annotate tools/samtools_idxstats/samtools_idxstats.xml @ 0:d4412c04d7b1 draft

Uploaded v0.0.1 (as tested previously on the Test Tool Shed)
author peterjc
date Wed, 20 Nov 2013 12:27:33 -0500
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children 8945bad80f4a
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1 <tool id="samtools_idxstats" name="BAM mapping statistics" version="0.0.1">
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2 <description>samtools idxstats</description>
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3 <requirements>
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4 <requirement type="binary">samtools</requirement>
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5 <requirement type="package" version="0.1.19">samtools</requirement>
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6 </requirements>
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7 <version_command interpreter="python">samtools_idxstats.py --version</version_command>
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8 <command interpreter="python">samtools_idxstats.py "$input_bam" "${input_bam.metadata.bam_index}" "$out_tabular"</command>
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9 <inputs>
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10 <param name="input_bam" type="data" format="bam" label="Input BAM file" />
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11 </inputs>
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12 <outputs>
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13 <data name="out_tabular" format="tabular" label="$input_bam.name (idxstats)" />
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14 </outputs>
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15 <stdio>
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16 <!-- Assume anything other than zero is an error -->
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17 <exit_code range="1:" />
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18 <exit_code range=":-1" />
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19 </stdio>
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20 <tests>
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21 <test>
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22 <param name="input_bam" value="ex1.bam" ftype="bam" />
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23 <output name="out_tabular" file="ex1.idxstats.tabular" ftype="tabular" />
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24 </test>
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25 </tests>
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26 <help>
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27 **What it does**
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28
d4412c04d7b1 Uploaded v0.0.1 (as tested previously on the Test Tool Shed)
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29 This tool runs the ``samtools idxstats`` command in the SAMtools toolkit.
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30
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31 Input is a sorted and indexed BAM file, the output is tabular with
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32 four columns (one row per reference sequence plus a final line for
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33 unmapped reads):
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34
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35 ====== =================================================================================
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36 Column Description
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37 ------ ---------------------------------------------------------------------------------
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38 1 Reference sequence identifier
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39 2 Reference sequence length
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40 3 Number of mapped reads
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41 4 Number of placed but unmapped reads (typically unmapped partners of mapped reads)
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42 ====== =================================================================================
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43
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44 Example output from a *de novo* assembly:
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45
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46 ========== ====== ====== =====
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47 contig_1 170035 98397 0
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48 contig_2 403835 199564 0
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49 contig_3 553102 288189 0
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50 ... ... ... ...
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51 contig_603 653 50 0
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52 contig_604 214 6 0
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53 \* 0 0 50320
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54 ========== ====== ====== =====
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55
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56 In this example there were 604 contigs, each with one line in the output table,
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57 plus the final row (labelled with an asterisk) representing 50320 unmapped reads.
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58 In this BAM file, the final column was otherwise zero.
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59
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60
d4412c04d7b1 Uploaded v0.0.1 (as tested previously on the Test Tool Shed)
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61 **Citation**
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62
d4412c04d7b1 Uploaded v0.0.1 (as tested previously on the Test Tool Shed)
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63 If you use this Galaxy tool in work leading to a scientific publication please
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64 cite:
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65
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66 Heng Li et al (2009). The Sequence Alignment/Map format and SAMtools.
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67 Bioinformatics 25(16), 2078-9.
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68 http://dx.doi.org/10.1093/bioinformatics/btp352
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69
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70 Peter J.A. Cock (2013), Galaxy wrapper for the samtools idxstats command
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71 http://toolshed.g2.bx.psu.edu/view/peterjc/samtools_idxstats
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72
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73 This wrapper is available to install into other Galaxy Instances via the Galaxy
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74 Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/samtools_idxstats
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75 </help>
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76 </tool>