Mercurial > repos > proteore > proteore_clusterprofiler
annotate cluster_profiler.xml @ 2:a71792aa5a08 draft
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author | proteore |
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date | Wed, 07 Mar 2018 09:37:57 -0500 |
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rev | line source |
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1 <tool id="cluter_profiler" name="clusterProfiler" version="0.1.0"> |
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2 <description> |
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3 GO terms classification and enrichment analysis |
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4 </description> |
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5 <requirements> |
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6 <requirement type="package" version="3.4.1">R</requirement> |
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7 <requirement type="package" version="3.5.0">bioconductor-org.hs.eg.db</requirement> |
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8 <requirement type="package" version="3.5.0">bioconductor-org.mm.eg.db</requirement> |
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9 <requirement type="package" version="3.2.0">bioconductor-dose</requirement> |
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10 <requirement type="package" version="3.4.4">bioconductor-clusterprofiler</requirement> |
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11 </requirements> |
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12 <command detect_errors="exit_code"><![CDATA[ |
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13 Rscript "$__tool_directory__/GO-enrich.R" |
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14 #if $input.ids == "text" |
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15 --input_type="text" |
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16 --input="$input.text" |
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17 #else |
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18 --input_type="file" |
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19 --input="$input.file" |
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20 --ncol="$input.ncol" |
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21 --header="$input.header" |
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22 #end if |
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23 |
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24 --id_type="$idti.idtypein" |
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25 |
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26 --species="$species" |
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27 |
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28 #if $ggo.go_represent == "true" |
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29 --go_represent="true" |
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30 --level="$ggo.level" |
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31 #else |
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32 --go_represent="false" |
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33 #end if |
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34 |
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35 #if $ego.go_enrich == "true" |
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36 --go_enrich="true" |
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37 --pval_cutoff="$ego.pval" |
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38 --qval_cutoff="$ego.qval" |
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39 #else |
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40 --go_enrich="false" |
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41 #end if |
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42 |
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43 --onto_opt="$ontology" |
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44 |
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45 --text_output="$text_output" |
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46 ]]></command> |
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47 <inputs> |
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48 <conditional name="input" > |
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49 <param name="ids" type="select" label="Provide your identifiers" help="Copy/paste or ID list from a file (e.g. table)" > |
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50 <option value="text">Copy/paste your identifiers</option> |
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51 <option value="file">Input file containing your identifiers</option> |
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52 </param> |
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53 <when value="text" > |
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54 <param name="txt" type="text" label="Copy/paste your identifiers" help='IDs must be separated by spaces into the form field, for example: P31946 P62258' > |
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55 <sanitizer> |
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56 <valid initial="string.printable"> |
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57 <remove value="'"/> |
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58 </valid> |
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59 <mapping initial="none"> |
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60 <add source="'" target="__sq__"/> |
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61 </mapping> |
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62 </sanitizer> |
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63 </param> |
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64 </when> |
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65 <when value="file" > |
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66 <param name="file" type="data" format="txt,tabular" label="Choose a file that contains your list of IDs" help="" /> |
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67 <param name="header" type="boolean" checked="true" truevalue="true" falsevalue="false" label="Does your input file contain header?" /> |
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68 <param name="ncol" type="text" label="The column number of IDs to map" help='For example, fill in "c1" if it is the first column, "c2" if it is the second column and so on' /> |
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69 </when> |
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70 </conditional> |
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71 <conditional name="idti" > |
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72 <param name="idtypein" type="select" label="Select type/source of identifier of your list" help="Please see example of IDs in help section" > |
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73 <option value="Uniprot">UniProt accession number</option> |
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74 <option value="Entrez">Entrez Gene ID</option> |
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75 </param> |
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76 <when value="Uniprot"/> |
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77 <when value="Entrez"/> |
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78 </conditional> |
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79 <param name="species" type="select" label="Select a species" > |
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80 <option value="human">Human</option> |
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81 <option value="mouse">Mouse</option> |
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82 <option value="rat">Rat</option> |
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83 </param> |
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84 <conditional name="ggo"> |
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85 <param name="go_represent" type="boolean" checked="true" truevalue="true" falsevalue="false" label="Do you want to perform GO categories representation analysis?"/> |
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86 <when value="true"> |
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87 <param name="level" type="select" label="Level of the ontology at which the profile has to be built (the higher this number, the deeper the GO level)"> |
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88 <option value="1">1</option> |
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89 <option value="2">2</option> |
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90 <option value="3" selected="True">3</option> |
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91 </param> |
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92 </when> |
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93 <when value="false"/> |
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94 </conditional> |
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95 <conditional name="ego"> |
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96 <param name="go_enrich" type="boolean" checked="true" truevalue="true" falsevalue="false" label="Do you want to perform GO categories enrichment analysis?"/> |
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97 <when value="true"> |
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98 <param name="pval" type="float" value="0.01" label="P-value cut off"/> |
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99 <param name="qval" type="float" value="0.05" label="Q-value cut off"/> |
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100 </when> |
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101 <when value="false"/> |
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102 </conditional> |
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103 <!--conditional name="fun" > |
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104 <param name="go_function" type="select" display="checkboxes" multiple="true" label="Please select analyses to perform"> |
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105 <option value="ggo">GO categories representation</option> |
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106 <option value="ego">GO categories enrichment (compared to a background/reference)</option> |
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107 </param> |
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108 <when value="ggo" > |
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109 <param name="level" type="select" label="Level of the ontology at which the profile has to be built (the higher this number, the deeper the GO level)"> |
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110 <option value="1">1</option> |
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111 <option value="2">2</option> |
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112 <option value="3" selected="True">3</option> |
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113 </param> |
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114 </when> |
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115 <when value="ego" > |
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116 <param name="pval" type="float" value="0.01" label="P-value cut off"/> |
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117 <param name="qval" type="float" value="0.05" label="Q-value cut off"/> |
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118 </when> |
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119 </conditional--> |
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120 |
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121 <param name="ontology" type="select" display="checkboxes" multiple="true" label="Please select GO terms category"> |
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122 <option value="CC">Cellular Component</option> |
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123 <option value="BP">Biological Process</option> |
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124 <option value="MF">Molecular Function</option> |
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125 </param> |
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126 |
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127 |
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128 |
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129 </inputs> |
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130 <outputs> |
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131 <data name="text_output" format="tabular" label="clusterProfiler text output" /> |
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132 <collection type="list" label="clusterProfiler diagram outputs" name="output" > |
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133 <discover_datasets pattern="(?P<designation>.+\.png)" ext="png" /> |
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134 </collection> |
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135 </outputs> |
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136 <tests> |
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137 <test> |
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138 <conditional name="input"> |
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139 <param name="ids" value="file"/> |
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140 <param name="file" value="Lacombe_et_al_2017_OK.txt"/> |
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141 <param name="header" value="true"/> |
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142 <param name="ncol" value="c1"/> |
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143 </conditional> |
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144 <conditional name="idti"> |
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145 <param name="idtypein" value="Uniprot"/> |
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146 </conditional> |
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147 <param name="species" value="human"/> |
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148 <conditional name="ggo"> |
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149 <param name="go_represent" value="true"/> |
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150 <param name="level" value="3"/> |
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151 </conditional> |
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152 <conditional name="ego"> |
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153 <param name="go_enrich" value="false"/> |
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154 </conditional> |
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155 <param name="ontology" value="CC"/> |
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156 <output name="text_output" file="clusterProfiler_text_output.tabular"/> |
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157 <output_collection name="output"> |
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158 <element name="clusterProfiler_diagram_outputs__GGO.CC.png" file="clusterProfiler_diagram_outputs__GGO.CC.png" ftype="png"/> |
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159 </output_collection> |
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160 </test> |
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161 </tests> |
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162 <help><![CDATA[ |
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163 |
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164 **Galaxy component based on R package clusterProfiler (see ref below)** |
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165 |
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166 This component allows to perform GO enrichment-analyses. |
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167 Given a list of IDs, the tool either |
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168 (i) performs gene classification based on GO distribution at a specific level, or |
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169 (ii) calculates GO categories enrichment (over- or under-representation) for the IDs of the input list, |
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170 compared to a background (whole organism or user-defined list). |
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171 |
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172 **Input required** |
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173 |
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174 This component works with Gene ids (e.g : 4151, 7412) or Uniprot accession number (e.g. P31946). |
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175 You can copy/paste these identifiers or supply a tabular file (.csv, .tsv, .txt, .tab) where there are contained. |
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176 |
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177 |
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178 **Output** |
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179 |
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180 Text (tables) and graphics representing the repartition and/or enrichment of GO categories. |
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181 |
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182 **User manual / Documentation** of the clusterProfiler R package (functions and parameters): |
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183 https://bioconductor.org/packages/3.7/bioc/vignettes/clusterProfiler/inst/doc/clusterProfiler.html |
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184 (Very well explained) |
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185 |
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186 **Reference** |
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187 |
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188 clusterProfiler R package reference : |
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189 G Yu, LG Wang, Y Han, QY He. clusterProfiler: an R package for comparing biological themes among gene clusters. |
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190 OMICS: A Journal of Integrative Biology 2012, 16(5):284-287. |
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191 doi:[10.1089/omi.2011.0118](http://dx.doi.org/10.1089/omi.2011.0118) |
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192 |
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193 |
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194 **Galaxy integration** |
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195 |
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196 T.P. Lien Nguyen, Florence Combes, Yves Vandenbrouck CEA, INSERM, CNRS, Grenoble-Alpes University, BIG Institute, FR |
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197 |
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198 Sandra Dérozier, Olivier Rué, Christophe Caron, Valentin Loux INRA, Paris-Saclay University, MAIAGE Unit, Migale Bioinformatics platform |
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199 |
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200 This work has been partially funded through the French National Agency for Research (ANR) IFB project. |
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201 |
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202 Contact support@proteore.org for any questions or concerns about the Galaxy implementation of this tool. |
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203 |
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204 |
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205 ]]></help> |
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206 <citations> |
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207 </citations> |
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208 </tool> |