar): |
94 sys.exit("Effective T3 JAR file not found: %r" % effective_t3_jar) | |
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95 |
8 | 96 if not os.path.isdir(os.path.join(effective_t3_dir, "module")): |
97 sys.exit("Effective T3 module folder not found: %r" % os.path.join(effective_t3_dir, "module")) | |
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98 |
8 | 99 effective_t3_model = os.path.join(effective_t3_dir, "module", model) |
100 if not os.path.isfile(effective_t3_model): | |
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101 sys.stderr.write("Contents of %r is %s\n" |
8 | 102 % (os.path.join(effective_t3_dir, "module"), |
103 ", ".join(repr(p) for p in os.listdir(os.path.join(effective_t3_dir, "module"))))) | |
104 sys.stderr.write("Main JAR was found: %r\n" % effective_t3_jar) | |
105 sys.exit("Effective T3 model JAR file not found: %r" % effective_t3_model) | |
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106 |
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107 # We will have write access whereever the output should be, |
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108 temp_file = os.path.abspath(tabular_file + ".tmp") |
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109 |
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110 # Use absolute paths since will change current directory... |
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111 tabular_file = os.path.abspath(tabular_file) |
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112 fasta_file = os.path.abspath(fasta_file) |
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113 |
8 | 114 cmd = ["java", "-jar", effective_t3_jar, |
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115 "-f", fasta_file, |
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116 "-m", model, |
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117 "-t", threshold, |
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118 "-o", temp_file, |
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119 "-q"] |
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120 |
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121 try: |
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122 # Must run from directory above the module subfolder: |
8 | 123 os.chdir(effective_t3_dir) |
124 except Exception: | |
125 sys.exit("Could not change to Effective T3 folder: %s" % effective_t3_dir) | |
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126 |
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127 run(cmd) |
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128 |
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129 if not os.path.isfile(temp_file): |
8 | 130 sys.exit("ERROR - No output file from Effective T3") |
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131 |
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132 out_handle = open(tabular_file, "w") |
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133 out_handle.write("#ID\tDescription\tScore\tEffective\n") |
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134 data_handle = open(temp_file) |
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135 count, positive, errors = clean_tabular(data_handle, out_handle) |
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136 data_handle.close() |
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137 out_handle.close() |
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138 |
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139 os.remove(temp_file) |
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140 |
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141 if errors: |
8 | 142 print("%i sequences, %i positive, %i errors" |
143 % (count, positive, errors)) | |
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144 else: |
8 | 145 print("%i/%i sequences positive" % (positive, count)) |
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146 |
8 | 147 if count and count == errors: |
148 # Galaxy will still allow them to see the output file | |
149 sys.exit("All your sequences gave an error code") |