Mercurial > repos > q2d2 > qiime2__dada2__denoise_single
annotate qiime2__dada2__denoise_single.xml @ 2:e7812ca89033 draft
planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 65e4952f33eb335528e8553150e9097e5ea8f556
author | q2d2 |
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date | Thu, 08 Jun 2023 19:35:16 +0000 |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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1 <?xml version='1.0' encoding='utf-8'?> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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2 <!-- |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 69da7976573cc07a363ac66bdacc9269d7cd3732
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3 Copyright (c) 2023, QIIME 2 development team. |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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4 |
dba6cccafa22
planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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5 Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause) |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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6 --> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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7 <!-- |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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8 This tool was automatically generated by: |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 65e4952f33eb335528e8553150e9097e5ea8f556
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9 q2galaxy (version: 2023.5.0) |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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10 for: |
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e7812ca89033
planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 65e4952f33eb335528e8553150e9097e5ea8f556
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11 qiime2 (version: 2023.5.1) |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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12 --> |
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e7812ca89033
planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 65e4952f33eb335528e8553150e9097e5ea8f556
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13 <tool name="qiime2 dada2 denoise-single" id="qiime2__dada2__denoise_single" version="2023.5.0+q2galaxy.2023.5.0.2" profile="22.05" license="BSD-3-Clause"> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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14 <description>Denoise and dereplicate single-end sequences</description> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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15 <requirements> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 65e4952f33eb335528e8553150e9097e5ea8f556
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16 <container type="docker">quay.io/qiime2/core:2023.5</container> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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17 </requirements> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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18 <version_command>q2galaxy version dada2</version_command> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 69da7976573cc07a363ac66bdacc9269d7cd3732
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19 <command detect_errors="exit_code">q2galaxy run dada2 denoise_single '$inputs'</command> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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20 <configfiles> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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21 <inputs name="inputs" data_style="paths"/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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22 </configfiles> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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23 <inputs> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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24 <param name="demultiplexed_seqs" type="data" format="qza" label="demultiplexed_seqs: SampleData[SequencesWithQuality | PairedEndSequencesWithQuality]" help="[required] The single-end demultiplexed sequences to be denoised."> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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25 <options options_filter_attribute="metadata.semantic_type"> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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26 <filter type="add_value" value="SampleData[SequencesWithQuality]"/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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27 <filter type="add_value" value="SampleData[PairedEndSequencesWithQuality]"/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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28 </options> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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29 <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['SampleData[PairedEndSequencesWithQuality]', 'SampleData[SequencesWithQuality]']</validator> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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30 </param> |
dba6cccafa22
planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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31 <param name="trunc_len" type="integer" value="" label="trunc_len: Int" help="[required] Position at which sequences should be truncated due to decrease in quality. This truncates the 3' end of the of the input sequences, which will be the bases that were sequenced in the last cycles. Reads that are shorter than this value will be discarded. If 0 is provided, no truncation or length filtering will be performed"/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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32 <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options"> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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33 <param name="trim_left" type="integer" value="0" label="trim_left: Int" help="[default: 0] Position at which sequences should be trimmed due to low quality. This trims the 5' end of the of the input sequences, which will be the bases that were sequenced in the first cycles."/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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34 <param name="max_ee" type="float" value="2.0" label="max_ee: Float" help="[default: 2.0] Reads with number of expected errors higher than this value will be discarded."/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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35 <param name="trunc_q" type="integer" value="2" label="trunc_q: Int" help="[default: 2] Reads are truncated at the first instance of a quality score less than or equal to this value. If the resulting read is then shorter than `trunc_len`, it is discarded."/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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36 <param name="pooling_method" type="select" label="pooling_method: Str % Choices('independent', 'pseudo')" display="radio"> |
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37 <option value="independent" selected="true">independent</option> |
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38 <option value="pseudo">pseudo</option> |
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39 </param> |
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40 <param name="chimera_method" type="select" label="chimera_method: Str % Choices('consensus', 'none', 'pooled')" display="radio"> |
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41 <option value="consensus" selected="true">consensus</option> |
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42 <option value="none">none</option> |
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43 <option value="pooled">pooled</option> |
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44 </param> |
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45 <param name="min_fold_parent_over_abundance" type="float" value="1.0" label="min_fold_parent_over_abundance: Float" help="[default: 1.0] The minimum abundance of potential parents of a sequence being tested as chimeric, expressed as a fold-change versus the abundance of the sequence being tested. Values should be greater than or equal to 1 (i.e. parents should be more abundant than the sequence being tested). This parameter has no effect if chimera_method is "none"."/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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46 <param name="allow_one_off" type="boolean" truevalue="__q2galaxy__::literal::True" falsevalue="__q2galaxy__::literal::False" label="allow_one_off: Bool" help="[default: No] Bimeras that are one-off from exact are also identified if the `allow_one_off` argument is True.If True, a sequence will be identified as bimera if it is one mismatch or indel away from an exact bimera."/> |
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47 <param name="n_threads" type="integer" value="1" label="n_threads: Int" help="[default: 1] The number of threads to use for multithreaded processing. If 0 is provided, all available cores will be used."/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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48 <param name="n_reads_learn" type="integer" value="1000000" label="n_reads_learn: Int" help="[default: 1000000] The number of reads to use when training the error model. Smaller numbers will result in a shorter run time but a less reliable error model."/> |
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49 <param name="hashed_feature_ids" type="boolean" truevalue="__q2galaxy__::literal::True" falsevalue="__q2galaxy__::literal::False" checked="true" label="hashed_feature_ids: Bool" help="[default: Yes] If true, the feature ids in the resulting table will be presented as hashes of the sequences defining each feature. The hash will always be the same for the same sequence so this allows feature tables to be merged across runs of this method. You should only merge tables if the exact same parameters are used for each run."/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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50 </section> |
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51 </inputs> |
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52 <outputs> |
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53 <data name="table" format="qza" label="${tool.name} on ${on_string}: table.qza" from_work_dir="table.qza"/> |
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54 <data name="representative_sequences" format="qza" label="${tool.name} on ${on_string}: representative_sequences.qza" from_work_dir="representative_sequences.qza"/> |
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55 <data name="denoising_stats" format="qza" label="${tool.name} on ${on_string}: denoising_stats.qza" from_work_dir="denoising_stats.qza"/> |
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56 </outputs> |
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57 <tests> |
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58 <test> |
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59 <param name="demultiplexed_seqs" value="denoise_single.test0.demux-single.qza" ftype="qza"/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 69da7976573cc07a363ac66bdacc9269d7cd3732
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60 <param name="trunc_len" value="120"/> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 69da7976573cc07a363ac66bdacc9269d7cd3732
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61 <param name="trim_left" value="0"/> |
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62 <output name="representative_sequences" ftype="qza"> |
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planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__dada2 commit 69da7976573cc07a363ac66bdacc9269d7cd3732
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63 <assert_contents> |
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64 <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> |
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65 <has_line_matching expression="type: FeatureData\[Sequence\]"/> |
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66 </has_archive_member> |
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67 </assert_contents> |
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68 </output> |
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69 <output name="table" ftype="qza"> |
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70 <assert_contents> |
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71 <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> |
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72 <has_line_matching expression="type: FeatureTable\[Frequency\]"/> |
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73 </has_archive_member> |
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74 </assert_contents> |
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75 </output> |
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76 <output name="denoising_stats" ftype="qza"> |
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77 <assert_contents> |
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78 <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> |
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79 <has_line_matching expression="type: SampleData\[DADA2Stats\]"/> |
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80 </has_archive_member> |
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81 </assert_contents> |
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82 </output> |
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83 </test> |
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84 </tests> |
0
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85 <help> |
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86 QIIME 2: dada2 denoise-single |
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87 ============================= |
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88 Denoise and dereplicate single-end sequences |
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89 |
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90 |
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91 Outputs: |
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92 -------- |
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93 :table.qza: The resulting feature table. |
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94 :representative_sequences.qza: The resulting feature sequences. Each feature in the feature table will be represented by exactly one sequence. |
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95 :denoising_stats.qza: <no description> |
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96 |
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97 | |
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98 |
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99 Description: |
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100 ------------ |
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101 This method denoises single-end sequences, dereplicates them, and filters chimeras. |
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102 |
1
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103 Examples: |
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104 --------- |
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105 |
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106 denoise_single |
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107 ************** |
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108 Using the ``qiime2 dada2 denoise-single`` tool: |
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109 #. Set *"demultiplexed_seqs"* to ``#: demux-single.qza`` |
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110 #. Set *"trunc_len"* to ``120`` |
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111 #. Expand the ``additional options`` section |
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112 |
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113 - Leave *"trim_left"* as its default value of ``0`` |
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114 |
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115 #. Press the ``Execute`` button. |
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116 |
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117 |
0
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118 |
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119 | |
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120 |
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121 </help> |
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122 <citations> |
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123 <citation type="doi">10.1038/nmeth.3869</citation> |
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124 <citation type="doi">10.1038/s41587-019-0209-9</citation> |
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125 </citations> |
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126 </tool> |