Mercurial > repos > peterjc > effectivet3
annotate tools/effectiveT3/effectiveT3.xml @ 4:f7ce32e13bc6 draft
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author | peterjc |
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date | Wed, 18 Sep 2013 06:11:36 -0400 |
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1 <tool id="effectiveT3" name="Effective T3" version="0.0.12"> |
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2 <description>Find bacterial effectors in protein sequences</description> |
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3 <requirements> |
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4 <requirement type="package" version="1.0.1">effectiveT3</requirement> |
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5 </requirements> |
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6 <version_command interpreter="python">effectiveT3.py --version</version_command> |
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7 <command interpreter="python"> |
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8 effectiveT3.py $module.fields.path |
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9 #if $restrict.type=="cutoff": |
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10 cutoff=$restrict.cutoff |
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11 #else: |
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12 $restrict.type |
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13 #end if |
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14 $fasta_file $tabular_file</command> |
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15 <stdio> |
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16 <!-- Anything other than zero is an error --> |
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17 <exit_code range="1:" /> |
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18 <exit_code range=":-1" /> |
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19 </stdio> |
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20 <inputs> |
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21 <param name="fasta_file" type="data" format="fasta" label="FASTA file of protein sequences"/> |
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22 <param name="module" type="select" display="radio" label="Classification module"> |
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23 <options from_file="effectiveT3.loc"> |
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24 <column name="value" index="0"/> |
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25 <column name="name" index="1"/> |
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26 <column name="path" index="2"/> |
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27 </options> |
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28 </param> |
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29 <conditional name="restrict"> |
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30 <param name="type" type="select" label="Cut-off setting"> |
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31 <option value="selective">Selective (threshold set in module)</option> |
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32 <option value="sensitive">Sensitive (threshold set in module)</option> |
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33 <option value="cutoff">User defined cut-off</option> |
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34 </param> |
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35 <when value="selective" /> |
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36 <when value="sensitive" /> |
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37 <when value="cutoff" > |
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38 <param name="cutoff" type="float" min="0" max="1" label="Cut-off" value="" help="Threshold cut-off between 0 and 1" /> |
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39 </when> |
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40 </conditional> |
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41 </inputs> |
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42 <outputs> |
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43 <data name="tabular_file" format="tabular" label="Effective T3 $module.value_label on ${on_string}" /> |
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44 </outputs> |
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45 <tests> |
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46 <test> |
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47 <param name="fasta_file" value="four_human_proteins.fasta" ftype="fasta" /> |
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48 <param name="module" value="animal" /> |
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49 <param name="type" value="selective" /> |
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50 <output name="tabular_file" file="four_human_proteins.effectiveT3.tabular" ftype="tabular" /> |
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51 </test> |
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52 <test> |
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53 <param name="fasta_file" value="empty.fasta" ftype="fasta" /> |
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54 <param name="module" value="plant" /> |
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55 <param name="type" value="sensistive" /> |
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56 <output name="tabular_file" file="empty_effectiveT3.tabular" ftype="tabular" /> |
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57 </test> |
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58 </tests> |
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59 <help> |
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60 |
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61 **What it does** |
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62 |
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63 This calls the command line Effective T3 v1.0.1 tool for prediction of bacterial effector proteins. |
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64 |
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65 The input is a FASTA file of protein sequences, and the output is tabular with four columns (one row per protein): |
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66 |
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67 ====== ============================================================================== |
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68 Column Description |
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69 ------ ------------------------------------------------------------------------------ |
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70 1 Sequence identifier |
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71 2 Sequence description (from the FASTA file) |
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72 3 Score (between 0 and 1, or negative for an error such as a very short peptide) |
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73 4 Predicted effector (true/false) |
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74 ====== ============================================================================== |
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75 |
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76 |
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77 **References** |
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78 |
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79 If you use this Galaxy tool in work leading to a scientific publication please |
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80 cite the following papers: |
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81 |
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82 Peter J.A. Cock, Björn A. Grüning, Konrad Paszkiewicz and Leighton Pritchard (2013). |
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83 Galaxy tools and workflows for sequence analysis with applications |
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84 in molecular plant pathology. PeerJ 1:e167 |
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85 http://dx.doi.org/10.7717/peerj.167 |
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86 |
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87 Jehl, Arnold and Rattei (2011). |
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88 Effective - a database of predicted secreted bacterial proteins |
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89 Nucleic Acids Research, 39(Database issue), D591-5. |
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90 http://dx.doi.org/10.1093/nar/gkq1154 |
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91 |
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92 Arnold, Brandmaier, Kleine, Tischler, Heinz, Behrens, Niinikoski, Mewes, Horn and Rattei (2009). |
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93 Sequence-based prediction of type III secreted proteins. |
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94 PLoS Pathog. 5(4):e1000376. |
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95 http://dx.doi.org/10.1371/journal.ppat.1000376 |
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96 |
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97 See also http://effectors.org/ |
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98 |
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99 This wrapper is available to install into other Galaxy Instances via the Galaxy |
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100 Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/effectivet3 |
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101 </help> |
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102 </tool> |