Mercurial > repos > peterjc > mira4_assembler
view test-data/U13small_m.mira4_de_novo.mira @ 4:1713289d9908 draft default tip
v0.0.11 tweak for use with bioconda dependencies
| author | peterjc |
|---|---|
| date | Thu, 10 Aug 2017 11:09:10 -0400 |
| parents | a4f602cc3aa9 |
| children |
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@Version 2 0 @Program MIRALIB @ReadGroup @RG ID 1 @RG technology Sanger @RG strainname StrainX @RG segmentnaming sanger @EndReadGroup CO MIRA_rep_c1 NR 11 LC 1533 CT MIRA 1 5 = . Note=Assembled with MIRA CT H454 175 175 = . Note=No edit. PL: 3 RS: 2 RL: 1 RWTS: 0 EL: 1 CT H454 664 664 = . Note=No edit. PL: 2 RS: 6 RL: 0 RWTS: 0 EL: 0 CT DGPc 1483 1483 = . Note=*: 1 T: 1 - 50%25 CT H454 1483 1483 = . Note=No edit. PL: 3 RS: 1 RL: 0 RWTS: 0 EL: 0 CT DGPc 1513 1513 = . Note=*: 1 A: 1 - 50%25 CT H454 1513 1513 = . Note=No edit. PL: 2 RS: 1 RL: 0 RWTS: 0 EL: 0 CS aaatttcataaaatttctcatttcaaaaacgagaaaattaaggccttgcggggttaaatcgttatcaagtaatcagtcggctcactgcccagtgtggacactggccaatattatggcaccagcctttgagaaaaggaaacctttttttgaatccatctccctgacctggggttt*gggttgggttttatcagcatagggtgacaggcaagttcaatctgattggatcctggatcccatcatggggtatctgttcctaaatcattccctgatcctcggtcagagcgagcgcttaggttcccgctgcagttaaacacctggttcatctgggcatactaaggttatgactttccacctgggagtccacgacaactgaaaaaactcaaaacttctttaagtagaagttgagcctgaagctgaggcagctgtttcagagaggaggaggaggaggagatagcaaatgtcaagggatttccatttctctttcaatgccaacatacttcagggcatcagcctggctgtatctgtaatcaaaaaacagttcttcgcaagtctggatggctctcctgacaaaaacacctatcctgtgatcaccattaaccatcataacttttgcatagcagtttggatttaccgaatgatttgcaaaacgaattttgttacccttgcaggttgcatccaccacaaaatcattgttcaagttgaacagaaagctgcacatgtatttatcacacactttccctcttccgtcagcttagcttcaggaacctcgagtactgtgggcaatttagaaaaagaaaatgccgtttgaaattctgaatttgcaaagtactataagaataatttatagcaatgagtttaaaactcaactttttattgccttctcaccagctgcaaagtgttttgtaccagtgaatttttgcaataatgcagtatggtatatttttcaactttgaataaagaatacttgaaattgaaaaaaaaaattgagtctgatgggtctagtgtgtttataaaatgacttaagatcccacagttattttaaagtaatacattactcaactggtattcattttgatgccattattttattttataaaactagtcatccttgcagaagtgtatctgattaactcttgacaactactttgcctattttattaacagctgcatttaacccacgtgacaggataactaatttctattgtttttgtatttttgcctcctccttccaagatagcagtagagtactgtgttaaatcttgcttcagaagagcttgtttaaaatagttataaagaaagtaaaatacatagaaaaacacgttataattttaaattaaaaaagaaaaaagatgtctactctgaataaattatgcatacatataaggtctgaacaaaaacatgaaaagatgggctctgagtatcaattttaaaaatctttaccagtataacattactcaaacaaaaattaaaatttaatataa*ttaagtacatattccaagacaacaaatgg*accttgactgttaattctgg CQ 76ABAAEDGJC??>?BCGCHDCCDCCCCB=:<:636?@@<A@==@ADEEA=CFBBCDCAD@EECDDCGED?AAAA?EBAB@?DECCCCBBGDECCAD=67@B@AEDFDDDCDDGGCCDCCDCKQNWNNOKLMJKMVJKPUb_`ff\W=LKLLRMXW\e`f[\URVSV^bcda`Y5cmea`eghb\b`__Y^Q`aadgja_aabefebbd]_ghfdfedldebcVVY\\^e_]_`_cfebfegjhg][__ec[Z`W\]dbbibbhadXc`\U^]aegjfgkde][b^Z^`dZ\bbaRRT[_ehggkkcfhbbdecdhcdfbbfebfeagbhdedgkiccegdebdehfgba]\]]aded]\X`]`bfgdckegejccebfaifkjffddddhijgigggffeffcdcbfaegc`bbbbdfg`cheefjhedffkfgfffeehdgkgghmceddbagjdgjgggjgieiddcafhf`dhgfifikgighmihggkjgidhdleghjdjiegigkhjefglgjhkheihhjiifkmkhjiidhljjjeiigdeedc^dkgf\]ecjififdeekjhieeeiihhhddhf`bbafdkefilgfkilioghmhjhhfhghhmegc[lfbhieibeec`cjcbeeccgkggghfifbeagfjifcdihdYhfgfhdhfacbhfhehehgkkgehgbhledfdeddcV`addcceeakjfhjemfikgfcgedfjfenlej`fh_defee`cefi`diic[c`egiee_b]c]`^d\^W]`jbhffleefhje^`H`caddcgcciffhfhmddee_egfge`cggfckjfddda]WZbh``]a[cZZed^j]\c`Y[V_f`ajffddf[\]\`cfgijaVW;SWP]^afcb\VT[X[]_^`^_\\jhkda`eea`fd`Z`U``ebeg]`hhgahfe]gb_f`QOPbcmihmd^c``[N[_`iggc_ab_g`daddecdfdbffkpjmkigecc`\VUQQW[X]XYPY]`gdcbclheecgjedaeYheac]b`_ddb^`ba[ahhigkidgddeccdddbhcRYG]^gdghbcdgghgh^ba`_fb[acWd`]efedfedVelklkde`a_cabc`cgfc[^b`_^Xd]a^]debgf\`b^^^_XPZU[c]bf`[`X`]]gdceba]hdecbabbdgc\S_S\[^``ac_`aX]]bfhgbcc`bb`bc_^cgbe__[YYa[_\X\ZY\_^__\]cciacd^_`gcdejg[fY]_\`__c``a``ZYW\````^]c]]QWRSbdfghbadaegffcabba_baaa_[_Y_]Z\YTV^abbd``_]_WXf[c[aa`dabcc`edbeadabaabbb``bYKXTMTUKSPZ\```]`bacb`^_RLRKHOPZYQHGL??CEO[YZ_[^YWOJY\fdVVMNQ?<C6OVXb_W[[VVR_SPMOQPQ[VYXZVNN9GLMYYca`a_Y_]XTNQLLRRRKQUONIIMPT[aTNRVWAbYXTQLGNGJENJSPJJHLJPGM=HOAC;/@OIQYTQISQ\XT>DHMJ>E \\ RD U13a03c03.t1 RG 1 RS XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTAACAAATCTCATGGTTTAAAAATTTCATAAAATTTCTCATTTCAAAAACGAGAAAATTAAGGCCTTGCGGGGTTAAATCGTTATCAAGTAATCAGTCGGCTCACTGCCCAGTGTGGACACTGGCCAATATTATGGCACCAGCCTTTGAGAAAAGGAAACCTTTTTTTGAATCCATCTCCCTGACCTGGGGTTT*GGGTTGGGTTTTATCACCATAGGGTGACAGGCAAGTTCAATCTGATTGGATCCTGGATCCCATCATGGGGTATCTGTTCCTAAATCATTCCCTGATCCTCGGTCAGAGCGAGCGCTTAGGTTCCCGCTGCAGTTAAACACCTGGTTCATCTGGGCATACTAAGGTTATGACTTTCCACCTGGGAGTCCACGACAACTGAAAAAACTCAAAACTTCTTTAAGTAGAAGTTGAGCCTGAAGCTGAGGCAGCTGTTTCAGAGAGGAGGAGGAGGAGGAGATaGCaaATGTCaAGGGATTTCCATTTCTCTTTCAATGCCAACATACTTCAGGGCATCAGCCTGGCTGTATCTGTAATCAAAAAACAgTTCTTCGCAAGTCTGGATGGCTCTCCCTGAACAAAAAACACCTATCCCTGGTTGAATCACCCCATTAAACCCAATCCCTAAACTTTTTGGCCATAAACCAATTTTTGGGGAATTTTTCTCCCAAAAATTGAATATTTTGCCCAAAAAACCGCAAAAATATTTGTTGTTTTTAACCCCCCTCTTTGTCCCCGAGGGTGTTTTGCCCTCTTCCCCATCCCCCTCTCTAAAAAAAATTCATTTCTTTGTTTTCTCCAAAAAGTTTTTTGATAAAAACAAGAAAAAAAAGCGCCCCGCCCCTCCTCATTTGTTTTTTTTTTTTTATTTTACAACCACACCCACACCTTTTTTTCTCCCCTCTCCTCTCTCTCCCCCTTCTTCACCCCCCTCTTTTTCTCTTTTTTTGGGGGGGGGGAGAGAGAGAGAAAAAAAAATTTTTTCTCCT RQ !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!;8777==----61511EAAE,=>=>>>@AE@====>AAEAEAAAAAAABA83:*3.1=>>:?>8;>?AB@?8;;??8@599,BB<A@@DB?=???><C>?@><ACAAAA??D?B7887736?A???A?@<<=>AEEAAAAAAAAAAB826-8739:694?@F@ABBAAB???BEABABAABAD?@?BAA?AA?=?<988:8?;;?>@?A865.;63797564889C@?>C5;BBA?AAA????A??@?A=@@@@ABCAA:====?D@?@AB????B?AAAABAAAAAAA?C??D@B>D<;804*5178:9@245188548AED@>?A8@?:@A@>?D???A@BB=<<<@;A<=?<:A?@@@?AB@@G??;9:1::9=:86/6?4792;;;EAAAAAAAAA?AAAEABAAAABBDAC1;1)62.72>??@82183*4+02)3737?;=D?=8+-7-5-162730;98:;2::/8:!*+!!'+342!5.3;???A@@;8983545:;;?AA@;6/78;=;=>C0/,9/3++*)(40-<<>AA??D@@@@?ACA@BBC@A7:!67.4,*3*+..3153,*(+-5+/),,,5.*)),,,40,+)*,,)**20-,,.+**)***-88-+2-+.-2<=32-*+)3-:.3+,/0)*,,-(,..*/.*)(/,..0/3/7/142+)))).,/582,*)()))-))//,))))++3)))))*1.0*')&+'+%''*22+)(((()+38,,))*)(()))((+()).()+*,&)*(&)&&),1)++/0,6.+,(&%,.5:15,*())(()+(+))))*,,),+,0325-'.12.,**,+,04,*&*+**-5@D1.*)+++.*)(,+)),''+''),-0-./946?<>>,))*,))+)0))()()),)),.5+,./,,4+.((''/,...()(,),((11592(.,)'))(,,*+11,**,,-)*+,1==A2/+5.-0;;:11*())','+.3/78;>??</*...1+*+(()( TN U13a03c03_1 QL 63 QR 627 AO 1 236 1 236 AO 238 608 237 607 AO 609 1043 609 1043 RT HAF3 63 253 = MIRA . RT HAF3 255 520 = MIRA . RT HAF5 521 547 = MIRA . RT HAF3 548 554 = MIRA . RT KMRF 554 574 = MIRA . RT HAF5 555 601 = MIRA . RT KMRF 582 602 = MIRA . RT HAF3 602 627 = MIRA . ER AT 1 565 63 627 RD U13a08f10.p2 RG 1 RS XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTAACAAATCATCAGTGGTTTAGAAATTTCATAAAATTTCTCATTTCAAAAACGAGAAAATTAAGGCCTTGCGGGGTTAAATCGTTATCAAGTAATCAGTCGGCTCACTGCCCAGTGTGGACACTGGCCAATATTATGGCACCAGCCTTTGAGAAAAGGAAACCTTTTTTTGAATCCATCTCCCTGACCTGGGGTTT*GGGTTGGGTTTTATCAGCATAGGGTGACAGGCAAGTTCAATCTGATTGGATCCTGGATCCCATCATGGGGTATCTGTTCCTAAATCATTCCCTGATCCTCGGTCAGAGCGAGCGCTTAGGTTCCCGCTGCAGTTAAACACCTGGTTCATCTGGGCATACTAAGGTTATGACTTTCCACCTGGGAGTCCACGACAACTGAAAAAACTCAAAACTTCTTTAAGTAGAAGTTGAGCCTGAAGCTGAGGCAGCTGTTTCAGAGAGGAGGAGGAGGAGGAGATAGCAAATGTCAAGGGATTTCCATTTCTCTTTCAATGCCAACATACTTCAGGGCATCAGCCTGGCTGTATCTGTAATCAAAAAACAGTTCTTCGCAAGTCTGGATGGCTCTCCTGACAAAAACACCTATCCTGTGATCACCATTAACCATCATAACTTTTGCATAGCAGTTTGGATTTACCGAATGATTTGCAAAACGAATTTTGTTACCCTTGCAGGTTGCATCCACCACAAAATCATTGTTCAAGTTGAACAGAAAGCTGCACATGTATTTATCACACACTTTCCCTCTTCCGTCAGTTTAGCTTCAGGAACCCCGAGTACTGTGGGAATTTAGAAAAGAAAATGCCGTTTGAAATTCTGAATTTGCAAAGTACTATTGAGATTATTTATCGCAATGAGTTTAAAACCCACT RQ !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!?;=;F@844AA4=@7AAAAAAAA@??@??B?<GA==4239==@@=:@:9;22;96:525:;896:;;;;??B;;AD??AAA?B???AAA??AA==>=?==@====?===>=??AAAAA?B;54/14=BACABBABA====@:=?<A=:CCABAA@@@@D??@@??ABBAAAA?A????AAAAAAAAAA??ECACB@@CGGEC@EEAAAA?A?:=>===AAAA?BAAA?AAA@======??>AAAB@8::==>F?>?DA?AAAAABBB???ACDFA@BAABB@>@>@DEEA@@?????AAAAAB<@;9=@A===;=AA=5====AAAAAAAAA???=@=>>?=;=???A?====??@C@>@@AAAAAAA??AA@====<C@A==8?==AAEA@D>@AG??:<61<<?>@@@@@@@AAAAAAAAAAAA?645/.5A=::87;?AB:=;::<AB??BBFBBBAABBEABFBBCG@@@A==BC;>D@?>??@8B?;331747?A=;==>E====@;A??E@?==8@=;;BA=>;=:9==44==;=??AA<:=9<6:???AAA;;841581/78;6;166:9?E?>@==9;98;9;=;92:/88====>==>?A:0575:;=====:EAA======AABAB62199==98===;544/6;6=4/3**35;5:;A9/()**0;0**,,-2;:?;;7-,+*-(,,,3555.*+-63;0+.,*(,+6892/.)*/),15?9-+))17=====@810(),-64-0/..**13*&(*,)(%)*--;3713-4.+(()),.,+/-+,*,,/389-'*)*(,)2A04,1./+,*/+)+)&&*.*)+)(()0/21**-.)((($(%)((++**+*-//(*())(((0(*(.*'%**))-++' TN U13a08f10_2 TS 1 QL 66 QR 816 AO 1 239 1 239 AO 241 931 240 930 RT HAF3 66 523 = MIRA . RT HAF5 524 550 = MIRA . RT HAF3 551 557 = MIRA . RT KMRF 557 577 = MIRA . RT HAF5 558 604 = MIRA . RT KMRF 585 605 = MIRA . RT HAF3 605 659 = MIRA . RT KMRF 659 679 = MIRA . RT HAF5 660 732 = MIRA . RT HAF3 733 733 = MIRA . RT HAF5 734 761 = MIRA . RT HAF4 762 773 = MIRA . RT HAF3 774 816 = MIRA . ER AT 1 751 66 816 RD U13a03e10.p2 RG 1 RS XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGCAAATTCAGAATTTCAAACGGCATTTTCTTTTTCTAAATTGCCCACAGTACTCGAGGTTCCTGAAGCTAAGCTGACGGAAGAGGGAAAGTGTGTGATAAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCTGCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAATGGTGATCACAGGATAGGTGTTTTTGTCAGGAGAGCCATCCAGACTTGCGAAGAACTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTTGGCATTGAAAGAGAAATGGAAATCCCTTGACATTTGCTATCTCCTCCTCCTCCTCCTCTCTGAAACAGCTGCCTCAGCTTCAGGCTCAACTTCTACTTAAAGAAGTTTTGAGTTTTTTCAGTTGTCGTGGACTCCCAGGTGGAAAGTCATAACCTTAGTATGCCCAGATGAACCAGGTGTTTAACTGCAGCGGGAACCTAAGCGCTCGCTCTGACCGAGGATCAGGGAATGATTTAGGAACAGATACCCCATGATGGGATCCAGGATCCAATCAGATTGAACTTGCCTGTCACCCTATGCTGATAAAACCCAACCCCAAACCCCAGGTCAGGGAGATGGATTCCAAAAAAGGTTTCCTTTTCTCAAAGGGCTGGTGCCATAAATATTGGGCCAGTGGTCCAACACTGGGCCAGTGAAGCCGACTGGATTACCTTTGATAAACGATTTAACCCCCGGAAAGGGCCTTAAATTTCCCCCCGTTTTTTGAAAATGGGGAAATTTTTTTAATGAAAATTTCTCAAAACCCCCTTGGAGGTTTTTGTTTAAAGCCCGTTTAAAAAAAAATTTCTCCTTGGTTAAAATAAAAGGAAGGGTTCTCTCCCGGGGGGAAAAT RQ !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!>9;8:533752?C544;4148449@7<@@@;=866686AB@=====@==E==;;;@=>===B==@::;;:7ABB99?A??<AAA>==;==?????AB??????AA??AAA==A========AA?B???@??A??ADCA@EE@@AAAAA======@??D@???BD?AAAAAA@=?C>:=?AAAAA??A===B???????D?@==<<@?AA?A???AC@BBAECAAA@@@C@@G@A@B<?A???AAAEE@@@BACA@@AACAFE=?7337AAAAEBA@?DD@AAACC?CD@ACAA@C@AABAABAAA=:===A<???A??AAA?AAA???AAAAAAC@@B@CAAAAABAA??BDA;7;?A?AACAABA@@CAAA?AA???<?87*.*29AAAAAAAABBBA@@?AAAAA?A==;BB??@?ABA??????@@>@@B?????@AA@AC?=<52*/38@A??>B=====8=>A=B======AB=8=;>==>==C=>=A=B=>=BA??A??DAAAA??===B=9EEAA?=;<538A??<773766C?@@EEBA?A>=6;/3=?4;8689C<79251:378=9:8:@BEAAA?AA=:5*,85=89..43:5:G@AAAABA>====>=>=====E=?AA??>3;:8=8=D?=?=?E;17?=?@=73/3/268B=B844*+()*))49BB==:3.,*1,**,**,-*2,/2,3*,5./;;8==>56.2@6A===7681/.33*+-66632,(,*).**.31,)(&))*)**()*00.56.4.1/1/6C85526575,,0,,41-+((.8882//35BB2/,+,*)'+*/54)(0)+,**&).))&),*'%''))***)*.*(/,))1*)))%(%'')(,-,**),0,3,+*,*)%,/3&&&)844..+))'$))*****050/++*+)**%+***))**+*(+..**))+')()& TN U13a03e10_2 TS 1 QL 47 QR 748 RT HAF5 47 67 = MIRA . RT KMRF 48 68 = MIRA . RT HAF3 68 73 = MIRA . RT KMRF 73 93 = MIRA . RT HAF5 74 119 = MIRA . RT KMRF 100 120 = MIRA . RT HAF3 120 162 = MIRA . RT HAF4 163 174 = MIRA . RT HAF5 175 202 = MIRA . RT HAF3 203 203 = MIRA . RT HAF5 204 276 = MIRA . RT KMRF 257 277 = MIRA . RT HAF3 277 331 = MIRA . RT KMRF 331 351 = MIRA . RT HAF5 332 378 = MIRA . RT KMRF 359 379 = MIRA . RT HAF3 379 385 = MIRA . RT HAF5 386 412 = MIRA . RT HAF3 413 722 = MIRA . RT HAF3 724 748 = MIRA . ER AT 824 123 47 748 RD U13a03e10.t1 RG 1 RS CCTTGCATGCCTTGCAGGTCAACTCTAGAGGACCCCTGCAAATTCAGAATTTCAAACGGCATTTTCTTTTTCTAAATTGCCCACAGTACTCGAGGTTCCTGAAGCTAAGCTGACGGAAGAGGGAAAGTGTGTGATAAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCTGCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAATGGTGATCACAGGATAGGTGTTTTTGTCAGGAGAGCCATCCAGACTTGCGAAGAACTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTTGGCATTGAAAGAGAAATGGAAATCCCTTGACATTTGCTATCTCCTCCTCCTCCTCCTCTCTGAAACAGCTGCCTCAGCTTCAGGCTCAACTTCTACTTAAAGAAGTTTTGAGTTTTTTCAGTTGTCGTGGACTCCCAGGTGGAAAGTCATAACCTTAGTATGCCCAGATGAACCAGGTGTTTAACTGCAGCGGGAACCTAAGCGCTCGCTCTGACCGAGGATCAGGGAATGATTTAGGAACAGATACCCCATGATGGGATCCAGGATCCAATCAGATTGAACTTGCCTGTCACCCTATGCTGATAAAACCCCAACCCCAAACCCCCAGGTGCAGGGGAGAAGGGATTCCAAAAAAAAGGGTTTCCCTTTTTCCTCCAAAAGGCCTGGGTGCCCATAAATTATTTGGGCCAAGTTGTTCCCACCACTTGGGGCCCGTTGGAACCCCGAACCTGAATTTAACTTTGAATTAAACGCAATTTTAACCCCCCCCCAAGAGACACCTT RQ (')*14.-*0-0**1,).0.%-.1:9479)7)'11>AAA=A>A>=912-/5:?A@DBA;;;@@@AAAA??ADAAEDBAAAAAABA@???@==40-:16:=???@?>9?C?;;C8?55-1.*1198<AA@F?;4686=?AAABA9;79;>DBABD@@A@C@AA=B=<><C8<=@;=@@@A@A??=C56>CC?CC?@A>87=;/<?A????A?AAA@@@A@@ABBAAA???@?AEAAAACC????BBB???AB@@D@>>?BC?@@F@@?==:;769?B69<570266=CBC==;;><?78-688@A??A<<942?:=;@85:49??DBAC@???D;A======ABA@?>?>6::=A<?DBB??;:;:;????<?CAAAA?C<;4;96====?AAACDCA@@AAAAAAAAA????BAAABAAAAAAAAAAAAAA==9===AAAAAAAAAAA@A88678B@AAAAAAAAAAA@A8==5;;?EAC9B=A?@@@B???=;;;?::===??8:2768=<@?9@?<??B???@G@@AAAAC@@;;;,2--81576A?>??A?A@A>=11/000210<=@@>5;75:ABB@@AA=7/6361,188@=====>8728709418823=5>1:-04128=:;;537753?<@?<4.8988;9;==<??@=;5;;@B==:=====9=757226.-7?C;/,///:?E;:)/.)((,()2/,,,',-/304)-337;?E=6242/72/5*,21,(((,,.243,)3-),1,))*/+)()+)*334:8/3')))*5,-+/5*+0)**,1**//2+1,(,,/-(,*-,1.,-,)))*.,,47/,0*+.))-**>+'*').,4371-*+22**-*-1))*)(+'&+*+& TN U13a03e10_1 QL 37 QR 680 RT HAF5 37 57 = MIRA . RT KMRF 38 58 = MIRA . RT HAF3 58 63 = MIRA . RT KMRF 63 83 = MIRA . RT HAF5 64 109 = MIRA . RT KMRF 90 110 = MIRA . RT HAF3 110 152 = MIRA . RT HAF4 153 164 = MIRA . RT HAF5 165 192 = MIRA . RT HAF3 193 193 = MIRA . RT HAF5 194 266 = MIRA . RT KMRF 247 267 = MIRA . RT HAF3 267 321 = MIRA . RT KMRF 321 341 = MIRA . RT HAF5 322 368 = MIRA . RT KMRF 349 369 = MIRA . RT HAF3 369 375 = MIRA . RT HAF5 376 402 = MIRA . RT HAF3 403 680 = MIRA . ER AT 824 181 37 680 RD U13a06d03.t1 RG 1 RS XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTTAAGTAGAAGTTGAGCCTGAAGCTGAGGCAGCTGTTTCAGAGAGGAGGAGGAGGAGGAGATAGCAAATGTCAAGGGATTTCCATTTCTCTTTCAATGCCAACATACTTCAGGGCATCAGCCTGGCTGTATCTGTAATCAAAAAACAGTTCTTCGCAAGTCTGGATGGCTCTCCTGACAAAAACACCTATCCTgTGATCACCATTAACCATCATAACTTTTGCATAGCATTTTGGATTTACCGAATGATTTGCAAAACGAATTTTGTTACCCTTGCAGGTTGCATCCACCACAAAATCATTGTTCAAGTTGAACAGAAAGCTGCACATGTATTTATCACACACTTTCCCTCTTCCGTCAGCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAAAAAAAGAAAATGCCGTTTGAAATTCTGAATTTGCAAAGTACTATAAGAATAATTTATAGCAATGAGTTTAAAACTCAACTTTTTATTGCCTTCTCACCAGCTGCAAAGTGTTTTGTACCAGTGAATTTTTGCaaTAATGCAGTATGGTATATTTTTCaACTTTGAATAAAGAATACTTGAAaTTGAAAAAAAAAATTGAGTCTGAtGGGTCTAGTGTGTTTATAAAATGACCTTAGGAACCCCCCGTTATTTTAAAGTTAGACCTTTACCTCCACTGGGTATTCCTTTTTGAAAGCCATTTATTTTATTTTTAAAAAAACCTAGTTTCTCCTTTGGCCGAAATGTTTTCTGAAATAAACCCTTTGAACCACTTTCTTTTGCCCTTTTTATTGTTAACCCGCCCGCCTTTTAACCCCACCGTTTAACACGGGAAACA RQ !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!@A???>??893;5.8;?B?C;=;====;88;=CAAA==961/9.5.5=7::>=8:=75:6/34-6;?A@??;<,6625777:5:4:C;?DAAAAAAAADAAACDB@@EAF@A>??EDBBFDGCAAAAF@DA@B?=AC?DAACCC@?@@AAEEEDAAA@@A;;>7==?=@==A=>==A?AAEB@??;???CC?32!*).66/8??AAABAC@EAAA=ACBAAB??AAA?97+799B@=?=A?@==B;=====?EA@@@?B@=B=A@A?A?AABAABB??A????BAA?B??=BB?::8:=9DD:=9:?5:838<@@?;??;AAACD?FACEA??AAACC@@CF?D?CA?CD?@A??;6??BBA>==6??A?B======>@<;4:=A:=====?<AA997+;????>???AAAAAA?6:=63*3?9>/86:??B?8??>?9A>=B16;9:=?>DA?D@A?C<6<=C==AAAAAA?BB89448=C=AAABD@?A???CAAAC@95778;?CABCA6.5--2+*3,495;></93!!.567?;++9-,0//23==DB33.!+4.66553?=>==51+*.()+.+!57;?AAAA@AA@@8/1((21/32!*51345514*)(21./3/00+/,-))),-*,0))11:87?6941355)),'*)))'*,(*()-*),)+-*,+)((+,+1.::42+.-(+.,6*-+(*+53-.3.,.(*(+)6.+)**,**)(,,2*+5+(*)).*'0**1+*(*,+-2-01/)*)))-+)))(),()*,).,2)+,.03.+)$')'-2*..-)*+)()-+0056))*-6+))))))))/..(++*-))&& TN U13a06d03_1 QL 33 QR 662 AO 1 588 1 588 AO 590 867 589 866 RT HAF3 33 100 = MIRA . RT HAF5 101 127 = MIRA . RT HAF3 128 134 = MIRA . RT KMRF 134 154 = MIRA . RT HAF5 135 181 = MIRA . RT KMRF 162 182 = MIRA . RT HAF3 182 236 = MIRA . RT KMRF 236 256 = MIRA . RT HAF5 237 262 = MIRA . RT HAF3 263 263 = MIRA . RT HAF5 264 309 = MIRA . RT HAF3 310 310 = MIRA . RT HAF5 311 338 = MIRA . RT HAF4 339 350 = MIRA . RT HAF3 351 393 = MIRA . RT KMRF 393 413 = MIRA . RT HAF5 394 430 = MIRA . RT HAF3 431 445 = MIRA . RT KMRF 445 474 = MIRA . RT HAF5 446 473 = MIRA . RT HAF3 474 505 = MIRA . RT HAF5 506 580 = MIRA . RT KMRF 561 613 = MIRA . RT HAF3 581 581 = MIRA . RT HAF5 582 610 = MIRA . RT HAF4 611 611 = MIRA . RT HAF3 612 662 = MIRA . ER AT 391 1020 33 662 RD U13e07f02.t2 RG 1 RS ACATGCTGCAGTCGATCTAGAGGATCCCATGTCAAGGGATTTCCATTTCTCTTTCAATGCCAACATACTTCAGGGCATCAGCCTGGCTGTATCTGTAATCAAAAAACAGTTCTTCGCAAGTCTGGATGGCTCTCCTGACAAAAACACCTATCCTGTGATCACCATTAACCATCATAACTTTTGCATAGCAGTTTGGATTTACCGAATGATTTGCAAAACGAATTTTGTTACCCTTGCAGGTTGCATCCACCACAAAATCATTGTTCAAGTTGAACAGAAAGCTGCACATGTATTTATCACACACTTTCCCTCTTCCGTCAGCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAAAAAAAGAAAATGCCGTTTGAAATTCTGAATTTGCAAAGTACTATAAGAATAATTTATAGCAATGAGTTTAAAACTCaACTTTTTATTGCCTTCTCACCAGCTGCAAAGTGTTTTGTACCAGTGAATTTTTGCAAtAATGCAGTATGGTaTATTTTTCAACTTTGAATAAAGAATACTTGTAAATTGAAAAAAAAAATTGGAGTTCTGGATGGGTCTACGTGGTGTTTATAAAATAACCTAACGCATCGCCCCATCCAGCTTTTTTTCTGAGAGAGTACAGATACCAGCCACTCCCGCGGGGTTGTTTCGATCTTGCAATGCCCCGCCAACTTTTTAATTTTTAGCAGAAAACCCCAGGTTCTAGCTCCCATTGACCAGAAAAATTGTTTATCCTCGGAAATACAAACGCCCTGTTGGAACCACAACTCATGCCTCTCTGCGCCCCTCAAGTTCGTGTAAATGTGAAAACCAGCGCCCCGCCCCGAGTTTTAGAAACGCCCGCCCCGCCTTGTGGAAGCCTTAGGGGAAGTATAACGCCCAGCGACGTTCTGCTCTCCTGGACAGCGTCGGCGTATGGTATCTGACGGTCTAAACATACGTCACGTAACGGGAGCCCCCCTCTTCCGCATCTCTCCCCACTATTTGTGGCTAGCAGAAGAGAAATAATAAGCCGCAGCGTATCTGATGAGGAGCAGACGTTATCACGCTCTGCGGTGGCGGTCTTTAGAACACAAAATTTCGCTGTTGTCGCGCCCTTCTCCGACGGAAAAGAGAAAAAACCCTCTCTGTCTTTTCTATTAAAATAAGTAATTGGTGGGTTTTTTCTGCTGCCTTCTGACGACGACCGAAGAGAAACGCGGGTTTTGTATAACGCAGATATCACATCGCGCGCCCTCCGCGTCCG RQ *'-,,*55(,++/)''..912.-')).3ABAABAA=02-633===ABBAAA?A?A@AECAAAABADAA<>225*5/.6=?AADDAAAAABB???C?AAAAAEE?AA?A@AAAA??=@;:;77:9=B=>AABCA??B??A?AAAAAAAAAACCA=8<;C==@@@@A?AA?A?AAAAADAAAAADA::>67:5A==????=?9965?>9>?>>ADAAAB39*776??DC?<<?A<??88;69;>;A59A@?@@AAAAA@A?=AC@AC@8A18094130.9525;7;>;=77>>5;@@?=A8:?@>>==A;??>>?@@A<;<7;9-*1(//,0577;231.,+828=92;;@@;?@?A84:197138.705AA@@A@@88889....02;?@>=:?@99>A@@AD=6:6=7395577A;=B84*-)2/8FB@@@<13.!/8=@@??;98181215<<;:=9/.***(++*+),*/2571-65*7/8944/3,0*&+!),28;2*0*.000!/,.7>=>5.1.1389B83;.3.2,(2-())&,)01/.318=84-,**((+)/)22-**,.*+2-+(()(,,**.2659.+-+),,%-)))*())(((,,,)(,,+**)(()*(**)+%*()&*'')'')+)))(+**))')(),*)&+%)-,*-)**,.))**+,,*-.*)-,,)(((()(()*-++*)*-**,)*((((),-,))(()('))((())**)*)+)*+*,.22-),+*++*++**,)*..2-)(+***+&('(+&((()+**,*,)***))())))+,(,.((,*-.***(*)((''('('(*(*)))*,*(%$)(()((().3+(((*-**'%)))+++)+*,*)*+--**)*,(*,&&)%%+-1,+*-)+*''('(')*))()+)++))'())))))*)))*-,*,,)))))(*)(((-)***+,*)(*+-*)&()''*.).('&)'*)-))()++30*+**%***(,))),))-,0))(++***((())*1,-64/2,./.-/()).*)))))(('')++).++,))--))-)6'(''''(-,//,/)%)((+((''***()/*())*+*0'+)*+**),*))+'((&(+**,-+*)+-:;.,*,**)+()'+2-192-*+(+(*$$+$)'))0+,*,2*)-).+'+''*)(+*,))*&*++')(++(+(()+))($(&(*+().'*+)))+)'-))+)&*))))**))*)()*+)*,+))))'+)+/12,)(')+$'*()(* TN U13e07f02_2 QL 29 QR 538 RT HAF5 29 55 = MIRA . RT HAF3 56 62 = MIRA . RT KMRF 62 82 = MIRA . RT HAF5 63 109 = MIRA . RT KMRF 90 110 = MIRA . RT HAF3 110 164 = MIRA . RT KMRF 164 184 = MIRA . RT HAF5 165 237 = MIRA . RT HAF3 238 238 = MIRA . RT HAF5 239 266 = MIRA . RT HAF4 267 278 = MIRA . RT HAF3 279 321 = MIRA . RT KMRF 321 341 = MIRA . RT HAF5 322 358 = MIRA . RT HAF3 359 373 = MIRA . RT KMRF 373 402 = MIRA . RT HAF5 374 401 = MIRA . RT HAF3 402 433 = MIRA . RT HAF5 434 508 = MIRA . RT KMRF 489 529 = MIRA . RT HAF3 509 509 = MIRA . RT HAF5 510 538 = MIRA . ER AT 459 968 29 538 RD U13a05b10.t1 RG 1 RS ACTTCCCTTGCCGCTGCAGGTCGACCTAACAGGATCCCTGGCTGTATCTGTAATCAAAAAACAGTTCTTCGCAAGTCTGGATGGCTCTCCTGACAAAAACACCTATCCTGTGATCACCATTAACCATCATAACTTTTGCATAGCAGTTTGGATTTACCGAATGATTTGCAAAACGAATTTTGTTACCCTTGCAGGTTGCATCCACCACAAAATCATTGTTCAAGTTGAACAGAAAGCTGCACATGTATTTATCACACACTTTCCCTCTTCCGTCAGCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAGAAAAAGAAAATGCCGTTTGAAATTCTGAATTTGCAAAGTACTATAAGAATAATTTATAGCAAGGAGTTTAAAACTCAACTTTTTATTGCCTTCTCACCaGCTGCAAAGTGTTTTGTACCAgTGAATTTTTGCAATAATGCAGTATGGTATATTTTTCAACTTTGAaTAAAGAATACTTGAAAtTGAAAAAAAAAATTGAGTCtGATGGGTCTAGTGTGTTTATAAAATGACTTAAGATCCCACAGTTATTTTAAAGTAATACATTACACCACTGGTATTCATTTTGATGCCATTTTTTTATTTTAAAAAACTAGTCATCCTTGCAGAAGTGTATCTGATTAACTCTTGACAACTACTTTGCCATATTTTATTACCAGCTGCATTTCCCCCCGTGACAGGATAACTAATTTCTATTGTTTTTGTATTTTTGCCGCCACCTTCCAAGATAGCCCTAGGAGTACTGTGTGAAATCCTTGCATCAGAAAGACCTGCTTTAAAATAGTCATAAAGAAAGTAAGAATACATTCAAAAACTCCTTT RQ (+/+*&)))+)))+)+10/79:*-))*.*(.,.***-6====A=>@E==A=>87;:BC<863)*;:A@?=7=CA??:746;BC?????;@=>=A>A993578@@=A?AAA==::=A=@@==?B=>=AAEAAAAB@>?AA?@FAC@BD?;;;??=@@===D==::4-6,25<=8;2<79@@B?;6;>==?984106<>??=0.+*/-/67AB@;5*.37:9==@?=.00*3.5;;;?@@;>4:==????CA;;.,,*-<2,+2,.2245===?A?9=?;>A6>6/669==B=?=<6853*708;873>39?.*)(1-2==:A9=:=?A@@=9<@@<9????>@@AA?;@5<?9@7:9=::305.32.2.95;?A5-))2,*729**608??23???CAC98+.8;>A?@@@-2!)23.664<AAA?:2.>>C@:4!.0465=<12;>56;4*+,3/*C;8@A???>><=CBA:8://01.!,(*<1823...167,,!(21=28677551137943,!)2.2-0>??+/;16;;GAA=1-,+.:<6964626497?7:0084;9AC?AAAAA;;85>-53-1+77?:?;@C;???@7898;;=6::***,)*0/5+.463/.6774,1-8:?@AA=@;A-*4.84.37.,0).1(1;9@</-*,.)**,/5*-663-3-042,,23-,,.024,2::20/,-38/1/34355-1+).++)*//-*2-2+2,-46:>;:,(1**314,+,+)25,*))-+*/--**((+(0//*---*(*++)01--1*,(()(),-?311'+.))'(()17:6-++(+*%,19-(**)*-&'+*..(+**((,*,0))+)()0/ TN U13a05b10_1 QL 37 QR 676 AO 1 373 1 373 AO 374 437 375 438 AO 438 478 440 480 AO 479 480 482 483 AO 481 504 485 508 AO 505 539 510 544 AO 540 853 546 859 RT HAF5 37 64 = MIRA . RT KMRF 45 65 = MIRA . RT HAF3 65 119 = MIRA . RT KMRF 119 139 = MIRA . RT HAF5 120 192 = MIRA . RT HAF3 193 193 = MIRA . RT HAF5 194 221 = MIRA . RT HAF4 222 233 = MIRA . RT HAF3 234 276 = MIRA . RT KMRF 276 296 = MIRA . RT HAF5 277 322 = MIRA . RT KMRF 303 323 = MIRA . RT HAF3 323 328 = MIRA . RT KMRF 328 357 = MIRA . RT HAF5 329 356 = MIRA . RT HAF3 357 388 = MIRA . RT R454 372 373 = MIRA . RT HAF5 389 463 = MIRA . RT R454 436 437 = MIRA . RT KMRF 444 496 = MIRA . RT HAF3 464 464 = MIRA . RT HAF5 465 493 = MIRA . RT R454 477 478 = MIRA . RT HAF4 494 494 = MIRA . RT HAF3 495 581 = MIRA . RT HAF3 585 608 = MIRA . RT HAF3 621 676 = MIRA . ER AT 512 1151 37 676 RD U13a04c06.t1 RG 1 RS XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATTTTAAACAACCTCTTCTGAAGCAAGATTTAACACAGTACTCTACTGCTATCTTGGAAGGAGGAGGCAAAAATACAAAAACAATAGAAATTAGTTATCCTGTCACGTGGGTTAAATGCAGCTGTTAATAAAATAGGCAAAGTAGTTGTCAAGAGTTAATCAGATACACTTCTGCAAGGATGACTAGTTTTATAAAATAAAATAATGGCATCAAAATGAATACCAGTTGAGTAATGTATTACTTTAAAATAACTGTGGGATCTTAAGTCATTTtATAAACACACTAGACCCATCAGACTCAATTTTTTTTTTCAATTTCAAGTATTCTTTATTCAAAGTTGAAAAATATACCATACTGCATTATTGCAAAAATTCACTGGTACAAAACACTTTGCAGCTGGTGAGAAGGCCATAAAAAGTTGAGTTTTAAACTCATTGCTATAAATTATTCTTATAGTACTTTGCAAATTCAGAATTTCAAACGGCATTTTCTTTTTCTAAATTGCCCACAGTACTCGAGGTTCCTGAAGCTAAGCTGACGGAAGAGGGAAAGTGTGTGATAAATaCATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCTGCA*GGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCaAACTGCTATGCAAAAgTTATGATGGTTAATGGTGATCACAGGATAGGTGTTTTTGTCAGGAGAACCCCCCCAGAATTGCGAAAGAAACTGTTTTTTTTGAATTACCGAATACCAGCCCAGGGCCGAATGCCCATGAAAATTATGTTTGGGCCTTTGAAAAAGAAAAAAATGGGGAAAATCCCCTTTGGACCATTTT RQ !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!@@=B@:6;2@=,?647<>>ADB??:0,1..53392=A2711?AAABAAAAAAAAAB==;667.88,367;=9===867:AAB=A52)..0;;?@CC@???>AAC>@9@;;9>4:>@=8??@;?>>9>@BA??;;;9@;AD@8;5,75<@9/2*777<>A<4,*736AAAB=;;9;86:4:7187ABCDD?=?/*8;AAA:=:@859:?>AAA=B??@@@@@A@??49:5;=C<@=?????>???AAAAD=9>?;;?@==;;6586@8@???34!---58>@@B?:7-837::3+/29;;?C:9.977??7:;;?@>@@AAB?@??>?AA@B684-8@@@?@DAAA>>,*0;?>AB>B>A>A??86@@@B?@=???CGAAC@@@@EBB75;;=;:8415138=?D=78,/.*32:B>>@?;8888C@@@@@A????<6;>9;C=@?87:9:?ADD;74;<AAAAA@@>AB?>@EBBB@A@AAA@;CA=C?7;578?@@@@=@@@@A:;4+486<7/--/2/.5/49?AAA;>7)99?>::7/41./1.75C?<AAAA@@A=8>=0.!*-9BBAAADE@AAAAAA?????9;;;2;8==:<6=A?==:C<677=;==DA?1./*,.1=:,024*,/285CCAA@@65543-)(/-/4=7/,).1!/,,-2)(.().+48.!1**031;8==?<=002-*-./.71.976/.;;;BAB?<7-()(+,)+)+.+()12:*0*(2+,*-0156522/111;5;;=7//,)*(,*,/-+*+1'+09<0),)*()1-(('(,'++10.+61/3--+)+/./,*-*.376588773?:88*-11::515,20,,-+*(()(-+33A< TN U13a04c06_1 QL 51 QR 764 AO 1 660 1 660 AO 662 897 661 896 RT HAF3 51 355 = MIRA . RT KMRF 354 406 = MIRA . RT HAF4 356 356 = MIRA . RT HAF5 357 385 = MIRA . RT HAF3 386 386 = MIRA . RT HAF5 387 448 = MIRA . RT HAF3 449 493 = MIRA . RT KMRF 493 522 = MIRA . RT HAF5 494 521 = MIRA . RT HAF3 522 527 = MIRA . RT KMRF 527 547 = MIRA . RT HAF5 528 573 = MIRA . RT KMRF 554 574 = MIRA . RT HAF3 574 616 = MIRA . RT HAF4 617 628 = MIRA . RT HAF5 629 656 = MIRA . RT HAF3 657 659 = MIRA . RT HAF5 660 730 = MIRA . RT KMRF 711 731 = MIRA . RT HAF3 731 764 = MIRA . ER AT 1274 561 51 764 RD U13a08f11.t1 RG 1 RS GGGAGTGCACTCGATCTAGAGGACCCTATTTATCACACACTTTCCCTCTTCCGTCAGCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAGAAAAAGAAAATGCCGTTTGAAATTCTGAATTTGCAAAGTACTATAAGAATAATTTATAGCAAGGAGTTTAAAACTCAACTTTTTATTGCCTTCTCACCAGCTGCAAAGTGTTTTGTACCAGTGAATTTTTGCAATTAATGCAGGTATGGTTATATTTTTTCAACCTTTGAATCAAAGGAATACTTTGCAAATCTGGAAACAATAACTATACTTTAAGTCTGAAACTCTGTTCCTGGATGTCGTTTTAATAAAAATATGGACCTTCAACGAATCCCCCACATAGCTTTAATTTTTAAGAAGTTTAAGATACAACATCTTCGACCTTCAAAACTGGGTTAGATATCTCTATTCTTTGGATGGCCCACTTATATTGTCTCTCAGATCTTCTTCTATTACATAACTAATAGCGTCCGGTTCTCACTCTCTCTCTCGACCAGCGAAGGTATGCTGTGCCCTGGAATTGAATACCTCCTTTCGGAACACTACACTGCTACTTTATGGCCCTTATACTTATATAATCTTATATCACTATGAGCCTGGCCATATCTTACACCGCGCGCAGACGGTTGAGAACAGACGATAAGACCACTACACATCTATTCTCATAGATTTGTTTCTATCTGAGTATGATATCTTCTCTGAGCCTCTCCACTCTCCCTTCTCCGAATGAGGCAATATAGCGCACGATGCTCAGAGAAGCGTAGTCGCGTGGTTGGTTTCTCGATAGATCCTCTTCGCCCTCTCTCATGACAAAAGCGAAGCACTCTTGTTCTCTGAACGAGAGTCTAGCTGTCTTTACCAGAGACGGAAATGAGTTGTACTAAATTATACTCTACTCTCGTCCATAAGCATCAGCCGCGCCGTCTCTTATCGCACATCTCTGCTCTCTCAATAGCTATCGTGCATGTATACACAGAGAATAGATCATGCATGTACTCGTATATACGTTATGTCATCACTCTCTCTGCGTAACACATGACGATATCTGCTGATCTGCGCGATCATCTCTCTATTCATATATCATGATAGGGTCGTCTGTGAACACATCACATACTCATCTTATCGCGAGAA RQ ''$&'&-&((,/,')68;=2.+&(*.1=:9==9:@@?A?9::=::=BAA???AAAA@@=>==AAC?=>:489/-1(22/===BAAG@????<7;397498/730????A=D??@;<@?>=A8?884/37>@AAC=A::2.2.744??:>;982:A8)'03/97=@?<@@?@A@B@@?::>:;;@=B;95;76886==@C=:?8?:A;5;:7/,/-*20233623-<:6*))*)),,*-*+74596.2//5/.*7:32,/,/2/<,+*)*+/-*2/,-2*-2+--**)+**,-,*++++)()+)())))(,,()(((,,)*),))+)&()))*+'+())&**,3//5+)*((+)*())'((++%()2+:@/-,***(.*,+./)**,.6-))*+***./*,**-1&**((*())+)(,+)*+)*-*+*+/,-.((()(512,)),.,+,*(*+*+/5),'')'0+)()((((..3))*'(()*-,*0(+/+(*))*(()))-))*,'((*,/*))')))*++,*,*()(,(()+((+*,,**)*),&()*.,--3+(+,))(*(+/(,,)*()())((,)+.,)),'''1)+*(*)*0/.-*)+),)(('//(*-+1..*0*.-.+/-4+*))))*/.)+++*),,)))+))+('+))))(()*)))-+4''))''()))))-/0.0+.81/*+*,*+)+,)/,1,,*/,))+,***-)))))+)()))))-0,,+)))('-)*(*++)-62,*)))()++))+-')**.,./)+*()+)))))))()***11.1))''+()+)1)),,))*,,,)*15.*+1***+)+)).&****,,.3*/.-+)),/-**))),)))(+**))+),0*)(($*)()))%.+*+/*)*,+*&)')*0()+()+*)-)(()))))'()'''(()2/,.))-/+-,1+(-'+'')+)))(()),,*)))).)/--)()(-+)()))*,+3-0..-*+()*.++')++')')))%**.6456**,*+))(,(..713+0*,.1))(,)+*)(1())))*,.)*0,)++,+/76,,,,))-)+)+,),(),***,)(*.,,.:,+*+1(((,,)**%,*,)))+*)*5/:/0+(((&))&()))())+*)))(***.02*,())),()*(++*+)))(' TN U13a08f11_1 QL 27 QR 230 RT HAF3 27 57 = MIRA . RT KMRF 57 77 = MIRA . RT HAF5 58 103 = MIRA . RT KMRF 84 104 = MIRA . RT HAF3 104 109 = MIRA . RT KMRF 109 138 = MIRA . RT HAF5 110 137 = MIRA . RT HAF3 138 169 = MIRA . RT HAF5 170 230 = MIRA . ER AT 721 924 27 230 RD U13e07d02.t2 RG 1 RS XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATTTTATACTTTTAGGCACTACTGGTCAAAGTATTTGGGATCTTTTCCAGAATTAACAGTCAAGGT*CCATTTGTTGTCTTGGAATATGTACTTAA*TTATATTAAATTTTAATTTTTGTTTGAGTAATGTTATACTGGTAAAGATTTTTAAAATTGATACTCAGAGCCCATCTTTTCATGTTTTTGTTCAGACCTTATATGTATGCATAATTTATTCAGAGTAGACATCTTTTTTCTTTTTTAATTTAAAATTATAACGTGTTTTTCTATGTATTTTACTTTCTTTATAACTATTTTAAACAAGCTCTTCTGAAGCAAGATTTAACACAGTACTCTACTGCTATCTTGGAAGGAGGAGGCAAAAATACAAAAACAATAGAAATTAGTTATCCTGTCACGTGGGTTAAATGCAGCTGTTAATAAAATAGGCAAAGTAGTTGTCAAGAGTTAATCAGATACACTTCTGCAAGGATGACTAGTTTTATAAAATAAAATAATGGCATCAAAATGAATACCAGTTGAGTAATGTATTACTTTAAAATAACTGTGGGATCTTAAGTCATTTTATAAACACACTAGACCCATCAGACTCAATTTTTTTTTTCAATTTCAAGTATTCTTTATTCaAAGTTGAAAAATATaCCATACTGCATTATTGCAAAAATTCACTGGTACAAAACACTTTGCAGCtGGTGAGAAGGCCATAAAAAgTTGAGTTTTAAACTCATTGCTATAAATTATTCTTATAGTaCTTTGCAAaTTCagAATTTCAAACGGCaTTTTCTTTTTCTAAATTGCCCACCGTTTCCCGAAGGTTCCTGAAACCAAACTGAACGGAAGAAGGGAAAGTGTGTGAATAACTC RQ !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!=9=;49.-,,66,2(*18=56--1A;CEC?4787<?A;;;A:=BBAA=@@992;A>@D?EAEB894/+75??BD?AAAAA??@=B=@?A??B???AABBEAAAABA@@@?CCFBBBAC@A>???>;AA@ACA?;8817AA?=;8;58?DDBAAAC???>CC93.*9.8CAAABAEDA@>D????A@??5:94>?:@BAA=??A?CDA@CCAB@AADC??97176/6;1?ADABAAAAAADAAAAEAAAAAAAAAAAAD??@??@DDAAA??8===?AAAAAAAADAAABABBBCAADAADDFAD@?CADDD?=A<5;2686==AAAC@AA@DDFCAFD??;;:8AA=>@:=9:==>>=88:+363?=;?@@EDFAAAAA?AA?AA??AAA===C?;<?@??=844.97?A@?:9A97B;;=>>AD9::646+6>2>806669>@??;8@???@=<<;@;E@D@AABAD>=>>=@===AAAEFFAAAAAAA??59?=@?20)),.7<@DC>==9=>A@;6532748?@@;<5<==A;==8==61**0-12173>@<@=A@2935<EBAA??AA??B>81..2988:61*25:<B@CCAADDEAAA??B?AA61-+34/836ADAA@98!8:55-397BD:1+,!.2,.**/+--0?<8-,2*-24=;;5?>/5+,*24.,.+(+))*(-*(,!)-(-*(9,,,,(*.6@AA>!/-/(()+23;67>61)()(*()****,5:>A8;-,-*()!))))),-,!+*,!!5*43,+)))*-3+!,65,*++,/-((+,.36-4,+)+((0,())+&3*.)*++**)/4(5/(3..*)-/0453315702**-*14*-+/+(+.*((++ TN U13e07d02_2 QL 126 QR 882 AO 1 145 1 145 AO 147 175 146 174 AO 177 721 175 719 AO 723 773 720 770 AO 774 797 772 795 AO 798 833 797 832 AO 834 836 834 836 AO 837 855 838 856 AO 856 869 858 871 AO 870 943 873 946 RT HAF3 153 689 = MIRA . RT KMRF 688 740 = MIRA . RT HAF4 690 690 = MIRA . RT HAF5 691 719 = MIRA . RT HAF3 720 720 = MIRA . RT HAF5 721 782 = MIRA . RT R454 773 774 = MIRA . RT HAF3 783 827 = MIRA . RT KMRF 827 856 = MIRA . RT HAF5 828 855 = MIRA . RT HAF3 856 861 = MIRA . RT R454 857 858 = MIRA . RT KMRF 861 881 = MIRA . RT HAF5 862 882 = MIRA . RT CRMr 870 870 = MIRA . ER AT 1533 777 126 882 RD U13a03g02.t1 RG 1 RS XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTGCAAAGTACTATAAGAATAATTTATAGCAATGAGTTTAAAACTCAACTTTTTATTGCCTTCTCACCAGCTGCAAAGTGTTTTGTACCAGTGAATTTTTGCAATAATGCAGTATGGTATATTTTTCAACTTTGAATAAAGAATACTTGAAATTGAAAAAAAAAATTGAGTCTGATGGGTCTAGTGTGTTTATAAAATGACTTAAGATCCCACAGTTATTTTAAAGTAATACATTACTCAACTGGTATTCATTTTGATGCCATTATTTTATTTTATAAAACTAGTCATCCTTGCAGAAGTGTATCTGATTAACTCTTGACAACTACTTTGCCTATTTTATTAACAGCTGCATTTAACCCACGTGACAGGATAACTAATTTCTATTGTTTTTGTATTTTTGCCTCCTCCTTCCAAGATAGCAGTAGAGTACTGTGTTAAATCTTGCTTCAGAAGAGCTTGTTTAAAATAGTTATAAAGAAAGTAAAATACATAGAAAAACACGTTATAATTTTAAATTAAAAAAGAAAAAAGATGTCTACTCTGAATAAATTATGCATACATATAAGGTCTGAACAAAAACATGAAAAGATGGGCTCTGAGTATCAATTTTAAAAATCTTTACCAGTATAACATTACTCAAACAAAAATTAAAATTTAATATAATTTAAGTACATATTCCAAGACAACCAATGGAACCTTGACTGTTAATTCTGGGAAAAGATCCC RQ !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!DEEEAA=2?777;42;/73E;;=@=@@?@AA>==>=;?BB@@@AA?ABAAABAAAA@AEABAB?A==?@AEEBAAAAADBECCA========?7=3==AABDEEEEDADFA????DB?AAAAAAAE@=@==@@@@=BB==:@@>E?A?AAA?@@A=AAEFBEFE@?==@>;AA???;B==??A?A????AFCABCCDC?A?DAA@????DAA@DAAAAABABEEAAAABAAAA@@CD@BC@ED@A?>?@CBA?B=>>:@EEAAAACAAAAAABBAAABACD?AAA?????@A???8:=5:6;A@DA?AAAECAAA==>=:9ADABC@@==>>DAAAAABABAEEEAAAAABEDA?A:@=<@>A?===8==?ACEAAAAAABAA<<??<?>>;;;ABAAAABA>@@AAAAEEE?AA;=>EAAAD?8A39:<==?????CCDAAB@@=:<=>880424?DADEBAAAEEEEBAABA??AAA?;?9??>@==8@ABBA=AA?@9:C;C;AA@DABCC@ADBEAAABAABBA@=B;;>????;=8<>>=@=AAAA@A><0.2-*30:82/)/,'*11=::A=@;42+99BD56-./(/+-BD@@=:==842?3/*,39=A??<<6.8)053;9AAAA??B?:611-*211*,3-0**.03;A4..56@B;:60.).)+)-.42,*(,*0)*&*1--1+-72054-+02?8:-,0.+,9(+22..'(,10 TN U13a03g02_1 QL 44 QR 757 RT HAF3 44 87 = MIRA . RT HAF5 88 162 = MIRA . RT KMRF 143 195 = MIRA . RT HAF3 163 163 = MIRA . RT HAF5 164 192 = MIRA . RT HAF4 193 193 = MIRA . RT HAF3 194 730 = MIRA . ER AT 820 1533 44 757 // EC CO MIRA_rep_c2 NR 6 LC 543 CT MIRA 1 5 = . Note=Assembled with MIRA CT IUPc 22 22 = . Note=Strain 'StrainX': W CT H454 32 32 = . Note=No edit. PL: 1 RS: 2 RL: 0 RWTS: 0 EL: 0 CT IUPc 42 42 = . Note=Strain 'StrainX': Y CT IUPc 173 173 = . Note=Strain 'StrainX': K CT IUPc 209 209 = . Note=Strain 'StrainX': R CT H454 431 431 = . Note=No edit. PL: 3 RS: 4 RL: 0 RWTS: 0 EL: 0 CT H454 444 444 = . Note=No edit. PL: 5 RS: 4 RL: 0 RWTS: 0 EL: 0 CT H454 455 455 = . Note=No edit. PL: 2 RS: 4 RL: 0 RWTS: 0 EL: 0 CT DGPc 464 464 = . Note=G: 3 *: 2 - 40%25 CT H454 464 464 = . Note=No edit. PL: 1 RS: 3 RL: 0 RWTS: 0 EL: 0 CT DGPc 469 469 = . Note=A: 3 *: 2 - 40%25 CT H454 469 469 = . Note=No edit. PL: 3 RS: 3 RL: 0 RWTS: 0 EL: 0 CT DGPc 475 475 = . Note=T: 3 *: 2 - 40%25 CT H454 475 475 = . Note=No edit. PL: 4 RS: 3 RL: 0 RWTS: 0 EL: 0 CT DGPc 480 480 = . Note=T: 3 *: 2 - 40%25 CT H454 480 480 = . Note=No edit. PL: 1 RS: 3 RL: 1 RWTS: 0 EL: 1 CT IUPc 481 481 = . Note=Strain 'StrainX': W CT H454 490 490 = . Note=No edit. PL: 4 RS: 3 RL: 1 RWTS: 0 EL: 1 CT H454 506 506 = . Note=No edit. PL: 1 RS: 2 RL: 0 RWTS: 0 EL: 0 CT DGPc 520 520 = . Note=*: 1 C: 1 - 50%25 CT H454 520 520 = . Note=No edit. PL: 2 RS: 1 RL: 0 RWTS: 0 EL: 0 CS gcaatatcgtgacgatatgtawgataaatacatgtgcagctytctgttcaacttgaacaatgattttgtggtggatgcaacccgcaagggtaacaaaattcgttttgcaaatcattcggtaaatccaaactgctatgcaaaagttatgatggttaacggtgatcacaggatakgtatttttgccaagagagccatccagactggcgaaragctgttttttgattacagatacagccaggctgatgccctgaagtatgtcggcatcgaaagagaaatggaaatcccttgacatctgctacctcctccccctcctctgaaacagctgccttagcttcaggaacctcgagtactgtgggcaatttaaaaaaagaacatgcagtttgaaattctgaatttgcaaagtactgtaagaataatttatagtaatgagtttaaaaatcaactttttattgccttctcaccagctgcaaagtgttttgtwccagtgaa*ttttgcaataatgcagtatggtacatttt*cactttgaataaagaatacttga CQ +&-,(,)+))*(/)0*,120--3??@CC@@@5??@GED:>5,;BFGFGABCC@5???>C@DGF??EHEHDGEHEDACD@ABFDE<67:9>CBBCGEGEBBGIJEHDEHDIFHHHDBDEEDEIIHDEIDHHDEEEDDFGEFEEFFDEGB@?@GADDDDHCCGDBEIF?<E83/*878GGIFFHEEC::=23<CGDEA>;:==<GDFB=<+7/;FHHIIHFJGEFAEB>ACGCHIEDDEJJDDFCCEDGDDFC=2@??=>>6>?DEBDC>;040?CCCCEDHEIDFEDGFAAFEEJHDDDDEEHHEEEEEDFEDHGEHAEFCDEHIGIHGFHHEE@?=69979;=EAD3:/0::?CA?DCEDD??CDDCHG>C?@CBDDDGDDFFBAFFDDG??@AEDCCAHDDDDDDFE><9?=:GDDEDEDHDEDC>A<79C?ACHHDG?DED+HHFDDDDCBB9@B??C?<?1CBB>.CB>??+?HB;9<(5)/C8@<)?HB7=CD@:BHB?>3,>66777BBC;<G5343@7G5<76:47400,-.62++, \\ RD 230375b.t7 RG 1 RS ACGAGCAATATCGTGACGATATGTAAGATAAATACATGTGCAGCTCTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCCGCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAACGGTGATCACAGGATAGGTATTTTTGCCAAgAGTGCCATCCAgACTGGCGAAAAGCTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTCGGCATCGAAAGAGAAATGGAAATCCCTTGACATCTGCTACCTCCTCCCCCTCCTCTGAAACAGCTGCCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAAAAAAAGAACATGCAGTTTGAAATTCTGAATTTGCAAAGTACTGTAAGAATAATTTATAGTAATGAGTTTAAAAATCCACCTTTTAATGCCTTCCCACCACGCTGCAAAGTTTTGTTGTTCCACTGAAATTTTTGCCATAAATGCCGTAATGTTCATTTTTCCCCCTTGAAAAAAAAGAACTTTGAACTTGGAGCCGGTCGCCGTGGCTTAAGATTTTTTTTTAACCGGCCGCGAAGCCTTATTCCCCTTTAATTGAGGGGGTTTATTTTTAAACTTGGGCCCTTGGCCCCCCCTTTTTACAAACCTTCTTAAATTGGGAAAAAACCCTGGGTTTTCCCCAACTCTTAACCGCCTTGGAAAGCAAATCCCCCCTTTTCCCCCCAGTGGGGGTTTATTACCAGAATAAAGCCGCCCCCCAATTCCCCTCCCCAAAATTTTTCCCCCCCTAATTGCAAATTGGAACCCCCCTGTTACGGCCCTTTAACCCCCGCGGGTGTTTGTGGTTCCCCCCCGTTAACCTAACTTTCCAGCCCCCAACCCCCCCCTTTCCTTTCTCCCTCCCTCCCCCCCTTTCGCGGGTTCCCCTCAACCCTAAATGGGGGCCCTTTG RQ ''$,.--+'+)*'')'.)/*+01*,-+99>AA>>>>===?B?7<1*+?9?AD==A=7.5954=>B:@==AE?A?A?EBA??B>?@DAB:5597:?;?@?AAB??DFBBE?AAAAABBBA=====BEAEABBAAAABBBAAAABCBB8B?AA=:===>AAAA?==D==>C?838---*.05?BEABA@@<8!.&(*-99=805!1-6<==553-.*57ECEAACC@BC?;>:8>@<EBAAAAAA=<C===@AA?883*9.22754:9?A??89.-2.=;;==@===F@AA??==7=B@A?@>@?A?ABABBBBA?B?AB=>=B>??BAABBECC>7??;33-43,22.?=>/8-/0/3360A9@=@747:7:;88;=:==AAAAAACC===A?=@=;:=@??;>@>?A87=876-)/,-67>@AA7@<<=@;1,,-14>=?://)((,())211*-5*0*+,***)+++$+.,3,.6'/))-&.+-9020)).++/0),,5,)())),.*,-..(*((),()*,/438,2/./*1.*35+.21,,&+)()'*(,+**))))$'%$$$$$$$$$$&&&('))))*-36-*-,)&(*/1*,)*()/)*(+*--*483181-/,/-,1**5;+)****,*)*((&+(*%*5**+%(++(+-06:75;2-0)**),,*)**++***))*001-0..**.,()**.8;BA-.0)))+$))))(+*--*++****)))))),,4530.*56.//9-*%((**-+.,-/)+))-+.%*,&,+)++-)'))11)((+*.66/,.2-,8511-...'&)4:1-((/+)))((,,))(+*-,/68.-)%++'*)+*+,*----*.+*(,),+)*0+)--.)))(*,>9/**(+*+%**-/1+02**--(*002-+0/032/*--/5**-/)*,()1,-)+11))**+*&+.,*).),++.++*.,03,()))*)*,)74..*,,4))/ TN 230375b_7 QL 5 QR 444 RT HAF3 5 25 = MIRA . RT HAF4 47 58 = MIRA . RT HAF5 59 86 = MIRA . RT HAF3 87 87 = MIRA . RT HAF5 88 160 = MIRA . RT KMRF 141 161 = MIRA . RT HAF3 161 215 = MIRA . RT KMRF 215 235 = MIRA . RT HAF5 216 262 = MIRA . RT KMRF 243 263 = MIRA . RT HAF3 263 269 = MIRA . RT HAF5 270 296 = MIRA . RT HAF3 297 318 = MIRA . RT HAF4 319 330 = MIRA . RT KMRF 330 350 = MIRA . RT HAF5 331 367 = MIRA . RT HAF3 368 368 = MIRA . RT SRMr 368 368 = MIRA . RT HAF5 369 436 = MIRA . RT KMRF 382 411 = MIRA . RT HAF4 437 444 = MIRA . ER AT 1 440 5 444 RD 230375a.t7 RG 1 RS TTCGAAGGCAATATCGTGACGATATGTATGATAAATAC*TGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCCGCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAACGGTGATCACAGGATAGGTATTTTTGCCAAGAGTGCCATCCAGACTGGCGAAAAGCTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTCGGCATCGAAAGAGAAATGGAAATCCCTTGACATCTGCTACCTCCTCCCCCTCCTCTGAAACAGCTGCCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAAAAAAAGAACATGCAGTTTGAAATTCTGAATTTGCAAAGTACTGTAAGAATAATTTATAGTAATGAG*TTAAAAATCAACTTTTTATTGCC*TCTCACCA*CTGC*AAGTG*TTTGTCCAGTGAATTTTGCATAATGCATATGTACATTTCACTTGATAAAATTCTGACTTGAAAAAAAAAAAAAAATTTATACGCCACTACCTTATAGTATTACTGCCGCCTTCACTTAGGAACTGGTACATACCTCACACACCTCCCTGGTATACAAGCGCCATGCCTCACATGCCCTTATGGATGGACCCCTTCGCCTTACCGCGGGTTTGTGTACCCATTACGTACTTGCCACCCTAACCCCGCTCCTTCCTTTTTCCTCTTCCCCCCATTCCGGGTTTCCCTAACTCTAAAGTGGGGGCCCTTG RQ '%)&=995+)+(+)+))*(.).*+//0,.2===;9/3*,/67A@A8;4.:?DD=====A>9====@>>:C:=AABAAAB===68>::>>=?:-.44:????B==::8=@CAA<A?@A?AAAA>===A=AAAAAAAAA?AAAAAAAA@@@AC@BA======A@@===:=@=>A@;:C3/-*326?@@AAEA>81'.%&*,5;<884)937==A@;:,6,6?AABEEAGB@@;;:94=A;AFAAABGG??>===@DAAC@;1;6965:19::B<?A75/1/5A?AAAAAAFAA@===:=8@?EAAAAA?B=>@=AA??BA@E??E<=9<8?BBBEEAAAAAA=:3377268;;;;/8+.584;;4==@>A==:==??9:3376;====A@=?====??===>?==@==E@?A?7;C?<5*7249@?BAAAA?AA?<?73699;?AE@==?A@AAAAA@A;<,,*(+,0@6.21103***.+3666601-)+/8@;:==E@==>?<;9772,341::30,14:64))0-820,*,,()'(..-.,4:?9931/52*))*,)))*-*14,*(01(*./1,++/36522<89.*-.,-*&2**(**/1.,++)-.-+,.,,(%++1+%+--,,(.,(00/+&+0.+3,,*+(.+,,-03/.,++)((,0,+1..+622*,-)(+*+1+++-++('.0*),&()*/.(*+)*))0(),,*((-++)+('02*//.--)(%((*,2/1..-,8),/-*((,+*,*2.+*(+1+.20-.,,)+))*5..0*-+,/*' TN 230375a_7 QL 8 QR 487 AO 1 38 1 38 AO 40 437 39 436 AO 439 461 437 459 AO 463 470 460 467 AO 472 475 468 471 AO 477 481 472 476 AO 483 789 477 783 RT HAF3 8 28 = MIRA . RT HAF3 40 49 = MIRA . RT HAF4 50 61 = MIRA . RT HAF5 62 89 = MIRA . RT HAF3 90 90 = MIRA . RT HAF5 91 163 = MIRA . RT KMRF 144 164 = MIRA . RT HAF3 164 218 = MIRA . RT KMRF 218 238 = MIRA . RT HAF5 219 265 = MIRA . RT KMRF 246 266 = MIRA . RT HAF3 266 272 = MIRA . RT HAF5 273 299 = MIRA . RT HAF3 300 321 = MIRA . RT HAF4 322 333 = MIRA . RT KMRF 333 353 = MIRA . RT HAF5 334 370 = MIRA . RT HAF3 371 371 = MIRA . RT SRMr 371 371 = MIRA . RT HAF5 372 440 = MIRA . RT KMRF 385 414 = MIRA . RT HAF4 441 463 = MIRA . RT HAF3 464 487 = MIRA . ER AT 1 480 8 487 RD 230375c.t7 RG 1 RS ATGCGAGCAAGATGGGACGATATGTGGGATAAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCCGCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAACGGTGATCACAGGATATGTATTTTTGCCAAGAgAGCCATCCAgACTGGCGAAAAGCTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTCGGCATCGAAAGAGAAATGGAAATCCCTTGACATCTGCTACCTCCTCCCCCTCCTCTGAAACAGCTGCCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAAAAAAAgAACATGCAGTTTGAAATTCTGAATTTGCAAAGTACTGTAAGAaTAATTTATAGTAATGAgTTTAAAAATCAACTTTTTATTGCCTTCTCACCAGCTGCAAAgTgTTTTGTTACAGTGAA*TTTTGCAATAATGCAGTATGGTACCTTTTTCCACCTTTGAATAAAGAAAACTTGAACTTGAGGGACCCCCCGGGGCCGGGTCTACTTTCTTTTTTTTATGCCGGGCGCAAACCTTAACCCCCTTAATTGAGGGGTTTAATTTTAACCTTGGGCCCTGGGCCCTTCTTTTTTACAACCTTCCTTGACGTGGGAAAAACCCCTGGGCGTTTCCCCCACCTCTAATTCCCCCTGGGGGAACATATCCCCCCTTTCCGCCCAACTTTGGGTTTTTTTACCAAAAGAAAGGCCCCCCCCCGATATTCCCCCTCTCCCAACATTTTTGCCCCCCCCTTAATTGGGAAAATTGGGACCCCCCCCTTTTCCGGGCCCTTTATACCCCCGGGGGGTTTTTTGGTTTTCCCCCCCCCTTTACCCCCTTCCATTGTCCAAGGCCCCTTAGGCCCCCCCCCTTCCCCTTCTCTCCCCTTCCTTTCCCCCCCATATTGCCCGGTTCCCCTTATAACTTCTTAATTTGGGGGCCCCTTTGGG RQ '&$).35,1-%-)/)'*,(-/++3-,+*2.=9489;:;=>DAA17***9<@CC?@=;3+/174@:=;?::BAAAAAAD??;@7/1:9?A915256;::@;BDB??AAGAAABEACCAAAA>@A=:?5>AA@F@?D@AAAA@CDA><5*873?9====?A>?;9;5?===@C:0:1..)566DCCCC??8A8./!0,4?97212!3.6@==956*/.6CCAEEE=@??;88241:=?BCA??A<AA5:@>B@DAAA?;.<4334.,1469:5709,3,3:<====>=F@CB?D==55BBGA??????AAABB???C???CB>5848>>CCDAC>8.10*)12/7767958,1&&..48,*22=:A?A==;=@@?E!7::9?=<=AA>9=/.-+*+1/6377801.06<0,1*)(1*).!&.4?A84,*(/3.4*-!133.++*+)***1139544,/**+'*(&0-27(&,)'-*2*!+!*343((.)-*-+*3:51..('),1/,+*))(%)'+(**(+(,534(),))+596-/2,20<041)+-,2)-+)+()*&&(*.*,-&&&&$$&&&%%&&)*&'),*122*,-0*.&*(*.+()*(*-)+,,**)*643()0*,-***/0/4.+-++,39<4+,**)024,,,)+-,,0-()*(756,+)()+(')***-(()))+++--2365.1/,+++))'+-+(-33*-'),')),)+,,17-.+,+(()%$)()+)+/?D<,0.(++50++%),()22+&-3.*-*))+)-00,0-,,+*+-,2-571(*.)**08-+)))))()**)+,+3,,**64A8984-/1++-+,++1+*-(+0)**.5664-+3-0+*-,,-*..,/*,)+*/77-2,1))))+*2.*))**+(*..24-*+*,,--****(*''''),)('(*(&()+)))&.(),356;886-4.,.)))'(*,)+,***(112*))),*),(%.&,*)))(,/09./,*(()+..)&)*)(***)%,**-****(,,*)** TN 230375c_7 QL 28 QR 519 AO 1 401 1 401 AO 402 476 403 477 AO 478 494 478 494 AO 496 1023 495 1022 RT HAF3 28 47 = MIRA . RT HAF4 48 59 = MIRA . RT HAF5 60 87 = MIRA . RT HAF3 88 88 = MIRA . RT HAF5 89 161 = MIRA . RT KMRF 142 162 = MIRA . RT HAF3 162 216 = MIRA . RT KMRF 216 236 = MIRA . RT HAF5 217 263 = MIRA . RT KMRF 244 264 = MIRA . RT HAF3 264 270 = MIRA . RT HAF5 271 297 = MIRA . RT HAF3 298 319 = MIRA . RT HAF4 320 331 = MIRA . RT KMRF 331 351 = MIRA . RT HAF5 332 368 = MIRA . RT HAF3 369 369 = MIRA . RT SRMr 369 369 = MIRA . RT HAF5 370 437 = MIRA . RT KMRF 383 412 = MIRA . RT R454 398 399 = MIRA . RT HAF4 438 443 = MIRA . RT HAF5 444 485 = MIRA . RT HAF3 486 494 = MIRA . RT HAF5 496 518 = MIRA . RT KMRF 499 519 = MIRA . RT HAF3 519 519 = MIRA . ER AT 23 514 28 519 RD 122533a.t7 RG 1 RS GTTCGAGCAATATCCCTACGATCAACTAGAACAATGATTTTGTGGTGGATGCAACCCGCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAACGGTGATCACAGGATAGGTATTTTTGCCAAGAGAGCCATCCAGACTGGCGAAGAGCTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTCGGCATCGAAAGAGAAATGGAAATCCCTTGACATCTGCTACCTCCTCCCCCTCCTCTGAAACAGCTGCCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAAAAAAAGAACATGCAGTTTGAAATTCTGAATTTGCAAAGTACTGTAAGAATAATTTATAGTAATGAG*TTAAAAATCAAC*TTTTATTGCCTTCtCACCA*CTGC*AAGTG*TTTG*TACCGTGAA*TTTTGCAATAATGCCATATGGTACATTTTCCACTTTGAATAAAGAATACTTGAGCTTGAAAAAAAAAAAAAAAAAAGATTCTTTATTTAAGCGGCCGCGAGCTTTTCCCTTTAATAGGGTTAATTTAACTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX RQ &&&,1/?/,/(+)+'('+)(1.+*+,0**1-1574=====EBAABA=:=56;6879=>7-025<???@BA>>>;?ACBA?A?AAAEBEA?ABAA=FF@@??AA?@AA?A=@=A<<ACAAAD?==>@==@=@E=@@A@=A@;2:22-&623@DFBAABB=59;/888B?B?<72;;:@==84.)1,9AAEEFECB???;B?<>??;BCBAAAAAA?==>=@DA??;7.<8<524/37AA??><.,1.87====AEB@AAA===869BA@?=@=?AAAABBABBABAAAAAC==A??ABBBEEDAEEBA97:053064:A7;)2,,2.63:5==>=@:::A@;A=7A==A?AAA?A=9====??=D====B@@=:E=A@AAA?;4:6=64==?AABAB05**33/,.116>E@==9=:=;:?@=:A=466,-)!*..--./12310627:636668;.,*(1,9976E<6;5@=8=@8-(/))..033AAA:992/2/>674;65936400,-.62++,$**-8;34.**)118''4.**)+)))).11,**..-(.()+*))+$)$+')12,02*,.-,+*(,6*)*)1)(..8'!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! TN 122533a_7 QL 27 QR 517 AO 1 404 1 404 AO 406 417 405 416 AO 419 431 417 429 AO 433 437 430 434 AO 439 442 435 438 AO 444 448 439 443 AO 450 453 444 447 AO 455 463 448 456 AO 465 968 457 960 RT HAF3 27 28 = MIRA . RT HAF5 29 56 = MIRA . RT HAF3 57 57 = MIRA . RT HAF5 58 130 = MIRA . RT KMRF 111 131 = MIRA . RT HAF3 131 185 = MIRA . RT KMRF 185 205 = MIRA . RT HAF5 186 232 = MIRA . RT KMRF 213 233 = MIRA . RT HAF3 233 239 = MIRA . RT HAF5 240 266 = MIRA . RT HAF3 267 288 = MIRA . RT HAF4 289 300 = MIRA . RT KMRF 300 320 = MIRA . RT HAF5 301 337 = MIRA . RT HAF3 338 338 = MIRA . RT SRMr 338 338 = MIRA . RT HAF5 339 407 = MIRA . RT KMRF 352 381 = MIRA . RT HAF3 408 422 = MIRA . RT HAF3 430 455 = MIRA . RT HAF5 496 516 = MIRA . RT HAF4 517 517 = MIRA . ER AT 53 543 27 517 RD 122533c.t7 RG 1 RS ACTGAGCAAATCGTTACGATCAATAGAACAATGATTTTGTGGTGGATGCAACCCGCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAACGGTGATCACAGGATATGTATTTTTGCCAAGAGAGCCATCCAGACTGGCGAAGAGCTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTCGGCATCGAAAGAGAAATGGAAATCCCTTGACATCTGCTACCTCCTCCCCCTCCTCTGAAACAGCTGCCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAAAAAAAGAACATGCAGTTTGAAATTCTGAATTTGCAAAGTACTGTAAGAATAATTTATAGTAATGAGTTTAAAAATCAACTTTTTATTGCCTTCTCACCAGCTGCAAAGTGTTTTG*TACAGTGAA*TTTTGCAATAATGCA*TATGGTACATTTT*CACTTTGAATAAAAATACTTGACTTGAAAAAAAAAAAAAAAAATCTTAATAACGGCGCAGCTATCCTTATGAGGTATTTACTGGCCGGCGTCTTACACTCTGATGGAACCTGCTACCACTACGCTGCACACCCCTTCCACTGCTATACAGAGCCACATCCTCCCATGCCACGATGCATGACCCGTCGCCTACCGCGTTGTGTCCCCTACTAATCACCCACCGCCTCTCTCTCTCCCTCCGTCCTACCATGGGCTAGTCATTCTCGCCACAAATATGTA RQ ''&$),-0.,&'&)('))--(('(('..1874E;8;=AAAAAA;9A99<1849;>6*/24<;:6=>;9474;?BBA@AE@AAAEAA?ABBA=@@@@?AAEEAA@@>=@===<=AAABA?>====?AAAA@@@A=AA@=:<5-++..-?@ABBAA?:2,7-736B;?892.9:7?==66/)1+3?@EEEEA@D@@;B>8=@D?AF@?AA?A=AA?A?AA??<:6,;6=6661994<=:99.,3,97:=:====?@B??=C>5C@?EE??A?A?AAABBABAAAAADAD??C@AAEFBAEAAAAAA==;520/05.@9@23.+658;;5=8?<;:=:@@9<4*55::=AAADA?;??>CCA?B====BA?A?AAAAAAAAB9:7<88>==AA@<AABA25.1,76=??CB?D<ABABAEAAAAAA@@@>?=;@=8=4;33431..89@=E>100(*1.A7>135,*46AA>8=E;22,,,,,,/-4250:E433273E-30/200*23+1/,+),+*/1=77+*659<2758.,,)*.*)(&'(',+2+.,*.*(.(/.2-8/*-3-3-1=4;:0+.***033-/+,/*0)2.+,)&),,6,/,0%-//33..)))+86.-),(*5133//.+02%-1/9+01+-,*,))*)+,.))).)),-*(26;+3--1,.((,+-/--7//7,0,*8.70*6-%.*)))(+,1)102),)(),,-,28@9<*),1**<=96,***(/36,-+(/9+,,*(+)*0.9;56++-,+.) TN 122533c_7 QL 24 QR 504 AO 1 450 1 450 AO 452 460 451 459 AO 462 476 460 474 AO 478 490 475 487 AO 492 769 488 765 RT HAF3 24 25 = MIRA . RT HAF5 26 53 = MIRA . RT HAF3 54 54 = MIRA . RT HAF5 55 127 = MIRA . RT KMRF 108 128 = MIRA . RT HAF3 128 182 = MIRA . RT KMRF 182 202 = MIRA . RT HAF5 183 229 = MIRA . RT KMRF 210 230 = MIRA . RT HAF3 230 236 = MIRA . RT HAF5 237 263 = MIRA . RT HAF3 264 285 = MIRA . RT HAF4 286 297 = MIRA . RT KMRF 297 317 = MIRA . RT HAF5 298 334 = MIRA . RT HAF3 335 335 = MIRA . RT SRMr 335 335 = MIRA . RT HAF5 336 403 = MIRA . RT KMRF 349 378 = MIRA . RT HAF4 404 409 = MIRA . RT HAF5 410 454 = MIRA . RT HAF3 455 476 = MIRA . ER AT 53 533 24 504 RD 122533b.t7 RG 1 RS CCGAGCAATATCCCCACGATCACTAGAAAATGATTTTGTGGTGGATGCAACCCGCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAACGGTGATCACAGGATATGTATTTTTGCCAAGAGAGCCATCCAGACTGGCGAAGAGCTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTCGGCATCGAAAGAGAAATGGAAATCCCTTGACATCTGCTACCTCCTCCCCCTCCTCTGAAACAGCTGCCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAAAAAAAGAACATGCAGTTTGAAATTCTGAATTTGCAAAGTACTGTAAGAATAATTTATAGTAATGAGTTTAAAAATCAACTTTTTATTGCCTTCTCACCAGCTGCAAAGTGTTTTGTACCAGTGAATTTTTGCAATAATGCAGTATGGTACATTTTCAACTTTGAGATAAAGAATACTTGAGCTTGAAAACAAAAATGTATCTTTATTAAAGCGGCGCAACCTATATCCCTTTTAAGTGAGGGGTTAATTTTAACTTTGGGCCCTGGCCCTCCTTTTTTACCAACCTTCTTTGAACTGGGGAAAAACACCTGGGCTTTTACCCAACCTTTATATCCGCCTTTGGGCAAACAAAATTCCCCCTTTTTTCCGCCAAAGCGGGGGGTTTTTTTTTTACCCAAAAAAAAAGGGCCCGCCCACCCCCTATATTTGCCGCCCCTCTCCTCCCCAAAACAATTTTTCCGCCCCCCCCCCCTTTAAAATTTTTGGGCGCCAAAAAATGTGGGGGGGAAACCCCCCCCCCCCCCTTTTTTTTCCCCCGGCGCGCCCCATATTTTTAAAAACCCCCCCCCCCCGGGGGGTGGTTTTTTTTGTTGGGGGTTTTCCCCCCCCCCCCCCCCCCCACCCTTTTTTTTAAAAACCCCCCTCTATTTTAAAACATCTTTTTGTTGGCCCAAAGACCCCCCCCCCCCCTATTAATAAACCCCCCCCCCCCCCCCCCCCCTTTTTTTGCCCCCTTTTTTCTCCTCTCTCCCCCCCCCCCTTCCCCCCCCTTTTTTCCCCCCCCCCCCCCCCCCATTTTTTTTTTTTTTTTTCCCCGCCCGGGGTGTTTTTCTCCCCCCCCCCCCCTTTTTTAAAAAAAAAACCCTCTCTCTAAAAAAAAAAATTGCGGGGGGGGGGGGGGGCCCCCCCCCCCTTTAGGGGGGGGTTTCTCCCAAAAAATTTTTATTTTTTTCTTTTTTAGGGGGGGCCCTCCCCCCCCCCCCCCCCCCCAAAAAAAAAAATTTTTTTGTTTTTTGGGGGGGG RQ '$(-,-4-)'()**'/4+7*+**,()8)(4/16458AAEAD?368/1:14489>5*-13;@::8D>>>>8>BABB@AAAF?AAA?=@BBA=??A@AAAAAAAABA=@=:=<AAAABA?==>D>?AAAA@=@@=BF@=8<6+,(../@ACBBEAA>44718:ADA@;;99:7:DBC650)2+7AAAFFAA????<;?<??C@@@@??A?A=<==8=AB@?C:6,>==;<<5<=AA?A<<..3,78@==B===@AAAAA@???@AA??A?ABAEEAABBBA?A?A=@@<;9@?ABFBFEBBAAAB>;9453054;B?A05..87=A?=AABAA9:A?A;E6<<9>==AAABAAAA???CAA?=====??=>@=<<8;?=A9:3;;7EAABABAEAAA566:69A==@@EAA=@==@EDCAAAA>====?====:=AAAA==B@===>=@@9:=541.52371=E@4;;9;4@>@>=@?=55666)*,(),--/@;?@120,*)-**./:11(*(++')'.+)'&&&&&'''+)*)+')01-04**(,-2,,**-,.*./52./62722--++,)-(*.2+()+---3//1**2986.0-3-+.-*''()-',.((%(,%*,))))))))*42/05<-24*%+(..*+0+**.62,011+))*+,+-,)*+.-,,())),+)('))*,**))1201.)/52.**+'.-*)))')).40***562/,,,)000,07:DBC0/,,+,))+&-),())).)*+++)+'(&.&.-0>66,*-)1++'-+)*(*+14.,))*(*.),-1772***%*.2,,*&&&%,(()()(,+-**)()*,,7463)*&)*+-.:17DD3+)))))))))*(**'()&&))&&*)(%)()1/*./-'*%(,+2.)&...+)))'+*'&&)))()*1,-)(+(+**,.+))&&'+)*))2.-.2*.**-('))&))**2+,*)&+((()))-))%))&))))))))))$$--0)))')'&)***&)+((((-114*2-+*))$)),(),(&**086><6.-*,)+65=D76),*16/)))+*'*(+*59/.())**.++$--1DE5/-,-)+)(*,,0+,.3)+*+)&&**+**-,-/*./+((()%&++988A8A774.*(&'(+()))-)*0,%(***-.*2-02<BB>94*())))')(')41+/****&))'+'++,*)&(+,,=/-)(()+-()),2A@ED5<A0;2*(.*)53@;23,*-*%)))3285,+**,),**)(*(((,***.&*),,-**%1289,(&-*.*)**,,.%*..3-)+*,+)))*)).0*&*,/5..*)*'*(-,))))))*)0))**,)-.6 TN 122533b_7 QL 29 QR 489 RT HAF5 29 52 = MIRA . RT HAF3 53 53 = MIRA . RT HAF5 54 126 = MIRA . RT KMRF 107 127 = MIRA . RT HAF3 127 181 = MIRA . RT KMRF 181 201 = MIRA . RT HAF5 182 228 = MIRA . RT KMRF 209 229 = MIRA . RT HAF3 229 235 = MIRA . RT HAF5 236 262 = MIRA . RT HAF3 263 284 = MIRA . RT HAF4 285 296 = MIRA . RT KMRF 296 316 = MIRA . RT HAF5 297 333 = MIRA . RT HAF3 334 334 = MIRA . RT SRMr 334 334 = MIRA . RT HAF5 335 402 = MIRA . RT KMRF 348 377 = MIRA . RT HAF4 403 408 = MIRA . RT HAF5 409 483 = MIRA . RT KMRF 464 484 = MIRA . RT HAF3 484 489 = MIRA . ER AT 59 519 29 489 // EC CO MIRA_rep_c3 NR 6 LC 454 CT MIRA 1 5 = . Note=Assembled with MIRA CT IUPc 23 23 = . Note=Strain 'StrainX': W CT H454 240 240 = . Note=No edit. PL: 2 RS: 5 RL: 0 RWTS: 0 EL: 0 CT IUPc 279 279 = . Note=Strain 'StrainX': R CT IUPc 286 286 = . Note=Strain 'StrainX': Y CT IUPc 292 292 = . Note=Strain 'StrainX': Y CT IUPc 295 295 = . Note=Strain 'StrainX': W CT IUPc 325 325 = . Note=Strain 'StrainX': M CT IUPc 352 352 = . Note=Strain 'StrainX': M CT IUPc 370 370 = . Note=Strain 'StrainX': K CT IUPc 381 381 = . Note=Strain 'StrainX': V CT IUPc 397 397 = . Note=Strain 'StrainX': Y CS gcgttattaaagacttttttttwtttttttttcaagttcaagtattctttattcaaagttgaaaaatgtaccatactgcattattgcaaaaattcactggtacaaaacactttgcagctggtgagaaggcaataaaaagttgatttttaaactcattactataaattattcttacagtactttgcaaattcagaatttcaaactgcatgttctttttctaaattgcccacagtactcgaggttcctgaagctaaggcagctgtttcagaggagggggargaagtaycaaatytcwagggatttccatttctctttccatgccgamatacttcagggcatcagcctgactgtmtctgtaatcaaaaaacakctcttcgccavtctggatggctctctyggcaaaaatacctatcctgtgatcaccgttaaccatcataacttttgcatagcattt CQ &(*(70+=9;3)+(*.?DDH::**BbhPORHOPYY\]\]ZUJIJNY_b]WTST]YO[b_Z^b]a__]`b[[^\c`eggfddeZ^UZ\]^`Y\\UVVMWaWf`dbfaaaaac^^ge_^LOW[aadYZ]W]_cekdffgfl_\cagcdaegfebbdhcieecfefgebb]WVY]Pee`db`b`^\ge__de_b`Z`[_\]j]gddddgb]UQSCU^W\^N]^gijfffeec_]RPPHXUCK(?GFSXXVRSG[WLSXTMS\NT]Wc^d[(\JRRFWVTYB-.7,/0-*3-;27*58.0/42:<:@GBBDB=;32-64=);*9>><;/;AA@><864=<AE>>/0;=2>*4:3=,7<:3D7+<=7817G@6)3@A@@8<-<;3*79/<4213739;7:9/452<;??A:99734.68/06/1/178A?H4?@><5:>7<65:7?@AAC441%**,01 \\ RD 122533c.t3 RG 1 RS CTTGCGTTATTAAAGACTTTTTTTTTGGTTTTTTTCAAGTTCAAGTATTCTTTATTCAAAGTTGAAAAaTGTACCATACTGCATTATTGCAAAAATTCACTGGTACAAAAcACTTTGCAGCTGGTGAGAAGGCAATAAAAAGTTGATTTTTAAACTCATTACTATAAATTATTCTTACAGTACTTTGCAAATTCAGAATTTCAAACTGCATGTTCTTTTTCTAAATTGCCCACAGTACTCGAGGTTCCTGAACCTAAGGGCAGCTGTTTCTGAGGATGGGGAGAAAGTACAAATCTCTGAGGATTTCCATTTCTCCTCCCACCTCTAAATACTCCGCGGCATCTTCCTGAATGTCATATGTTGTGGGCAACTTCTCTTCACAACTCTGGATGGGTCTCTCTGAAAAAATACCTTTCCGTTGAAACTCCGTTAACCTTCTTATTTTTTGCATCCCCGTTTGGATGCCCCCACCTCATCGCCAAACTATTTTTTTTCCCCCTAACTGGTTGCAACCACCCAAAAACTTTGTTCCACTACCCGAACCAATAACTTCGCCCCTATGGCCAATCCCTAAAATGATTCTTTTTTTTCCGGCCCCGTCCTACCCCCCCCTGTTGTGGATTTGGTTTTCCGCTCGCAATTCCCCCTCGTTTTAAACCTGAAACACTGAGGTATAACCCCGGGGGTTCCCAAATTAATTACCTCTCCCACATTAACCCCTTTCCCCCCCACTGCCCGTTTTCCAGTAAAAAAACCCTTTCCTATCCCCCCTCCCCAATAAATTCCCCCCCCCCCCGGGAAAAAGACGGATACCTTCTTTTGGCCCCTCCCCCTCTTCCCCCCCCGTGTGTTATTGTTTGTTCTCCCGCCTTTCTGGCCCGGAAAACTGTGTTTAAACTCCCTCTAGAGAGGCGGTCATTAGGGTTTCCCCACATTCCGGGGTAATCCCCGGTAAAAAAACTTTTCCCCGGCGCGCCCCTAAGGGGGGAGAGAGCCCCAAGGGCGTTTCTCACAGTGTTTTCTCTTAGGCCCCCCCCCCAAAAAAATAATTTGGGTTT RQ &&$)2*(1/*532-))&(.7AAE72&*,&49AA>;=@;AAABA636166?A34566;:57<?::@74(!+53857=DAC=@@AAED@>B??;;<???@A@@D@@C@A81)!*,64=?A;/>>>??9=.0.*.17:=@=@?;;818;??@A?CDDAACF@>=9??ACA<C?C@<C?:>@@@@;?73/88.07C9;/2618?ADAC@@AADA>7>+8;7A<7:4?C@@B@CDDA98*.34A643057698-+24,/*-.),*)((()'+933((1*0,),1191)()212//---*(/)%(),*+.0.222.-211****----*%,&..//-**%*)%4:05993-;7<23)(*11,)))++))*(,/*,.,10.2.()1*(*/)***,.42*-)(3*-<,*,-,*52,*4.-/,(-**)*0))'+$)/,.;:0-4+,-*,,*0421/-,+5,(*)%..+0*+/(),5/33*)(+*(()),)**(+,,5:9*(**)**-,*))*(.*,.**)))**,,**9>7+**)**+.*)'')%(),)++.-*+()(,**)*,+,3.-3//464=4-79)/5;.*)***(++.((/,,3..559/+)(*,*++*,))(-**)**+,)))(-))*/-*,,*,)*+),--2+***,*+)&2731-7/,-*)*-*-/*))**(**-,-9-01*,-+**)/53*-,-***.-))*(+**+,)++++)()*01*0+*-1.-+*))*+*+,)*.0**,(%**)))+--)*.&*++,(()())27+*++*(*,*+++**+,;4/*-53,0()((*+6++*(())(+)(,')-)))(*)(/,,)))++*+*0+*1156-*(((.-****+1)**+**))'+))**+*-,)()-47776;30.),00,+))0*()(*)+++,+(*+*))++*)%*(&%1**,/1,**).**),*)),**+))**13,+)))),***)%*.1:8;;)*..5551-/-,.,-,+)*-33-*)))),**-),10*())*))))*0+*)-001/*,)*+,+20>;::950526/)**-))*+*()*,,, TN 122533c_3 QL 4 QR 259 RT HAF3 4 40 = MIRA . RT KMRF 39 91 = MIRA . RT HAF4 41 41 = MIRA . RT HAF5 42 70 = MIRA . RT HAF3 71 71 = MIRA . RT HAF5 72 146 = MIRA . RT HAF4 147 152 = MIRA . RT HAF5 153 252 = MIRA . RT KMRF 178 207 = MIRA . RT KMRF 212 232 = MIRA . RT CRMr 221 221 = MIRA . RT HAF3 253 259 = MIRA . ER AT 1 256 4 259 RD 122533a.t3 RG 1 RS ACGCGTTATTAAAGACTTTTTTTTTGGTTTTTTTCAAGTTCAAGTATTCTTTATTCAAAGTTGAAAaATGTACCATACTGCATTATTGCAAAAATTCACTGGTACAAAACACTTTGCAGCTGGTGAGAAGGCAATAAAAAGTTGATTTTTAAACTCATTACTATAAATTATTCTTACAGTACTTTGCAAATTCAGAATTTCAAACTGCATGTTCTTTTTCTAAATTGCCCACAGTACTCGAGGTTCCTGAAGCTAAGGCAGCTGTTTCATAGGAGGGGGAAGAAGTATCAAATCTCTTGGGATTTCCATTTCTCTTTCCATGCCGACATACTTCAGGGCATCTTCCTGACTGTCTCTTTTTTCGGGAAACTTCTCTTCGCCAATCTGGATGGCTCTCTCGGCAAAAATACCTATCCTGTGATCACCGTTAACCATCATAACTTTTGCATACCACTTTGGATTTACCACCGATATGCCACAACGATTTTTGTTACCCTTGCAGGTTGCACCACCCAAAATCGTTGTTCTTTTGCCCTTGCCAAATTCTGGGCCCCAAGGGCCAATTCCCCAAAATTAATCCTTTTAATTTCGTGGGCCGCGTTCCAGCTGTCCCCCTTGTTGAAGGTTTTTTTTCGCCCCCCGGCTCTCCCCCCCCCTTTTTAAACCCCGTAAACCATTATAGTTGGTTAAACCCCCGGGGGGTTCCCCCTTGTAAATTTTACCCCCCCCTCTCCCCCATTTAAAAATATGTGCCCTTTTTCTCCCCCCCACTGGGCCCGGGGTTTTCTCCTATTCCGGGGAAAGAAAAACCCTGTTTTCTCTTCGCCAAAATACTCCCCCCCCCCTTTTTATAAAAAAAAAAAAAACTTGGGGGCCCCCACCCCCCCCCGGGGGGGAAAAAAAAAAGCGCGGGGTTTTTGCGCGTTTTTTTTGGGGGGGGGGGGGTTTCTTTTCCCCCCCTTTTTTCCTTCCCCCCCAAAGGAGAAAAAATCTTTTTTGTTGGGGCGCCTCCCGGCGCCCCTCCCGGGGTTGGCGCGGCGAAAACGCGGTTTTTCCCCCCCCCCCAAAGGGGGGGGCGCAGATACCGCGTTTACTCCCCATAAATACTCCGGGGGGGAATAACATCCCGGGAAAAAAAAATTTTATATCTATATATGGGGCCTCCCAAAAAGGGGCATAAAACACGGAGAAACCGGCGCCCTTGTGTGGGCGTTTTTCTCTCAATGGGCGCCCCCCCCCTCAACAAAAATAAAAA RQ '%+2*(6.+;8:2)+(*).1AA88(-,(88AA=;==99@;BC21-,,-8?91695?=5:7?;11,)!*-55==@:BABBAEABABA?BA??;<?<7AAAA?A<<B=9:620576:;@;4>>;@;;7*0/*22;@BCCBE@F?<??DACAAABDAACD?=<B?BB@DA@?B@<@=9B>@??8??678>0,6?9@>8>;?CAEAD??>@DEA>?4>A?C?8;7:A?ACCAADB>=-5;3>:/6-3:6@A4597->58:6318=B?DAA?72*/+.1)01771-.1.+/+).(610)-7-(,0-8968?:/A/4928,))-+1))21/1-6??>9:3-572629.)2510-+,,/5,3,*+.)*.)*+/.7>-..00,+.40:82+68.;2/,-.)+.-+*)**)626;?988623-37./5/1))78@>G<??>;5:>7:/--68;8?A40/,.1--.5:768.4))(())(+%)))+*))**((+(*(*0*--/;::2,+()))++)**,--:2**2/2(*)+,*))+((0-,/*))*+)*+-2+()++/**,,*-=751,,)((*25;(,,2),(-.(*,-+1-.,,./-%.%*1,,'()+)+('%.*)*))0,&(+(),),+++,/*./*,*,))))*)(%%%+-%**,3.+)0**202%-0,,20*/(*,*++))(0())()'(+))17:84.)+1.)))+))41,)$'''))'*0))&),3-,))++-)*+..*',,),+.+.((.**+++),*+,)%(.,/51+()''),.,*/--+(002/((,&+&*,*,+,,,*)*),*)-))*+*%.***,(+))$'&+%)*-'%*0.*+***///+.,,*)*),-*267<;30-(%)**(,1456;63)*,*,+=759.3-.342-*-+0())'))**,)200,)025))++++(*/466,',$))12*&///,)()*)/)+-),2-,**,*1.1.,***,)/,22-*%)),*&/)+',,++)/,**)((()))*,%(*)+)))(*&)).03&1;5:22))+))'.-))+&+/+)))((+*+,33=36-,,3//,+)+)),./+,)*+))*(*)))))%)),)$'+)+,*&)+++,-))**++7702&**1+)-*(*-+)+*)))/1.0.+,-)*)(**.*)**')'')$*.-)42)**00),+*)))%*,)).+.-.****(()(+)-)(()*.())((-*,(++//+,1*.-()/31/8/,/47;7,+(),324)*))(**0C@ TN 122533a_3 QL 3 QR 450 RT HAF3 3 39 = MIRA . RT KMRF 38 90 = MIRA . RT HAF4 40 40 = MIRA . RT HAF5 41 69 = MIRA . RT HAF3 70 70 = MIRA . RT HAF5 71 145 = MIRA . RT HAF4 146 151 = MIRA . RT HAF5 152 257 = MIRA . RT KMRF 177 206 = MIRA . RT KMRF 211 231 = MIRA . RT SRMr 220 220 = MIRA . RT KMRF 238 258 = MIRA . RT HAF4 258 269 = MIRA . RT HAF3 299 359 = MIRA . RT HAF3 400 427 = MIRA . RT KMRF 427 447 = MIRA . RT HAF5 428 450 = MIRA . ER AT 1 448 3 450 RD 122533b.t3 RG 1 RS ACGCGTTATTAAAGATTTTTTTTTTGGTTTTTTTCAAGTTCAAGTATTCTTTATTCAAAGTTGAAAAATGTACCATACTGCATTATTGCAAAAATTCACTGGTACAAAACACTTTGCAGCTGGTGAGAAGGCAATAAAAAGTTGATTTTTAAACTCATTACTATAAATTATTCTTACAGTACTTTGCaaATTCAGAATTTCAAACTGCATGTTCTTTTTCTAAATTGCCCACAGTACTCGAGGTTCCTGAACCTAAGGCAGCTGTTTCAGAGGAGGGGGAGAAAGTATCAAATCTCTAGGGATTTCCATTTCTCTTTCCACGCCGAAATACTTCACGGCATCTTCCTGACTGTCTCTTTATTCGGGCAACATCTCTTCACCACTCTGGATGGCTCTCTCGGCATAAATACCTTTCCGGTGATCTCCGTTAAACTTCTTAATTTTTTGGCATCCCCGTTTGGGATGTACCGCACTGAATTGCAAAAACTAATTTTTGTTTACCCCTTGACGGGTTGCCATCCCACCCCAAAAATCCTTTGTTTCAAATTTGCCTCCGTGACCAAAATAACCTTGGGCCCCCCTAATGGGCCAAATTTCCCCTCATTAGTTTGAATTTCCTTTTTAAATTTTCCCTTGGTCCGCGTTTCCTAAACTCCCTCCCCCCGGTGTTGAGGGTTTGGTTTTTCCCGCCTCGGCAAATTCCCCCCCCTCACTTTTTAAAAACCCCTGTAAACCCACTTGGGTTTTTAAACCCCCTGGGGGGTGTCCCAAAATTTAAAGTTTAACCCAATCCCCACACATTAACTTCCCTTTTCCCCCCCCCTGGCCCGTTTTCTCCTTTCGGGAGAAACCCGTTCTGTTCCCCCCCTCCCTTAAAAAAAATTCCCCCCACCCCCCAGGGAAAAAGCGGTTATATCCTCCTTGGGGCCTCTCCCCCTCTCCCCCCCCCGAGTATATCTTTGGTGTCCCCGGCGCCTTTTTGCCCGGAAAATGTGTTATGCCCCTATAAAGGGGGGTCTATGGGGTTTCCCCCACACCCGGGTTCCCCCGTAATAAACCTTTTTCTCCCCGGCCCCCAGGGCGGAAGCCCCAAGGGGGGTTTCTCATGTTTTTTCATGGGCCCCCCCCCAAAAAATAAAAATTAG RQ &%,0*)2*)209.+)'.+=A?@68),,(<<AA??@=<;?>CD7456<:?A3.31/8=05.51880+*+-31====AAAAAA???B@>@A;9:9;@;@@????::?D9:520/52:A??;>><?==A9940.58>AA@BECC@>@C@BAACDAA@@@@:>97??A@BBC?=13*(*-3,7764.,.6<!!?;?22)2/4?ACAA?@@@@@:;9-;;<C?2=<?DA@A@=BAA<7*033;5,7,.36CC;-5/*1.0.*./:=??:?D26,(2+0.).02..%'64.+(,))91/(-,(1(-10-.+11..-;924++,<1:*)-2*0-9.42./0*';:7><<*'981=53./2))*3,,.)(()*,+.2.0218.)05%3'))(**/31/**(+/)28.,.)6&,,++)*+(/+0*)*2-0)(&,0,,/25*,*30/*,*.5C;;,),*)+**,(%*)0,*.))''*+4)/*),,.-+)*+*+5:=8*.+,3.1.)*.*),,-1)(+**(.**-**(*(,*)**+-1,%%(****)((+(+..*+*(..,--*+(/%)*++*88231.,,*/+,,*+**,,))))..))509-,21:@50))*$(*&-,(,,,(**)(*+.**'***005711%%*(%+*$+))/+4.2)((+*)))))),35()())))*0()+*-)-)*,+,.*.1,++)()*&)7/.+*..9;)(('))**/4/1%+369160)*+*+.2&,**(*&**)+**,*,*2**021&*263+*+*()+***.-'))))))*+++,*;6+)**)+))+'()*/,-)*)*,-6.,*(-,;A77+++,,***+11030,,*((())((..+***+))*)*)***6:79-+)))),*)*-.510,.)*,+/0,,,,31,,*)+*,8:60+0(****1*(&)*.((+(*+*)+-+,).++/+,&*,3:99..,+++**)*-+)(&)*,*1*+*))))'-+)/+**&)()%,-,133**)(*(+%%.)**,)%+*(***0++()()+()+).+))*+)**)))))57*-))/51(*)**,*)))(*/2,++*-1('**/+.-***,*))),))**),%,),5*+,*-/+)()))*7:5+(*(*+*+278DA851)))()*,.,++**)))) TN 122533b_3 QL 17 QR 403 RT HAF3 17 39 = MIRA . RT KMRF 38 90 = MIRA . RT HAF4 40 40 = MIRA . RT HAF5 41 69 = MIRA . RT HAF3 70 70 = MIRA . RT HAF5 71 145 = MIRA . RT HAF4 146 151 = MIRA . RT HAF5 152 251 = MIRA . RT KMRF 177 206 = MIRA . RT KMRF 211 231 = MIRA . RT SRMr 220 220 = MIRA . RT HAF3 252 281 = MIRA . RT HAF5 298 318 = MIRA . RT HAF3 319 320 = MIRA . RT HAF3 337 359 = MIRA . ER AT 15 401 17 403 RD 230375a.t3 RG 1 RS GTCTCTTTTTTAGAAATTTTTTTTTCAAGTTCCAGTATTCTTTATTCAAAGTTGAAAAATGTACCATACTGCATTATTGCAAAAATTCACTGGTACAAAACACTTTGCAGCTGGTGAGAAGGCAATAAAAAGTTGATTTTTAAACTCATTACTATAAATTATTCTTACAGTACTTTGCAAATTCAGAATTTCAAACTGCATGTTCTTTTTCTAAATTGCCCACAGTACTCGAGGTTCCTGAAGCTAAGGCAgCTGTTTCAAAGGAGGGGGAGGAGGTACCAAATTTCAAGGGATTTCCATTTCTTTTTCGATGCCGACATACTTCAGGGCATCAGCCTGGCTGTATCTGTAATCAAAAAACAGCTCTTCGCCAGTCTGGATGGCTCTCTTGGCAAAAATACCTATCCTGTGATCACCGTCAACCATCATAACTTTTGCATAGCATTTGGATTTTCCGAATTATTTGCTAAACGAATTTTTTACCCCTGGCGGTTTGCATCCACCACCAAAATCATTGTTTCATTTTGAACAGAAAACCGCACATTTTTTTTTTCATTACAACCCCCTTCCCAAATTCTGGGCCCCAAAGGGCAAAATCCCCAAAAATTGAATTCCTTTTTAAATTTCCCTAACCCCTTTTCTAAACTTGTTTCCCTTTTTTTAAAAATTGTTTATCCCCCCCCTCATTTCCTCCCCCACCCTTTTGAAAACCCGGAAAACCTTAAATTTTTTTAAACCCCTGGGGGTTCCCCCAAAAATTGTTAACCCCACCCCTCCCCTTAAAATTGCGCTTGTGTGCGCCCCCCACATGCCCCCCCTTTTCTTAATCGGGAAAGAACCTTTTTTTTCACCACTCTTCCTTTTATAATAAAAAAAATCCGCCCCACCCCCCCCCGGGAAAAAAAAAGGGGGTTTTCTTTTTTTGGGGGGCGCCTCTTCCCCTTTTCTCCTCCCTCCACAAAAAATCTCCTGTGTGCCCCTCGGGTGTCTTTTCGGGGGTGGGGGAAAAAAAAGGTGTTTCTCTCCTCCCCCCCCCCACAAGAGGGGGGGGGGTAAAAATAGGGGTTTTTCCCCCAAAAAATATCTGGGGGGGAGTAATAAAACCCCCCGGGAAAAAAAAAAAAATTTTTTTTTTCATCAAAAGAGGGCGCCGCTCCACAACAAGGGGGCGCAAAAAAAAAACTCTTATAAAAAGGGCGGCGCCT RQ $&&+&)&))+)('+'$&.3AAAA??==736).*()1244=A@8;.&028=@A6:@;?==;>=:5==?AA?A?@A@DG<AAE?===:8DA@???D===@?AAA??==CBA???A@;<</752:=@AAAA=@;2;52@?@AAAA@@@@CEBAAAAAAAAAACAA9?@>AACC????:;@<<<CD5;:*:7:??AAAAA??D??@AAA@AA@@@@DDAAA@A@@?::>8;788*/*461@96/725;>9<=,+-!+*,B=>A97<)1.*7640/),/*./,)2--16,41/6.315:8>D@AAA;9/()533*/39==;:12866<775886?C;;8A8;13-192;,6;9?C>4;=@B@@E8553????7:059/+/-,,,.02638:6931342::==811,.,2,5/,-2)*/1**0,,)*.*,**)*55494=>?65*31%**,017535/-%*/.5/*,,*2,*,%+++2&-)*+1)+50746.**2,,/*+2***2/-*5*)*+05201,))1+))%()$.2.)0.1-%12;00-*,*7**028<6:8,2-()())))(**+0/6/66>B52..-*55;71.-*)0/,-*+5++*1**'*,51**)+&++***,.=503-,*(3+)25).5/,)++,'%))))(+)++2,,+((),,0+)))))(((,)()))).1/./0*-/,4**,,0/6+,())),.,)$**+/37-1,.))(+)')/+*..00+)(.-).22*%0642**.+33+**)),+)**/(**,*))),+)+*-,+()+(*+1+))))(,-++$(-++)+.1*)))(&*)++,+*+032*-(%)&*(*,,,)+$))))*..6/.+))$+)%(&/&*),+.+),,)))'(,*(**)(++*2*+).)),9@A1,)****,****+))&)')%+-/20,034/+)-1-/++,--)20-1)**)(1--+-(')+*+$)),)+240,,-+-+*+))1)*))*,*).+,+*-+,200--5))++))694-10760-)++)*-0-*+)(-+(**--/*)**))')))+0))**+'+**)'''&*-,*+*&)**+-//1-*++/+*,***-+-,202+))./1**2),+,39;52,,*+)))+)/65*))''))7797-**(*()**-,--//2/***..*79,&*&(($)--+.,*,*---30C*))()))+(*)+)(&)*())))***2 TN 230375a_3 QL 16 QR 447 RT HAF3 17 33 = MIRA . RT HAF5 34 60 = MIRA . RT KMRF 41 81 = MIRA . RT HAF3 61 61 = MIRA . RT HAF5 62 136 = MIRA . RT HAF4 137 142 = MIRA . RT HAF5 143 248 = MIRA . RT KMRF 168 197 = MIRA . RT KMRF 202 222 = MIRA . RT SRMr 211 211 = MIRA . RT KMRF 229 249 = MIRA . RT HAF4 249 260 = MIRA . RT HAF3 306 316 = MIRA . RT KMRF 316 336 = MIRA . RT HAF5 317 363 = MIRA . RT KMRF 344 364 = MIRA . RT HAF3 364 419 = MIRA . RT HAF5 421 444 = MIRA . RT HAF3 445 447 = MIRA . ER AT 23 454 16 447 RD 230375b.t1 RG 1 RS CAATGGATCCTTGAATCTTCTACACCTCCCCCTCCTCTGAAACAGCTGCCTTAGCTTCAGGAAC*TCGAGTACTGTGGGCAATTTAGAAAAAgAACATGCAGTTTGAAATTCTGAATTTGCAAAGTACTGTAAGAATAATTTATAGTAATGAGTTTAAAAATCAACTTTTTATTGCCTTCTCACCAGCTGCAAAGTGTTTTGTACCAGTGAATTTTTGCAATAATGCAGTATGGTACATTTTTCAACTTTGAATAAAGAATACTTGAACTTGAAAAAAAAATAAAAAACCAAAAGATCTTTATTTAACCGGCCGCAAGCTTATTCCCTTTAGTGAGGGTTAATTTTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX RQ ''*,32+,1/4(&)(033,.51,(*01B=@@=AA?@@BB>=383*>8<A:628<=;6<<3..'*,//86*443=????AAAA@A8;,;82;2!2.4:;AAAAAA:C:7??ABAA?????EE@@?????;BA1;4948==AAAA@@ABG?@????@A@>??A<A==DA@BA?D@@AAAA@@??>84.+;<AA@@CA>?>>C<@;@4;3*305:;;@=696554>@@?CC@==>===DAAAAAA@C><A=6;==98?<?>>1426==8:::<8-,'+,.A=92/-+39;+,*'2&))152435/8B?76)516>=B?>??C>>?BABEEAAAA4:33;=9@?A@=<43!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! TN 230375b_1 QL 25 QR 282 AO 1 64 1 64 AO 66 1235 65 1234 RT HAF3 25 37 = MIRA . RT HAF4 38 63 = MIRA . RT HAF5 64 155 = MIRA . RT KMRF 76 96 = MIRA . RT SRMr 87 87 = MIRA . RT KMRF 101 130 = MIRA . RT HAF4 156 161 = MIRA . RT HAF5 162 236 = MIRA . RT KMRF 217 269 = MIRA . RT HAF3 237 237 = MIRA . RT HAF5 238 266 = MIRA . RT HAF4 267 267 = MIRA . RT HAF3 268 279 = MIRA . ER AT 280 23 25 282 RD 230375b.t3 RG 1 RS CCGCGTTAATAAAGGCCTTTTTTAGGATTTTTTTTTTCAAGTTCCAGTATTcttTATtCAAAgTTGAAAAATGTACCATACTGCATTATTGCAAAAATTCACTGGTACAAAACACTTTGCAGCTGGTGAGAAGGCAATAAAAAGTTGATTTTTAAACTCATTACTATAAATTATTCTTACAGTACTTTGCAAATTCAGAATTTCAAACTGCATGTTCTTTTTCTAAATTGCCCACAGTACTCGAGGTTCCTGAAgCTAAGGCAGCTGTTTCACATGAAGGGGAAGAATGTACCAAATTTCATGGAATCCATTTCCCCTTCCAATGCCAAATAACTCCGAGGGCTCCACCCTGCCTTTTCTCTGTATCCAAAAAACCCCTCTCGCCCCCTCTCGGAATGGGCTCTCGTGGAAAAAAAACCATCCTCCTGTAATATCCCCGGTAAACCCATTAAAAATTTTCGGCAAAAACTTTGGTGGGATTTCCACGAAATATTTTGCAACAACCAATTTTTTGTCCCCCCCGGCGTGGTCTGCCCCCCACCCAAAACATCCCTTGCTCCTTGTTAAAAAAAAAACGCCCCCCCTTTTTTTTTTCCAACCCCCCCTCCCCTGCCCAAAATCCTGGGCCTCCGGAGAGGGCGCAAAACTCCCCCAAAGAGATAATAGATTCTTTTTAAATTTCCGTAAATCCCTTTTTCACACCTGTGTCTCCCTCGTTTTAAAAAGGGTTTACCCCCCCCACAATTTCTCCCCCCCTCCCTTTCTAAAAACCGGAAAACCCTATAATTTTTTAAACCCCGGGGGGGTGTCCCCAAAGTAGATTTTAACACTCACACCCCCCATTTAAATTGTGCCTTTGCCCCACACTGGGCCCGCCTCTTTCCAATTCGGGAAAAAACCTTTCTTTCCCCCCCCCTATTAATATGAATCCCGCCCACACCCCCGCGGGGAAAAAGAGAGAGGGTGTGGTGCAGTCTTTTGGGGCCCCCCTCCCCTCTCTCTCCCCCCACATAGAAATCCCCTGCGGCGCTCTGCGTGCTCTCTGGTGGCGGGAAAAGGTGTATACTCTCTCTCCTCCTCAAGGGGGGATATCGCACGTGTGTCCCCCTAATCTGCGGAGAGATACCCCGAGAGAGAATAAATTTATACATCATCAGGGGCCCCAATGGGGCAAACACGCCTATAGGGCGC RQ &%()()*1++/321((++-.1+&+'''&,?EE,AE?AA>665&,)((2/+%!!!)-+!023-!2.,3*6665,265=7=?AABABABB?=@===?;>;=@@ABAAA??AAA?=A:7<9D??A???AAAC18;6;;ACE@@@@B7<05;;?@A@A??@A?:<<A@@D@@AAA?>99?@:AAA@A?A?=;????AA599*848;AAA@AAAAB;:7:2;@?BBA??ACGGABCDA=??=;;==<,;24?7></9;5!50/24.13)4,.772-.%+&.,/0:;222),,'()$))),+.+*,,(,.,+)*-((0A1,2150*(**&+*,,,,+*)*-''-++*0,*,.323/*+)*)*+1())*)*+*..3;9/-/%(()))*,)-12/**.4.*-(()+*)5415((()(*03*)2**)*+()*,+)(),))()(**.-0+(+()()*()),.//*/<++-030.+*,**()-(*)*)()(*)))'(),,)++)(+*+*(*,()((),+5420*()+176;1./32-))((..()0/;8121-)**))(***-()*&),,)**(*)(36;@@@5/*))/-,;722335@@::6*+**3,,-,+*-*1***+))()(25,+,,++-2./-,+*..6-5-.,/+)*0+-..)+.4/*-,*''')))())*,*,+-+*,/.-*/4<+*,,+*(//,,0/-*()**(**++))+.)),*))),,23=3.+()(,***.34;96-*)*+,+24,,*,,15472-**935(250/1*-.***-10<2*+,++,.))),2+.=2?3/1832-+*/*))+*)')+'-())()(())''(&+()+,--.-**,1.:57,)),((*+23-1*.()((''()'**)+*(*,,-))***)+&(('+))21())(()))**-6-/.<1,**()))+0+,'&&(())*(()))))+0))),*(,()))+**,+*+%)(),+((+'(*)(''')145))(+,+,++,,,)**+*08...051..+.-,)+')((+)'()')+1***%(+--+/+))((()*),***,51./1,(*,11--1,,&%*)*,**(+++))'(7:/,,()(),&)'''(),))(),((()(&(,*/*),*-&**+)))),..2-+-+,,-,*)+(((,/,((((,+))(&+*0+*+))))(()+'''('))(.(*)*+++- TN 230375b_3 QL 29 QR 272 RT HAF3 29 45 = MIRA . RT HAF5 46 72 = MIRA . RT KMRF 53 93 = MIRA . RT HAF3 73 73 = MIRA . RT HAF5 74 148 = MIRA . RT HAF4 149 154 = MIRA . RT HAF5 155 260 = MIRA . RT KMRF 180 209 = MIRA . RT KMRF 214 234 = MIRA . RT SRMr 223 223 = MIRA . RT KMRF 241 261 = MIRA . RT HAF4 261 272 = MIRA . ER AT 24 267 29 272 // EC