Mercurial > repos > peterjc > tmhmm_and_signalp
annotate tools/protein_analysis/README @ 15:6abd809cefdd draft
Uploaded v0.2.4, added unit tests for Promoter 2
| author | peterjc | 
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| date | Thu, 25 Apr 2013 12:25:52 -0400 | 
| parents | 6365217cd3de | 
| children | 
| rev | line source | 
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changeset | 1 This package contains Galaxy wrappers for a selection of standalone command | 
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changeset | 2 line protein analysis tools: | 
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changeset | 3 | 
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changeset | 4 * SignalP 3.0, THMHMM 2.0, Promoter 2.0 from the Center for Biological | 
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changeset | 5 Sequence Analysis at the Technical University of Denmark, | 
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changeset | 6 http://www.cbs.dtu.dk/cbs/ | 
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changeset | 7 | 
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changeset | 8 * WoLF PSORT v0.2 from http://wolfpsort.org/ | 
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changeset | 9 | 
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changeset | 10 * PSORTb v3 from http://www.psort.org/downloads/index.html | 
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changeset | 11 | 
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changeset | 12 Also, the RXLR motif tool uses SignalP 3.0 and HMMER 2.3.2 internally. | 
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changeset | 13 | 
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changeset | 14 To use these Galaxy wrappers you must first install the command line tools. | 
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changeset | 15 At the time of writing they are all free for academic use, or open source. | 
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changeset | 16 | 
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changeset | 17 These wrappers are copyright 2010-2013 by Peter Cock, James Hutton Institute | 
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changeset | 18 (formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. | 
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changeset | 19 Contributions/revisions copyright 2011 Konrad Paszkiewicz. All rights reserved. | 
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changeset | 20 See the included LICENCE file for details (an MIT style open source licence). | 
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changeset | 21 | 
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changeset | 23 Requirements | 
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changeset | 24 ============ | 
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changeset | 25 | 
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changeset | 26 First install those command line tools you wish to use the wrappers for: | 
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changeset | 27 | 
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changeset | 28 1. Install the command line version of SignalP 3.0 and ensure "signalp" is | 
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changeset | 29 on the PATH, see: http://www.cbs.dtu.dk/services/SignalP/ | 
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changeset | 30 | 
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changeset | 31 2. Install the command line version of TMHMM 2.0 and ensure "tmhmm" is on | 
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changeset | 32 the PATH, see: http://www.cbs.dtu.dk/services/TMHMM/ | 
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changeset | 33 | 
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changeset | 34 3. Install the command line version of Promoter 2.0 and ensure "promoter" is | 
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changeset | 35 on the PATH, see: http://www.cbs.dtu.dk/services/Promoter | 
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changeset | 36 | 
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changeset | 37 4. Install the WoLF PSORT v0.2 package, and ensure "runWolfPsortSummary" | 
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changeset | 38 is on the PATH (we use an extra wrapper script to change to the WoLF PSORT | 
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changeset | 39 directory, run runWolfPsortSummary, and then change back to the original | 
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changeset | 40 directory), see: http://wolfpsort.org/WoLFPSORT_package/version0.2/ | 
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changeset | 41 | 
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changeset | 42 5. Install hmmsearch from HMMER 2.3.2 (the last stable release of HMMER 2) | 
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changeset | 43 but put it on the path under the name hmmsearch2 (allowing it to co-exist | 
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changeset | 44 with HMMER 3), or edit rlxr_motif.py accordingly. | 
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changeset | 45 | 
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changeset | 46 Verify each of the tools is installed and working from the command line | 
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changeset | 47 (when logged in as the Galaxy user if appropriate). | 
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changeset | 48 | 
| 13 | 49 | 
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changeset | 50 Manual Installation | 
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changeset | 51 =================== | 
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changeset | 52 | 
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changeset | 53 1. Create a folder tools/protein_analysis under your Galaxy installation. | 
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changeset | 54 This folder name is not critical, and can be changed if desired - you | 
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changeset | 55 must update the paths used in tool_conf.xml to match. | 
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changeset | 56 | 
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changeset | 57 2. Copy/move the following files (from this archive) there: | 
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changeset | 58 | 
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changeset | 59 tmhmm2.xml (Galaxy tool definition) | 
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changeset | 60 tmhmm2.py (Python wrapper script) | 
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changeset | 61 | 
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changeset | 62 signalp3.xml (Galaxy tool definition) | 
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changeset | 63 signalp3.py (Python wrapper script) | 
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changeset | 64 | 
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changeset | 65 promoter2.xml (Galaxy tool definition) | 
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changeset | 66 promoter2.py (Python wrapper script) | 
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changeset | 67 | 
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changeset | 68 psortb.xml (Galaxy tool definition) | 
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changeset | 69 psortb.py (Python wrapper script) | 
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changeset | 70 | 
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changeset | 71 wolf_psort.xml (Galaxy tool definition) | 
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changeset | 72 wolf_psort.py (Python wrapper script) | 
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changeset | 73 | 
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changeset | 74 rxlr_motifs.xml (Galaxy tool definition) | 
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changeset | 75 rxlr_motifs.py (Python script) | 
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changeset | 76 | 
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changeset | 77 seq_analysis_utils.py (shared Python code) | 
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changeset | 78 LICENCE | 
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changeset | 79 README (this file) | 
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changeset | 80 | 
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changeset | 81 3. Edit your Galaxy conjuration file tool_conf.xml (to use the tools) AND | 
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changeset | 82 also tool_conf.xml.sample (to run the tests) to include the new tools | 
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changeset | 83 by adding: | 
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changeset | 84 | 
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changeset | 85 <section name="Protein sequence analysis" id="protein_analysis"> | 
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changeset | 86 <tool file="protein_analysis/tmhmm2.xml" /> | 
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changeset | 87 <tool file="protein_analysis/signalp3.xml" /> | 
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changeset | 88 <tool file="protein_analysis/psortb.xml" /> | 
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changeset | 89 <tool file="protein_analysis/wolf_psort.xml" /> | 
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changeset | 90 <tool file="protein_analysis/rxlr_motifs.xml" /> | 
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changeset | 91 </section> | 
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changeset | 92 <section name="Nucleotide sequence analysis" id="nucleotide_analysis"> | 
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changeset | 93 <tool file="protein_analysis/promoter2.xml" /> | 
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changeset | 94 </section> | 
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changeset | 95 | 
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changeset | 96 Leave out the lines for any tools you do not wish to use in Galaxy. | 
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changeset | 97 | 
| 13 | 98 4. Copy/move the test-data files (from this archive) to Galaxy's | 
| 99 subfolder test-data. | |
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changeset | 100 | 
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changeset | 101 5. Run the Galaxy functional tests for these new wrappers with: | 
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changeset | 102 | 
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changeset | 103 ./run_functional_tests.sh -id tmhmm2 | 
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changeset | 104 ./run_functional_tests.sh -id signalp3 | 
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changeset | 105 ./run_functional_tests.sh -id Psortb | 
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changeset | 106 ./run_functional_tests.sh -id rxlr_motifs | 
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changeset | 107 | 
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changeset | 108 Alternatively, this should work (assuming you left the name and id as shown in | 
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changeset | 109 the XML file tool_conf.xml.sample): | 
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changeset | 110 | 
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changeset | 111 ./run_functional_tests.sh -sid Protein_sequence_analysis-protein_analysis | 
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changeset | 112 | 
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changeset | 113 To check the section ID expected, use ./run_functional_tests.sh -list | 
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changeset | 114 | 
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changeset | 115 6. Restart Galaxy and check the new tools are shown and work. | 
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changeset | 116 | 
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changeset | 117 | 
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changeset | 118 History | 
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changeset | 119 ======= | 
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changeset | 120 | 
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changeset | 121 v0.0.1 - Initial release | 
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changeset | 122 v0.0.2 - Corrected some typos in the help text | 
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changeset | 123 - Renamed test output file to use Galaxy convention of *.tabular | 
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changeset | 124 v0.0.3 - Check for tmhmm2 silent failures (no output) | 
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changeset | 125 - Additional unit tests | 
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Migrated tool version 0.0.5 from old tool shed archive to new tool shed repository
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changeset | 126 v0.0.4 - Ignore comment lines in tmhmm2 output. | 
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Migrated tool version 0.0.5 from old tool shed archive to new tool shed repository
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changeset | 127 v0.0.5 - Explicitly request tmhmm short output (may not be the default) | 
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Migrated tool version 0.0.6 from old tool shed archive to new tool shed repository
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changeset | 128 v0.0.6 - Improvement to how sub-jobs are run (should be faster) | 
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Migrated tool version 0.0.7 from old tool shed archive to new tool shed repository
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changeset | 129 v0.0.7 - Change SignalP default truncation from 60 to 70 to match the | 
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changeset | 130 SignalP webservice. | 
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Migrated tool version 0.0.8 from old tool shed archive to new tool shed repository
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changeset | 131 v0.0.8 - Added WoLF PSORT wrapper to the suite. | 
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Migrated tool version 0.0.9 from old tool shed archive to new tool shed repository
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changeset | 132 v0.0.9 - Added our RXLR motifs tool to the suite. | 
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Uploaded v0.1.0, which adds a wrapper for Promoter 2.0 (DNA tool) and enables use of Galaxy's <parallelism> tag for SignalP, TMHMM X Promoter wrappers.
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changeset | 133 v0.1.0 - Added Promoter 2.0 wrapper (similar to SignalP & TMHMM wrappers) | 
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Uploaded v0.1.0, which adds a wrapper for Promoter 2.0 (DNA tool) and enables use of Galaxy's <parallelism> tag for SignalP, TMHMM X Promoter wrappers.
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changeset | 134 - Support Galaxy's <parallelism> tag for SignalP, TMHMM & Promoter | 
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Uploaded v0.1.1 of the bundle, which fixes an error in the header of the tabular output produced for Promoter 2.0
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changeset | 135 v0.1.1 - Fixed an error in the header of the tabular output from Promoter | 
| 9 | 136 v0.1.2 - Use the new <stdio> settings in the XML wrappers to catch errors | 
| 137 - Use SGE style $NSLOTS for thread count (otherwise default to 4) | |
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Uploaded v0.1.3 with missing HMM file for RXLR tool included.
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changeset | 138 v0.1.3 - Added missing file whisson_et_al_rxlr_eer_cropped.hmm to Tool Shed | 
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Uploaded v0.2.0 which added PSORTb wrapper (written with Konrad Paszkiewicz)
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changeset | 139 v0.2.0 - Added PSORTb wrapper to the suite, based on earlier work | 
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Uploaded v0.2.0 which added PSORTb wrapper (written with Konrad Paszkiewicz)
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changeset | 140 contributed by Konrad Paszkiewicz. | 
| 13 | 141 v0.2.1 - Use a script to create the Tool Shed tar-ball (removed some stray | 
| 142 files accidentally included previously via a wildcard). | |
| 143 v0.2.2 - Include missing test files. | |
| 14 | 144 v0.2.3 - Added unit tests for WoLF PSORT. | 
| 15 | 145 v0.2.4 - Added unit tests for Promoter 2 | 
| 9 | 146 | 
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changeset | 147 | 
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changeset | 148 Developers | 
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changeset | 149 ========== | 
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changeset | 150 | 
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Migrated tool version 0.0.6 from old tool shed archive to new tool shed repository
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changeset | 151 This script and other tools are being developed on the following hg branch: | 
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Migrated tool version 0.0.6 from old tool shed archive to new tool shed repository
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changeset | 152 http://bitbucket.org/peterjc/galaxy-central/src/tools | 
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changeset | 153 | 
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changeset | 154 This incorporates the previously used hg branch: | 
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changeset | 155 http://bitbucket.org/peterjc/galaxy-central/src/seq_analysis | 
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changeset | 156 | 
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changeset | 157 For making the "Galaxy Tool Shed" http://community.g2.bx.psu.edu/ tarball use | 
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changeset | 158 the following command from the Galaxy root folder: | 
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changeset | 159 | 
| 13 | 160 $ ./tools/protein_analysis/make_tmhmm_and_signalp.sh | 
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changeset | 161 | 
| 13 | 162 This simplifies ensuring a consistent set of files is bundled each time, | 
| 163 including all the relevant test files. | 
