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planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 24535690aedb81353cf5e036dc4577022d9604ad
author public-health-bioinformatics
date Thu, 27 Oct 2022 19:13:25 +0000
parents 3ab9d37e547e
children
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3 ## Obtain original sequence data
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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5 ```
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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6 wget ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR176/049/SRR17619849/SRR17619849_1.fastq.gz
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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7 wget ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR176/049/SRR17619849/SRR17619849_2.fastq.gz
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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8 wget ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR179/045/SRR17907745/SRR17907745_1.fastq.gz
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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9 wget ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR179/045/SRR17907745/SRR17907745_2.fastq.gz
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
public-health-bioinformatics
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10 ```
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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12 ## Obtain kraken2/bracken database
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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14 large file ~38GB compressed, ~50GB uncompressed
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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16 ```
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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17 wget https://genome-idx.s3.amazonaws.com/kraken/k2_standard_20210517.tar.gz
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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18 ```
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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20 ## Run kraken2
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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22 ```
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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23 kraken2 --db k2_standard_20210517 --report --report SRR17619849_kraken2.txt --paired SRR17619849_1.fastq.gz SRR17619849_2.fastq.gz
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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24 kraken2 --db k2_standard_20210517 --report --report SRR17907745_kraken2.txt --paired SRR17907745_1.fastq.gz SRR17907745_2.fastq.gz
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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25 ```
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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27 ## Run bracken
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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29 ```
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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30 bracken -d k2_standard_20210517 -i SRR17619849_kraken2.txt -o SRR17619849_bracken_abundances.tsv -r 250 -l S
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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31 bracken -d k2_standard_20210517 -i SRR17907745_kraken2.txt -o SRR17907745_bracken_abundances.tsv -r 150 -l S
3ab9d37e547e "planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
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32 ```